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Chapter 017 Diagnosing Infections Multiple Choice Questions Which of the following is not a phenotypic method of identification? A Morpholo gy B Gram stain reaction C Acid-fast reaction D Antibody response E Endospore s Biochemical tests include all of the following except A Gram stain reaction B Acid-fast reaction C Cell wall composition D Sugar fermentation E Gas production The primary advantage of genotypic methods of identification is A It is easier than any other method B It is less expensive than other methods C It is widely available D Culturing of the organism is not required E All of the choices are correct Specimen collection A Is always done by a medical professional B Must be done under sterile conditions C Must utilize aseptic techniques D Does not require special handling E All of the choices are correct Each of the following can be used for specimen collection except A Saliv a B Ski n C Spinal fluid D Hai r E Throa t Which sample is not collected by sterile needle aspiration? A Bloo d B Urin e C Cerebrospinal fluid D Tissue fluids E All are collected by sterile needle aspiration Direct antigen testing differs from direct fluorescence testing in that the results A Are read with the naked eye B Require specialized equipment C Are more accurate D Are less accurate E Require an electron microscope Uri ne an d fe cal sp eci m en s re qu ire A B C D E Ph ag e ty pi ng is us ef ul in id en tif yi ng A B C D E 10 An im als ar e re qu ire d for th e cul tiv ati on of A B C D E 56 ELI SA te sts us e a flu or es ce nt dy e as a la be l to tra ce an tig en an tib od y re ac tio ns TR UE Lea rnin g Obj ecti ve: 17 13 List the ste ps of an ELIS A and expl ain the dif ere nce bet wee na dire ct and an indi rect test 57 Th e tu be rc uli n te st is an ex a m pl e of an in vit ro se rol og ica l te st FA LS E Lea rnin g Obj ecti ve: 17 03 Expl ain the idea s beh ind pre sum ptiv e vs frm ator y dat a 58 Air or flu id sa m pl es ca n be m on ito re d thr ou gh bi os en so rin g TR UE Lea rnin g Obj ecti ve: 17 07 Pro vide an expl ana tion for how PCR is usef ul for infe ctio us dise ase dia gno sis 59 Th e po ly m er as e ch n re ac tio n re qu ire s en nu cle as e to cu t sa m pl es of D NA FA LS E Lea rnin g Obj ecti ve: 17 07 Pro vide an expl ana tion for how PCR is usef ul for infe ctio us dise ase dia gno sis Chapter 017 Diagnosing Infections Summary Ca te go ry # of Qu est ion s Le ar ni ng Ob je cti ve :1 01 Na m et he thr ee m aj or ca te go rie s of mi cr ob ei de nti fic ati on te ch ni qu es Le ar ni ng Ob je cti ve :1 02 Id en tif ys o m ei m po rta nt co nsi de rat io ns ab ou tc oll ec tin g sa m pl es fro m pa tie nt sf or mi cr ob ial id en tifi ca tio n Le ar ni ng Ob je cti ve :1 03 Ex pl nt he id ea s be hi nd pr es u m pti ve vs co nfi rm at or y da ta Le ar ni ng Ob je cti ve :1 04 Ex pl nt he id ea s be hi nd pr es u m pti ve vs co nfi rm at or y da ta Le ar ni ng Ob je cti ve :1 04 Lis ta tl ea st di ffe re nt te st st tf all in th e dir ec ti de nti fic ati on ca te go ry Le ar ni ng Ob je cti ve :1 05 Ex pl nt he m n pri nc ipl e be hi nd bi oc he mi ca lt es ts Le ar ni ng Ob je cti ve :1 06 Lis tt he m aj or st ep si n a hy bri di za tio n m et ho d of mi cr ob ei de nti fic ati on Le ar ni ng Ob je cti ve :1 07 Pr ov id e an ex pl an ati on for ho w PC Ri s us ef ul for inf ec tio us di se as e di ag no sis Le ar ni ng Ob je cti ve :1 08 Gi ve at ho ro ug h de fin iti on of th et er m se rol og y Le ar ni ng Ob je cti ve :1 09 Di ffe re nti at e be tw ee n se nsi tiv ity an d sp ec ifi cit y Le ar ni ng Ob je cti ve :1 10 Di ffe re nti at e be tw ee n se nsi tiv ity an d sp ec ifi cit y Le ar ni ng Ob je cti ve :1 10 Di sc us st he co nc ep ts of ag gl uti na tio n an d pr ec ipi tat io n an d w he n ea ch is ap pr op ria te Le ar ni ng Ob je cti ve :1 10 Di sc us st he co nc ep ts of ag gl uti na tio n an d pr ec ipi tat io n an d w he n ea ch is ap pr op ria te Le ar ni ng Ob je cti ve :1 11 Lis tt he st ep s of a W es ter n bl ot Le ar ni ng Ob je cti ve :1 12 De sc rib e ho w co m pl e m en t fi xa tio n wo rk s Le ar ni ng Ob je cti ve :1 13 Lis tt he st ep s of an EL IS A an d ex pl nt he di ffe re nc e be tw ee n a dir ec ta nd an in dir ec tt es t ... fluids E All are collected by sterile needle aspiration Direct antigen testing differs from direct fluorescence testing in that the results A Are read with the naked eye B Require specialized equipment... C Are more accurate D Are less accurate E Require an electron microscope Uri ne an d fe cal sp eci m en s re qu ire A B C D E Ph ag e ty pi ng is us ef ul in id en tif yi ng A B C D E 10 An... 10 An im als ar e re qu ire d for th e cul tiv ati on of A B C D E 11 W hic h te st is es pe cia lly go od for ba ct eri a th at ar e no t re ad ily cul tiv at ed in th e la b? A B C D E