Nghiên cứu sự biến đổi thành phần gen nhân ITS2, 28S rRNA và gen ty thể COX1 của loài sán lá gan lớn Fasciola sp dạng thuần và dạng lai gây bệnh trên động vật tại Việt Nam (LV thạc sĩ)

Nghiên cứu sự biến đổi thành phần gen nhân ITS2, 28S rRNA và gen ty thể COX1 của loài sán lá gan lớn Fasciola sp dạng thuần và dạng lai gây bệnh trên động vật tại Việt Nam (LV thạc sĩ)Nghiên cứu sự biến đổi thành phần gen nhân ITS2, 28S rRNA và gen ty thể COX1 của loài sán lá gan lớn Fasciola sp dạng thuần và dạng lai gây bệnh trên động vật tại Việt Nam (LV thạc sĩ)Nghiên cứu sự biến đổi thành phần gen nhân ITS2, 28S rRNA và gen ty thể COX1 của loài sán lá gan lớn Fasciola sp dạng thuần và dạng lai gây bệnh trên động vật tại Việt Nam (LV thạc sĩ)Nghiên cứu sự biến đổi thành phần gen nhân ITS2, 28S rRNA và gen ty thể COX1 của loài sán lá gan lớn Fasciola sp dạng thuần và dạng lai gây bệnh trên động vật tại Việt Nam (LV thạc sĩ)Nghiên cứu sự biến đổi thành phần gen nhân ITS2, 28S rRNA và gen ty thể COX1 của loài sán lá gan lớn Fasciola sp dạng thuần và dạng lai gây bệnh trên động vật tại Việt Nam (LV thạc sĩ)Nghiên cứu sự biến đổi thành phần gen nhân ITS2, 28S rRNA và gen ty thể COX1 của loài sán lá gan lớn Fasciola sp dạng thuần và dạng lai gây bệnh trên động vật tại Việt Nam (LV thạc sĩ)Nghiên cứu sự biến đổi thành phần gen nhân ITS2, 28S rRNA và gen ty thể COX1 của loài sán lá gan lớn Fasciola sp dạng thuần và dạng lai gây bệnh trên động vật tại Việt Nam (LV thạc sĩ)Nghiên cứu sự biến đổi thành phần gen nhân ITS2, 28S rRNA và gen ty thể COX1 của loài sán lá gan lớn Fasciola sp dạng thuần và dạng lai gây bệnh trên động vật tại Việt Nam (LV thạc sĩ)Nghiên cứu sự biến đổi thành phần gen nhân ITS2, 28S rRNA và gen ty thể COX1 của loài sán lá gan lớn Fasciola sp dạng thuần và dạng lai gây bệnh trên động vật tại Việt Nam (LV thạc sĩ)

i ĐẠI HỌC THÁI NGUYÊN TRƯỜNG ĐẠI HỌC KHOA HỌC - Nguyễn thị hoa ánh NGHIÊN CứU BIếN Đổi thành phần gen nhân its-2, 28s rrna gen ty thể cox1 loài sán gan lớn fasciola ssp dạng dạng lai gây bệnh động vật việt nam LUẬN VĂN THẠC SĨ KHOA HỌC CHUYÊN NGÀNH: CÔNG NGHỆ SINH HỌC THÁI NGUYÊN - 2016 i ĐẠI HỌC THÁI NGUYÊN TRƯỜNG ĐẠI HỌC KHOA HỌC - NGUYỄN THỊ HOA ÁNH NGHIÊN CỨU BIẾN ĐỔI THÀNH PHẦN GEN NHÂN ITS-2, 28S rRNA VÀ GEN TY THỂ COX1 CỦA LOÀI SÁN LÁ GAN LỚN FASCIOLA SSP DẠNG THUẦN VÀ DẠNG LAI GÂY BỆNH TRÊN ĐỘNG VẬT TẠI VIỆT NAM TÓM TẮT LUẬN VĂN THẠC SĨ CÔNG NGHỆ SINH HỌC Chuyên ngành: Công nghệ sinh học Mã số: 60 42 02 01 Người hướng dẫn khoa học: TS NGUYỄN THỊ BÍCH NGA THÁI NGUYÊN - 2016 i LỜI CAM ĐOAN Tôi xin cam đoan: Đây cơng trình nghiên cứu khoa học trực tiếp thực số kết cộng tác với cộng khác Các số liệu trình bày luận văn hồn tồn trung thực xác Ngày…… tháng … năm 201… Học viên Nguyễn Thị Hoa Ánh ii LỜI CẢM ƠN Tơi xin chân thành bày tỏ lòng biết ơn sâu sắc đến: - TS Nguyễn Thị Bích Nga người trực tiếp hướng dẫn, tạo điều kiện thuận lợi cho tơi suốt q trình nghiên cứu, học tập hoàn thành luận văn - Cảm ơn cán Phòng Miễn dịch học – Viện Công nghệ sinh học nơi thực tập nhiệt tình giúp đỡ tơi hồn thành luận văn Tôi xin gửi lời cảm ơn đến: - Ban Giám đốc Đại học Thái Nguyên, Ban Giám hiệu Trường Đại học Khoa học, Phòng Đào tạo Quan hệ Quốc tế, Khoa Khoa học sống - Trường Đại học Khoa học - Đại học Thái Nguyên - Sự tài trợ kinh phí Quỹ phát triển Khoa học công nghệ quốc gia (Nafosted) cho đề tài mã số 106-YS.02-2013.06 TS Nguyễn Thị Bích Nga làm chủ nhiệm - Cuối tơi muốn bày tỏ lòng biết ơn sâu sắc đến người thân khuyến khích,động viên chia sẻ khó khăn với tơi suốt q trình học tập hồn thành nghiên cứu Học viên Nguyễn Thị Hoa Ánh iii MỤC LỤC LỜI CAM ĐOAN i LỜI CẢM ƠN ii MỤC LỤC iii DANH MỤC CÁC CHỮ VIẾT TẮT v DANH MỤC BẢNG BIỂU vi DANH MỤC HÌNH ẢNH vii MỞ ĐẦU CHƯƠNG 1:TỔNG QUAN TÀI LIỆU 1.1 Giới thiệu loài sán gan lớn 1.1.1.Vị trí phân loại sán gan lớn hệ thống phân loại động vật 1.1.2.Đặc điểm hình thái học sán gan lớn Fasciola sp 1.1.3.Chu kỳ sinh trưởng phát triển sán 1.1.4.Trứng sán gan lớn 1.1.5.Sán trưởng thành 1.2.Đặc điểm bệnh sán gan lớn 10 1.2.1.Cơ chế sinh bệnh 10 1.2.2 Đặc điểm dịch tễ bệnh sán Fasciola trâu, bò Việt Nam 12 1.3 Tầm quan trọng nghiên cứu giám định loài phương pháp sinh học phân tử giải trình tự 17 1.3.1 Giới thiệu sơ hệ gen nhân ribosomal DNA (rDNA) 18 1.3.2 Hệ gen DNA ty thể 19 CHƯƠNG 2:VẬT LIỆU VÀ PHƯƠNG PHÁP NGHIÊN CỨU 22 2.1 Vật liệu nghiên cứu 22 2.2 Thời gian địa điểm nghiên cứu 22 2.3 Dụng cụ thiết bị hóa chất 22 2.3.1 Dụng cụ, trang thiết bị 22 2.3.2 Hóa chất 22 2.4 Phương pháp nghiên cứu 23 iv 2.4.1 Phương pháp thu thập bảo quản mẫu Fasciola sp 24 2.4.2 Phương pháp tách chiết DNA tổng số 24 2.4.3 Thiết kế mồi, thực PCR gen ITS2, 28S gen cox1 25 2.4.4 Phương pháp giải trình tự 27 2.4.5 Phương pháp phân tích xử lý số liệu 28 CHƯƠNG 3:KẾT QUẢ NGHIÊN CỨU VÀ BÀN LUẬN 30 3.1 Thu nhận gen ITS-2 chẩn đoán phân biệt 30 3.1.1 Thu thập mẫu sán gan lớn phân loại hình thái 30 3.1.2 Sử dụng gen ITS-2 chẩn đoán phân biệt 30 3.2 Kết thu nhận gen 28s xây dựng phả hệ 35 3.3 Kết thu nhận chuỗi gen ty thể COX1 39 3.3.1 Kết giải trình tự gen cox1 Fasciola sp Việt Nam so sánh với loài thuộc lớp sán Trematoda 41 3.3.2 Xây dựng mối quan hệ phả hệ loài thuộc lớp sán 47 KẾT LUẬN 53 TÀI LIỆU THAM KHẢO 54 v DANH MỤC CÁC CHỮ VIẾT TẮT DNA Deoxyribonucleotide AST Abnormal Spermatogenic Type NST Normal Spermatogenic Type Cox1 cytochrome oxidase subunit ITS-2 Second internal transcribed spacer Rdna Ribosome DNA SLGL Sán gan lớn PCR Polymerase chain reaction ddNTP dideoxyrinonucleotide triphosphate dNTP deoxyrinonucleotide triphosphate bp Cặp bazơ (base pair) TAE Tris base-Acetic-EDTA PAGE Điện di gel polyacylamid EtBr Ethidium Bromide EDTA Ethylene Diamine Tetraacemic Acid OD Mật độ quang học Kb Kilobase Kda Kilodalton µl Microlitre µm Micrometer mm Millimeter vi DANH MỤC BẢNG BIỂU Bảng 2.1 Danh sách các mồ i dùng phản ứng PCR thu nhận gen ITS-2, 28S COX1 25 Bảng 3.1 So sánh nucleotide vị trí biến đổi vùng giao gen ITS-2 mẫu sán gan lớn vùng địa lý vật chủ khác 33 Bảng 3.2 Các mẫu cung cấp chuỗi gen 28S nghiên cứu 36 Bảng 3.3 Các mẫu sán Trematoda Ngân hàng gen cung cấp chuỗi cox1 sử dụng so sánh đối chiếu nghiên cứu 40 Bảng 3.4 Tỷ lệ tương đồng trình tự amino acid gen cox1 loài sán thuộc lớp Trematoda 46 vii DANH MỤC HÌNH ẢNH Hình 1.1 Hình ảnh mơ tả hình thái sán gan lớn Fasciola sp Hình 1.2 Chu kỳ sinh trưởng phát triển SLGL Fasciola sp ký sinh vật chủ động vật người Hình 1.3 Hình ảnh trứng sán gan lớn Fasciola sp phóng đại 400 lần kính hiển vi điện tử Hình 1.4 Hình thể sán gan lớn Fasciola sp trưởng thành Hình 1.5 Mơ hình cấu trúc ribosome DNA gen nhân vùng gen cần nghiên cứu 18 Hình 1.6 Mơ hình cấu trúc DNA hệ gen ty thể 20 Hình 2.1 Sơ đồ quy trình nghiên cứu giải mã hệ gen ty thể Fasciola sp 23 Hình 3.1 Hình ảnh sán gan lớn FspNB-VN FspCB-VN nghiên cứu 30 Hình 3.2 Điện di kiểm tra sản phẩm PCR vùng gen ITS-2 31 Hình 3.3 So sánh trình tự chuỗi nucleotide ITS-2của 16 mẫu Fasciola sp 32 Hình 3.4 Điện di sản phẩm PCR gen 28S với cặp mồi U28SF – U28SR 35 Hình 3.5 Mối quan hệ nguồn gốc phả hệ mẫu Fasciola sp xác lập dựa thành phần nucleotide gen 28S ribosome chương trình MEGA6.06 38 Hình 3.6 Điện di kiểm tra sản phẩm PCR gen cox1 với cặp mồiFSP12F – FGHR2 40 Hình 3.7 Trình tự amino acid suy diễn FspNB-VN FspCB-VN so sánh với chuỗi gen cox1 thuộc lớp sán Trematoda 44 Hình 3.8 Cây phả hệ thể mối quan hệ loài mẫu sán dựa thành phần amino acid gen cox1 chương trình MEGA6 48 MỞ ĐẦU Lý chọn đề tài Bệnh sán gan lớn (fascioliasis) hai lồi Fasciola hepatica Fasciola gigantica thuộc họ Fasciolidaekí sinh động vật nhai lại (trâu, bò, dê, cừu…) dần thích ứng gây bệnh người Trước đây, đặc tính hình thái học (morphology), sinh thái học (ecology) huyết học (serology) thường sử dụng chẩn đoán đáp ứng phần lớn yêu cầu phân loại sinh vật có ngành ký sinh trùng [5],[18] Tuy nhiên phân loại sán gan dựa vào hình thái khó phân định Đặc biệt có tượng lai dạng trung gian (hybrid form) quần thể sán gan phát hiện, hệ gen có trộn lẫn F hepatica F gigantica (Fh/Fg), hệ gen nhân tế bào mang gen từ dòng bố (F hepatica) hệ gen ty thể (F gigantica) mang tính di truyền theo dòng mẹ [7] Bệnh sán gan lớn coi bệnh ký sinh trùng bị lãng quên (neglected emerging infectious disease) tổ chức y tế giới (WHO) quan tâm Sán gan lớn gây bệnh người, ảnh hưởng đến sức khỏe cộng đồng, chúng gây tổn thương bệnh lý hệ thống gan mật, gây can xi hóa đường mật, ký sinh lạc chỗ di chuyển lạc lên não… gây tổn thương nặng nhu mô gan làm suy gan dẫn đến tử vong [4], [7], [17] Một số trường hợpdo bệnh sán gan lớn người gây phản ứng viêm, xơ hóa gan, dẫn đến chẩn đốn bị nhầm bệnh ung thư gan [2], [5] Đối với động vật ăn cỏ (trâu, bò, dê, cừu…) nhiễm sán gan lớn làm giảm trọng lượng vật, ốm yếu, thiếu máu bệnh lý, vật không sinh sản, gây thiệt hại lớn kinh tế [11] Vì việc phát sớm xác định xác lồi Fasciola gây bệnh cần thiết, có ý nghĩa thực tiễn chẩn đoán điều trị Chẩn đốn, giám định phân biệt lồi Fasciola thực phương pháp sinh học phân tử dựa thị gen nhân ty thể 57 Japan on the basis of the sequences of ribosomal and mitochondrial DNA”, Parasitolology, 131, pp 679-685 39 Itagaki T, Sakaguchi K, Terasaki K, Sasaki O, Yoshihara S, Van Dung T (2009), “Occurrence of spermic diploid and aspermic triploid forms of Fasciola in Vietnam and their molecular characterization based on nuclear and mitochondrial DNA”, Parasitol Int, 58, pp 81-85 40 Jogen Hansen and Brian perry (1994), The epidemiology, diagnosis and control of Helminth parasites of Ruminants, Hand bock, pp 32 - 33 41 Kaufmann J (1996), Parasitic infection of domestic animal, Birkhauser verlag, Basel, Boston, Berlin, pp 90 – 94 42 Kramer F, Schnieder T (1998), “Sequence heterogeneity in a repetitive DNA element of Fasciola”, Int J Parasitol, 28(12), pp 1923-1929 43 Le TH, Blair D and McManus DP (2002), “Mitochondrial genomes of parasitic flatworms”, Trends in Parasitology, 18, pp 206-213 44 Le TH, Blair D, McManus DP (2000a), “Mitochondrial genomes of human helminths and their use as markers in population genetics and phylogeny”, Acta Tropica, 77(3), p 243-256 45 Le TH, Blair D, McManus DP (2001), “Complete DNA sequence and gene organization of the mitochondrial genome of the liver fuke, Fasciolahepatica L (Platyhelminthes; Trematoda)”, Parasitology, 123(6), pp 609-621 46 Le TH, De NV, Agatsuma T, Blair D, Vercruysse J, Dorny P, Nguyen TGT, and McManus DP (2007), “Molecular confirmation that Fasciola gigantica can undertake aberrant migrations in human hosts”, Journal of Clinical Microbiology, 45(2), pp 648 – 650 47 Le TH, Van De N, Agatsuma T, Nguyen TGT, Nguyen QD, McManus DP and Blair D (2008), “Human fascioliasis and the presence of hybrid/ introgressed forms of Fasciola hepatica and Fasciola gigantica in Vietnam”, Int J Parasitol, 38, pp 725 – 730 58 48 Le TH, Blair D, Agatsuma T, Humair PF, Campbell NJ, Iwagami M, Littlewood, DT, Peacock B, Johnston DA, Bartley J, Rollinson D, Herniou EA, Zarlenga DS and McManus DP (2000b), “Phylogenies inferred from mitochondrial gene orders-a cautionary tale from the parasitic flatworms”, Mol Biol Evol,17(7), pp 1123-1125 49 Liu F, Chen P, Cui SJ, Wang ZQ, Han ZG (2008), “SjTPdb: integrated transcriptome and proteome database and analysis platform for Schistosoma japonicum”, BMC Genomics,9, pp 304 50 Liu GH, Gasser RB, Young ND, Song HQ, Ai L and Zhu XQ (2014), “Complete mitochondrial genomes of the ‘intermediate form’ of Fasciola and Fasciola gigantica, and their comparison with F hepatica”, Parasites & Vectors, 7, pp 150 51 Lotfy WM, Brant SV, DeJong RJ, Le TH, Demiaszkiewicz A, et al (2008), “Evolutionary origins, diversification, and biogeography of liver flukes (Digenea, Fasciolidae)”, Am J Trop Med Hyg,79, pp 248–255 52 Mas-Coma S, Valero MA, Bargues MD (2009), “Fasciola, lymnaeids and human fascioliasis with a global overview on disease transmission, epidemiology, evolutionary genectics, molecular epidemiology and control”, Adv Parasitol, 69, pp 41-146 53 Nguyen ST, Nguyen DT, Van Nguyen T, Huynh VV, Le DQ, Fukuda Y, Nakai Y (2012), “Prevalence of Fasciola in cattle and of its intermediate host Lymnaea snails in central Vietnam”, Trop Anim Health Prod, 44(8), pp 1847-1853 54 Nguyen TG, Le TH, Dao TH, Tran TL, Praet N, Speybroeck N, Vercruysse J, Dorny P (2011), “Bovine fasciolosis in the human fasciolosis hyperendemic Binh Dinh province in Central Vietnam”, Acta Trop,117(1), pp 19-22 55 Nguyen TGT, De NV, Vercruysse J, Dorny P, Le TH (2009), “Genotypic characterization and species identification of Fasciola spp with implications regarding the isolatesinfecting goats in Vietnam”, Exp Parasitol, 123, pp 354-361 59 56 Nicholas KB anh HB Nicholas (1999), GeneDoc: a tool for editing and annotating multiple sequence aglignments, Distributed by authors 57 Okamoto M, Nakao M, Blair D, Anantaphruti MT, Waikagul J, Ito A (2010), “Evidence of hybridization between Taenia saginata and Taenia asiatica”, Parasitol Int, 59(1), pp 70-74 58 Soulsby EJL (1982), Helminth, Arthropod an protozoa of domestic animal, lea, Febiger – Philadelphia, pp 40 – 71 59 Steinauer ML, Hanelt B, Mwangi IN, Maina GM, Agola LE, Kinuthia JM, Mutuku MW, Mungai BN, Wilson WD, Mkoji GM, Loker ES (2008),“Introgressive hybridization of human and rodent schistosome parasites in western Kenya”, Mol Ecol,17(23), pp 5062-5074 60 Szymanski M, Barciszewska MZ, Barciszewski J, and ErdmannVA(2000), “5S ribosomal RNA database Y2K”, Nucleic Acids Res, 28, pp 166 – 167 61 Tamura K, Stecher G, Peterson D, Filipski A, and Kumar S (2013),“MEGA6: Molecular Evolutionary Genetics Analysis Version 6.0”, Mol Biol Evol, 30, pp 2725-2729 62 Van de peer Y, Chapelle S, De Wachter R (1996), “A quantitative map of nucleotide substitution rates in bacterial rRNA”, Nucleic Acids Res,24(17), pp 3381-3391 63 Walker SM, Prodöhl PA, Hoey EM, Fairweather I, Hanna RE, Brennan G, Trudgett A(2012), “Substantial genetic divergence between morphologically indistinguishable populations of Fasciola suggests the possibility of cryptic speciation”, Int J Parasitol,42(13-14), pp 1193-1199 64 Wannasan A, Khositharattanakool P, Chaiwong P, Piangjai S, Uparanukraw P, Morakote N (2014), “Identification of Fasciola species based on mitochondrial and nuclear DNA reveals the co-existence of intermediate Fasciola and Fasciola gigantica in Thailand”, Exp Parasitol,146, pp 64-70 65 Xuan Le T, Hung NT, Waikagul J (2005), “Cutaneous fascioliasis: a case report in Vietnam”, Am J Trop Med Hyg, 72(5), pp 508 - 509 60 66 Zarowiecki MZ, Huyse T, Littlewood DT (2007), “Making the most of mitochondrial genomes-markers for phylogeny,molecular ecology and barcodes in Schistosoma (Platyhelminthes: Digenea)”, Int J Parasitol, 37(12), pp 140118 Tài liệu dịch 67 Skrjabin KI Petrov AM (1977), Ngun lý mơn giun tròn thú y, Bùi Lập Đoàn Thị Băng Tâm dịch từ nguyên tiếng Nga, Nhà xuất khoa học kỹ thuật, Hà Nội, (1), tr 56 – 57 Tài liệu từ mạng internet 68 Hồ Việt Mỹ, Lê Quang Hùng, Trần Thị Kim Dung, Đặng Tất Thế (2000), http://www.dostbinhdinh.org.vn/kyYeu/KyyeuSTKT19972007/Giaidoan2002-2003/P101 - 102.htm 69 http://iranhelminthparasites.com/species/fasciola.htm 70 Sách “Ký sinh trùng côn trùng y học, Học viện Quân y, 2008 71 www.dpd.cdc.gov/dpdx Viện sốt rét kí sinh trùng trùng Trung ương 61 PHỤ LỤC Phụ lục 1.Trình tự nucleotide gen 28S FspNB-VN FspCB-VN FgigSN-AY2 FgigTH-HM0 FgigKE-EU0 FgET FgEQB FhEQB FspEQO FhAus FhBe FhepEG-EU0 FhepSA-AY2 FjacLK-EU0 FmagUS-EU0 FbusVN-EU0 : : : : : : : : : : : : : : : : * 20 * 40 * 60 * AATGAGGGTGAAAAGCCCCGTGGGGGTGGAGATTCAGAATGGCCCAGTATCTTCCTGAGCAGACCTTGGAGTCGG AATGAGGTTAAAAGCCCCGTGGGGGTGGAGATTCAGAATGGCCCAGTATCTTCTTGAGCAGACCTTGGAGTCGGG AATGAGGGTGAAAGGCCCGTGGGGGTGGAGATTCAGAATGGCCAGTATCTTCCTGAGCGGACCTTGGAGTCGGGT AATGAGGGTGAAAGGCCCGTGGGGGTGGAGATTCAGAATGGCCAGTATCTTCCTGAGCAGACCTTGGAGTCGGGT AATGAGGGTGAAAGGCCCGTGGGGGTGGAGATTCAGAATGGCCAGTATCTTCCTGAGCGGACCTTGGAGTCGGGT AATGAGGGTGAAAGGCCCGTGGGGGTGGAGATTCAGAATGGCCCAGTATCTTCTTGAGCGGACCTTGGAGTCGGG AATGAGGGTGAAAGGCCCCGTGGGGGTGGAGATTCAGAATGGCCAGTATCTCCCTGAGCAGACCTTGGAGTCGGG AATGAGGGTGAAAGGCCCCGTGGGGGTGGAGATTCAGAATGGCCCAGTATCTTCCCTGAGCAGACCTTGGAGTCG AATGAAGGTGAAAGGCCCCGTGGGGGTGGAGATTCAGGATGGCCCAGTATCTTCCTGAGCAGACCTTGGAGTCGG AATGAAGGGGAAAAGGCCCCGTGGGGGTGGAGATTCAGAATGGCCCAGTATCTTCCTGAGCAGACCTTGGAGTCG AATGAGGGTGAAAAGGCCCCGTGGGGGTGGAGATTCAGAATGGCCCAGTATCTTCCCTGAGCAGACCTTGGAGTC AATGAGGGTGAAAGGCCCGTGGGGGTGGAGATTCAGAATGGCCAGTATCTTCCTGAGCAGACCTTGGAGTCGGGT AATGAGGGTGAAAGGCCCGTGGGGGTGGAGATTCAGAATGGCCAGTATCTTCCTGAGCAGACCTTGGAGTCGGGT ATTGAGGGTGAAAGGCCCGTGGGGGTGGAGATTCAGAATGGCCAGTATCTTCCTGAGCAGACCTTGGAGTCGGGT AGTGAGGGTGAAAGGCCCGTGGGGGTGGAGATTCAGAATGGCCAGTATCTTCCTGAGCAGACCTTGGAGTCGGGT AGTGAGGGTGAAAGGCCCGTGGGGGTGGAGACTCAGATTGGACAGTATCTCCCTGAGCAGACCTTGGAGTCGGGT : : : : : : : : : : : : : : : : 75 75 75 75 75 75 75 75 75 75 75 75 75 75 75 75 FspNB-VN FspCB-VN FgigSN-AY2 FgigTH-HM0 FgigKE-EU0 FgET FgEQB FhEQB FspEQO FhAus FhBe FhepEG-EU0 FhepSA-AY2 FjacLK-EU0 FmagUS-EU0 FbusVN-EU0 : : : : : : : : : : : : : : : : 80 * 100 * 120 * 140 * GTTGTTTGTGAATGCAGCCC-AAAGCGGGTGGTAAACTCCATCCAAAGGCTAAATACTAGCACGAGTCCGATAGC TTGTTTGTGAATGCAGCCC-AAAGCGGGTGGTAAACTCCATCCCAAGGCTAAATACTAGCACGAGTCCGATAGCG TGTTTGTGAATGCAGCCC-AAAGCGGGTGGTAAACTCCATCC-AAGGCTAAATACTAGCACGAGTCCGATAGCGA TGTTTGTGAATGCAGCCC-AAAGCGGGTGGTAAACTCCATCC-AAGGCTAAATACTAGCACGAGTCCGATAGCGA TGTTTGTGAATGCAGCCC-AAAGCGGGTGGTAAACTCCATCC-AAGGCTAAATACTAGCACGAGTCCGATAGCGA TTGTTTGTGAATGCAGCCCAAAAGCGGGTGGTAAACTCCATCCCAAGGCTAAATACTAGCACGAGTCCGATAGCG TTGTTTGTGAATGCAGCCC-AAAGCGGGTGGTAAACTCCATCCCAAGGCTAAATACTAGCACGAGTCCGATAGCG GGTTGTTTGTGAATGCAGCCCCAAAGCGGGTGGTAAACTCCATCCCAAGGCTAAATACTAGCACGAGTCCGATAG GTTGTTTGTGAATGCAGCCCAAAAGCGGGTGGTAAACTCCATCCCAAGGCTAAATACTAGCACGAGTCCGATAGC GGTTGTTTGTGAATGCAGCCCAAAAGCGGGTGGTAAACTCCATCCCAAGGCTAAATACTAGCACGAGTCCGATAG GGGTTGTTTGTGAATGCAGCCCCAAAGCGGGTGGTAAACTCCATCCCAAGGCTAAATACTAGCACGAGTCCGATA TGTTTGTGAATGCAGCCC-AAAGCGGGTGGTAAACTCCATCC-AAGGCTAAATACTAGCACGAGTCCGATAGCGA TGTTTGTGAATGCAGCCC-AAAGCGGGTGGTAAACTCCATCC-AAGGCTAAATACTAGCACGAGTCCGATAGCGA TGTTTGTGAATGCAGCCC-AAAGCGGGTGGTAAACTCCATCC-AAGGCTAAATACTAGCACGAGTCCGATAGCGA TGTTTGTGAATGCAGCCC-AAAGCGGGTGGTAAACTCCATCC-AAGGCTAAATACTAGCACGAGTCCGATAGCGA TGTTTGTGAATGCAGCCC-AAAGTGGGTGGTAAACTCCATCC-AAGGCTAAATACTTGCACGAGTCCGATAGCGA : : : : : : : : : : : : : : : : 149 149 148 148 148 150 149 150 150 150 150 148 148 148 148 148 FspNB-VN FspCB-VN FgigSN-AY2 FgigTH-HM0 FgigKE-EU0 FgET FgEQB FhEQB FspEQO FhAus FhBe FhepEG-EU0 FhepSA-AY2 FjacLK-EU0 FmagUS-EU0 FbusVN-EU0 : : : : : : : : : : : : : : : : 160 * 180 * 200 * 220 GAACAAGTACCGTGAGGGAAAGTTGAAAAGTACTTTGAAGAGAGAGTAAACAGTGCGTGAAACCGTTCAGAGGTA AACAAGTACCGTGAGGGAAAGTTGAAAAGTACTTTGAAGAGAGAGTAAACAGTGCGTGAAACCGTTCAGAGGTAA ACAAGTACCGTGAGGGAAAGTTGAAAAGTACTTTGAAGAGAGAGTAAACAGTGCGTGAAACCGTTCAGAGGTAAA ACAAGTACCGTGAGGGAAAGTTGAAAAGTACTTTGAAGAGAGAGTAAACAGTGCGTGAAACCGTTCAGAGGTAAA ACAAGTACCGTGAGGGAAAGTTGAAAAGTACTTTGAAGAGAGAGTAAACAGTGCGTGAAACCGTTCAGAGGTAAA AACAAGTACCGTGAGGGAAAGTTGAAAAGTACTTTGAAGAGAGAGTAAACAGTGCGTGAAACCGTTCAGAGGTAA AACAAGTACCGTGAGGGAAAGTTGAAAAGTACTTTGAAGAGAGAGTAAACAGTGCGTGAAACCGTTCAGAGGTAA CGAACAAGTACCGTGAGGGAAAGTTGAAAAGTACTTTGAAGAGAGAGTAAACAGTGCGTGAAACCGTTCAGAGGT GAACAAGTACCGTGAGGGAAAGTTGAAAAGTACTTTGAAGAGAGAGTAAACAGTGCGTGAAACCGTTCAGAGGTA CGAACAAGTACCGTGAGGGAAAGTTGAAAAGTACTTTGAAGAGAGAGTAAACAGTGCGTGAAACCGTTCAGAGGT GCGAACAAGTACCGTGAGGGAAAGTTGAAAAGTACTTTGAAGAGAGAGTAAACAGTGCGTGAAACCGTTCAGAGG ACAAGTACCGTGAGGGAAAGTTGAAAAGTACTTTGAAGAGAGAGTAAACAGTGCGTGAAACCGTTCAGAGGTAAA ACAAGTACCGTGAGGGAAAGTTGAAAAGTACTTTGAAGAGAGAGTAAACAGTGCGTGAAACCGTTCAGAGGTAAA ACAAGTACCGTGAGGGAAAGTTGAAAAGTACTTTGAAGAGAGAGTAAACAGTGCGTGAAACCGTTCAGAGGTAAA ACAAGTACCGTGAGGGAAAGTTGAAAAGTACTTTGAAGAGAGAGTAAACAGTGCGTGAAACCGTTCAGAGGTAAA ACAAGTACCGTGAGGGAAAGTTGAAAAGTACTTTGAAGAGAGAGTAAACAGTGCGTGAAACCGTTCAGAGGTAAA : : : : : : : : : : : : : : : : 224 224 223 223 223 225 224 225 225 225 225 223 223 223 223 223 FspNB-VN FspCB-VN FgigSN-AY2 FgigTH-HM0 FgigKE-EU0 FgET FgEQB FhEQB FspEQO FhAus FhBe FhepEG-EU0 FhepSA-AY2 FjacLK-EU0 FmagUS-EU0 FbusVN-EU0 : : : : : : : : : : : : : : : : * 240 * 260 * 280 * 300 AACAGGTGGAGTTGAACTGCAAGCTCTGAGGATTCAGCTGGTGAGTATGGCATGAGCTTGGTCATATTGGTTGTG ACAGGTGGAGTTGAACTGCAAGCTCTGAGGATTCAGCTGGTGAGTATGGCATGAGCTTGGTCATATTGGTTGTGT CAGGTGGAGTTGAACTGCAAGCTCTGAGGATTCAGCTGGTGAGTATGGCATGAGCTTGGTCATATTGGTTGTGTG CAGGTGGAGTTGAACTGCAAGCTCTGAGGATTCAGCTGGTGAGTATGGCATGAGCTTGGTCATATTGGTTGTGTG CAGGTGGAGTTGAACTGCAAGCTCTGAGGATTCAGCTGGTGAGTATGGCATGAGCTTGGTCATATTGGTTGTGTG ACAGGTGGAGTTGAACTGCAAGCTCTGAGGATTCAGCTGGTGAGTATGGCATGAGCTTGGTCATATTGGTTGTGT ACAGGTGGAGTTGAACTGCAAGCTCTGAGGATTCAGCTGGTGAGTATGGCATGAGCTTGGTCATATTGGTTGTGT AAACAGGTGGAGTTGAACTGCAAGCTCTGAGGATTCAGCTGGTGAGTATGGCATGAGCTTGGTCATATTGGTTGT AACAGGTGGAGTTGAACTGCAAGCTCTGAGGATTCAGCTGGTGAGTATGGCATGAGCTTGGTCATATTGGTTGTG AAACAGGTGGAGTTGAACTGCAAGCTCTGAGGATTCAGCTGGTGAGTATGGCATGAGCTTGGTCATATTGGTTGT TAAACAGGTGGAGTTGAACTGCAAGCTCTGAGGATTCAGCTGGTGAGTATGGCATGAGCTTGGTCATATTGGTTG CAGGTGGAGTTGAACTGCAAGCTCTGAGGATTCAGCTGGTGAGTATGGCATGAGCTTGGTCATATTGGTTGTGTG CAGGTGGAGTTGAACTGCAAGCTCTGAGGATTCAGCTGGTGAGTATGGCATGAGCTTGGTCATATTGGTTGTGTG CAGGTGGAGTTGAACTGCAAGCTCTGAGGATTCAGCTGGTGAGTGTGGCATGAGCTTGGTCATGTGGGTTGTGTG CAGGTGGAGTTGAACTGCAAGCTCCGAGGATTCAGCTGGTGAGTATGGCATGATCTTGGTCATATGGGTTGTGTG CAGGTGGAGTTGAACTGCAAGCTCTGAGGATTCAGCTGGTGAGATTGGCATGATCTTGGTCATGTGGGTTGTGTT : : : : : : : : : : : : : : : : 299 299 298 298 298 300 299 300 300 300 300 298 298 298 298 298 FspNB-VN FspCB-VN FgigSN-AY2 FgigTH-HM0 FgigKE-EU0 FgET FgEQB : : : : : : : * 320 * 340 * 360 * TGCTCGGGTCTGCTTAGCTGCAGGTCTCCGCTTCGGTGGGGATGCGCGAATCACTCATCAGGTGTTGTGCGCCCG GCTCGGGTCTGCTTAGCTGCAGGTCCCCGCTTCGGTGGGGATGCGCGAATCACTCATCAGGTGTTGTGCGCCCGG CTCGGGTCTGCTTAGCTGCAGGTCTCCGCTTCGGTGGGGATGCGCGAATCACTCATCAGGTGTTGTGCGCCCGGG CTCGGGTCTGCTTAGCTGCAGGTCTCCGCTTCGGTGGGGATGCGCGAATCACTCATCAGGTGTTGTGCGCCCGGG CTCGGGTCTGCTTAGCTGCAGGTCTCCGCTTCGGTGGGGATGCGCGAATCACTCATCAGGTGTTGTGCGCCCGGG GCTCGGGTCTGCTTAGCTGCAGGTCTCCGCTTCGGTGGGGATGCGCGAATCACTCATCAGGTGTTGTGCGCCCGG GCTCGGGTCTGCTTAGCTGCAGGTCCCCGCTTCGGTGGGGATGCGCGAATCACTCATCAGGTGTTGTGCGCCCGG : : : : : : : 374 374 373 373 373 375 374 62 FhEQB FspEQO FhAus FhBe FhepEG-EU0 FhepSA-AY2 FjacLK-EU0 FmagUS-EU0 FbusVN-EU0 : : : : : : : : : GTGCTCGGGTCTGCTTAGCTGCAGGTCCCCGCTTCGGTGGGGATGCGCGAATCACTCATCAGGTGTTGTGCGCCC TGCTCGGGTCTGCTTAGCTGCAGGTCCCCGCTTCGGTGGGGATGCGCGAATCACTCATCAGGTGTTGTGCGCCCG GTGCTCGGGTCTGCTTAGCTGCAGGTCCCCGCTTCGGTGGGGATGCGCGAATCACTCATCAGGTGTTGTGCGCCC TGTGCTCGGGTCTGCTTAGCTGCAGGTCCCCGCTTCGGTGGGGATGCGCGAATCACTCATCAGGTGTTGTGCGCC CTCGGGTCTGCTTAGCTGCAGGTCCCCGCTTCGGTGGGGATGCGCGAATCACTCATCAGGTGTTGTGCGCCCGGG CTCGGGTCTGCTTAGCTGCAGGTCCCCGCTTCGGTGGGGATGCGCGAATCACTCATCAGGTGTTGTGCGCCCGGG CTCGGGTCTGCTTAGCTGCAGGTCCCCGCTTCGGCGGGGGTGCGCGTATCACTCATCAGGTGTTGTGCGCCCGGG CTCGGGTCTGCTTAGCTGCAGGTCCCCGCTTCGGCGGGGGTGCGCGTATCACTCATCAGGTGTTGTGCGCCCGGG ATCGGGTCTGCTTAGCTGCAGGTCCCCGCTTCGGCGGGGGTGCGCGAATCACTTATCAAGTGTTGTGCGCCCGGT : : : : : : : : : 375 375 375 375 373 373 373 373 373 FspNB-VN FspCB-VN FgigSN-AY2 FgigTH-HM0 FgigKE-EU0 FgET FgEQB FhEQB FspEQO FhAus FhBe FhepEG-EU0 FhepSA-AY2 FjacLK-EU0 FmagUS-EU0 FbusVN-EU0 : : : : : : : : : : : : : : : : 380 * 400 * 420 * 440 * GGTACTGACGGACCTGCTTGCCAGTGCACTTTCTCAGAGTGTTCACCACGACCGACGTTGCTGTCTGGCTGTTGC GTACTGACGGACCTGCTTGCCAGTGCACTTTCTCAGAGTGTTCACCACGACCGACGTTGCTGTCTGGCTGTTGCG TACTGACGGACCTGCTTGCCAGTGCACTTTCTCAGAGTGTTCACCACGACCGACGTTGCTGTCTGGCTGTTGCGG TACTGACGGACCTGCTTGCCAGTGCACTTTCTCAGAGTGTTCACCACGACCGACGTTGCTGTCTGGCTGTTGCGG TACTGACGGACCTGCTTGCCAGTGCACTTTCTCAGAGTGTTCACCACGACCGACGTTGCTGTCTGGCTGTTGCGG GTACTGACGGACCTGCTTGCCAGTGCACTTTCTCAGAGTGTTCACCACGACCGACGTTGCTGTCTGGCTGTTGCG GTACTGACGGACCTGCTTGCCAGTGCACTTTCTCAGAGTGTTCACCACGACCGACGTTGCTGTCTGGCTGTTGCG GGGTACTGACGGACCTGCTTGCCAGTGCACTTTCTCAGAGTGTTCACCACGACCGACGTTGCTGTCTGGCTGTTG GGTACTGACGGACCTGCTTGCCAGTGCACTTTCTCAGAGTGTTCACCACGACCGACGTTGCTGTCTGGCTGTTGC GGGTACTGACGGACCTGCTTGCCAGTGCACTTTCTCAGAGTGTTCACCACGACCGACGTTGCTGTCTGGCTGTTG CGGGTACTGACGGACCTGCTTGCCAGTGCACTTTCTCAGAGTGTTCACCACGACCGACGTTGCTGTCTGGCTGTT TACTGACGGACCTGCTTGCCAGTGCACTTTCTCAGAGTGTTCACCACGACCGACGTTGCTGTCTGGCTGTTGCGG TACTGACGGACCTGCTTGCCAGTGCACTTTCTCAGAGTGTTCACCACGACCGACGTTGCTGTCTGGCTGTTGCGG TATTGACGGACCTGCTTGCCAGTGCACTTTCTCAGAGTGTTCACCACGACCGGCGTTGCCGTCTGGCTGTTGCGG TGTTGACGGACCTGCTTGCCAGTGCACTTTCTCGGAGTGTTCACCACGACCGGCGTTGCTGTCTGACTGTTGCGG TAATGACGAGCCCGCTCGCCAGTGCACTTTCTCAGAGTGTTCACCACGACCGGCGTTGCCGTCTGGCTGTTGCGG : : : : : : : : : : : : : : : : 449 449 448 448 448 450 449 450 450 450 450 448 448 448 448 448 FspNB-VN FspCB-VN FgigSN-AY2 FgigTH-HM0 FgigKE-EU0 FgET FgEQB FhEQB FspEQO FhAus FhBe FhepEG-EU0 FhepSA-AY2 FjacLK-EU0 FmagUS-EU0 FbusVN-EU0 : : : : : : : : : : : : : : : : 460 * 480 * 500 * 520 GGTTAAACCGGCTTGGTAGGGTCCTTGTGTCCTTGCTAGGTCGGGATGGCAGGTAGCCCGTTGTGCGATTTATCG GTTAAACCGGCTTGGTAGGGTCCTTGTGTCCTTGCTAGGTCGGGATGGCAGGTAGCCCGTTGTGCGATTTATCGT TTAAACCGGCTTGGTAGGGTCCTTGTGTCCTTGCTAGGTCGGGATGGCAGGTAGCCCGTTGTGCGATTTATCGTG TTAAACCGGCTTGGTAGGGTCCTTGTGTCCTTGCTAGGTCGGGATGGCAGGTAGCCCGTTGTGCGATTTATCGTG TTAAACCGGCTTGGTAGGGTCCTTGTGTCCTTGCTAGGTCGGGATGGCAGGTAGCCCGTTGTGCGATTTATCGTG GTTAAACCGGCTTGGTAGGGTCCTTGTGTCCTTGCTAGGTCGGGATGGCAGGTAGCCCGTTGTGCGATTTATCGT GTTAAACCGGCTTGGTAGGGTCCTTGTGTCCTTGCTAGGTCGGGATGGCAGGTAGCCCGTTGTGCGATTTATCGT CGGTTAAACCGGCTTGGTAGGGTCCTTGTGTCCTTGCTAGGTCGGGATGGCAGGTAGCCCGTTGTGCGATTTATC GGTTAAACCGGCTTGGTAGGGTCCTTGTGTCCTTGCTAGGTCGGGATGGCAGGTAGCCCGTTGTGCGATTTATCG CGGTTAAACCGGCTTGGTAGGGTCCTTGTGTCCTTGCTAGGTCGGGATGGCAGGTAGCCCGTTGTGCGATTTATC GCGGTTAAACCGGCTTGGTAGGGTCCTTGTGTCCTTGCTAGGTCGGGATGGCAGGTAACCCGTTGTGCGATTTAT TTAAACCGGCTTGGTAGGGTCCTTGTGTCCTTGCTAGGTCGGGATGGCAGGTAGCCCGTTGTGCGATTTATCGTG TTAAACCGGCTTGGTAGGGTCCTTGTGTCCTTGCTAGGTCGGGATGGCAGGTAGCCCGTTGTGCGATTTATCGTG TTAAACCGGCCTGGTAGGGTCCTTGTGGCCTTGCTAGGTCGGGATGGCAGGTAGCCCGTTGTGCGATTTATTGTG TTAAACCGGTTTGGTAGGGTCCTTGTGGCCTTGCTAGGTCGGGATGGCAGGTAGCCCGTTGTGCGATTTATCGTG TTAAACCGGCCTGGTAGGGTCCTTGTGGCTCTGCTAGGTCGGGATGGCAGGTAGCCTGTTGTACAATTTATTGTG : : : : : : : : : : : : : : : : 524 524 523 523 523 525 524 525 525 525 525 523 523 523 523 523 FspNB-VN FspCB-VN FgigSN-AY2 FgigTH-HM0 FgigKE-EU0 FgET FgEQB FhEQB FspEQO FhAus FhBe FhepEG-EU0 FhepSA-AY2 FjacLK-EU0 FmagUS-EU0 FbusVN-EU0 : : : : : : : : : : : : : : : : * 540 * 560 * 580 * 600 TGCTTTGGGTGTAATAGCCGACTGCTTCGGTTCTGTGCGGTACGTCGGAGACGGCGGCTTGTTGTGCGTGCGGGC GCTTCGGGTGTAATAGCCGACTGCTTCGGTTCTGTGCGGTACGTCGGAGACGGCGGCTTGTTGTGCGTGCGGGCG CTTTGGGTGTAATAGCCGACTGCTTCGGTTCTGTGCGGTACGTCGGAGACGGCGGCTTGTTGTGCGTGCGGGCGT CTTCGGGTGTAATAGCCGACTGCTTCGGTTCTGTGCGGTACGTCGGAGACGGCGGCTTGTTGTGCGTGCGGGCGT CTTTGGGTGTAATAGCCGACTGCTTCGGTTCTGTGCGGTACGTCGGAGACGGCGGCTTGTTGTGCGTGCGGGCGT GCTTTGGGTGTAATAGCCGACTGCTTCGGTTCTGTGCGGTACGTCGGAGACGGCGGCTTGTTGTGCGTGCGGGCG GCTTCGGGTGTAATAGCCGACTGCTTCGGTTCTGTGCGGTACGTCGGAGACGGCGGCTTGTTGTGCGTGCGGGCG GTGCTTCGGGTGTAATAGCCGACTGCTTCGGTTCTGTGCGGTACGTCGGAGACGGCGGCTTGTTGTGCGTGCGGG TGCTTCGGGTGTAATAGCCGACTGCTTCGGTTCTGTGCGGTACGTCGGAGACGGCGGCTTGTTGTGCGTGCGGGC GTGCTTCGGGTGTAATAGCCGACTGCTTCGGTTCTGTGCGGTACGTCGGAGACGGCGGCTTGTTGTGCGTGCGGG CGTGCTTCGGGTGTAATAGCCGACTGCTTCGGTTCTGTGCGGTACGTCGGAGACGGCGGCTTGTTGTGCGTGCGG CTTCGGGTGTAATAGCCGACTGCTTCGGTTCTGTGCGGTACGTCGGAGACGGCGGCTTGTTGTGCGTGCGGGCGT CTTCGGGTGTAATAGCCGACTGCTTCGGTTCTGTGCGGTACGTCGGAGACGGCGGCTTGTTGTGCGTGCGGGCGT CTTCGGGTGTAATAGCCGACTGCTTCGGTTCTGTGCGGTACGCCGGAGACGGCGGCTTGTTGTGCTTGCGGGCGT CTTCGGGTGTAATAGCCGACTGCTTCGGTTCTGTGCGGTACGTCGGAGACGGCGGCTTGTTGTGCGTGCGGGCGT CTCCGGGTGTAATAGCCGACTGCTTCGGTTCTGTGCGGTGCGTCGGAGACGGCGGCTTGTTGTGCTTGCAGGCGT : : : : : : : : : : : : : : : : 599 599 598 598 598 600 599 600 600 600 600 598 598 598 598 598 FspNB-VN FspCB-VN FgigSN-AY2 FgigTH-HM0 FgigKE-EU0 FgET FgEQB FhEQB FspEQO FhAus FhBe FhepEG-EU0 FhepSA-AY2 FjacLK-EU0 FmagUS-EU0 FbusVN-EU0 : : : : : : : : : : : : : : : : * 620 * 640 * 660 * GTGCCTGTCGCGCCGGCGGCTCTGGGTCTGGTTGCCTTGTTGCTTGTGAATGCAGGCCAGGTGATGGCTCGGTGT TGCCTGTCGCGCCGGCGGCTCTGGGTCTGGTTGCCTTGTTGCTTGTGAATGCAGGCCAGGTGATGGCTCGGTGTC GCCTGTCGCGCCGGCGGCTCTGGGTCTGGTTGCCTTGTTGCTTGT-AATGCAGGCCAGGTGATGGCTCGGTGTCG GCCTGTCGCGCCGGCGGCTCTGGGTCTGGTTGCCTTGTTGCTTGTGAATGCAGGCCAGGTGATGGCTCGGTGTCG GCCTGTCGCGCCGGCGGCTCTGGGTCTGGTTGCCTTGTTGCTTGTGAATGCAGGCCAGGTGATGGCTCGGTGTCG TGCCTGTCGCGCCGGCGGCTCTGGGTCTGGTTGCCTTGTTGCTTGTGAATGCAGGCCAGGTGATGGCTCGGTGTC TGCCTGTCGCGCCGGCGGCTCTGGGTCTGGTTGCCTTGTTGCTTGTGAATGCAGGCCAGGTGATGGCTCGGTGTC CGTGCCTGTCGCGCCGGCGGCTCTGGGTCTGGTTGCCTTGTTGCTTGTGAATGCAGGCCAGGTGATGGCTCGGTG GTGCCTGTCGCGCCGGCGGCTCTGGGTCTGGTTGCCTTGTTGCTTGTGAATGCAGGCCAGGTGATGGCTCGGTGT CGTGCCTGTCGCGCCGGCGGCTCTGGGTCTGGTTGCCTTGTTGCTTGTGAATGCAGGCCAGGTGATGGCTCGGTG GCGTGCCTGTCGCGCCGGCGGCTCTGGGTCTGGTTGCCTTGTTGCTTGTGAATGCAGGCCAGGTGATGGCTCGGT GCCTGTCGCGCCGGCGGCTCTGGGTCTGGTTGCCTTGTTGCTTGTGAATGCAGGCCAGGTGATGGCTCGGTGTCG GCCTGTCGCGCCGGCGGCTCTGGGTCTGGTTGCCTTGTTGCTTGTGAATGCAGGCCAGGTGATGGCTCGGTGTCG GCCTGTCGCGCCGGCGGCTCTGGGTCTGGTTGCCTTGTTGCTTGTGAATGCAGGCCAGGTGATGGCTCGGTGTCG GCCTGTCGCGTCGGCGGCTCTGGGTCTGGTTGCCTTGTTGCTTGTGAATGCAGGCCAGGTGATGGCTCGGTGTCG GCCTGCTGTGCTGGCGGCTCTGAGTCTGGTTGCCTTGTTGCTTGTGAATACAAGCCAGGTGATGGCTTGGTGTCG : : : : : : : : : : : : : : : : 674 674 672 673 673 675 674 675 675 675 675 673 673 673 673 673 FspNB-VN FspCB-VN FgigSN-AY2 FgigTH-HM0 FgigKE-EU0 : : : : : 680 * 700 * 720 * 740 * CGTTCGGTCGGCGGTTGCGTTTGTTGCACTTTGAAAGGGCCCACAGTCTGTGGTGTAGTGGTAGACTATCCACCT GTTCGGTCGGCGGTTGCGTTTGTTGCACTTTGAAAGGGCCCACAGTCTGTGGTGTAGTGGTAGACTATCCACCTG TTCGGTCGGCGGTTGCGTTTGTTGCACTTTGAAAGGGCCCACAGTCTGTGGTGTAGTGGTAGACTATCCACCTGA TTCGGTCGGCGGTTGCGTTTGTTGCACTTTGAAAGGGCCCACAGTCTGTGGTGTAGTGGTAGACTATCCACCTGA TTCGGTCGGCGGTTGCGTTTGTTGCACTTTGAAAGGGCCCACAGTCTGTGGTGTAGTGGTAGACTATCCACCTGA : : : : : 749 749 747 748 748 63 FgET FgEQB FhEQB FspEQO FhAus FhBe FhepEG-EU0 FhepSA-AY2 FjacLK-EU0 FmagUS-EU0 FbusVN-EU0 : : : : : : : : : : : GTTCGGTCGGCGGTTGCGTTTGTTGCACTTTGAAAGGGCCCACAGTCTGTGGTGTAGTGGTAGACTATCCACCTG GTTCGGTCGGCGGTTGCGTTTGTTGCACTTTGAAAGGGCCCACAGTCTGTGGTGTAGTGGTAGACTATCCACCTG TCGTTCGGTCGGCGGTTGCGTTTGTTGCACTTTGAAAGGGCCCACAGTCTGTGGTGTAGTGGTAGACTATCCACC CGTTCGGTCGGCGGTTGCGTTTGTTGCACTTTGAAAGGGCCCACAGTCTGTGGTGTAGTGGTAGACTATCCACCT TCGTTCGGTCGGCGGTTGCGTTTGTTGCACTTTGAAAGGGCCCACAGTCTGTGGTGTAGTGGTAGACTATCCACC GTCGTTCGGTCGGCGGTTGCGTTTGTTGCACTTTGAAAGGGCCCACAGTCTGTGGTGTAGTGGTAGACTATCCAC TTCGGTCGGCGGTTGCGTTTGTTGCACTTTGAAAGGGCCCACAGTCTGTGGTGTAGTGGTAGACTATCCACCTGA TTCGGTCGGCGGTTGCGTTTGTTGCACTTTGAAAGGGCCCACAGTCTGTGGTGTAGTGGTAGACTATCCACCTGA TTCGGTCGGCGGTTGCGTTTGTTGCACTTTGAAAGGGCCCACAGTCTGTGGTGTAGTGGTAGACTATCCACCTGA TTCGGCCGGCGGTTGCGTCTGTTGCACTTTGAWAGGGCCCACAGTCTGTGGTGTAGTGGTAGACTATCCACCTGA TTTGGTCGGCGGTTGCGTTTGTTGCACTTTAAAAGGGCCCATAGTCTGTGGTGTAGTGGTAGACTATCCACCTGA : : : : : : : : : : : 750 749 750 750 750 750 748 748 748 748 748 FspNB-VN FspCB-VN FgigSN-AY2 FgigTH-HM0 FgigKE-EU0 FgET FgEQB FhEQB FspEQO FhAus FhBe FhepEG-EU0 FhepSA-AY2 FjacLK-EU0 FmagUS-EU0 FbusVN-EU0 : : : : : : : : : : : : : : : : 760 * 780 * 800 * 820 GACCCGTCTTGAAACACGGACCAAGGAGAGTAACATGTGCGCGAGTCATTGGGCGTTACGAAACCCAAAGGCGCA ACCCGTCTTGAAACACGGACCAAGGAGAGTAACATGTGCGCGAGTCATTGGGCGTTACGAAACCCAAAGGCGCAG CCCGTCTTGAAACACGGACCAAGGAGAGTAAACTGTGCGCGAGTCATTGGGCGTTACGAAACCCAAAGGCGCAGT CCCGTCTTGAAACACGGACCAAGGAGAGTAACATGTGCGCGAGTCATTGGGCGTTACGAAACCCAAAGGCGCAGT CCCGTCTTGAAACACGGACCAAGGAGAGTAACATGTGCGCGAGTCATTGGGCGTTACGAAACCCAAAGGCGCAGT ACCCGTCTTGAAACACGGACCAAGGAGAGTAACATGTGCGCGAGTCATTGGGCGTTACGAAACCCAAAGGCGCAG ACCCGTCTTGAAACACGGACCAAGGAGAGTAACATGTGCGCGAGTCATTGGGCGTTACGAAACCCAAAGGCGCAG TGACCCGTCTTGAAACACGGACCAAGGAGAGTAACATGTGCGCGAGTCATTGGGCGTTACGAAACCCAAAGGCGC GACCCGTCTTGAAACACGGACCAAGGAGAGTAACATGTGCGCGAGTCATTGGGCGTTACGAAACCCAAAGGCGCA TGACCCGTCTTGAAACACGGACCAAGGAGAGTAACATGTGCGCGAGTCATTGGGCGTTACGAAACCCAAAGGCGC CTGACCCGTCTTGAAACACGGACCAAGGAGAGTAACATGTGCGCGAGTCATTGGGCGTTACGAAACCCAAAGGCG CCCGTCTTGAAACACGGACCAAGGAGAGTAACATGTGCGCGAGTCATTGGGCGTTACGAAACCCAAAGGCGCAGT CCCGTCTTGAAACACGGACCAAGGAGAGTAACATGTGCGCGAGTCATTGGGCGTTACGAAACCCAAAGGCGCAGT CCCGTCTTGAAACACGGACCAAGGAGAGTAACATGTGCGCGAGTCATTGGGCGTTACGAAACCCAAAGGCGCAGT CCCGTCNNGAAACWCGGACCAAGGAGAGTAACATGTGCGCGAGTCATTGGGCGTTACGAAACCCAAAGGCGAAGT CCCGTCTTGAAACACGGACCAAGGAGAGTAACATGTGCGCGAGTCATTGGGCGTTACGAAACCCAAAGGCGCAGT : : : : : : : : : : : : : : : : 824 824 822 823 823 825 824 825 825 825 825 823 823 823 823 823 FspNB-VN FspCB-VN FgigSN-AY2 FgigTH-HM0 FgigKE-EU0 FgET FgEQB FhEQB FspEQO FhAus FhBe FhepEG-EU0 FhepSA-AY2 FjacLK-EU0 FmagUS-EU0 FbusVN-EU0 : : : : : : : : : : : : : : : : * 840 * 860 * 880 * 900 GTGAAAGTGAAGGCTCGGCTTGTCTGGGCTGAGGTGAGATCCTGTCGTTTCTCATGCGCGGTACTACCAAGCTTC TGAAAGTGAAGGCTCGGCTTGTCTGGGCTGAGGTGAGATCCTGTCGTTTCTCATGCGCGGTACTACCAAGCTTCG GAAAGTGAAGGCTCGGCTTGTCTGGGCTGAGGTGAGATCCTGTCGTTTCTCATGCGCGGTACTACCAAGCTTCGA GAAAGTGAAGGCTCGGCTTGTCTGGGCTGAGGTGAGATCCTGTCGTTTCTCATGCGCGGTACTACCAAGCTTCGA GAAAGTGAAGGCTCGGCTTGTCTGGGCTGAGGTGAGATCCTGTCGTTTCTCATGCGCGGTACTACCAAGCTTCGA TGAAAGTGAAGGCTCGGCTTGTCTGGGCTGAGGTGAGATCCTGTCGTTTCTCATGCGCGGTACTACCAAGCTTCG TGAAAGTGAAGGCTCGGCTTGTCTGGGCTGAGGTGAGATCCTGTCGTTTCTCATGCGCGGTACTACCAAGCTTCG AGTGAAAGTGAAGGCTCGGCTTGTCTGGGCTGAGGTGAGATCCTGTCGTTTCTCATGCGCGGTACTACCAAGCTT GTGAAAGTGAAGGCTCGGCTTGTCTGGGCTGAGGTGAGATCCTGTCGTTTCTCATGCGCGGTACTACCAAGCTTC AGTGAAAGTGAAGGCTCGGCTTGTCTGGGCTGAGGTGAGATCCTGTCGTTTCTCATGCGCGGTACTACCAAGCTT CAGTGAAAGTGAAGGCTCGGCTTGTCTGGGCTGAGGTGAGATCCTGTCGTTTCTCATGCGCGGTACTACCAAGCT GAAAGTGAAGGCTCGGCTTGTCTGGGCTGAGGTGAGATCCTGTCGTTTCTCATGCGCGGTACTACCAAGCTTCGA GAAAGTGAAGGCTCGGCTTGTCTGGGCTGAGGTGAGATCCTGTCGTTTCTCATGCGCGGTACTACCAAGCTTCGA GAAAGTAAAGGCTTGGCTTGTCTGAGCTGAGGTGAGATCCTGTCGTTTCTCATGCGCGGTACCACCAAGCTTCGA GAAAGTAAAGGCTCGGCTTGTYTGAGCTGAGGWGAGATCCTGTCSTTTCTCATGCCCGGTACTACCAAGCTTCGA GAAAGTAAAGGCTTGGCTTGTCTGAGCTGAGGTGAGATCCTGTCGTTTCTCATGCGCGGTACTACCAAGCTTCGA : : : : : : : : : : : : : : : : 899 899 897 898 898 900 899 900 900 900 900 898 898 898 898 898 FspNB-VN FspCB-VN FgigSN-AY2 FgigTH-HM0 FgigKE-EU0 FgET FgEQB FhEQB FspEQO FhAus FhBe FhepEG-EU0 FhepSA-AY2 FjacLK-EU0 FmagUS-EU0 FbusVN-EU0 : : : : : : : : : : : : : : : : * 920 * 940 * 960 * GAGCGGCAGGCGCATCACCGGCCCGTCCCATGGCGTAGAGGCAGCCTCGTGCTTGCTCATCGTCGGGGCGGAGCA AGCGGCAGGCGCATCACCGGCCCGTCCCATGGCGTAGAGGCAGCCTCGTGCTTGCTCATCGTCGGGGCGGAGCAT GCGGCAGGCGCATCACCGGCCCGTCCCATGGCGTAGAGGCAGCCTCGTGCTTGCTCATCGTCGGGGCGGAGCATG GCGGCAGGCGCATCACCGGCCCGTCCCATGGCGTAGAGGCAGCCTCGTGCTTGCTCATCGTCGGGGCGGAGCATG GCGGCAGGCGCATCACCGGCCCGTCCCATGGCGTAGAGGCAGCCTCGTGCTTGCTCATCGTCGGGGCGGAGCATG AGCGGCAGGCGCATCACCGGCCCGTCCCATGGCGTAGAGGCAGCCTCGTGCTTGCTCATCGTCGGGGCGGAGCAT AGCGGCAGGCGCATCACCGGCCCGTCCCATGGCGTAGAGGCAGCCTCGTGCTTGCTCATCGTCGGGGCGGAGCAT CGAGCGGCAGGCGCATCACCGGCCCGTCCCATGGCGTAGAGGCAGCCTCGTGCTTGCTCATCGTCGGGGCGGAGC GAGCGGCAGGCGCATCACCGGCCCGTCCCATGGCGTAGAGGCAGCCTCGTGCTTGCTCATCGTCGGGGCGGAGCA CGAGCGGCAGGCGCATCACCGGCCCGTCCCATGGCGTAGAGGCAGCCTCGTGCTTGCTCATCGTCGGGGCGGAGC TCGAGCGGCAGGCGCATCACCGGCCCGTCCCATGGCGTAGAGGCAGCCTCGTGCTTGCTCATCGTCGGGGCGGAG GCGGCAGGCGCATCACCGGCCCGTCCCATGGCGTAGAGGCAGCCTCGTGCTTGCTCATCGTCGGGGCGGAGCATG GCGGCAGGCGCATCACCGGCCCGTCCCATGGCGTAGAGGCAGCCTCGTGCTTGCTCATCGTCGGGGCGGAGCATG GCGGCAGGCGCATCACCGGCCCGTCCCATGGCGTAGAGGCGGCCTCGAGCTTGCTCATCGTCGGGGCGGAGCATG GCGGCAGGCGCATCACCGGCCCGTCCCATGGCGTAGAGGCAGCCTCGTGCTTGCTCATCGTCGGGGCGGAGCATG GCGGCAGGCGCATCACCGGCCCGTCCCATGGCGTGGAGGCAGCCTCGTGCTTGCTCATCGTCGGGGCGGAGCATG : : : : : : : : : : : : : : : : 974 974 972 973 973 975 974 975 975 975 975 973 973 973 973 973 FspNB-VN FspCB-VN FgigSN-AY2 FgigTH-HM0 FgigKE-EU0 FgET FgEQB FhEQB FspEQO FhAus FhBe FhepEG-EU0 FhepSA-AY2 FjacLK-EU0 FmagUS-EU0 FbusVN-EU0 : : : : : : : : : : : : : : : : 980 * 1000 * 1020 * 1040 TGAGCGTACATGTTGAGACCCGAAAGATGGTGAACTATGCTTGCGCAGGTTGAAGCCAGAGGAAA-GAGCGTACATGTTGAGACCCGAAAGATGGTGAACTATGCTTGCGCAGGTTGAAGCCAGAGGAAA AGCGTACATGTTGAGACCCGAAAGATGGTGAACTATGCTTGCGCAGGTTGAAGCCAGAGGAAA -AGCGTACATGTTGAGACCCGAAAGATGGTGAACTATGCTTGCGCAGGTTGAAGCCAGAGGAAA -AGCGTACATGTTGAGACCCGAAAGATGGTGAACTATGCTTGCGCAGGTTGAAGCCAGAGGAAA -GAGCGTACATGTTGAGACCCGAAAGATGGTGAACTATGCTTGCGCAGGTTGAAGCCAGAGGAAA GAGCGTACATGTTGAGACCCGAAAGATGGTGAACTATGCTTGCGCAGGTTGAAGCCAGAGGAAA ATGAGCGTACATGTTGAGACCCGAAAGATGGTGAACTATGCTTGCGCAGGTTGAAGCCAGAGGAAATGAGCGTACATGTTGAGACCCGAAAGATGGTGAACTATGCTTGCGCAGGTTGAAGCCAGAGGAAA-ATGAGCGTACATGTTGAGACCCGAAAGATGGTGAACTATGCTTGCGCAGGTTGAAGCCAGAGGAAACATGAGCGTACATGTTGAGACCCGAAAGATGGTGAACTATGCTTGCGCAGGTTGAAGCCAGAGGAAA AGCGTACATGTTGAGACCCGAAAGATGGTGAACTATGCTTGCGCAGGTTGAAGCCAGAGGAAA -AGCGTACATGTTGAGACCCGAAAGATGGTGAACTATGCTTGCGCAGGTTGAAGCCAGAGGAAA -AGCGTACATGTTGAGACCCGAAAGATGGTGAACTATGCTTGCGCAGGTTGAAGCCAGAGGAAA -AGCGTACATGTTGAGACCCGAAAGATGGTGAACTATGCTTGCGCAGGTTGAAGCCAGAGGAAA -AGCGTACATGTTGAGACCCGAAAGATGGTGAACTATGCTTGCGCAGGTTGAAGCCAGAGGAAA : : : : : : : : : : : : : : : : 1039 1038 1035 1036 1036 1039 1038 1041 1040 1041 1042 1036 1036 1036 1036 1036 64 Phụ lục Giản đồ giải trình tự nucleotide gan 28S FspCB-VN FspNB-VN 65 Phụ lục Trình tự nucleotide gen cox1 FspNB-VN-c FspCB-VN-c Fhep-AU-co FspGHL-CNFgig-CN-co Fgig-ID-co Fbus(human Fbus(swine Csin-CN-co Csin-KR-co Csin-RU-co Csin-VN-co Oviv-TH-co Oviv-CN-co Ofel-RU-co Phet-VN-co Pohi-JP-co Pwes(2n)-K Pwes(3n)KR Ejap-VN-co Htai-KR-co Htai-LA-co Htai-VN-co Sfal-VN-co : : : : : : : : : : : : : : : : : : : : : : : : * 20 * 40 * 60 * GTGAATTATATTAGTTGATTGTTTACGTTAGATCATAAGCGTGTTGGTTTGATTTATATGTTGATTGGTCTTTGG GTGAATTATATTAGTTGATTGTTAACGTTAGATCATAAGCGTGTTGGTTTGATTTATATGTTGATTGGTCTTTGG GTGAAGTATATGAGTTGGTTGTTTACGTTGGATCATAAGCGTGTTGGTTTGATTTATATGGTTATTGGTCTTTGG GTGAATTATATTAGTTGATTGTTTACGTTAGATCATAAGCGTGTTGGTTTGATTTATATGTTGATTGGTCTTTGG GTGAATTATATTAGTTGATTGTTTACGTTAGATCATAAGCGTGTTGGTTTGATTTATATGTTGATTGGTCTTTGG GTGAATTATATTAGTTGGTTGTTTACGTTAGATCATAAGCGTGTTGGTTTGATTTATATGTTGATTGGTCTTTGG GTGAGATATTTTGGTTGGTTGTTTACTTTAGATCATAAGCGTATTGGTTTTGTTTATTTGGTAGTTGGTTTGTGG GTGAGATATTTTGGTTGGTTGTTTACTTTAGATCATAAGCGTATTGGTTTTGTTTATTTGGTAGTTGGTTTGTGG GTGTCTGCGATATGGCTCCTTACTATGGATCATAAGCGTATAGGTGTTATTTATATGATTCTTGGGATCTGGGGT GTGTCTGCGATATGGCTCCTTACTATGGATCATAAGCGTATAGGTGTTATTTATATGATTCTTGGGATCTGGGGT GTGTCTGCGATATGGCTCCTTACTATGGATCATAAGCGTATAGGTGTTATTTATATGATTCTTGGTATCTGGGGT GTGTCTGCGATATGGCTCCTTACTATGGATCATAAGCGTATAGGTGTTATTTATATGATTCTTGGGATCTGGGGT GTGTCTACGATATGACTTCTTACAATGGATCATAAGCGTATTGGTGTTATTTACATGATTTTGGGGGTCTGAGGT GTGTCTACGATATGGCTTCTTACAATGGATCATAAGCGTATTGGTGTTATTTACATGATTTTGGGGGTCTGAGGT GTGTCTGCGATATGGCTTTTTACTATGGATCATAAGCGTATTGGTATTATTTATATGATTTTGGGTGTGTGAGGC ATGTTTACTTGGTTGTTTACTTTAGATCATAAGCGTATAGGTGTGATTTACATGGTCTTGGGTGTGTGGTGTGGA -ATGACTTGATTATTTACTTTGGATCATAAGCGTATTGGTATGATTTACATGGTCTTGGGTGTGTGAGGTGGA ATGTTTACTTGGTTGTTCACTTTGGATCACAAGCGTATCGGGATGATTTACATGGTTTTGGGGATTTGGGGAGGA ATGTTTACTTGGTTGTTCACTTTGGATCACAAGCGTATCGGGATGATTTACATGGTTTTGGGGATTTGGGGAGGA ATGATGTGGATGACTTGGTTATTTACTTTAGATCATAAGCGAATTGGTTTAATTTATATGTTGATTGGGGTATGA ATGTTGACTTGATTATTGACTTTGGATCATAAGCGAATAGGGTTGATATATATGATTGTTGGAGTGTGAGGGGGC ATGTTGACTTGATTATTGACTTTGGATCATAAGCGAATAGGGTTGATATATATGATTGTTGGAGTGTGAGGGGGC ATGTTGACTTGATTATTGACTTTGGATCATAAGCGAATAGGGTTGATATATATGATTGTTGGAGTGTGAGGGGGC GTGTTGCTCTGGTTTTTTACGCTGGATCATAAGCGTGTTGGTATGATTTATTTTCTTTTGGGTGTATGGGGAGGC : : : : : : : : : : : : : : : : : : : : : : : : 75 75 75 75 75 75 75 75 75 75 75 75 75 75 75 75 72 75 75 75 75 75 75 75 FspNB-VN-c FspCB-VN-c Fhep-AU-co FspGHL-CNFgig-CN-co Fgig-ID-co Fbus(human Fbus(swine Csin-CN-co Csin-KR-co Csin-RU-co Csin-VN-co Oviv-TH-co Oviv-CN-co Ofel-RU-co Phet-VN-co Pohi-JP-co Pwes(2n)-K Pwes(3n)KR Ejap-VN-co Htai-KR-co Htai-LA-co Htai-VN-co Sfal-VN-co : : : : : : : : : : : : : : : : : : : : : : : : 80 * 100 * 120 * 140 * GGGGGATTTTTTGGTCTTTCTTTGAGTGTTTTGGTTCGTTTGAATTATTTGGATCCTTATTTTAATTTGGTGTCC GGTGGATTTTTTGGTCTTTCTTTGAGTATTTTGGTTCGTTTGAATTATTTGGATCCTTATTTTAATTTGGTGTCC GGGGGGTTTTTTGGTCTTTCTTTGAGGGTGTTGGTTCGTTTGAATTATTTAGATCCATATTTTAATTTGGTTTCT GGTGGATTTTTTGGTCTTTCTTTGAGTATTTTGGTTCGTTTGAATTATTTGGATCCTTATTTTAATTTGGTGTCC GGTGGATTTTTTGGTCTTTCTTTGAGTATTTTGGTTCGTTTGAATTATTTGGATCCTTATTTTAATTTGGTGTCT GGGGGATTTTTTGGTCTTTCTTTGAGTGTTTTGGTTCGTTTGAATTATTTGGATCCTTATTTTAATTTGGTGTCC AGAGGGTTTTTGGGTTTGTCGTTGAGTATGTTGATTCGTTTAAAATATTTGGATCCTTATTATAATTTGATTTCT AGAGGGTTTTTGGGTTTGTCGTTGAGTATGTTGATTCGTTTAAAATATTTGGATCCTTATTATAATTTGATTTCT GGATTTTTGGGTCTTGGGTTGAGGTTTCTTATACGAGTGAATTATTTTGATCCTTACTTTAATGTTATTTCATCA GGATTTTTGGGTCTTGGGTTGAGGTTTCTTATACGAGTGAATTATTTTGATCCTTACTTTAATGTTATTTCATCA GGATTTTTGGGTCTTGGGTTGAGGTTTCTTATACGAGTGAATTATTTTGATCCTTACTTTAATGTTATTTCATCA GGATTTTTGGGTCTTGGGTTGAGGTTTCTTATACGAGTGAATTATTTTGATCCTTACTTTAATGTTATTTCATCA GGATTTTTGGGCCTGGGGCTTAGGTTTCTTATACGAGTTAATTATTTTGATCCTTATTTTAATATTGTTTCTCCG GGATTTTTGGGCCTGGGGCTTAGGTTTCTTATACGAGTTAATTATTTTGATCCTTATTTTAATATTGTTTCTCCG GGGTTTACAGGATTGAGGTTGAGGTTTCTTATACGGATAAATTATTTTGATCCCTATTTTAATGTTGTTTCTCCC TTTTTGGGGCTCTCTTTAAGGATGTTGATTCGCTTGAACTA-CTTGGATCCTTACTACAATATTGTTTCGTCGGA TTTATTGGACTTTCTTTAAGAATGTTAATTCGTTTGAATTATCTTG-ATCCGTATTATAATATTGTCTCGTCGGA TTTCTGGGTCTTTCTCTGAGGATGTTGATTCGTCTGAACTA-CCTGGATCCTTACTACAACATTGTCTCGTCGGA TTTCTGGGTCTTTCTCTGAGGATGTTGATTCGTCTGAACTA-CCTGGATCCTTACTACAACATTGTCTCGTCGGA GGGGGTTTTTTGGGTCTTTCTTTGAGTTTGTTGATACGTTTGAAATATTTGGATCCTTATTTTAATTTGATAATA TTTTTAGGTCTTTCTTTGAGGTTTTTGATCCGTGTTAACTACTTGGATCCTTACTATAATGTTGTATCCCCAGAG TTTTTAGGTCTTTCTTTGAGGTTTTTGATCCGTGTTAACTACTTGGATCCTTACTATAATGTTGTATCCCCAGAG TTTTTAGGTCTTTCTTTGAGGTTTTTGATCCGTGTTAACTACTTGGATCCTTACTATAATGTTGTATCCCCAGAG TTTTTGGGGCTTTCTTTGAGCTTTCTTATTCGGGTAAAATACTTGGATCCTTATTTTAAAGTCGTTTCGTCGGAT : : : : : : : : : : : : : : : : : : : : : : : : 150 150 150 150 150 150 150 150 150 150 150 150 150 150 150 149 146 149 149 150 150 150 150 150 FspNB-VN-c FspCB-VN-c Fhep-AU-co FspGHL-CNFgig-CN-co Fgig-ID-co Fbus(human Fbus(swine Csin-CN-co Csin-KR-co Csin-RU-co Csin-VN-co Oviv-TH-co Oviv-CN-co Ofel-RU-co Phet-VN-co Pohi-JP-co Pwes(2n)-K Pwes(3n)KR Ejap-VN-co Htai-KR-co Htai-LA-co Htai-VN-co Sfal-VN-co : : : : : : : : : : : : : : : : : : : : : : : : 160 * 180 * 200 * 220 CCTGAGGTTTATAATTATGTTGTGACGGGACATGGGGTTATTATGATTTTTTTCTTTTTGATGCCTGTGTTGATT CCTGAGGTTTATAATTATGTTGTGACGGGACATGGGGTTATTATGATTTTTTTCTTTTTGATGCCTGTGTTGATT CCTGAAGTTTATAATTATGTTGTTACTGGGCATGGGATTATAATGATTTTTTTCTTTTTGATGCCGGTGTTGATT CCTGAGGTTTATAATTATGTTGTGACGGGACATGGGGTTATTATGATTTTTTTCTTTTTGATGCCTGTGTTGATT CCTGAGGTTTATAATTATGTTGTGACGGGGCATGGTGTTATTATGATTTTTTTCTTTTTAATGCCTGTGTTGATT CCTGAGGTTTATAATTATGTTGTGACGGGACATGGGGTTATTATGATTTTTTTCTTTTTGATGCCTGTGTTGATT CCGGAGGTGTATAATTATTTGGTTACAGGTCATGGTATAGTGATGATTTTTTTTTTTCTGATGCCTGTGTTGATA CCGGAGGTGTATAATTATTTGGTTACAGGTCATGGTATAGTGATGATTTTTTTTTTTCTGATGCCTGTGTTGATA GATGTTTATAATTATGTGGTTACTAGGCATGGGGTTGCTATGATTTTTTTCTTCTTGATGCCTGTTCTTGTGGGC GATGTTTATAATTATGTGGTTACTAGGCATGGGGTTGCTATGATTTTTTTCTTCTTGATGCCTGTTCTTGTGGGC GATGTTTATAATTATGTGGTTACTAGGCATGGGGTTGCTATGATTTTTTTCTTCTTGATGCCTGTTCTTGTGGGC GATGTTAATAATTATGTGGTTACTAGGCATGGGGTTGCTATGATTTTTTTCTTCTTGATGCCTGTTCTTGTGGGC GATGTTTATAATTATATTGTTACGAGTCATGGGATAGCTATGATCTTTTTCTTTTTAATGCCTGTTCTTATTGGT GGTGTTTATAATTATATTGTTACGAGTCATGGGATAGCTATGATCTTTTTCTTTTTAATGCCTGTTCTTATTGGT GATGTATATAATTACGTTGCTACTAGTCATGGGGTTGCCATGATTTTTTTTTTCTTAATGCCGGTTTTAATAGGG GGTTTACAATTACGTGATAACGAATCATGGTGTTGCTATGATTTTTTTCTTTTTGATGCCTGTCTTGATAGGCGG GGTTTACAATTATGTGATAACTAATCATGGGGTTGCTATGATTTTCTTTTTTTTGATGCCTGTGTTGATAGGAGG GGTTTACAACTATGTGCTGACCAATCATGGGGTCGCCATGATTTTCTTTTTCCTGATGCCTGTTTTGATAGGGGG GGTTTACAACTATGTGCTGACCAATCATGGGGTCGCCATGATTTTCTTTTTCCTGATGCCTGTTTTGATAGGGGG CCTGAGGTATATAATTATGTGATTACTTGACATGGGATAGTGATGATTTTCTTTTTTTTGATGCCTGTTTTGATA GTTTATAATTATGTAATTACTAGGCATGGTATTGCGATGATTTTTTTTTTCTTAATGCCGGTTTTGATTGGGGGG GTTTATAATTATGTAATTACTAGGCATGGTATTGCGATGATTTTTTTTTTCTTAATGCCGGTTTTGATTGGGGGG GTTTATAATTATGTAATTACTAGGCATGGTATTGCGATGATTTTTTTTTTCTTAATGCCGGTTTTGATTGGGGGG GTTTACAACTACATAATTACAAGGCATGGGATAGCGATGATTTTCTTTTTCTTGATGCCCGTGTTAATTGGTAGG : : : : : : : : : : : : : : : : : : : : : : : : 225 225 225 225 225 225 225 225 225 225 225 225 225 225 225 224 221 224 224 225 225 225 225 225 FspNB-VN-c FspCB-VN-c Fhep-AU-co FspGHL-CN- : : : : * 240 * 260 * 280 * 300 GGGGGGTTTGGTAaTTATTTAATGCCTTTGCTTTTGGGTATTCCTGATTTGAATTTGCCTCGTTTAAATGCTTTG GGGGGGTTTGGTAATTATTTATTGCCTTTGCTTTTGGGTATTCCTGATTTGAATTTACCTCGTTTAAATGCTTTG GGGGGTTTTGGTAATTATTTGTTGCCTTTATTGTTAGGTATTCCTGATTTGAATTTGCCTCGTTTAAATGCTTTG GGGGGGTTTGGTAATTATTTATTGCCTTTGCTTTTGGGTATTCCTGATTTGAATTTACCTCGTTTAAATGCTTTG : : : : 300 300 300 300 66 Fgig-CN-co Fgig-ID-co Fbus(human Fbus(swine Csin-CN-co Csin-KR-co Csin-RU-co Csin-VN-co Oviv-TH-co Oviv-CN-co Ofel-RU-co Phet-VN-co Pohi-JP-co Pwes(2n)-K Pwes(3n)KR Ejap-VN-co Htai-KR-co Htai-LA-co Htai-VN-co Sfal-VN-co : : : : : : : : : : : : : : : : : : : : GGGGGGTTTGGTAATTATTTATTGCCTTTGCTTTTGGGTATTCCTGATTTGAATTTGCCTCGTTTAAATGCTTTG GGGGGGTTTGGTAATTATTTATTGCCTTTGCTTTTGGGTATTCCTGATTTGAATTTGCCTCGTTTAAATGCTTTG GGTGGTTTTGGTAATTATTTGTTACCGTTGTTGTTAGGGATAGGTGATTTGAATTTACCTCGTGTAAATGCTTTG GGTGGTTTTGGTAATTATTTGTTACCGTTGTTGTTAGGGATAGGTGATTTGAATTTACCTCGTGTAAATGCTTTG GGTTTTGGTAACTACCTGCTGCCTCTGTTACTTGGTTTATCAGATTTGAATTTGCCTCGTTTGAAAGCTCTAAGC GGTTTTGGTAACTACCTGCTGCCTCTGTTACTTGGTTTATCAGATTTGAATTTGCCTCGTTTGAAAGCTCTAAGC GGTTTTGGTAACTACCTACTGCCTCTGTTACTTGGTTTATCAGATTTGAATTTGCCTCGTTTGAAAGCTCTAAGC GTTTTTGGTAACTACCTGCTGCCTCTGTTACTTGGTTTATCAGATTTGAATTTGCCTCGTTTGAAAGCTCTAAGT GGGTTTGGTAATTATTTATTGCCCTTGTTGTTGGGTTTATCGGACTTGAATTTGCCCCGTTTAAAAGCGTTGAGT GGGTTTGGTATTTATTTATTGCCCTTGTTGTTGGGTTTATCGGACTTGAATTTGCCCCGTTTAAAAGCGTTGAGT GGTTTTGGTAAATATTTGTTGCCTCTGTTGCTGGGTTTGTCGGATTTAAATTTACCCCGTTTGAAAGCTTTAAGT GTTTGGTAATTATCTTCTTCCGTTGTTGTTAGGTATTCCTGATCTGAAACTTCCTCGTTTGAAAGCTCTTAGGGC ATTCGGTAATTATCTTCTTCCTCTTTTATTGGGTATACCTGATTTGAATCTTCCTCGTTTGAAAGCTCTTAGGGC GTTTGGTAATTACCTCCTCCCTCTTTTGCTAGGAATTCCTGATCTGAGGTTACCTCGCTTGAAAGCTCTTAGGGC GTTTGGTAATTACCTCCTCCCTCTTTTGCTAGGAATTCCTGATCTGAGGTTACCTCGCTTGAAAGCTCTTAGGGC GGGGGGTTTGGTAAATACTTGTTACCTTTATTGTTAGGATTACCTGATTTGAATTTGCCTCGTTTAAAAGCTTTA TTTGGAAAATATCTGTTGCCCCTGTTGTTGGGGCTTCCTGATTTGAATTTACCCCGTTTAAATGCCCTTAGAGCT TTTGGAAAATATCTGTTGCCCCTGTTGTTGGGGCTTCCTGATTTGAATTTACCCCGTTTAAATGCCCTTAGAGCT TTTGGAAAATATCTGTTGCCCCTGTTGTTGGGGCTTCCTGATTTGAATTTACCCCGTTTAAATGCCCTTAGAGCT TTTGGTAATTATTTGTTGCCGCTGCTGTTGGGGTTGCCGGATCTTACTTTGCCTCGGTTAAAAGCCTTTAGTGCC : : : : : : : : : : : : : : : : : : : : 300 300 300 300 300 300 300 300 300 300 300 299 296 299 299 300 300 300 300 300 FspNB-VN-c FspCB-VN-c Fhep-AU-co FspGHL-CNFgig-CN-co Fgig-ID-co Fbus(human Fbus(swine Csin-CN-co Csin-KR-co Csin-RU-co Csin-VN-co Oviv-TH-co Oviv-CN-co Ofel-RU-co Phet-VN-co Pohi-JP-co Pwes(2n)-K Pwes(3n)KR Ejap-VN-co Htai-KR-co Htai-LA-co Htai-VN-co Sfal-VN-co : : : : : : : : : : : : : : : : : : : : : : : : * 320 * 340 * 360 * AGTGCTTGGTTGTTGCTTCCTGCTTGTGTTTGTTTGTCGTTTGGTTTGATGGGGGGTATGGGTGTTGGTTGGACT AGTGCTTGGTTGTTGCTTCCTGCTTGTGTTTGTTTGTCGTTTGGTTTGATGGGGGGTATGGGTGTTGCTTGGACT AGTGCTTGGTTGTTATTTCCTGCTTGTGTTTGTTTGCCTTTTGGTTTGATAGGGGGTGTAGGTGTTGGTTGAACT AGTGCTTGGTTGTTGCTTCCTGCTTGTGTTTGTTTGTCGTTTGGTTTGATGGGGGGTATGGGTGTTGGTTGGACT AGTGCTTGGTTGTTGCTTCCTGCTTGTGTTTGTTTGTCGTTTGGTTTGATGGGGGGTATGGGTGTTGGTTGGACT AGTGCTTGGTTGTTGCTTCCTGCTTGTGTTTGTTTGTCGTTTGGTTTGATGGGGGGTATGGGTGTTGGTTGGACT AGAGTGTGGTTGTTATTGCCATCCTGCGTTTGTTTGGCTTTGAGTTTGATAGGGGGTGCTGGTGTTGGTTGAACT AGAGTGTGGTTGTTATTGCCATCCTGCGTTTGTTTGGCTTTGAGTTTGATAGGGGGTGCTGGTGTTGGTTGAACT GTGTGGTTGTTGGTTCCTTCGGCTGTTTGTTTTAGTCTTAGGATGTATGGTGGTGCTGGGGTTGGGTGGACTTTT GTGTGGTTGTTGGTTCCTTCGGCTGTTTGTTTTAGTCTTAGGATGTATGGTGGTGCTGGGGTTGGGTGGACTTTT GTGTGGTTGTTGGTTCCTTCGGCTGTTTGTTTTAGTCTTAGGATGTATGGTGGTGCTGGGGTTGGGTGGACTTTT GTGTGGTTGTTGGTTCCTTCGGCTGTTTGTTTTAGTCTTAGGATGTATGGTGGTGCTGGGGTTGGGTGGACTTTT GTTTGGTTGTTGGTTCCTTCCGCCGTCTGTTTTAGTCTGAGGATGTATGGTGGAGCTGGTGTAGGTTGAACTTTT GTTTGGTTGTTGGTTCCTTCCGCCGTCTGTTTTAGTCTGAGGATGTATGGTGGGGCTGGTGTAGGTTGAACTTTT GTGTGGTTATTGGTTCCTTCGGCTTTATGCTTTAGGTTGAGAATGTATGGTGGGGCTGGAGTTGGGTGAACTTTT TTGATTAATGCTTCCTTCGGCGGTTTGCCTTAGTGTGAGAATGTTAGGAGGTGCAGGAGTGGGGTGGACCTTTTA TTGATTAATGCTTCCTTCTGCTGTTTGTCTTAGTTTGAGCATGCTAGGAGGGGCAGGAGTAGGATGAACCTTCTA TTGGTTGATGCTTCCTTCTGCTGTCTGTCTCAGTTTGAGGATGTTAGGTGGTGCAGGTGTGGGTTGGACTTTTTA TTGGTTGATGCTTCCTTCTGCTGTCTGTCTCAGTTTGAGGATGTTAGGTGGTGCAGGTGTGGGTTGGACTTTTTA AGTGCTTGATTGTTGTTACCTTCTGCGGTTTGTTTGGGGATGAGGATGATTGGGGGTGCTGGAGTAGGTTGGACA TGGTTAATGATTCCTTCCGCTGTTTGTTTTGGGTTAAGTATGTTTGGGGGGTCTGGGGTTGGCTGGACTTTTTAT TGGTTAATGATTCCTTCCGCTGTTTGTTTTGGGTTAAGTATGTTTGGGGGGTCTGGGGTTGGCTGGACTTTTTAT TGGTTAATGATTCCTTCTGCTGTTTGTTTTGGGTTAAGTATGTTTGGGGGGTCTGGGGTTGGCTGGACTTTTTAT TGATTGATGGTTCCTGCGTCTATCAGGTTTGGTTTGAGGATGATAGGAGGATCGGGGGTTGGTTGGACTTTTTAT : : : : : : : : : : : : : : : : : : : : : : : : 375 375 375 375 375 375 375 375 375 375 375 375 375 375 375 374 371 374 374 375 375 375 375 375 FspNB-VN-c FspCB-VN-c Fhep-AU-co FspGHL-CNFgig-CN-co Fgig-ID-co Fbus(human Fbus(swine Csin-CN-co Csin-KR-co Csin-RU-co Csin-VN-co Oviv-TH-co Oviv-CN-co Ofel-RU-co Phet-VN-co Pohi-JP-co Pwes(2n)-K Pwes(3n)KR Ejap-VN-co Htai-KR-co Htai-LA-co Htai-VN-co Sfal-VN-co : : : : : : : : : : : : : : : : : : : : : : : : 380 * 400 * 420 * 440 * TTCTATCCTCCTCTTTCTAGATTGGATTATTCTGGTTGGGGGGTTGATTTTTTAATGTTTTCCCTTCATTTGGCT TTCTATCCTCCTCTTTCTAGATTGGATTTTTCTGGTTGGGGAGTTGATTTTTTAATGTTTTCTCTTCATTTGGCT TTTTATCCACCTCTTTCTAGGTTGGATTATTCTAGGTGGGGGGTTGATTTTTTGATGTTTTCTCTTCATTTGGCT TTCTATCCTCCTCTTTCTAGATTGGATTTTTCTGGTTGGGGAGTTGATTTTTTAATGTTTTCTCTTCATTTGGCT TTTTATCCCCCTCTTTCTAGTTTGGATTATTCTGGTTGGGGGGTTGATTTTTTAATGTTTTCTCTTCATTTGGCT TTCTATCCTCCTCTTTCTAGATTGGATTATTCTGGTTGGGGGGTTGATTTTTTAATGTTTTCCCTTCATTTGGCT TTTTATCCTCCTCTGTCTGCTTCAGGTTATTCTGGTTGGGGGGTGGATTTTATGATGTTTTCTTTGCATTTGGCC TTTTATCCTCCTCTGTCTGCTTCAGGTTATTCTGGTTGGGGGGTGGATTTTATGATGTTTTCTTTGCATTTGGCC TATCCTCCTCTTTCTGCTAGAGATTATAGAGGTTGAGGGGTTGATTTTTTAATGTTTTCGCTTCATTTGGCTGGG TATCCTCCTCTTTCTGCTAGAGATTATAGAGGTTGAGGGGTTGATTTTTTAATGTTTTCGCTTCATTTGGCTGGG TATCCTCCTCTTTCTGCTAGAGATTATAGAGGTTGAGGGGTTGATTTTTTAATGTTTTCGCTTCATTTGGCTGGG TATCCTCCTCTTTCTGCTAGAGATTATAGAGGTTGAGGGGTTGATTTTTTAATGTTTTCGCTTCATTTGGCTGGG TATCCCCCTCTATCTGCTGGTGATTATAGGGGCTGGGGCGTTGACTTTCTGATGTTTTCCCTTCATTTGGCTGGG TATCCCCCTCTATCTGCTGGTGATTATAGGGGCTGGGGCGTTGATTTTCTGATGTTTTCCCTTCATTTGGCTGGG TATCCTCCCCTGTCTTCTGGTGATTATAGGGGGTGGGGAGTTGATTTTTTGATGTTTTCTCTTCATTTGGCTGGA TCCCCCTCTTTCGGGTGGTGATTATTCTGGTTGGGGTACGGATTTTTTGATGTTTTCTCTTCATTTGGCTGGTCT TCCGCCTTTATCAGGTGGGGATTATTCTGGGTGAGGTACAGATTTTTTGATGTTTTCTCTTCATTTGGCGGGGTT TCCTCCTCTCTCGGGAGGTGATTACTCTGGCTGGGGTGTGGATTTTTTGATGTTCTCTCTTCATCTGGCTGGTCT TCCTCCTCTCTCGGGAGGTGATTACTCTGGCTGGGGTGTGGATTTTTTGATGTTCTCTCTTCATCTGGCTGGTCT TTTTATCCTCCTCTTTCTGGAGTAGGTTATTCAgGTTGGGGTGTGGATTTTTTAATGTTTGCTTTACATTTGGCT CCCCCCCTGTCTTCTAGGGATTATAGGGGTTGGGGTGTTGACTTTCTTATGTTTTCTTTACATTTGGCGGGGTTA CCCCCCCTGTCTTCTAGGGATTATAGGGGTTGGGGTGTTGACTTTCTTATGTTTTCTTTACATTTGGCGGGGTTA CCCCCCCTGTCTTCTAGGGATTATAGGGGTTGGGGTGTTGACTTTCTTATGTTTTCTTTACATTTGGCGGGGTTA CCTCCTCTTTCTTCGGGTGATTACAGAGGATGGGGAGTGGATTTTCTTATGTTTTCTCTGCATCTTGCTGGGCTG : : : : : : : : : : : : : : : : : : : : : : : : 450 450 450 450 450 450 450 450 450 450 450 450 450 450 450 449 446 449 449 450 450 450 450 450 FspNB-VN-c FspCB-VN-c Fhep-AU-co FspGHL-CNFgig-CN-co Fgig-ID-co Fbus(human Fbus(swine Csin-CN-co Csin-KR-co Csin-RU-co Csin-VN-co Oviv-TH-co Oviv-CN-co : : : : : : : : : : : : : : 460 * 480 * 500 * 520 GGTGTTTCTAGTCTTTTGGGTTCTATTAAATTTATTTGTACTATTTTGGAGGTTATGTTGGGCGAGGGTATTGGT GGTGTTTCTAGTCTTTTGCGTTCTATTAAATTTATTTGTACTATTTTGGAGGTTATGTTGGGTGAGGGTATTGGT GGGGTTTCTAGTCTTTTAGGTTCTATTAAATTTATTTGTACTATTTTAGAGGTTATGTTGGATGAGGGTACGGGT GGTGTTTCTAGTCTTTTGGGTTCTATTAAATTTATTTGTACTATTTTGGAGGTTATGTTGGGTGAGGGTATTGGT GGTGTTTCTAGTCTTTTGGGTTCTATTAAATTTATTTGTACTATTTTGGAGGTTATGTTGGGCGAGGGTACTGGT GGTGTTTCTAGTCTTTTGGGTTCTATTAAATTTATTTGTACTATTTTGGAGGTTATGTTGGGTGAGGGTATTGGT GGGGTATCTAGTATTTTTAGTTCTATTAAATTTATTTGTACTATAGTTGAGGTTATGTTTGAGGAAGGTACAGGT GGGGTATCTAGTATTTTTAGTTCTATTAAATTTATTTGTACTATAGTTGAGGTTATGTTTGAGGAAGGTACAGGT TTATCGAGTCTTTTTGGATCTTTGAAATTTATTTGTACAATATGGTCTGCCGCGGATAGGCGGGTGAGGGAACGA TTATCGAGTCTTTTTGGATCTTTGAAATTTATTTGTACAATATGGTCTGCCGCGGATAGGCGGGTGAGGGAACGA TTATCGAGTCTTTTCGGATCTTTGAAATTTATTTGTACAATATGGTCTGCCGCGGATAGGCGGGTGAGGGAACGA TTATCGAGTCTTTTTGGATCTTTGAAATTTATTTGTACAATATGGTCTGCCGCGGATAGGCGGGTGAGGGAACGA TTATCTAGTCTTTTTGGGTCCTTGAAATTTATTTGTACTATATGGTCAGCAATGGATAGTCGGATTTTGGAGCGT TTATCTAGTCTTTTTGGGTCCTTGAAATTTATTTGTACTATATGGTCAGCAATGGATAGTCGGATTTTGGAGCGT : : : : : : : : : : : : : : 525 525 525 525 525 525 525 525 525 525 525 525 525 525 67 Ofel-RU-co Phet-VN-co Pohi-JP-co Pwes(2n)-K Pwes(3n)KR Ejap-VN-co Htai-KR-co Htai-LA-co Htai-VN-co Sfal-VN-co : : : : : : : : : : TTGTCTAGTCTTTTTGGGTCTTTGAATTTTATCTGTACTATTTGGTCTGCCATGGATAAGCGGGTTTTGGAGCGT TTCTAGTCTTTTTGGTTCGTTGAAATTTATTTGTACTATCGTCAGGGCGTTGTGTGAGCATACTGTGGGTCGGAG ATCTAGTCTTTTGGGTTCCTTAAATTTTATTTGTACTATTGTTAGGGCGTTATGCGAGCATACGGTGGGTCGTAG CTCGAGCCTTCTTGGTTCCTTGAAATTCATTTGCACCATTGTTAGGGCGTTGTGTGAGCACACTGTGGGTCGCAG CTCGAGCCTTCTTGGTTCCTTGAAATTCATTTGCACCATTGTTAGGGCGTTGTGTGAGCACACTGTGGGTCGCAG GGTGTCTCTAGTTTACTTGGTTCAATAaATTTTATTTGTACAATTGTGGAGGTGATGTTGGAGGAGAGTACTGCT TCTAGTCTGTTTGGTTCTTTAAACTTTATTTGTACAATAATGAGGGCTATGAGTAATGATACCAGGGAGCGGTAC TCTAGTCTGTTTGGTTCTTTAAACTTTATTTGTACAATAATGAGGGCTATGAGTAATGATACCAGGGAGCGGTAC TCTAGTCTGTTTGGTTCTTTAAACTTTATTTGTACAATAATGAGGGCTATGAGTAATGATACCAGGGAGCGGTAC TCTAGGGTGTTTGGGTCACTGAATTTTGTTTGTACTATCCTGGAGGTGATGCTGGGAAGGATGAGCGAGCGGTAT : : : : : : : : : : 525 524 521 524 524 525 525 525 525 525 FspNB-VN-c FspCB-VN-c Fhep-AU-co FspGHL-CNFgig-CN-co Fgig-ID-co Fbus(human Fbus(swine Csin-CN-co Csin-KR-co Csin-RU-co Csin-VN-co Oviv-TH-co Oviv-CN-co Ofel-RU-co Phet-VN-co Pohi-JP-co Pwes(2n)-K Pwes(3n)KR Ejap-VN-co Htai-KR-co Htai-LA-co Htai-VN-co Sfal-VN-co : : : : : : : : : : : : : : : : : : : : : : : : * 540 * 560 * 580 * 600 CGTCATAGTATTTTAGTTTGGGCTTATTTGTTTACTTCTATTTTGTTGTTATTGTCTTTGCCGGTTTTAGCTGCT CGTCATAGTATTTTAGTTCGGGCTTATTTGTTTACTTCTATTTTGTTGTTATTGTCTTTGCCGGTTTTAGCTGCT CGGCATAGTATTTTGGTTTGGGCTTATCTATTTACTTCTGTTTTATTGTTGTTGTCATTGCCTGTTTTGGCTGCT CGTCATAGTATTTTAGTTCGGGCTTATTTGTTTACTTCTATTTTGTTGTTATTGTCTTTGCCGGTTTTAGCTGCT CGTCATAGTATTTTAGTTTGGGCTTATTTGTTTACTTCTATTTTGTTGTTGTTGTCTTTGCCGGTTTTAGCTGCT CGTCATAGTATTTTAGTTTGGGCTTATTCGTTTACTTCTATTTTGTTGTTATTGTCTTTGCCGGTTTTAGCTGCT CGTTTGAGTATTTTGGTTTGGGCTTATTTGTTTACTTCTATTTTATTGCTTTTATCTTTGCCTGTATTAGCTGCT CGTTTGAGTATTTTGGTTTGGGCTTATTTGTTTACTTCTATTTTATTGCTTTTATCTTTGCCTGTATTAGCTGCT CACTCTATAATAGTTTGGGCTTATTTGTTTACGTCTATCTTGTTGTTGGGTACGTTGCCTGTTTTAGCTGCTGGT CACTCTATAATAGTTTGGGCTTATTTGTTTACGTCTATCTTGTTGTTGGGTACGTTGCCTGTTTTAGCTGCTGGT CACTCTATAATAGTTTGGGCTTATTTGTTTACGTCTATCTTGTTGTTGGGTACGTTGCCTGTTTTAGCTGCTGGT CACTCTATAATAGTTTGGGCTTATTTGTTTACGTCTATCTTGTTGTTGGGTACGTTGCCTGTTTTAGCTGCTGGT CATTCAATTATAGTGTGGGCTTATATGTTTACTTCAATTTTGTTGCTGGGTACGTTGCCCGTTTTGGCGGCTGGT CATTCAATTATAGTGTGGGCTTATATGTTTACTTCAATTTTGTTGCTGGGTACGTTGCCCGTTTTGGCGGCTGGT CATTCTATCATAATTTGGGCTTATCTATTTACTTCTTTTTTGTTGTTGGGTACATTGCCTGTTTTGGCGGCTGCT GTCTATAATTGTTTGGGCCTATCTTTTTACGTCTATCTTGTTGCTTCTTTCTTTGCCGGTGCTTGCTGCTGCGAT GTCTATAATTGTTTGGGCGTATCTTTTTACGTCTGTTTTGTTACTACTTTCTTTACCAGTATTAGCGGCTGCAAT GTCAATAATTGTCTGGGCTTATCTCTTCACCTCTGTTCTGCTGCTCCTCTCTTTACCTGTGCTTGCTGCTGCGAT GTCAATAATTGTCTGGGCTTATCTCTTCACCTCTGTTCTGCTGCTCCTCTCTTTACCTGTGCTTGCTGCTGCGAT CGTCATAGGGTGTTGGTTTGAGGGTATTTGTTCACGTCGATCTTGTTGTTGCTtTCTTTGCCTGtATTAGCGGCT TCTATTGTGGTTTGGGCTTATTTGTTTACTTCTATTCTTTTGTTGCTTTCGTTACCAGTGCTAGCTGCGGGTATA TCTATTGTGGTTTGGGCTTATTTGTTTACTTCTATTCTTTTGTTGCTTTCGTTACCAGTGCTAGCTGCGGGTATA TCTATTGTGGTTTGGGCTTATTTGTTTACTTCTATTCTTTTGTTGCTTTCGTTACCAGTGCTAGCTGCGGGTATA TCAATCATTGTTTGGGCGTATCTTTTTACTTCAATTTTGCTTTTGTTGTCGTTGCCTGTGTTGGCTGCGGGTATT : : : : : : : : : : : : : : : : : : : : : : : : 600 600 600 600 600 600 600 600 600 600 600 600 600 600 600 599 596 599 599 600 600 600 600 600 FspNB-VN-c FspCB-VN-c Fhep-AU-co FspGHL-CNFgig-CN-co Fgig-ID-co Fbus(human Fbus(swine Csin-CN-co Csin-KR-co Csin-RU-co Csin-VN-co Oviv-TH-co Oviv-CN-co Ofel-RU-co Phet-VN-co Pohi-JP-co Pwes(2n)-K Pwes(3n)KR Ejap-VN-co Htai-KR-co Htai-LA-co Htai-VN-co Sfal-VN-co : : : : : : : : : : : : : : : : : : : : : : : : * 620 * 640 * 660 * GCTATTACTATGTTGTTGTTTGATCGTAAATTTAGTTCTGCTTTTTTTGATCCTATGGGGGGTGGCGATCCTGTC GCTATTACTATGTTGTTGTTTGATCGTAAATTTAGTTCTGCTTTTTTTGATCGTATGGGGGGTGGTGATCCTGTC GCTATTACTATGTTGTTATTTGATCGTAAATTTGGTTCTGCTTTTTTTGATCCTATGGGAGGTGGGGATCCTGTT GCTATTACTATGTTGTTGTTTGATCGTAAATTTAGTTCTGCTTTTTTTGATCCTATGGGGGGTGGTGATCCTGTC GCTATTACTATGTTGTTGTTTGATCGTAAATTTAGTTCTGCTTTTTTTGATCCTATGGGGGGTGGCGATCCTGTC GCTATTACTATGTTGTTGTTTGATCGTAAATTTAGTTCTGCTTTTTTTGATCCTATGGGGGGTGGCGATCCTGTC GCTATAACTATGTTATTGTTTGATCGTAGTTTTGGGTCTGCATTTTTTGATCCTATGGGGGGGGGAGATCCGGTG GCTATAACTATGTTATTGTTTGATCGTAGTTTTGGGTCTGCATTTTTTGATCCTATGGGGGGGGGAGATCCGGTG ATAACTATGCTTCTTTTTGATCGTAAATTTGGTACAGTGTTTTTTGATCCGTCGGGTGGGGGTGATCCTTTGTTG ATAACTATGCTTCTTTTTGATCGTAAATTTGGTACAGTGTTTTTTGATCCGTCGGGTGGGGGTGATCCTTTGTTG ATAACTATGCTTCTTTTTGATCGTAAATTTGGTACAGTGTTTTTTGATCCGTCGGGTGGGGGTGATCCTTTGTTG ATAACTATGCTTCTTTTTGATCGTAAATTTGGTACAGTGTTTTTTGATCCGTCGGGTGGGGGTGATCCTTTGTTG ATAACTATGCTTCTTTTTGATCGTAAATTTGGTACTGTGTTTTTTGATCCGTCTGGGGGTGGTGATCCGTTGTTG ATAACTATGCTTCTTTTTGATCGTAAATTTGGTACTGTGTTTTTTGATCCGTCTGGGGGTGGTGATCCGTTGTTG ATAACCATGCTTCTTTTTGATCGTAATTTTGGTACTGTGTTTTTTGATCCATCCGGTGGGGGTGATCCTGTGTTA TACCATGCTTTTGTTTGATCGAAAATTTAGTTCTGCTTTTTTTGACCCCCTTGGTGGTGGTGATCCTGTTTTATT TACCATGCTTTTGTTTGATCGTAAATTTAGTTCTGCTTTTTTTGATCCCTTGGGTGGAGGTGATCCTGTTTTGTT CACCATGCTTTTGTTCGATCGGAAATTTAGTTCTGCCTTTTTTGATCCGTTGGGGGGTGGAGATCCCGTTTTGTT CACCATGCTTTTGTTCGATCGGAAATTTAGTTCTGCCTTTTTTGATCCGTTGGGGGGTGGAGATCCCGTTTTGTT GCTATTACGATGTTGTTGTTTGATCGTAAATTTGGGGCTTCATTTTTTGATCCGATGGGAGGTGGGGATCCTGTG ACGATGCTTTTGTTTGATCGTAAATTTAGCTCTTCTTTTTTTGATCCATTGGGAGGGGGGGACCCTGTCTTGTTC ACGATGCTTTTGTTTGATCGTAAATTTAGCTCTTCTTTTTTTGATCCATTGGGAGGGGGGGACCCTGTCTTGTTC ACGATGCTTTTGTTTGATCGTAGATTTAGCTCTTCTTTTTTTGATCCATTGGGAGGGGGGGACCCTGTCTTGTTC ACCATGCTTCTTTTTGATCGTAACTTTGGTTCTGCCTTTTTTGATCCTTTGGGTGGGGGAGACCCCGTTTTGTTC : : : : : : : : : : : : : : : : : : : : : : : : 675 675 675 675 675 675 675 675 675 675 675 675 675 675 675 674 671 674 674 675 675 675 675 675 FspNB-VN-c FspCB-VN-c Fhep-AU-co FspGHL-CNFgig-CN-co Fgig-ID-co Fbus(human Fbus(swine Csin-CN-co Csin-KR-co Csin-RU-co Csin-VN-co Oviv-TH-co Oviv-CN-co Ofel-RU-co Phet-VN-co Pohi-JP-co Pwes(2n)-K Pwes(3n)KR Ejap-VN-co Htai-KR-co Htai-LA-co Htai-VN-co : : : : : : : : : : : : : : : : : : : : : : : 680 * 700 * 720 * 740 * TTATTTCAGCATTTGTTTTGGTTTTTTGGTCATCCGGAGGTTTATGTTTTAATTTTGCCTGGGTTTGGGGTTATT TTATTTCAGCATTTGTTTTGGTTTATTGGTCATCCGGAAGTTTATGCTTTGATTTTGCCTGGGTTTGGAGTTATT TTATTTCAGCATTTGTTTTGGTTTTTTGGGCATCCTGAGGTTTATGTTTTGATTTTACCCGGGTTTGGGGTTATT TTATTTCAGCATTTGTTTTGGTTTATTGGTCATCCGGAAGTTTATGCTTTGATTTTGCCTGGGTTTGGAGTTATT TTATTTCAGCATTTGTTTTGGTTTTTTGGTCATCCGGAGGTTTATGTTTTAATTTTGCCTGGGTTTGGGGTTATT TTATTTCAGCATTTGTTTTGGTTTTTTGGTCATCCGGAGGTTTATGTTTTAATTTTGCCTGGGTTTGGGGTTATT TTGTTTCAGCATTTGTTTTGGTTTTTTGGGCATCCCGAGGTGTATGTTTTGATTTTGCCAGGGTTTGGTGTGATT TTGTTTCAGCATTTGTTTTGGTTTTTTGGGCATCCCGAGGTGTATGTTTTGATTTTGCCAGGGTTTGGTGTGATT TTTCAGCATATGTTTTGGTTTTTTGGTCATCCTGAGGTGTATGTGTTAATATTGCCGGGGTTTGGTATGATTAGT TTTCAGCATATGTTTTGGTTTTTTGGTCATCCTGAGGTGTATGTGTTAATATTGCCGGGGTTTGGTATGATTAGT TTTCAGCATATGTTTTGGTTTTTTGGTCATCCTGAGGTGTATGTGTTAATATTGCCGGGGTTTGGTATGATTAGT TTTCAGCATATGTTTTGGTTTTTTGGTCATCCTGAGGTGTATGTGTTAATATTGCCGGGGTTTGGTATGATTAGT TTTCAACATATGTTTTGGTTTTTCGGTCATCCGGAAGTATACGTGTTGATTTTGCCAGGATTCGGTATGATTAGT TTTCAACATATGTTTTGGTTTTTCGGTCATCCGGAAGTATACGTGTTGATTTTGCCAGGATTCGGTATGATTAGT TTTCAGCATATGTTTTGGTTTTTTGGTCATCCGGAGGTGTATGTGTTAATATTGCCGGGGTTTGGAATGATTAGT TCAACACCTTTTTTGGTTTTTTGGCCATCCGGAGGTGTATGTTTTAATTTTGCCTGGATTTGGTGTTGTGAGACA CCAGCATTTGTTTTGGTTTTTTGGGCATCCTGAGGTTTATGTTTTAATTTTACCAGGATTTGGGATTGTGAGACA TCAGCATTTGTTTTGGTTCTTTGGGCATCCGGAGGTGTATGTCCTGATTTTGCCTGGGTTTGGTATCGTGAGCCA TCAGCATTTGTTTTGGTTCTTTGGGCATCCGGAGGTGTATGTCCTGATTTTGCCTGGGTTTGGTATCGTGAGCCA TTGTTTCAGCATTTATTTTGGTTTTTTGGGCATCCTGAGGTTTATGTTTTAATTTTGCCTGCTTTTGGAGTAATT CAGCATATGTTTTGGTTTTTTGGGCACCCCGAAGTTTACGTTCTTATCCTCCCAGGGTTTGGTATAGTGAGACAC CAGCATATGTTTTGGTTTTTTGGGCACCCCGAAGTTTACGTTCTTATCCTCCCAGGGTTTGGTATAGTGAGACAC CAGCATATGTTTTGGTTTTTTGGGCACCCCGAAGTTTACGTTCTTATTCTCCCAGGGTTTGGTATAGTGAGACAC : : : : : : : : : : : : : : : : : : : : : : : 750 750 750 750 750 750 750 750 750 750 750 750 750 750 750 749 746 749 749 750 750 750 750 68 Sfal-VN-co : CAGCATATGTTTTGATTCTTTGGTCATCCCGAGGTTTACGTTTTGATTCTCCCTGGGTTTGGTATTGTTAGTCAT : 750 FspNB-VN-c FspCB-VN-c Fhep-AU-co FspGHL-CNFgig-CN-co Fgig-ID-co Fbus(human Fbus(swine Csin-CN-co Csin-KR-co Csin-RU-co Csin-VN-co Oviv-TH-co Oviv-CN-co Ofel-RU-co Phet-VN-co Pohi-JP-co Pwes(2n)-K Pwes(3n)KR Ejap-VN-co Htai-KR-co Htai-LA-co Htai-VN-co Sfal-VN-co : : : : : : : : : : : : : : : : : : : : : : : : 760 * 780 * 800 * 820 AGTCATATTTGTATGACTTTGACTAATAATGATTCTTTATTTGGTTATTATGGTCTTATTTTGGCCATGGCTGCT AGTCATATTTGTATGACTTTGACTAATAATGATTCTTTATTTGGTTATTATGGTCTTATTTTGGCTATGGCTGCT AGTCATATTTGTGTGACTTTAACTAATAATGATTCTTTGTTTGGTTATTATGGTCTTATTTTAGCTATGGCTGCT AGTCATATTTGTATGACTTTGACTAATAATGATTCTTTATTTGGTTATTATGGTCTTATTTTGGCTATGGCTGCT AGCCATATTTGTATGACTTTGACTAATAATGATTCTTTATTTGGTTATTATGGTCTTATTTTGGCTATGGCTGCT AGTCATATTTGTATGACTTTGACTAATAATGATTCTTTATTTGGTTATTATGGTCTTATTTTGGCTATGGCTGCT AGACATATTTGTGTTACTTTGACTAATAAAGATTCTTTGTTTGGTTATTATGGGCTTGTTTTGGCTATGGCTGCT AGACATATTTGTGTTACTTTGACTAATAAAGATTCTTTGTTTGGTTATTATGGGCTTGTTTTGGCTATGGCTGCT CACATTTGTACTACTTTAACAGGTAAAGATTCGTTGTTTGGTTATGGGGGCTTGGTGTTGGCTATGTTTGCTGTA CACATTTGTACTACTTTAACAGGTAAAGATTCGTTGTTTGGTTATGGGGGCTTGGTGTTGGCTATGTTTGCTGTA CACATTTGTACTACTTTAACAGGTAAAGATTCGTTGTTTGGTTATGGGGGCTTGGTGTTGGCTATGTTTGCTATA CACATTTGTACTACTTTAACAGGTAAAGATTCGTTGTTTGGTTATGGGGGCTTGGTGTTGGCTATGTTTGCTATA CATATCTGTACTACGTTAACTGGTAATGATTCTTTGTTCGGTTATGGGGGTTTGGTGTTGGCCATGTTTGCTATA CATATCTGTACTACGTTAACTGGTAATGATTCTTTGTTCGGTTATGGTGGTTTGGTGTTGGCCATGTTTGCTATA CATGTTTGTACGACTCTAACAGGGAAAGATTCCCTATTTGGTTATGGTGGTTTGGTGTTAGCCATGTTTGCTATA TATCTGCATGACTTTGACTAATAAAGATTCTTTGTTTGGTTATTATGGCTTGGTTTTTGCTATGGGGGCTATTGT TATTTGTATGACTCTAACTAATAAAGATTCGTTGTTTGGTTATTATGGATTGGTGTTTGCTATGGGGGCGATTGT CATTTGCATGACCCTGACCAACAACGATTCCTTGTTCGGGTACTACGGGCTGGTGTTTGCGATGGGGGCCATTGT CATTTGCATGACCCTGACCAACAACGATTCCTTGTTCGGGTACTACGGGCTGGTGTTTGCGATGGGGGCCATTGT AGGCATATTTGTGTAACGCTGACTAATAAAGATTCTTTATTTGGTTATTATGGTTTGGTGTTAGCTATGGCGGCT ATTTGTAGGACGTTAACAAATAAAGATTCCTTGTTTGGTTATGGGGGTTTAGTTCTTGCTATGTTTTCTATAGTC ATTTGTAGGACGTTAACAAATAAAGATTCCTTGTTTGGTTATGGGGGTTTAGTTCTTGCTATGTTTTCTATAGTC ATTTGTAGGACGTTAACAAATAAAGATTCCTTGTTTGGTTATGGGGGTTTAGTTCTTGCTATGTTTTCTATAGTC GTTTGCATGACTCTAACGAACAATGATTCCTTGTTTGGTTACGGGGGTCTGGTCCTTGCTATGTTTTCTATTGTC : : : : : : : : : : : : : : : : : : : : : : : : 825 825 825 825 825 825 825 825 825 825 825 825 825 825 825 824 821 824 824 825 825 825 825 825 FspNB-VN-c FspCB-VN-c Fhep-AU-co FspGHL-CNFgig-CN-co Fgig-ID-co Fbus(human Fbus(swine Csin-CN-co Csin-KR-co Csin-RU-co Csin-VN-co Oviv-TH-co Oviv-CN-co Ofel-RU-co Phet-VN-co Pohi-JP-co Pwes(2n)-K Pwes(3n)KR Ejap-VN-co Htai-KR-co Htai-LA-co Htai-VN-co Sfal-VN-co : : : : : : : : : : : : : : : : : : : : : : : : * 840 * 860 * 880 * 900 ATAGTATGTTTGGGTAGTGTTGTTTGGGCTCATCATATGTTTATGGTGGGTTTGGATGTACATACTGCTGTTTTT ATAGTATGTTTGGGTAGTGTTGTTTGGGCTCATCATATGTTTATGGTGGGTTTGGATGTACATACTGCTGTTTTT ATAGTATGTTTAGGTAGTGTTGTTTGGGCTCATCATATGTTTATGGTGGGTTTGGATGTGCATACTGCTGTTTTT ATAGTATGTTTGGGTAGTGTTGTTTGGGCTCATCATATGTTTATGGTGGGTTTGGATGTACATACTGCTGTTTTT ATAGTATGTTTGGGTAGTGTTGTTTGGGCTCATCATATGTTTATGGTGGGTTTGGATGTACATACTGCTGTTTTT ATAGTATGTTTGGGTAGTGTTGTTTGGGCTCATCATATGTTTATGGTGGGTTTGGATGTACATACTGCTGTTTTT ATAGTTTGTTTGGGTAGTATAGTTTGGGCACATCATATGTTTATGGTTGGTTTGGATGTGCATACTGCTGTTTTT ATAGTTTGTTTGGGTAGTATAGTTTGGGCACATCATATGTTTATGGTTGGTTTGGATGTGCATACTGCTGTTTTT GTTTGTCTGGGTAGGGTGGTTTGAGCTCATCATATGTTTACTGTTGGGCTGGATTTGGGGACTGCTGTTTTTTTT GTTTGTCTGGGTAGGGTGGTTTGAGCTCATCATATGTTTACTGTTGGGCTGGATTTGGGGACTGCTGTTTTTTTT GTTTGTCTGGGTAGGGTGGTTTGAGCTCATCATATGTTTACTGTTGGGCTGGATTTGGGGACTGCTGTTTTTTTT GTTTGTCTGGGTAGGGTGGTTTGAGCTCATCATATGTTTACTGTTGGGCTGGATTTGGGGACTGCTGTTTTTTTT GTCTGTTTAGGAAGGGTGGTTTGGGCTCATCATATGTTTACGGTTGGGCTGGATTTGGGCACCGCTGTGTTTTTT GTCTGTTTAGGAAGGGTGGTTTGGGCTCATCATATGTTTACGGTTGGGCTGGATTTGGGCACCGCTGTGTTTTTT GTTTGTTTGGGTAGTGTGGTTTGAGCTCATCATATGTTTACTGTAGGATTAGATTTAGGGACTGCTATTTTTTTT GTGTTTGGGGAGGGTTGTTTGAGCGCACCACATGTTTATGGTTGGTTTAGATGTTAAGACTGCTGTTTTTTTTAG GTGTTTGGGAAGTGTTGTCTGGGCCCACCATATGTTTATGGTTGGTTTGGATGTTAAGACTGCAGTATTTTTCAG GTGTCTGGGTAGTGTTGTGTGGGCGCATCACATGTTCATGGTTGGTTTGGATGTCAAGACTGCTGTTTTCTTTAG GTGTCTGGGTAGTGTTGTGTGGGCGCATCACATGTTCATGGTTGGTTTGGATGTCAAGACTGCTGTTTTCTTTAG ATTGTGTGTCTGGGTAGTATAGTTTGGGCACATCATATGTTTATGGTTGGGTTGGATATAGATACCGCGGTGTTT TGTTTGGGGAGTGTTGTTTGAGCTCATCATATGTTTATGGTTGGGTTGGATGTTAAGACGGCTGTTTTTTTTAGT TGTTTGGGGAGTGTTGTTTGAGCTCATCATATGTTTATGGTTGGGTTGGATGTTAAGACGGCTGTTTTTTTTAGT TGTTTGGGGAGTGTTGTTTGAGCTCATCATATGTTTATGGTTGGGTTGGATGTTAAGACGGCTGTTTTTTTTAGT TGTCTGGGGAGTATTGTTTGGGCACATCATATGTTTATGGTAGGTCTGGATTTGAAGACTGCGGTATTTTTTAGG : : : : : : : : : : : : : : : : : : : : : : : : 900 900 900 900 900 900 900 900 900 900 900 900 900 900 900 899 896 899 899 900 900 900 900 900 FspNB-VN-c FspCB-VN-c Fhep-AU-co FspGHL-CNFgig-CN-co Fgig-ID-co Fbus(human Fbus(swine Csin-CN-co Csin-KR-co Csin-RU-co Csin-VN-co Oviv-TH-co Oviv-CN-co Ofel-RU-co Phet-VN-co Pohi-JP-co Pwes(2n)-K Pwes(3n)KR Ejap-VN-co Htai-KR-co Htai-LA-co Htai-VN-co Sfal-VN-co : : : : : : : : : : : : : : : : : : : : : : : : * 920 * 940 * 960 * TTTAGTTCTGTTACTATGGTTATTGGTATTCCTACAGGGATTAAGGTTTTTTCTTGGTTAATAATGTTGGGGGGT TTTAGTTCTGTTACTATGGTTATTGGTATCCCTACCGGGATTAAGGTTTTTTCTTGGTTAATAATGTTGGGGGGT TTTAGTTCTGTTACTATGGTTATTGGTATTCCTACAGGTATTAAGGTCTTTTCCTGGTTGATAATGTTGGGGGGG TTTAGTTCTGTTACTATGGTTATTGGTATCCCTACCGGGATTAAGGTTTTTTCTTGGTTAATAATGTTGGGGGGT TTTAGTTCTGTTACTATGGTTATTGGTATTCCTACTGGGATTAAGGTTTTCTCTTGATTAATAATGTTGGGGGGT TTTAGTTCTGTTACTATGGTTATTGGTATTCCTACAGGGATTAAGGTTTTTTCTTGGTTAATAATGTTGGGGGGT TTTAGTTCTGTTACTATGGTGATTAGTATTCCCACAGGTATTAAGGTTTTTTCTTGGTTGATAATGTTAGGGGGG TTTAGTTCTGTTACTATGGTGATTAGTATTCCCACAGGTATTAAGGTTTTTTCTTGGTTGATAATGTTAGGGGGG AGCTCGGTTACTATGATTATAGGTGTGCCTACGGGGATCAAGGTTTTTTCATGATTATATATGCTTGCTGGAACT AGCTCGGTTACTATGATTATAGGTGTGCCTACGGGGATCAAGGTTTTTTCATGATTATATATGCTTGCTGGAACT AGCTCGGTTACTATGATTATAGGTGTGCCTACGGGGATCAAGGTTTTTTCATGATTATATATGCTTGCTGGAACT AGCTCGGTTACTATGATTATAGGTGTGCCTACGGGGATCAAGGTTTTTTCATGATTATATATGCTTGCTGGAACT AGCTCGGTTACTATGATTATAGGGGTTCCTACTGGAATAAAGGTTTTTTCTTGGTTGTATATGCTTGCGGGTACT AGCTCGGTTACTATGATTATAGGGGTTCCTACTGGAATAAAGGTTTTTTCTTGGTTGTATATGCTTGCGGGTACT AGTTCAGTTACTATGATCATTGGTGTACCTACAGGGATAAAGGTTTTTTCTTGATTATACATGCTTGCCGGTACT TTCTGTTACTGGGGTGATTGGGATTCCCACAGGGATTAAGGTTTTTTCTTGGTTGTTTATGTTGGGGGGGACTCG ATCTGTTACGGGGGTGATAGGTATTCCTACAGGGATTAAGGTTTTTTCTTGGTTATTTATGTTAGGGGGTACTCG TTCTGTCACGGGAGTGATTGGGATACCCACGGGGATTAAGGTTTTCTCTTGGTTGTTCATGCTGGGTGGAACTCG TTCTGTCACGGGAGTGATTGGGATACCCACGGGGATTAAGGTTTTCTCTTGGTTGTTCATGCTGGGTGGAACTCG TTTAGGTCTGTGACGATGGTTATTGGTATCCCTACAGGCATTAAGGTTTTTTCTTGGTTGGTTATGTTAAGTGGT TCTGTGACCATGGTTATAGGAGTCCCCACAGGTATAAAGGTTTTTTCTTGGCTGTATATGTTGGCGGGAAGTCGG TCTGTGACCATGGTTATAGGAGTCCCCACAGGTATAAAGGTTTTTTCTTGGCTGTATATGTTGGCGGGAAGTCGG TCTGTGACCATGGTTATAGGAGTCCCCACAGGTATAAAGGTTTTTTCTTGGCTGTATATGTTGGCGGGAAGTCGG TCTGTAACTATGATTATCGGTGTTCCTACAGGTATTAAGGTGTTTTCGTGGTTGTATATGTTGGCTGGAAGCCGT : : : : : : : : : : : : : : : : : : : : : : : : 975 975 975 975 975 975 975 975 975 975 975 975 975 975 975 974 971 974 974 975 975 975 975 975 FspNB-VN-c FspCB-VN-c Fhep-AU-co FspGHL-CNFgig-CN-co Fgig-ID-co Fbus(human : : : : : : : 980 * 1000 * 1020 * 1040 * GGTGGTTCTGTTCGTATTTGGGATCCTGTTATATGATGGATTGTTGGTTTTGTTGTTTTGTTTACTATTGGTGGG GGTAGTTCTGTTCGTATTTGGGATCCTGTTGTATGATGGATTGTTGGTTTTGTTGTTTTGTTTACTATTGGTGGG GGTAGTTCTGTTCGTATATGGGATCCTGTTGTGTGGTGAATTATAGGGTTTATTGTTTTATTTACTATTGGTGGG GGTAGTTCTGTTCGTATTTGGGATCCTGTTGTATGATGGATTGTTGGTTTTGTTGTTTTGTTTACTATTGGTGGG GGTAGTTCTGTTCGTATTTGGGATCCTGTTGTATGATGGATTGTTGGTTTTGTTGTTTTGTTTACTATTGGTGGG GGTGGTTCTGTTCGTATTTGGGATCCTGTTATATGATGGATTGTTGGTTTTGTTGTTTTGTTTACTATTGGTGGG GGTAGATTTGTTCGTATTTGGGATCCCATAGTGTGATGAATTGTGGGGTTTGTTGTGTTGTTTACTATAGGGGGG : : : : : : : 1050 1050 1050 1050 1050 1050 1050 69 Fbus(swine Csin-CN-co Csin-KR-co Csin-RU-co Csin-VN-co Oviv-TH-co Oviv-CN-co Ofel-RU-co Phet-VN-co Pohi-JP-co Pwes(2n)-K Pwes(3n)KR Ejap-VN-co Htai-KR-co Htai-LA-co Htai-VN-co Sfal-VN-co : : : : : : : : : : : : : : : : : GGTAGATTTGTTCGTATTTGGGATCCCATAGTGTGATGAATTGTGGGGTTTGTTGTGTTGTTTACTATAGGGGGG CGGGAGCGTCTATGAGACCCAATCATGTGGTGGATAATCGGGTTTGTGGTGCTTTTCACTATAGGCGGGGTTACT CGGGAGCGTCTATGAGACCCAATCATGTGGTGGATAATCGGGTTTGTGGTGCTTTTCACTATAGGCGGGGTTACT CGGGAGCGTCTATGAGATCCAATCATGTGGTGGATAATCGGGTTTGTGGTGCTTTTCACTATAGGCGGGGTTACT CGGGAGCGTCTATGAGATCCAATCATGTGGTGGATAATCGGGTTTGTGGTGCTTTTCACTATAGGCGGGGTTACT CGTGAGCGTTTTTGGGACCCGATTATGTGGTGGATAGTTGGGTTCGTAGTACTTTTTACTATAGGGGGGGTTACC CGTGAGCGTTTTTGGGACCCGATTATGTGGTGGATAGTTGGGTTCGTAGTACTTTTTACTATAGGGGGGGTTACC CGAGATCGTCTTTGGGATCCGATTATGTGGTGGATAATCGGATTTATAGTGCTTTTTACTATAGGTGGGGTTACT TTTACGGTTTTGAGATCCGGTGGTTTGGTGAATTTTAGGCTTTATTTTTCTTTTTACTATTGGTGGTGTAACTGG TCTACGGTTTTGGGATCCTGTAATATGATGAATTCTTGGGTTTATCTTTTTGTTTACAATAGGGGGTGTAACTGG TTTGCGGTTTTGGGATCCTGTTTTGTGGTGAATTCTTGGGTTTATCTTCCTGTTCACCATAGGTGGTGTGACCGG TTTGCGGTTTTGGGATCCTGTTTTGTGGTGAATTCTTGGGTTTATCTTCCTGTTCACCATAGGTGGTGTGACCGG AGGATGTCTCGTTTAGGGGACCCTATAATTTGGTGGATTGTGGGGTTTATAGGATTGTTCACTATAGGGGGAGTG GGCCGGTTTTGGGATCCGATAATGTGGTGGATATTGGGTTTTATTGTCTTATTTACTATCGGGGGGGTGACCGGG GGCCGGTTTTGGGATCCGATAATGTGGTGGATATTGGGTTTTATTGTCTTATTTACTATCGGGGGGGTGACCGGG GGCCGGTTTTGGGATCCGATAATGTGGTGGATATTGGGTTTTATTGTCTTATTTACTATCGGGGGGGTGACCGGG GGCCGTTTTTGAGATCCGGTGATGTGGTGGATACTGGGATTCATCGTCTTGTTTACCATCGGGGGTATTACGGGT : : : : : : : : : : : : : : : : : 1050 1050 1050 1050 1050 1050 1050 1050 1049 1046 1049 1049 1050 1050 1050 1050 1050 FspNB-VN-c FspCB-VN-c Fhep-AU-co FspGHL-CNFgig-CN-co Fgig-ID-co Fbus(human Fbus(swine Csin-CN-co Csin-KR-co Csin-RU-co Csin-VN-co Oviv-TH-co Oviv-CN-co Ofel-RU-co Phet-VN-co Pohi-JP-co Pwes(2n)-K Pwes(3n)KR Ejap-VN-co Htai-KR-co Htai-LA-co Htai-VN-co Sfal-VN-co : : : : : : : : : : : : : : : : : : : : : : : : 1060 * 1080 * 1100 * 1120 GTTACTGGTATTATGCTTTCTGCTTCTCTTTTGGATACGTTGCTTCATGATACGTGGTTTGTGGTTGCTCATTTT GTTACTGGTATTATGCTTTCTGCTTCTCTTTTGGATACGTTGCTTCATGATACGTGGTTTGTGGTTGCTCATTTT GTTACTGGTATTATGCTTTCTGCTTCTCTTTTGGATACTTTGCTTCATGATACATGGTTTGTGGTTGCTCATTTT GTTACTGGTATTATGCTTTCTGCTTCTCTTTTGGATACGTTGCTTCATGATACGTGGTTTGTGGTTGCTCATTTT GTTACTGGTATTATGCTTTCTGCTTCTCTTTTGGATACGTTGCTTCATGATACGTGGTTTGTGGTTGCTCATTTT GTTACTGGTATTATGCTTTCTGCTTCTCTTTTGGATACGTTGCTTCATGATACGTGGTTTGTGGTTGCTCATTTT GTTACTGGTATTATGCTTTCTGCTTCTATTTTGGATACTTTGTTGCATGATACTTGGTTTGTTGTGGCTCATTTC GTTACTGGTATTATGCTTTCTGCTTCTATTTTGGATACTTTGTTGCATGATACTTGGTTTGTTGTGGCTCATTTC GGGATAGTTCTTTCTGCTTCTGTAATTGATGCCTTGTTTCATGATACTTGGTTTGTTATTGCTCATTTTCATTAT GGGATAGTTCTTTCTGCTTCTGTAATTGATGCCTTGTTTCATGATACTTGGTTTGTTATTGCTCATTTTCATTAT GGGATAGTTCTTTCTGCTTCTGTAATTGATGCCTTGTTTCATGATACTTGGTTTGTTATTGCTCATTTTCATTAT GGGATAGTTCTTTCTGCTTCTGTAATTGATGCCTTGTTTCATGATACTTGGTTTGTTATTGCTCATTTTCATTAT GGTATAGTTCTTTCTGCTTCGGTTATAGATGCTTTATTTCATGATACTTGATTTGTTATAGCTCATTTTCATTAT GGTATAGTTCTTTCTGCTTCGGTTATAGATGCTTTATTTCATGATACTTGATTTGTTATAGCTCATTTTCATTAT GGGATAGTTCTTTCTGCCTCTGTGATTGATGCTCTTTTTCATGATACATGATTCGTTATTGCCCACTTTCATTAT GATTATTTTGTCTTCTTCTATTTTGGATAGTCTGTTACATGATACCTGGTTTGTTGTTGCTCACTTTCACTATGT AATTATCTTGTCTTCTTCTATTTTGGACAGTTTATTGCATGACACTTGGTTTGTTGTTGCACATTTCCATTATGT CATCATTCTGTCCTCCTCCATACTGGATAGTCTGTTGCATGATACGTGGTTCGTCGTGGCTCATTTTCATTACGT CATCATTCTGTCCTCCTCCATACTGGATAGTCTGTTGCATGATACGTGGTTCGTCGTGGCTCATTTTCATTACGT ACTGGGATTATGTTGTCTGCTTCTATATTGGATACTTTGTTGCATGATACTTGGTTTGTGGTGGCCCATTTTCAT ATTGTGTTGTCTTCTTCTATAATGGATACTTTGTTGCATGACACTTGATTTGTAATAGCTCACTTTCATTATGTT ATTGTGTTGTCTTCTTCTATAATGGATACTTTGTTGCATGACACTTGATTTGTAATAGCTCACTTTCATTATGTT ATTGTGTTGTCTTCTTCTATAATGGATACTTTGTTGCATGACACTTGATTTGTAATAGCTCATTTTCATTATGTT GTAGTGCTTTCGTCTTCTGTGATGGATACTTTGATGCACGATACTTGATTTGTTATTGCTCATTTTCATTACGTT : : : : : : : : : : : : : : : : : : : : : : : : 1125 1125 1125 1125 1125 1125 1125 1125 1125 1125 1125 1125 1125 1125 1125 1124 1121 1124 1124 1125 1125 1125 1125 1125 FspNB-VN-c FspCB-VN-c Fhep-AU-co FspGHL-CNFgig-CN-co Fgig-ID-co Fbus(human Fbus(swine Csin-CN-co Csin-KR-co Csin-RU-co Csin-VN-co Oviv-TH-co Oviv-CN-co Ofel-RU-co Phet-VN-co Pohi-JP-co Pwes(2n)-K Pwes(3n)KR Ejap-VN-co Htai-KR-co Htai-LA-co Htai-VN-co Sfal-VN-co : : : : : : : : : : : : : : : : : : : : : : : : * 1140 * 1160 * 1180 * 1200 CATTATGTTCTTTCTTTAGGTTCTTATAGTAGTGTTGTTATCTCTTTTATTTGGTGGTGGCCTATTGTTACGGGT CATTATGTTCTTTCTTTAGGTTCTTATAGTAGTGTTGTTATCTCTTTTATTTGGTGGTGGCCTATTGTTACGGGT CATTATGTTCTTTCTTTAGGGTCTTATAGAAGTGTTGTTATCTCTTTTATTTGGTGGTGGCCTGTTGTGACTGGT CATTATGTTCTTTCTTTAGGTTCTTATAGTAGTGTTGTTATCTCTTTTATTTGGTGGTGGCCTATTGTTACGGGT CATTATGTTCTTTCTTTAGGTTCTTATAGTAGTGTTGTTATCTCTTTTATTTGGTGGTGGCCTATTGTTACGGGT CATTATGTTCTTTCTTTAGGTTCTTATAGTAGTGTTGTTATCTCTTTTATTTGGTGGTGGCCTATTGTTACGGGT CATTATGTTCTTTCATTAGGTTCTTATAGGAGTGTTGTTATATCTTTTATTTGATGGTGGCCAATAATTACTGAT CATTATGTTCTTTCATTAGGTTCTTATAGGAGTGTTGTTATATCTTTTATTTGATGGTGGCCAATAATTACTGAT GTTTTATCCCTAGGGTCGTTTAGGACGGTTGTTATTTCTGTAATTTGGTGATGACCTGTGTTTACGGGGTATAGC GTTTTATCCCTAGGGTCGTTTAGGACGGTTGTTATTTCTGTAATTTGGTGATGACCTGTGTTTACGGGGTATAGC GTTTTATCCCTAGGGTCGTTTAGGACGGTTGTTATTTCTGTAATTTGGTGATGACCTGTGTTTACGGGGTATAGC GTTTTATCTTTAGGGTCGTTTAGGACGGTTGTTATTTCTGTAATTTGGTGATGACCTGTGTTTACGGGGTATAGC GTGCTGTCATTAGGATCCTTTAGAACGGTGGTTATATCCGTTGTTTGGTGGTGGCCTGTTTTTACTGGTTACAGT GTGCTGTCATTAGGATCCTTTAGAACGGTGGTTATATCCGTTGTTTGGTGGTGGCCTGTTTTTACTGGTTATAGT GTTTTATCCTTGGGATCTTTTAGTACTGTTGTTATATCAATGATTTGGTGGTGGCCTGTGTTTACCGGGTTTAGC TCTATCTTTGGGATCTTATAGAACTGTGGTGATATCCCTCTTGTGATGATGGCCGGTTGTCACCGGGTTTAGCTT TTTATCCCTGGGATCTTATAGCACTGTTGTAATATCCCTTCTGTGATGGTGGCCTATTGTGACCGGGTTTAGTTT CTTGTCTCTGGGGTCCTACAGGACCGTAGTGATATCCCTGTTGTGGTGGTGGCCCGTTGTTACGGGGTTCAGTTT CTTGTCTCTGGGGTCCTACAGGACCGTAGTGATATCCCTGTTGTGGTGGTGGCCCGTTGTTACGGGGTTCAGTTT TATGTTTTATCCTTGGGTTCATATAGTGGTGTTGTGATTTCGTTTTTGTGGTGGTGGCCTATTATAACGGGGTAT TTATCGTTAGGTTCTTATAGGTCGGTGGTGATATCTATGATTTGATGATGGCCTGTGATAGTGGGTTATAGGTTG TTATCGTTAGGTTCTTATAGGTCGGTGGTGATATCTATGATTTGATGATGGCCTGTGATAGTGGGTTATAGGTTG ttatcgTTAGGTTCTTATATGTCgGTGGTGATATCTATGATTTGATGATGGCCTGTGATAGTGGGTTATAGGTTG TTGTCTTTAGGATCGTACAGTACTGTTGTAATCTCGTTGATCTGGTGGTGGCCTGCAGTTGTTGGGTCGAGACTT : : : : : : : : : : : : : : : : : : : : : : : : 1200 1200 1200 1200 1200 1200 1200 1200 1200 1200 1200 1200 1200 1200 1200 1199 1196 1199 1199 1200 1200 1200 1200 1200 FspNB-VN-c FspCB-VN-c Fhep-AU-co FspGHL-CNFgig-CN-co Fgig-ID-co Fbus(human Fbus(swine Csin-CN-co Csin-KR-co Csin-RU-co Csin-VN-co Oviv-TH-co Oviv-CN-co Ofel-RU-co Phet-VN-co Pohi-JP-co : : : : : : : : : : : : : : : : : * 1220 * 1240 * 1260 * TATAGGTTGAATCTTTTTATGTTGCAGGGGCATTGGTTTGTTTCCATGGTTGGGTTTAATATGTGTTTTTTCCCT TATAGGTTGAATCTTTTTATGTTGCAGGGGCATTGGTTTGTTTCCATGGTTGGGTTTAATATGTGTTTTTTCCCT TATAGTTTGAATCTTTTCATGTTGCAAGGTCATTGGGTTGTTTCTATGATTGGGTTTAATATGTGTTTTTTCCCT TATAGGTTGAATCTTTTTATGTTGCAGGGGCATTGGTTTGTTTCCATGGTTGGGTTTAATATGTGTTTTTTCCCT TACAGGTTGAATCTTTTTATGTTGCAGGGGCATTGGTTTGTTTCCATGGTTGGGTTTAATATGTGTTTTTTCCCT TATAGGTTGAATCTTTTTATGTTGCAGGGGCATTGGTTTGTTTCCATGGTTGGGTTTAATATGTGTTTTTTCCCT TATAGTTTAAAAGTTTCTTTGTTGCAAGGTCATTGGATTGTTTCTATGTTTGGTTTTAATTTGTGTTTTTTTCCG TATAGTTTAAAAGTTTCTTTGTTGCAAGGTCATTGGATTGTTTCTATGTTTGGTTTTAATTTGTGTTTTTTTCCG TTAAATAAGTATCTATTGATGGCTCATTGAGGTGTATCGATGACGGGGTTTAATCTCTGTTTTTTTCCTATGCAT TTAAATAAGTATCTATTGATGGCTCATTGAGGTGTATCGATGACGGGGTTTAATCTCTGTTTTTTTCCTATGCAT TTAAATAAGTATCTATTGATGGCTCATTGAGGTGTATCGATGACGGGGTTTAATCTCTGTTTTTTTCCTATGCAT TTAAATAAGTATCTATTGATGGCTCATTGAGGTGTATCGATGACGGGGTTTAATCTCTGTTTTTTTCCTATGCAT TTAAATAAGTATTTATTAATGGCTCATTGAGGTGTCTCAATGTTAGGGTTCAATCTGTGTTTCTTTCCAATGCAC TTAAATAAGTATTTATTAATGGCTCATTGAGGTGTCTCAATGTTAGGGTTCAATCTATGTTTCTTTCCAATGCAC CTTAACAAGTATTTGTTAGTGGCTCATTGAGGTGTGTCAATGATAGGGTTTAATCTTTGTTTCTTCCCAATGCAT GAATAAGTATTTGTTGCAGGGTCATTGGGTGGCATCTATGGTGGGGTTTAATCTGTGTTTTTTTCCCATGCATTA AAATAAGTATTTGTTGCAAGGGCATTGGGTGGCATCAATGGTAGGGTTTAATTTTTGTTTTTTCCCCATGCATTA : : : : : : : : : : : : : : : : : 1275 1275 1275 1275 1275 1275 1275 1275 1275 1275 1275 1275 1275 1275 1275 1274 1271 70 Pwes(2n)-K Pwes(3n)KR Ejap-VN-co Htai-KR-co Htai-LA-co Htai-VN-co Sfal-VN-co : : : : : : : GAATAAGTACCTGTTGCAGGGTCATTGGGTGAGGTCGATGCTGGGGTTTAACCTTTGTTTTTTTCCGATGCATTA GAATAAGTACCTGTTGCAGGGTCATTGGGTGAGGTCGATGCTGGGGTTTAACCTTTGTTTTTTTCCGATGCATTA AGGTTAAATCGTTTATTATTGTGGGGACATTGAATTGTTTCGATGATAGGGTTTAATTTGTGTTTTTTCCCTATG AAAAAGTACTTGTTGATGGCTCATTGGGGGGTGTCAATGGTTGGATTCAATTTGTGTTTTTTTCCTATGCATTTT AAAAAGTACTTGTTGATGGCTCATTGGGGGGTGTCAATGGTTGGATTCAATTTGTGTTTTTTTCCTATGCATTTT AAAAAGTACTTGTTGATGGCTCATTGGGGGGTGTCAATGGTTGGATTTAATTTGTGTTTTTTTCCTATGCATTTT AAAAAGTATTTATTGATGGGCCATTGGGGTGTGTCGATGATAGGTTTTAACTTGTGCTTTTTCCCGATGCACTTT : : : : : : : 1274 1274 1275 1275 1275 1275 1275 FspNB-VN-c FspCB-VN-c Fhep-AU-co FspGHL-CNFgig-CN-co Fgig-ID-co Fbus(human Fbus(swine Csin-CN-co Csin-KR-co Csin-RU-co Csin-VN-co Oviv-TH-co Oviv-CN-co Ofel-RU-co Phet-VN-co Pohi-JP-co Pwes(2n)-K Pwes(3n)KR Ejap-VN-co Htai-KR-co Htai-LA-co Htai-VN-co Sfal-VN-co : : : : : : : : : : : : : : : : : : : : : : : : 1280 * 1300 * 1320 * 1340 * ATGCATTATCTTGGTATGTGTGGTTTGCCGCGTCGTGTTTGTGTTTATGATCCTGATTTTTATTGGCTTAGTGTT ATGCATTATCTTGGTATGTGTGGTTTGCCGCGTCGTGTTTGTGTTTATGATCCTGATTTTTATTGGCTTAGTGTT ATGCACTACCTTGGTATGGCGGGGTTGCCTCGCCGGGTTTGTGTTTATGATCCGGATTTTTATTGGCTTAGTGTT ATGCATTATCTTGGTATGTGTGGTTTGCCGCGTCGTGTTTGTGTTTATGATCCTGATTTTTATTGGCTTAGTGTT ATGCATTATCTTGGTATGTGTGGTTTGCCGCGTCGTGTTTGTGTTTATGATCCTGATTTTTATTGGCTTAGTGTT ATGCATTATCTTGGTATGTGTGGTTTGCCGCGTCGTGTTTGTGTTTATGATCCTGATTTTTATTGGCTTAGTGTT ATGCATTATTTGGGTATGTCTGGGCTTCCTCGCCGGGTGTGTGTTTATGATCCTGATTTTTATTGACTTAATGTG ATGCATTATTTGGGTATGTCTGGGCTTCCTCGCCGGGTGTGTGTTTATGATCCTGATTTTTATTGACTTAATGTG TTTTTAGGTGTTTATGGGCTTCCACGTCGGGTGTGTTTGTATGAGCCCTGCCTTCAGTGGTTGAATACTTTGTGT TTTTTAGGTGTTTATGGGCTTCCACGTCGGGTGTGTTTGTATGAGCCCTGCCTTCAGTGGTTGAATACTTTGTGT TTTTTAGGTGTTTATGGGCTTCCACGTCGGGTGTGTTCGTATGAGCCTTGCTTTCAGTGGTTGAATACTTTGTGT TTTTTAGGTGTTTATGGGCTTCCACGTCGGGTGTGTTCGTATGAGCCCTGCTTTCAGTGGTTGAATACTTTGTGT TTTTTAGGTATTTACGGTCTTCCACGTCGTGTGTGTTCTTATGAGCCTTGTTTTCAGTGGGTGAATACTGTGTGT TTTTTAGGTATTTACGGTCTTCCACGTCGCGTGTGTTCTTACGAGCCTTGTTTTCAGTGGGTGAATACTGTGTGT TTTTTAGGCGTTTATGGACTACCGCGTCGGGTGTGTTCATATGAGCCTTGTTTTCAGTGGATAAATACTTTGTGT CCTTGGGGCTTATGGTCTTCCTCGGCGGGTGTGTAACTATGAGCCTGCTTTTCAGTGGCTAAATAATGTTTCTTC TCTTGGGGCTTATGGTCTTCCACGTCGGGTGTGTAACTATGAGCCGGCCTTTCAGTGATTAAACAATATTTCTTC CCTCGGAGCTTACGGTCTCCCACGTCGGGTGTGTAACTATGAGCCCGCCTTTCAGTGGTTGAACAAAATCTCCTC CCTCGGAGCTTACGGTCTCCCACGTCGGGTGTGTAACTATGAGCCCGCCTTTCAGTGGTTGAACAAAATCTCCTC CATTATTTTGGGTTGCAGGGGTTACCACGTCGTGTTTGTGTTTTTGATCCTGAGTTTTGTTGGTTGAATTTTATT TTAGGGTTTCAGGGTTTACCTCGTCGGGTGTGTGTTTATGAGCCTTGTTTTCAGTGGTTGAAAACTATGGCTAGG TTAGGGTTTCAGGGTTTACCTCGTCGGGTGTGTGTTTATGAGCCTTGTTTTCAGTGGTTGAAAACTATGGCTAGG TTAGGGTTTCAGGGTTTACCTCGTCGGGTGTGTGTTTATGAGCCTTGTTTTCAGTGGTTGAAAACTATGGCTAGG TTAGGGTTGCATGGTCTTCCTCGTCGGGTGTGTGTTTATGAGCCGGCCTTTCAGTGGTTGAATAGTATATCAACT : : : : : : : : : : : : : : : : : : : : : : : : 1350 1350 1350 1350 1350 1350 1350 1350 1350 1350 1350 1350 1350 1350 1350 1349 1346 1349 1349 1350 1350 1350 1350 1350 FspNB-VN-c FspCB-VN-c Fhep-AU-co FspGHL-CNFgig-CN-co Fgig-ID-co Fbus(human Fbus(swine Csin-CN-co Csin-KR-co Csin-RU-co Csin-VN-co Oviv-TH-co Oviv-CN-co Ofel-RU-co Phet-VN-co Pohi-JP-co Pwes(2n)-K Pwes(3n)KR Ejap-VN-co Htai-KR-co Htai-LA-co Htai-VN-co Sfal-VN-co : : : : : : : : : : : : : : : : : : : : : : : : 1360 * 1380 * 1400 * 1420 GTTTCTAGAGTAGGTGCTTTTATTTCTGCTGGTAGTGCTTTTTTGTTTTTGTTTGTTTTGTGGGAGTCTTTTGTT GTTTCTAGAGTAGGTGCTTTTATTTCTGCTGGTAGTGCTTTTTTGTTTTTGTTTGTTTTGTGGGAGTCTTTTGTT GTTTCTAGTTTAGGTGCTCTTGTCTCGGCAGGTAGTGCCTTTTTGTTTGTGTTTATTTTGTGGGAGTCCTTTGTT GTTTCTAGAGTAGGTGCTTTTATTTCTGCTGGTAGTGCTTTTTTGTTTTTGTTTGTTTTGTGGGAGTCTTTTGTT GTTTCTAGAGTAGGTGCTTTTATTTCTGCTGGTAGTGCTTTTTTGTTTTTGTTTGTTTTGTGGGAGTCTTTTGTT GTTTCTAGAGTAGGTGCTTTTATTTCTGCTGGTAGTGCTTTTTTGTTTTTGTTTGTTTTGTGGGAGTCTTTTGTT TTATCAAGTTTAGGGGGTGTGGTATCAACTGTAAGGGCCTTTTTTCTTTTTTTTGTTTTATGGGAGTCTTGTGTG TTATCAAGTTTAGGGGGTGTGGTATCAACTGTAAGGGCCTTTTTTCTTTTTTTTGTTTTATGGGAGTCTTGTGTG AGGATGGGGGCGATTTTTTCGGTTTTCAGTGGGTTTACGCTTATGTATATTGTTTGAGAATCTATGGCTGTTTGT AGGATGGGGGCGATTTTTTCGGTTTTCAGTGGGTTTACGCTTATGTATATTGTTTGAGAATCTATGGCTGTTTGT AGGATGGGGGCGATTTTTTCGGTTTTCAGTGGGTTTACGCTTATGTATATTGTTTGAGAATCTATGGCTGTTTGT AGGATGGGGGCGATTTTTTCGGTTTTCAGTGGGTTTACGCTTATGTATATTGTTTGAGAATCTATGGCTGTTTGT AGAGTTGGTGGTATCTTTTCTGTGTTTAGTGGTTTTACCCTGATATACGTTATTTGGGAGTCTATGGCGGTGCGT AGAGTTGGTGGTATCTTTTCTGTGTTTAGTGGTTTTACCCTGATATACGTTATTTGGGAGTCTATGGCGGTGCGT AGTATAGGTGGGATATTCTCGGTTTTTAGGGGGTTTACTCTTATGTATATAGTTTGGGAGTCGATAGCTGTGAGT TGTTGGTGCTTTAGTTTCTGTGGTTAGGGCCTTTTTTTTGGTTTTTATTCTATGGGAGTCTTTGGTTGTGGGTAA TGTTGGAGCTTTAATTTCTGTGGTTAGGGCTTTTTTTTTGGTTTTTATTCTTTGAGAGTCTCTTGTGGTTGGAAA TGTGGGGGCGCTGGTTTCTGTTGTCAGTGCTTTTTTTTTGGTTTTTATCCTTTGGGAGTCTCTCGTTGTTGGGAA TGTGGGGGCGCTGGTTTCTGTTGTCAGTGCTTTTTTTTTGGTTTTTATCCTTTGGGAGTCTCTCGTTGTTGGGAA TGTAGGTTGGGCGGTATTCTTTCTGCAAGGAGTGCTTTTTTtTTTGTATATATTTTGTGGGAGTCTTATACTGTG TTGGGGGGACTTCTTTCTGTTTTTAGGGGGTTTATGTTAGCTTTCATTTTATGAGAGTCGTTGTCTGTGGGTAAT TTGGGGGGACTTCTTTCTGTTTTTAGGGGGTTTATGTTAGCTTTCATTTTATGAGAGTCGTTGTCTGTGGGTAAT CTGGGGGGACTTCTTTCTGTTTTTAGGGGGTTTATGTTAGCTTTCATTTTATGAGAGTCGTTGTCTGTGGGTAAT ATGGGGGGTCTGATTTCTGTGTTCAGCGGATTTGTTTTGTTGTATATACTCTGGGAGTCGTTGGTTGTGGGTAAT : : : : : : : : : : : : : : : : : : : : : : : : 1425 1425 1425 1425 1425 1425 1425 1425 1425 1425 1425 1425 1425 1425 1425 1424 1421 1424 1424 1425 1425 1425 1425 1425 FspNB-VN-c FspCB-VN-c Fhep-AU-co FspGHL-CNFgig-CN-co Fgig-ID-co Fbus(human Fbus(swine Csin-CN-co Csin-KR-co Csin-RU-co Csin-VN-co Oviv-TH-co Oviv-CN-co Ofel-RU-co Phet-VN-co Pohi-JP-co Pwes(2n)-K Pwes(3n)KR Ejap-VN-co Htai-KR-co Htai-LA-co Htai-VN-co Sfal-VN-co : : : : : : : : : : : : : : : : : : : : : : : : * 1440 * 1460 * 1480 * 1500 GTTGGTAATCGTGTTGTTTCGTCTTGAGGTAACAGTTCGTTAGTGTTGAATGTTATTACTTTACCTGGCCCTCAG GTTGGTAATTGTGTTGTTTCGTCTTGAGGTAGCAGTTCGTTAGTATTGAATGTTATTACTTTGCCTGGCCCTCAG GTAGGTAATTGTGTTGTCTCTTCTTGAGGTAGCAGGTCTTTAGTGTTGAATGTTATTACTTTGCCAGGTCCTCAA GTTGGTAATTGTGTTGTTTCGTCTTGAGGTAGCAGTTCGTTAGTATTGAATGTTATTACTTTGCCTGGCCCTCAG GTCGGTAATCGTGTTGTTTCGTCTTGAGGTAACAGTTCGTTAGTGTTGAATGTTATTACTTTACCTGGCCCTCAG GTTGGTAATCGTGTTGTTTCGTCTTGAGGTAATAGTTCGTTAGTGTTGAATGTTATTACTTTACCTGGCCCTCAG ATAGGTAATCGGGTACTTTCTGTTTGGGGTTCCGGTTCTTTGATTTTGAATGTTGTTACTTTACCTAGACCTCAG ATAGGTAATCGGGTACTTTCTGTTTGGGGTTCCGGTTCTTTGATTTTGAATGTTGTTACTTTACCTAGACCTCAG AACCGTGTCATTAATCTGTGGGGCGGGGCTCATGTTGTTTTTAATGTTTTGGTGAATCCGATTGGGGCGCATAGT AACCGTGTCATTAATCTGTGGGGCGGGGCTCATGTTGTTTTTAATGTTTTGGTGAATCCGATTGGGGCGCATAGT AACCGTGTCATTAATCTGTGGGGCGGGGCTCATGTTGTTTTTAATGTTTTGGTGAATCCGATTGGGGCACATAGT AACCGTGTCATTAATCTGTGGGGCGGGGCTCATGTTGTTTTTAATGTTTTGGTGAATCCGATTGGGGCGCATAGT AATCGTGTAGTTAAATTGTGAGGGGGGGCTCATGTTGTGTTTAATGTGTTAGTTAATCCCGTTGGGATGCATAGC AATCGTGTAGTTAAATTGTGAGGGGGGGCTCATGTTGTGTTTAATGTGTTAGTTAATCCCGTTGGGATGCATAGC AATCGTGTAGTTCATTTGTGGGGTGGAGCCCATGTTGTGTTTAACGTTTTGGTTAATCCAATTGGGGCACATAGT TCGTGTTGTTGGTGTTTGGGGTACTAAAGCCCTGGTTATGAATGCCTTAGTTACTCCAGTTCCCCATCACAGTGT TCGTGTTGTTGGGGTTTGAGGTAGCAAATCTTTGGTTATGAATGCTTTGGTTACTCCTGTGCCTCATCATAGGGT TCGTGTCGTGGGTATCTGGGGTACGAATGCTTTAGTGGTGAATGCGTTGGTGACGCCTGTGCCGCATCACAGGAT TCGTGTCGTGGGTATCTGGGGTACGAATGCTTTAGTGGTGAATGCGTTGGTGACGCCTGTGCCGCATCACAGGAT AAGAAAGTGGTAGTGTCTTGTTGGGGTAGAAATTCTTTGGTGCTTAAAGTAGTTACTTTACCTCTACCACATCAT CGGGTGATAGGGTTATGGGGTAGTAAAGCCTTGGTTTTAAAAGTAGTAACTTCTCCTGTVCCGCATCATTGTAGT CGGGTGATAGGGTTATGGGGTAGTAAAGCCTTGGTTTTAAAAGTAGTAACTTCTCCTGTVCCGCATCATTGTAGT CGGGTGATAGGGTTATGGGGTAGTAAAGCCTTGGTTTTAAAAGTAGTAACTTCTCCTGTGCCGCATCATTGTAGT CGGGTTATTGGGCTATGGGGAAGTAATGGGTTGATTTTAAATGTTGTTACCCTGCCAGTACCCCATCATTTTAGT : : : : : : : : : : : : : : : : : : : : : : : : 1500 1500 1500 1500 1500 1500 1500 1500 1500 1500 1500 1500 1500 1500 1500 1499 1496 1499 1499 1500 1500 1500 1500 1500 71 FspNB-VN-c FspCB-VN-c Fhep-AU-co FspGHL-CNFgig-CN-co Fgig-ID-co Fbus(human Fbus(swine Csin-CN-co Csin-KR-co Csin-RU-co Csin-VN-co Oviv-TH-co Oviv-CN-co Ofel-RU-co Phet-VN-co Pohi-JP-co Pwes(2n)-K Pwes(3n)KR Ejap-VN-co Htai-KR-co Htai-LA-co Htai-VN-co Sfal-VN-co : : : : : : : : : : : : : : : : : : : : : : : : * 1520 * 1540 * 1560 CATAATAGTTATATGAATGGTGCGGGTCGTTGAGTTTTTTAG CATAATAGTTATATGAATGGTGCGGGTCGTTGAGTTTTTTAG CATAATAGTTATATGAATGGTGTTGGTCGCTGGGTTTTTTAG CATAATAGTTATATGAATGGTGCGGGTCGTTGAGTTTTTTAG CATAATAGTTATATGAATGGTGCGGGTCGTTGAGTTTTTTAG CATAATAGTTATATGAATGGTGCGGGTCGTTGAGTTTTTTAG CATAAAGGTTATATGACTGGGGGTTGTCGGTGGTGTGCTTAG CATAAAGGTTATATGACTGGGGGTTGTCGGTGGTGTGCTTAG CCTTATCTTCATTATCCTGTTTGTTGAGTTCCGAGTGAGATTCGACGGTTGGGGGTGTAA CCTTATCTTCATTATCCTGTTTGTTGAGTTCCGAGTGAGATTCGACGGTTGGGGGTGTAA CCTTATCTTCATTATCCTGTTTGTTGAGTTCCGAGTGAGATTCGACGGTTGGGGGTGTAA CCTTATCTTCATTATCCTGTTTGTTGAGTTCCGAGTGAGATTCGACGGTTGGGGGTGTAA TCTTACTTATATTATCCTGTTTGTTGACTTCCCGCCGGGGGCCGTGTTTAG -TCTTACTTATATTATCCTGTTTGTTGACTTCCCGCCGGGGGCCGTGTTTAG -TCTTATTTACATTATCCCATTTGTTGGATGCCTAGAGGGGGCCGGCGATTGGAGGGGGTGTAG TTATATCAGTGGGCCGTCTCGTTGGCTTTCTTTTTAG -TTACATTAGTGGGCCGTCTCGTTGGCTTTCTTTTTAG -TTACATCAGTGGGCCGTCGCGGTGGCTTTCTTTTTAG -TTACATCAGTGGGCCGTCGCGGTGGCTTTCTTTTTAG -GGGGATTATATTCATACTTCAATACATTGGGTGTTTTAG -TATATGAGTGAGCCGGTAACGTGGTTTTATCGTTGGTATTAG TATATGAGTGAGCCGGTAACGTGGTTTTATCGTTGGTATTAG TATATGAGTGAGCCGGTAACGTGGTTTTATCGTTGGTATTAG TATGTGGGTGAGCCGTGCACTTGGCTTAAGGGTGTGTCGGTTTAG : : : : : : : : : : : : : : : : : : : : : : : : 1542 1542 1542 1542 1542 1542 1542 1542 1560 1560 1560 1560 1551 1551 1563 1536 1533 1536 1536 1539 1542 1542 1542 1545 ... ÁNH NGHIÊN CỨU BIẾN ĐỔI THÀNH PHẦN GEN NHÂN ITS-2, 28S rRNA VÀ GEN TY THỂ COX1 CỦA LOÀI SÁN LÁ GAN LỚN FASCIOLA SSP DẠNG THUẦN VÀ DẠNG LAI GÂY BỆNH TRÊN ĐỘNG VẬT TẠI VIỆT NAM TĨM TẮT LUẬN VĂN THẠC... COX1 loài sán gan lớn Fasciola sp dạng dạng lai gây bệnh động vật Việt Nam Mục tiêu đề tài - Giải trình tự phân tích gen nhân ITS-2, gen 28S sán gan lớn dạng xác định dạng laigây bệnh động vậttại... biệt loài Fasciola thực phương pháp sinh học phân tử dựa thị gen nhân ty thể 2 Xuất phát từ yêu cầu đó, tiến hành thực đề tài: Nghiên cứu biến đổi thành phần gen nhân ITS-2, 28S rRNA gen ty thể