Posters t6 FUNCTIONAL FOODS AND BIOACTIVE COMPOUNDS

Posters Ann Nutr Metab 2013;63(suppl 1): 1-1960 T6 Functional Foods and Bioactive Compounds PO2658 EVALUATION OF DEFATTED SOY BASED FORMULAS PREPARED FOR INFANTS AND YOUNG CHILDREN WITH ACUTE DIARRHEA S Zaki1, M Abou-Zekri2, A El-Akel1, L El-mahdi3, N Hassan4 Department of Food Technology,Faculty of Agriculture,Cairo University,Giza, Egypt Department of Endemic Diseases, Abou Elrish Children Hospital, Cairo university, Cairo, Egypt Department of Horticulture Crops, Food Technoloy Institute, Agriculture Research Center, Ministry of Agriculture,Giza, Egypt Department of Experimental Kitchen, Food Technology Institute, Agriculture Research Center, Ministry of Agriculture,Giza, Egypt Background and objectives: Recent data supported that diet composition introduced to infants and young children during diarrheal episodes is controversial so it has been the concern of extensive research Study aimed to formulate and evaluate some proposed formulas for this group during acute diarrhea Methods: Six different defatted soy based formulas with yellow carrots, rice, potato starch powder and orange were prepared Outpatients infants and young children (140) with acute diarrhea, visiting the Center for Social and Preventive Medicine, Cairo University Childrens Hospital, Egypt, were enrolled in the study Participants were randomly assigned to groups, 20 each The control group did not receive any dietetic intervention At the first visit, participant mother in the other groups randomly received a formula at in a powder form to be dissolved and used at home Patients were followed up for weeks in two visits (after and 15 days of enrollment) and assessed for diarrhea duration, number of motions/day, stool consistency, weight gain and their acceptability of the formulas Statistical analysis of the results was performed Results: Feeding patients formulas demonstrated insignificant (p< 0.05) differences among age, gender, and illness chaâ 2013 S Karger AG, Basel 0250-6807/13/636-0001$38.00/0 E-Mail karger@karger.com www.karger.com/anm Published online: September 13, 2013 racterize of cases The percentages of cases with watery, loose and semi solid stools were greatly reduced after five days in all the groups Insignificant difference (p< 0.05) was found among weights of formulas groups and control at enrollment, after and 15 days These groups showed significantly lower mean number of motions per day after getting proposed formulas for dayscompared to control group (p0.05) The minimum lipid droplet formation (79.9%) by Oil Red O staining was found under the following experimental conditions: P = 100.8 g/mL, C = 4.7 g/mL, and A = 89.2 g/ mL, respectively The maximum glycerol content (0.18 mM) was found under the conditions: P = 82.2 g/mL, C = 5.9 g/ mL, and A = 58.4 g/mL To validate the optimized formula, the activities were compared with the experimental values The optimal formula on lipid droplet formation was lower formation thanindividually treated P (100 g/mL), C (10 g/mL), and A (100 g/mL) The optimal formula on released glycerol was higher release than individually treated samples Conclusion: Our data demonstrate that the developed two formulas strongly inhibit the adipocyte differentiation Furthermore, these beverages might be used as a potential complementary treatment for the obese patients Key words: 3T3-L1 preadipocyte, adipocyte differentiation, response surface methodology, functional beverage Key words: Phytochemical, antioxidant, acetylcholinesterase inhibitor, pidada PO3006 OPTIMIZATION OF MIX CONDITION WITH PUMPKIN, CORN SILK, AND ADZUKI BEAN FOR INHIBITION OF ADIPOCYTE DIFFERENIATION IN 3T3-L1 CELL J HeePark1, R YoungKim2, E Park1 Department of Food and Nutrition Kyungnam Univ, 449 Woryeong-dong, Masanhappo-gu, Changwon, Gyeongnam, Republic of Korea Department of Hotel culinary and Bakery, Changsin College, Masanhoewon-gu, Changwon, Gyeongnam, Republic of Korea PO3007 THE IMPACT OF THE NATURAL PHYTOESTROGENIC DIETOTHERAPYON SEX HORMONE METABOLISM IN MENOPAUSAL WOMEN AND RISK OF BREAST CANCER A Kostrzewa Tarnowska1, M Czlapka Matysik1, M Fejfer1, J Mierkiewicz1, J Jeszka1 Department of Human Nutrition and Hygiene, Poznan University of Life Sciences, Poland Background and objectives: Obesity is important public concerns and there is increasing demand for effective therapeutic strategies Pumpkin, corn silk, and adzuki bean have been used as traditional remediesagainst obesity in Korea Therefore, the purpose of this study was to develop functional beverage using pumpkin (P), corn silk (C), and adzuki bean (A) with anti-obesity effect Method: The optimum formulation for mixing ingredients was achieved using response surface methodology (RSM), using a central composite design (CCD) A CCD was applied to investigate the mixing effects of independent variables, which included the P, C, and A, on dependent variables of glycerol and Oil Red O staining The latter variables are biomarker for predicting inhibitory effect on 3T3-L1 adipogenesis Results: These data obtained from the assays fitted well in the each model as the lack-of-fit statistic was non-significant Background and objectives:Phytoestrogens arenon-steroidal plant-derived compoundswith strong antioxidant properties.Phytoestrogensthrough binding to estrogen receptors can induce significant estrogenic properties and play a role in alleviating thesymptoms of menopause and prevention of hormone-dependentcancers The objective of this study was to investigate whether a diet rich in antioxidants would affect sex hormone metabolism and reduce the risk of breast cancer Methods: The study was conducted among 80 women in the perimenopausal period, who were characterized by an increased risk of hormone - dependent cancer The nutritionalintervention with phytoestrogens enrichedproducts took weeks The energy, nutritive value and level of antioxidants indaily food rations were analyzed using the Wikt Pro computer program Estrogen metabolites (2 OHE, 16-OHE1) were determined from 24-urine samples by ELISA method The EMR index was calculated Thetotal antioxidant capacity was measured Results: The nutritional intervention based on products enriched in phytoestrogens increases antioxidant capacity in human plasma (TAC) Moreover, the significant decrease of estrogen promoting the cancer (16-OHE1) and increase of the metabolite inhibiting carcinogenesis (2-OHE) were observed Abstracts Ann Nutr Metab 2013;63(suppl 1):11960 1711 Simultaneously the studyalso indicated a significant increase of estrogen index (EMR) Conclusions: It was concluded that the higher consumption of antioxidants, especially phytoestrogens, was correlated with higher total antioxidant capacity and a significant increase of the estrogen index which means reduction of the risk ofbreast cancer Key words:antioxidant capacity, phytoestrogens, hormonedependent cancer, menopausal women PO3383 BERRIES POLYPHENOLS: A MULTIAPPROACH TO DISCLOSE HUMAN HEALTH BENEFITS L Tavares1,2, J Carolina1, R C Pimpóo1, P Pinto1,3, D Macedo1, A Gomes1, I Figueira1, M Alves1, R B Ferreira1,4, C N Santos1,2 Instituto de Tecnologia Quớmica e Biolúgica, Universidade Nova de Lisboa, Av da Repỳblica, Oeiras, Portugal Instituto de Biologia Experimental e Tecnolúgica, Oeiras, Portugal Escola Superior Agrỏria, Instituto Politộcnico de Santarộm, Qta Galinheiro, Santarộm, Portugal Departamento de Botõnica e Engenharia Biolúgica, Instituto Superior de Agronomia, Universidade Tộcnica de Lisboa, Tapada da Ajuda, Lisboa, Portugal Berries are well recognized as polyphenols rich fruits, whose consumption bring benefits to human health However, the significance of its dietary intake is not well known in particular in countries that doesnt have tradition of harvesting/consuming those fruits In Portugal, a food frequency questionnaire highlighted that berries only represent 9% of fruit daily intake of Portuguese population, although assigning 17% of polyphenols daily ingested Although being a considerable quantity of ingested polyphenols, for compounds to be effective they must be bioaccessible Polyphenols after ingestion are extensively altered and their bioaccessible quantities limited Then, studies that intend to clarify the beneficial effects of berry polyphenols should access those alterations and/or take them into consideration An in vitro digestion model has been used to mimic gastrointestinal digestion, prior to test berries digested metabolites in in vitro cell models (from simple yeast to human cells) Berries tested in yeast strains ameliorate wild type response to oxidative stress and strawberry tree fruits, in particular, have the ability to rescue an oxidative-sensitive strain phenotype A modulation of cellular defences, such as the glutathione balance, rather than a direct reactive oxygen species 1712 Ann Nutr Metab 2013;63(suppl 1):11960 scavenging was verified Similar protective mechanisms are mediated by blackberries digested metabolites in human cells submitted to oxidative stress Those metabolites seem to exert a cytoprotective effect through the activation of preconditioning mechanisms, rather than direct antioxidant capacity Finally, human interventions studies have also been conducted in order to determine the bioavailable metabolites from berries juice, their kinetics and range of physiological concentrations in human serum Then, in future works bioavailable metabolites could be tested in cell models to study their mechanism of action on human body Acknowledgements: This work was supported by EU FP7-KBBE-2010-4265942 and FCT through PEst-OE/EQB/ LA0004/2011, PTDC/BIA-BCM/111617/2009 and grants Key words: berries polyphenols, in vitro digestion model, cell models, preconditioning mechanism, human intervention study PO3384 DEVELOPMENT OF A SWEET AND ENERGETIC BAR USING SESAME M E Sỏnchez-Pardo1, E Jimộnez-Garcớa1, A G TapiaLara1, M Jimộnez-Sỏnchez2, E Torres-Maravilla1, J Blancas-Nỏpoles1 Instituto Politecnico Nacional, Mộxico Instituto de Tecnologico de Estudios Superiores de Monterrey, Nuevo Leún, Mộxico Background and objectives: In Mexico, the sesame production is 1.3% and is used for preparing sweets, biscuits and confectionery in general The aim of this work was to elaborate confectionery products whose main ingredient was the sesame Methods: The products were: hard candy added sesame and sesame energetic bar The materials used were sesame, sucrose, citric acid, corn syrup, glucose, gelatin, water, and additives The sesame evaluation was made based on the norm: NMXFF-071-1994-SCFI Non-industrialized products for human: use, oil, and sesame (Sesamum indicum l.) The chemical composition of sesame was 16.69 g/100 g protein, 50.90 g/100 g fat, crude fiber 15.70 g/100 g, 5.01 g/100 g ash, 12.76 g/100 g carbohydrates The evaluation was conducted sesame toasting time, effect of temperature, the effect of added water, sugar substitute, effect of citric acid, in the crystalline state Results: Final formulation in 100 g solid: 23.67 g water; 35.51 g sucrose; 23.67 g glucose; 20 g roasted sesame, 0.47 g citric acid The best sesame seed was stripped of its bark, the addition of glucose to the formulation was essential and established for the production of hard candy; citric acid was added to sesame hard candy, which was added at the end and in the crystalline state In order to develop sesame energetic bar was Abstracts recommended the presence of amaranth In addition required the presence of a mold release agent (wax paper thin), a bonding agent (starch gels in cold) a baking process to achieve a desirable texture The effect of the size of final products is a feature to provide single servings Of manufactured products found greater acceptance sesame bar Key words: Hard candy; energetic bar; sesame; citric acid; glucose PO3385 EFFECT OF FEEDING OMANI HALWA ON THE GROWTH PERFORMANCE, BLOOD GLUCOSE AND PLASMA LIPID PROFILE OF SPRAGUE-DAWLEY RATS A Ali1, A Z Khalid1, M Waly1, D Sankar1,2, E Johnson1,3 Department of Food Scienec and Nutrition, Sultan Qaboos University, Oman Department of Cardiovascular Diseases, Fukuoka University Chikushi Hospital, Japan Department of Animal and Veterinary Sciences, Sultan Qaboos University, Oman Background and objectives: Omani halwa is a traditional sweet delicacy We evaluated effect of feeding different levels of Omani halwa on growth performance, blood glucose, glycated hemoglobin (HbA1c) and plasma lipid profile of Spraguedawley (SD) rats Methods: Forty-two male SD-rats of weeks old were randomly divided into groups containing rats in each Six experimental diets (in which normal rat chow was replaced with either white or black Omani halwa at 10, 15, and 20% level) were prepared and fed for 10 weeks The group fed on rat chow acted as control At the end rats were euthanized by cervical dislocation and blood (plasma) collected was analyzed for various biochemical parameters Results: No significant (P < 0.05) differences were observed in feed consumption and growth rate of rats fed different levels and types of halwa as compared to control The blood glucose level in rats fed different experimental diets did not vary (P < 0.05) as compared to control The HbA1c values however differed significantly (P < 0.05) and the rats fed diets containing 20% of halwa showed significantly (P < 0.05) higher HbA1c values as compared to control group Similarly rats fed diets containing 20% of halwa showed significantly (P < 0.05) higher TC and HDL-C values as compared to control No significant (P < 0.05) differences were however observed in TG, LDL-C, and TC/HDL-C ratio of rats fed various experimental diets The plasma creatinine levels differed significantly (P < 0.05) whereas plasma albumin levels did not differ (P > 0.05) in rats fed various experimental diets Abstracts Conclusion: Feeding Omani halwa at 15% level did not affect the growth, blood glucose, HbA1c levels and lipid profile of rats Acknowledgement: Financial assistance was provided by SQU (IG/AGR/FOOD/01/11) Key words: Omani halwa, feeding rats, blood glucose, HbA1c, lipid profile PO3386 MUSHROOM PROTEIN CONCENTRATES AS A PROTEIN SOURCE OF HIGH NUTRITIONAL VALUE M Dớaz Herrero1, P Carbonero Aguilar1, O Creamades2, L Soares de Assunỗọo3, J Bautista1 Dpto Bioquớmica Y Biologớa Molecular U Sevilla, Sevilla, Spain Escuela Universitaria de Osuna,Sevilla, Spain Universidad Federal de Viỗosa, Brasil Background and objectives: Nowadays is well accepted that a diet based in vegetable proteins is healthier than one based in animal proteins Usually industrial protein concentrates are obtained by a wet procedure, i.e, protein extraction in alkaline solution and precipitation at its pI This procedure implies a denaturalization of protein and loss of functional properties, and the reduction of other important functional products (polyphenols, vitamins, etc.) However, obtaining of protein concentrates by dry procedures (selective screening and elutration) allows obtaining products with 40-50% native proteins retaining main of the others functional compounds In this work we present the characterization of mushroom protein concentrates obtained from industrial mushroom (Agaricus bisporus) by-products, by a dry procedure Methods: Mushroom were made into flour and fractionated by a dry procedure attaining four fractions: F-I, F-II, F-III and F-IV, which were analysed (humidity, ashes, proteins, carbohydrates, lipids, aminogram, solubility, water and oil retention Results: Mushroom flour (18.9%) has been concentrated by a dry procedure Fractions F-III and F-IV present a protein concentration of 45.1% and 56.7%, respectively F-IV amino acidic composition is well equilibrated (46.36 mg AEE/100 mg protein) This product can be used for the preparation of food product or formulas The content in AEE covers the FAO and OMS recommendations for infants (45.6 mg AEE/ 100 mg protein), pre-schoolers (32.0 mg AEE/ 100 mg protein) and adults (11.1 mg /100mg protein) Conclusion: The main advantage of this product is that the proteins conserve their native conformations retaining, practically, all its functional properties, and it conserves other functional products (polyphenol, vitamins, fibre) constituting a good sources for the preparation of food products or formulas for young and old peoples Ann Nutr Metab 2013;63(suppl 1):11960 1713 Acknowledgements: This work was supported by funds from the Spanish Ministerio de Ciencia e Innovaciún and EU funds (FEDER) (Project: IPT-2011-1418-060000) Key words: Mushroom, protein, high nutritional value PO3387 OLIVE LEAF BIOACTIVES: EXTRACT COMPOSITION, ABSORPTION AND METABOLIC EFFECTS IN HUMANS S Hodgkinson1, M de Bock1, R Schlothauer2, W Cutfield1 Liggins Institute, University of Auckland, Australia Comvita New Zealand Ltd, Te Puke, New Zealand Bacground and objectives: Studies of the metabolic benefits of bioactives in olive leaf (from Olea europaea L.) are confounded by research using virgin olive oil owing to the differing phenolic composition of the two sources Objectives of the current study were: To profile the bioactives in Olive Leaf Extract (OLE); To examine the bioavailability of key bioactives in OLE and; To determine effects of OLE on insulin-sensitivity (IS) in a population at risk of developing type two diabetes Methods and results: Analysis of OLE phenolics revealed OL and hydroxytyrosol (HT) to be the major constituents (83.7% w/w and 12.4% w/w abundance respectively) 2.Volunteers received either encapsulated or liquid OLE as acute oral doses and plasma and urine samples collected Conjugated metabolites of HT (sulphated and glucuronidated) were the major forms identified in plasma and urine Peak OL concentrations were 15 20 fold lower than HT metabolites in plasma and greater following liquid than capsules (p=0.004) Similarly the time-to-peak conjugated HT concentration in plasma was shorter for liquid than capsules (93 vs 64 minutes; p=0.031) Conjugated HT species were the major forms identified in urine with >80% being cleared within 8hours The data shows that OLE effectively delivers oleuropein and hydroxytyrosol metabolites to plasma in humans Effect of OLE on IS was determined using encapsulated OLE (51.1 mg oleuropein, 9.7 mg hydroxytyrosol per day) in middle-aged, overweight males using a gold-standard trial design Participants (n = 46) received capsules containing OLE or placebo for 12 weeks before washing out and crossing over to the other treatment OLE supplementation was found to improve IS by 15% (p=0.024) and pancreatic -cell responsiveness by 28% (p=0.013) Conclusion: We conclude supplementation with OLE is a useful preventative treatment for those at risk of developing type diabetes Key words: Olive leaf, oleuropein, hydroxytyrosol, insulinsensitivity 1714 Ann Nutr Metab 2013;63(suppl 1):11960 PO3388 TREATMENT WITH A SYNBIOTIC CONCEPT REDUCES ASTHMA-LIKE SYMPTOMS AND INDUCES REGULATORY RESPONSES IN A MURINE MODEL OF CHRONIC ALLERGIC ASTHMA G Folkerts1, S Sagar1,2, M Morgan1, J Garssen1,2, N Georgiou1, A Kraneveld1, P Vos1,2 Utrecht Institute for Pharmaceutical Sciences, Faculty of Science, Utrecht University, Utrecht, The Netherlands Danone Research, Centre for Specialised Nutrition, Wageningen, The Netherlands Background and objectives: Asthma is a chronic inflammatory disease affecting over 300 million people worldwide Despite the effectiveness of the current treatments, over 50% of asthmatics are poorly controlled This study explores the therapeutic effects of long-term treatment with a specific combination of Bifidobacterium breve M-16V and non-digestible oligosaccharides on asthma-like symptoms in a chronic allergic asthma model Methods: Animals were sensitized (intraperitoneally) and challenged with ovalbumin (aerosol) After the first tree aerosol challenges, mice received saline or a synbiotic concept of Bifidobacterium breve M-16V, short- and long-chain fructo-oligosaccharides and pectin-derived acidic oligosaccharides by oral gavage and were challenged with ovalbumin tree times a week for one month Pulmonary airway inflammation, Th-specific cytokines and transcription factors in lung tissue, mast cell degranulation, airway remodeling and other parameters were examined Results: B breve M-16V and non-digestible oligosaccharides suppressed the influx of pulmonary eosinophils, but not macrophages, lymphocytes and neutrophils Reduced levels of serum mouse mast cell protease-1 indicates a reduction in mucosal mast cell The expression ratios of T cell transcription factors in lung tissue suggest that active treatment stimulates a regulatory T-cell phenotype, which is supported by the cytokine expression profile In addition, the Foxp3 mean fluorescence intensity in blood CD4+ T cells was increased Conclusion: B breve M-16V with non-digestible oligosaccharides, administered in a therapeutic way, has strong antiinflammatory properties and induces regulatory responses in a chronic allergic asthma model Interestingly, oral gavage with the concept leads to modulation of immune responses in the lungs, showing immunomodulatory effects that are not limited to the gastro-intestinal tract This concept may be beneficial in the treatment of chronic asthma and/or allergic conditions Key words: B breve M-16V, non-digestible oligosaccharides, allergy, therapy, immune response asthma and/or allergic conditions Abstracts PO3389 LEAF CONCENTRATES; LOW COST NATURAL SUPPLEMENTS TO COMBAT MICRO NUTRIENT DEFICIENCIES S Sapur1, J Avvari2 Akkshaya Foundation,Hyderabad, A.P, India Professor in Food and Nutrition, Dept of Home Science, Sri Padmavathi Mahila Viswa Vidyalayam, Tirupati, A.P, India Background and objectives: The World Health organization has estimated the prevalence of clinical and sub clinical vitamin A deficiency in India is among the highest in the world According to National Family Health Survey (NFHS), seventy three percent of Indian population suffers with anemia Green leafy vegetables (GLV) are treasure trove of micronutrients but seasonal Preservation of these GLV, ensures availability of nutrients round the year To preserve, develop nutrient dense leaf concentrates from seasonal GLV and to find out the appropriate dehydration method to minimize the nutrient losses Methods: seasonal locally available four GLV (piper betel,Murraya Koenigii,Trigonella foenum graecum, Mentha Spicata) were selected for the study, they were pretreated, blanched at 900C for minute with magnesium oxide, and dried in solar cabinet driers which were with and without Ultra violet (UV) reduction filter Time, temperature and yield of the leaf concentrate were noted The leaf concentrates were analysed for carotene and iron content Nutritionally dense traditional food products were developed using leaf concentrate, and organolepticaly evaluated Results: Carotene and iron concentration of leaf concentrates increased by to times in comparison with fresh green leafy vegetables The nutrient and yield of the leaf concentrate was much higher from solar cabinet drier with UV reduction filter The carotene content ranged from10821 - 14017àg/100g and iron content varied from 21.7mg- 88.1 mg/100gm Statistical analysis of the organoleptic evaluation of traditional food items with leaf concentrate was excellent, (P value