Nghiên cứu nhân nhanh in vitro loài lan Kim tuyến (Anoectochilus setaceus Blume) nhằm bảo tồn nguồn dược liệu quý

Header Page of 16 I HC QUC GIA H NI TRNG I HC KHOA HC T NHIấN Phớ Th Cm Min NGHIấN CU NHN NHANH IN VITRO LOI LAN KIM TUYN (ANOECTOCHILUS SETACEUS BLUME) NHM BO TN NGUN DC LIU QUí LUN VN THC S KHOA HC Footer Page of 16 Header Page of 16 I HC QUC GIA H NI TRNG I HC KHOA HC T NHIấN Phớ Th Cm Min NGHIấN CU NHN NHANH IN VITRO LOI LAN KIM TUYN (ANOECTOCHILUS SETACEUS BLUME) NHM BO TN NGUN DC LIU QUí Chuyờn ngnh: Sinh hc Thc nghim Mó s: 60 42 30 LUN VN THC S KHOA HC NGI HNG DN KHOA HC: PGS.TS Nguyn Trung Thnh Footer Page of 16 Header Page of 16 LI CM N Trong quỏ trỡnh thc tt nghip v hon thnh lun vn, em ó nhn c s giỳp v nhiu mt ca cỏc cp lónh o, cỏc th v cỏc cỏ nhõn Trc tiờn, em xin by t lũng bit n chõn thnh n PGS.TS Nguyn Trung Thnh, thy ó luụn tn tỡnh hng dn, giỳp em sut quỏ trỡnh thc hin v hon thnh lun ny c bit, em xin by t lũng bit n sõu sc ti thy GS.TS Nguyn Quang Thch v ton th th cỏn b phũng Cụng ngh t bo Thc vt, vin Sinh hc Nụng nghip, i hc Nụng nghip H Ni ó nhit tỡnh giỳp , to iu kin v c s vt cht, chia s kinh nghim quý bỏu em hon thnh tt ti ny Cui cựng em xin gi li cm n chõn thnh v sõu sc nht ti gia ỡnh, ngi thõn v bn bố ó c v, ng viờn, giỳp em sut quỏ trỡnh hc v thc hin ti Em xin chõn thnh cm n! H Ni, ngy thỏng 12 nm 2012 Hc viờn Phớ Th Cm Min Footer Page of 16 Header Page of 16 DANH MC CC CH VIT TT BAP 6-benzylaminopurin CT Cụng thc CV% H s bin ng (Correlation of Variance) C i chng IBA indol-3-acetic acid MS Murashige v Skoog, 1962 LSD0,05 Sai khỏc ti thiu cú ý ngha P - 0,5 (Leant Significant Difference) ND Nc da NAA -naphthylacetic acid Footer Page of 16 Header Page of 16 MC LC LI CM N MC LC M U 1.1 t CHNG I TNG QUAN TI LIU 1.1 Gii thiu chung v cõy Lan Kim tuyn 1.1.1 c im hỡnh thỏi 1.1.2 c im phõn b 11 1.2 Nhõn ging cõy Lan Kim tuyn (Anoectochilus setaceus) 13 1.2.1 Nhõn ging bng ht 13 1.2.2 Nhõn ging bng cõy 13 1.2.3 Phng phỏp giõm cõy 14 1.2.4 Nhõn ging in vitro 14 1.2.4.1 C s khoa hc ca nhõn ging in vitro 14 1.2.4.2 í ngha ca nhõn ging in vitro 15 1.2.4.3 Cỏc phng thc nhõn ging vụ tớnh 18 1.3 Cỏc yu t nh hng n nuụi cy 18 1.3.1 nh hng ca iu kin kh trựng mu cy 18 1.3 nh hng ca cỏc thnh phn húa hc 19 1.3 nh hng ca cỏc yu t vt lý 23 1.3.4 nh hng ca iu kin cõy 24 1.4 Quy trỡnh sn xut cõy cy mụ 25 1.5 Tỡnh hỡnh nghiờn cu trờn cõy Lan Kim tuyn 26 1.5.1.Tỡnh hỡnh nghiờn cu trờn th gii 26 1.5.2 Tỡnh hỡnh nghiờn cu nc 27 CHNG II: NI DUNG V PHNG PHP NGHIấN CU 28 2.1 i tng, vt liu, a im v thi gian nghiờn cu 28 2.2 Mc tiờu nghiờn cu 28 2.3 í ngha khoa hc v thc tin 29 Footer Page of 16 Header Page of 16 2.4 Ni dung nghiờn cu 29 2.5 Phng phỏp nghiờn cu 29 2.5.1 Phng phỏp b trớ thớ nghim 30 2.5.2 Phng phỏp ỏnh giỏ kt qu v x lý s liu 35 CHNG III KT QU V THO LUN 39 3.1 Nghiờn cu phng phỏp kh trựng mu sch v xỏc nh c quan vo mu phự hp cho vic nhõn nhanh in vitro 39 3.1.1 Xỏc nh cht kh trựng thớch hp 39 3.1.2 Xỏc nh c quan vo mu thớch hp to mu sch v tng h s nhõn chi in vitro 42 3.1.2.1 nh hng ca loi vt liu n t l to mu sch 42 3.1.2.2 nh hng ca c quan vo mu n h s nhõn chi in vitro 44 3.2 Nghiờn cu mụi trng ng v nhõn nhanh thớch hp 45 3.2.1 Xỏc nh mụi trng nn thớch hp cho nuụi cy mụ Lan Kim tuyn (A setaceus) 45 3.2.2 Nghiờn cu nh hng ca nhúm cht iu hũa sinh trng riờng r v phi hp n s phỏt sinh hỡnh thỏi v h s nhõn 47 3.2.2.1 nh hng ca nhúm cht Cytokinin n s phỏt sinh hỡnh thỏi v h s nhõn 47 3.2.2.2 Nghiờn cu nh hng ca IBA v NAA n s phỏt sinh hỡnh thỏi v h s nhõn Lan Kim tuyn (A setaceus) 50 3.2.2.3.nh hng ca s phi hp nhúm cht l auxin v cytokinin n s phỏt sinh hỡnh thỏi v h s nhõn 53 3.3 Nghiờn cu r to cõy hon chnh 56 3.3.1 nh hng ca mụi trng nn (mụi trng khụng cú cht iu tit sinh trng) n s r 56 3.3.2 Nghiờn cu nh hng ca than hot tớnh n s r 58 CHNG IV KT LUN V NGH 67 4.1 Kt lun 67 4.2 ngh 68 TI LIU THAM KHO 69 PH LC 75 Footer Page of 16 Header Page of 16 M U 1.1 t T xa n nay, lan c bit n nh mt loi hoa quý phỏi, hoa ca bc vua chỳa vng gi Lan c phỏt hin u tiờn vo nhng nm 40 ca th k VIII, cho n ó cú hn 15.000 loi lan trng khỏc trờn trờn th gii Ti Vit Nam, hoa lan cng vụ cựng a dng phong phỳ, cú khong hn 1.000 loi hoa cỏc loi, chỳng sinh sn ti cỏc vựng rng, nỳi nh Cao Bng, Lo Cai, Hu, Quy Nhn, Lt, Pleiku, lan Vit Nam p cao li cha ng nhiu ý ngha, cú rt nhiu cõy quý him v cú nhng cõy trc ch thy mc Vit Nam (nh lan n hi Paphiopedilum delenati) H lan (Orchidaceae) l mt s nhng h thc vt a dng nht ca Vit Nam, vi tng s khong 865 loi thuc 154 chi Thụng thng lan c s dng lm cnh Ngoi ra, cú nhiu loi lan cũn c s dng lm thuc Chi lan Kim tuyn Anoectochilus Vit Nam hin thng kờ c 12 loi, ú cú loi lan Kim tuyn Anoectochilus setaceus Blume, tờn khỏc Anoectochilus roxburghii Wall ex Lindl phõn b rng hu ht cỏc tnh c nc, c bit n nhiu khụng nhng bi giỏ tr lm cnh, m bi giỏ tr lm thuc ca nú Do b thu hỏi nhiu bỏn lm thuc t rt lõu, nờn loi lan Kim tuyn ang b e da nghiờm trng, rt cú th s b tuyt chng ngoi t nhiờn nu chỳng ta khụng cú bin phỏp bo tn hu hiu Hin nay, lan Kim tuyn c cp bỏo Ngh nh 32/2006/N-CP thuc nhúm IA, nghiờm cm khai thỏc s dng vỡ mc ớch thng mi v nhúm thc vt rng ang nguy cp EN A1a,c,d, sỏch Vit Nam 2007 Vỡ vy, nghiờn cu k thut nhõn ging loi lan Kim tuyn - Anoectochilus setaceus Blume c trin khai s cung cp nhng c s khoa hc v thc tin to hng lot nhng cõy n nh v mt di truyn nhm bo tn v phỏt trin loi dc liu nguy cp, quớ him ny Xut phỏt t nhng c s ú, chỳng tụi ó la chn v thc hin nghiờn cu ti:Nghiờn cu nhõn nhanh in vitro loi lan Kim tuyn (Anoectochilus setaceus Blume) nhm bo tn ngun dc liu quý Footer Page of 16 Header Page of 16 CHNG I TNG QUAN TI LIU 1.1 Gii thiu chung v cõy Lan Kim tuyn Chi lan Kim tuyn (Anoectochilus) cũn c gi l lan trang sc vỡ v p hp dn ca nú, c Carlvon Blume mụ t u tiờn nm 1810 thuc phõn h Orchidoideae Trờn th gii ó thng kờ c 51 loi Vit Nam hin thng kờ c 12 loi, ú loi lan Kim tuyn (Anoectochilus setaceus Blume) c bit n ch yu vi cụng dng lm thuc Lan Kim tuyn (Anoectochilus setaceus Blume) ng danh l (Anoectochilus roxburghi Wall.) H: Phong lan (Orchidaceae) B: Phong lan (Orchidales) õy l loi n thõn, mc t, cú thõn r mc di; thõn trờn t mng nc v cú nhiu lụng mm, mang - lỏ mc xoố sỏt t Lỏ hỡnh trng, gn trũn gc, chúp hi nhn v cú mi ngn, c - x - cm, mt trờn mu nõu thm cú vt vng gia v mu hng nht trờn cỏc gõn, mt di mu nõu nht Cung lỏ di - cm Cm hoa di 10 - 15 cm, mang - 10 hoa mc tha Lỏ bc hỡnh trng, di 8-10 mm, mu hng Hoa thng mu trng, di 2,5 - cm; mụi di n 1,5 cm, mi bờn gc mang - di hp, ch ụi thnh thu hỡnh thuụn trũn Bu di 13mm, cú lụng tha Mựa hoa thỏng 2, thỏng Tỏi sinh bng chi t thõn r v ht ớt, sinh trng rt chm L loi cõy a búng, k ỏnh sỏng trc tip thng mc di tỏn rng nguyờn sinh, rng rm nhit i cao 500 - 1600m Mc ri rỏc vi ba cõy trờn t m, giu mựn v lỏ cõy rng Phõn b: Vit Nam: Lo Cai (Sapa), H Giang (Qun B), Yờn Bỏi, Vnh Phỳc (Tam o), H Tõy (M c: Chựa Hng), Qung Tr (ng Chố), Kontum (c Tụ: c Uy), Gia Lai (Kbang: Kon H Nng) Th gii: Trung Quc (Võn Nam, Qung ụng), n , Lo, Inụnờxia, Tỏc dng dc lý: Lan Kim tuyn l loi cõy thuc rt c bit cú tỏc dng tng cng sc kho, lm khớ huyt lu thụng, cú tớnh khỏng khun, cha cỏc bnh viờm khớ qun, viờm gan tớnh, cha suy nhc thn kinh Loi lan ny c dựng lm thuc cha bnh tr lao phi, phong thp, au nhc khp xng, viờm d dy tớnh (Nguyn Tin Bõn, Dng c Huyn) Trc ú, lan Kim tuyn (A setaceus) l mt nhng dc tho quý giỏ, giỳp b mỏu, Footer Page of 16 Header Page of 16 dng õm, cha tr núng phi v núng gan (T A Mc v Trn Kin o, 1958) Hn na mi õy ngi ta ó phỏt hin kh nng phũng v chng ung th ca loi tho dc ny Theo cỏc ti liu nghiờn cu ca Trung Quc cụng b gn õy bng k thut sc ký lng, sc ký ct v k thut quang ph ó phõn lp, xỏc nh c cu trỳc hoỏ hc v th hot tớnh sinh hc ca mt s hp cht cú loi lan Kim tuyn (Tp Sinh hc thc vt tng hp Trung Quc, 48 s 3, thỏng 3/2006, trang 359-363) Bng cỏc k thut quang ph ó xỏc nh c hp cht hoỏ hc Cỏc hp cht ny u cú hot tớnh sinh hc mnh, cú kh nng lm gim cỏc gc t c th, nờn cú kh nng phũng bnh rt tt c bit cú hai axớt hu c c phõn lp l Olenolic acid v Ursolic acid cú kh nng chng ung th, gim cholesterol mỏu, chng tng huyt ỏp, khỏng khun 1.1.1 c im hỡnh thỏi Lan Kim tuyn l cõy tho, mc t, cú thõn r mc di; thõn trờn t mng nc, mang cỏc lỏ mc xũe sỏt t a Thõn r Lan Kim tuyn l cõy thõn r nm ngang sỏt mt t, ụi hi nghiờng, bũ di Chiu di thõn r t 5-12 cm, trung bỡnh l 7,87 cm ng kớnh thõn r t 3-4 mm, trung bỡnh l 3,17 mm S lúng trờn thõn r t 3-7 lúng, trung bỡnh l 4,03 lúng Chiu di ca lúng t 1-6 cm, trung bỡnh l 1,99 cm Thõn r thng cú mu xanh trng, ụi cú mu nõu , thng nhn, khụng ph lụng b Thõn khớ sinh Cõy lan Kim tuyn cú thõn khớ sinh thng mc thng ng trờn mt t, ớt mc nghiờng Chiu di thõn khớ sinh t 4-8 cm, trung bỡnh cm ng kớnh thõn khớ sinh t 3- mm, trung bỡnh l 3,08 cm Thõn khớ sinh mang nhiu lúng, cỏc lúng cú chiu di khỏc S lúng trờn thõn khớ sinh thay i t 2-4 lúng, trung bỡnh l 2,87 Chiu di mi lúng t 1-4 cm, trung bỡnh 2,23 cm Thõn khớ sinh thng mng nc, nhn, khụng ph lụng; thng cú mu xanh trng, ụi cú mu hng nht c R R lan Kim tuyn c mc t cỏc mu trờn thõn r ụi r cng c hỡnh thnh t thõn khớ sinh R thng õm thng xung t Thụng thng mi mu ch cú mt r, ụi cú vi r cựng c hỡnh thnh t mt Footer Page of 16 Header Page 10 of 16 mu trờn thõn r S lng v kớch thc r cng rt thay i tu theo cỏ th S r trờn mt cõy thng t - 10, trung bỡnh l 5,4 Chiu di ca r thay i t 0,5 - cm, r di nht trung bỡnh l 6,07cm v ngn nht trung bỡnh l 1,22 cm, chiu di trung bỡnh ca cỏc r trờn mt cõy l 3,82 cm d Lỏ Lỏ lan Kim tuyn mc cỏch xon quanh thõn, xoố trờn mt t Lỏ hỡnh trng, gn trũn gc, u lỏ hi nhn v cú mi ngn, thng di t - cm, trung bỡnh l 4,03 cm v rng t - cm, trung bỡnh l 3,12 cm Lỏ cú mu nõu mt trờn v ph lụng mn nh nhung H gõn lỏ mng li lụng chim, thng cú gõn gc Cỏc gõn ny thng cú mu hng mt trờn v ni rt rừ ụi gõn gia cú mu vng nht Mt di lỏ cú mu nõu nht, nhn vi gõn gc ni rừ Cỏc gõn bờn phớa rỡa lỏ ni rừ, gõn gia lỏ mt di khụng rừ Cung lỏ di 0,6 - 1,2 cm, thng nhn v cú mu trng xanh, ụi hi tớa b lỏ B lỏ ni rừ v nhn S lỏ trờn mt cõy thay i t - 6, thụng thng cú lỏ Kớch thc ca lỏ cng thay i, cỏc lỏ trờn mt cõy thng cú kớch thc khỏc rừ rt e Hoa, qu Hoa lan Kim tuyn dng cm, di 10 - 20 cm ngn thõn, mang - 10 hoa mc tha Lỏ bc hỡnh trng, di - 10 mm, mu hng Cỏc mnh bao hoa di khong mm; cỏnh mụi mu trng, di n 1,5 cm, mi bờn gc mang - di hp, u ch ụi Mựa hoa thỏng 10 - 12 Mựa qu chớn thỏng 12 - nm sau Footer Page 10 of 16 Header Page 75 of 16 PH LC PH LC 1: CC MễI TRNG S DNG TRONG CC TH NGHIM * Mụi trng KnudsonC (1965) Thnh phn a lng Lng ly 1l dung dch m (g) KCl 5,00 KH2PO4 5,00 MgSO4 2,44 NH4NO3 10,00 (NH4)2SO4 10,00 Ca(NO3)2 (Pha riờng) Vi lng 1l mụi trng s dng 50ml/l 4,82 (mg) 10ml/l MnSO4.H2O St 568,00 (g) ml/l FeSO4.7H2O Footer Page 75 of 16 5,00 Header Page 76 of 16 * Mụi trng c bn MS (Murashige v Skoog, 1962) Lng ly Thnh phn a lng 1l dung dch m (g) NH4NO3 33,00 KNO3 38,00 MgSO4.7H2O 10,00 KH2PO4 3,40 CaCl2 (Pha riờng) 6,60 Vi lng 620,000 MnSO4.4H2O 2230,00 ZnSO4.4H2O 860,00 KI 83,00 MoNa2.2H2O 25,00 CoCl2.6H2O 2,50 CuSO4.5H2O 2,50 St (g) FeSO4.7H2O 5,56 Na2EDTA 7,46 Vitamin (mg) Glycine 400 Axit Nicotinic (B5) 100 Pyridixine (B6) 100 Inositol Footer Page 76 of 16 50ml/l (mg) H3BO3 Thiamine HCl (B1) 1l mụi trng s dng 20 100 10ml/l ml/l 5ml/l Header Page 77 of 16 Môi trờng Knudson cải tiến (Knud*) STT Tên dung dịch Thành phần Ammonium nitrate NH4NO3 K1 K2 1900 Magnesium sulfate MgSO4.7H2O 146.5 Postasium phosfate KH2PO4 170 Postasium chlorid KCl 300 Calcium chlorid CaCl2.2H2O 453 Postasium Iodide KI 0.75 Manganese sulfate MnSO4 4H2O Footer Page 77 of 16 Zinc sulfate 7H2O Molipdic acid (Na2).H2O K4 K5 600 Postasium nitrate KNO3 Boric acid H3BO4 K3 Hàm lợng (mg/l) 3,0 10,0 2,0 0,25 Cupric sulfate.5H2O 0,025 Cobal chlorid 6H2O 0,025 Ferrous sulfate 27,8 Ethylene diamine tetra acetic acid 2Na.2H2O 37,3 Glycin 2,0 Thiamine.HCl 0,4 Myo inositol 100 Pyridoxine HCl 0,5 Nicotinic acid 0,5 Header Page 78 of 16 PH LC 2: BNG KT QU X Lí THNG Kấ Bng 3.3 nh hng ca c quan vo mu n h s nhõn chi in vitro BALANCED ANOVA FOR VARIATE TAOMAU FILE TN3S2 30/ 6/12 10:37 :PAGE Thiet ke hoan toan ngau nhien VARIATE V003 TAOMAU Ty le tao mau (%) LN SOURCE OF VARIATION SUMS OF MEAN F RATIO PROB ER SQUARES SQUARES LN ============================================================================= CQ$ 7222.78 3611.39 ****** 0.000 * RESIDUAL 149596E-03 249327E-04 * TOTAL (CORRECTED) 7222.78 902.847 BALANCED ANOVA FOR VARIATE HSN FILE TN3S2 30/ 6/12 10:37 :PAGE Thiet ke hoan toan ngau nhien VARIATE V004 HSN LN DF He so nhan (Lan) SOURCE OF VARIATION DF SUMS OF MEAN F RATIO PROB ER SQUARES SQUARES LN ============================================================================= CQ$ 9.38829 4.69414 306.58 0.000 * RESIDUAL 918669E-01 153112E-01 * TOTAL (CORRECTED) 9.48015 1.18502 BALANCED ANOVA FOR VARIATE CHIEUCAO FILE TN3S2 30/ 6/12 10:37 :PAGE Thiet ke hoan toan ngau nhien VARIATE V005 CHIEUCAO Chieu cao TB choi (cm) LN SOURCE OF VARIATION DF SUMS OF MEAN F RATIO PROB ER SQUARES SQUARES LN ============================================================================= CQ$ 581667 290833 45.52 0.000 * RESIDUAL 383334E-01 638890E-02 * TOTAL (CORRECTED) 620000 775000E-01 TABLE OF MEANS FOR FACTORIAL EFFECTS FILE TN3S2 30/ 6/12 10:37 :PAGE Thiet ke hoan toan ngau nhien MEANS FOR EFFECT CQ$ CQ$ CHOI DINH CHOI NACH MAT DOT NOS 3 TAOMAU 33.3300 50.0000 100.000 HSN 2.11333 3.50000 4.61000 CHIEUCAO 2.58333 2.96667 3.20000 SE(N= 3) 0.288286E-02 0.714403E-01 0.461479E-01 5%LSD 6DF 0.997229E-02 0.247123 0.159633 ANALYSIS OF VARIANCE SUMMARY TABLE FILE TN3S2 30/ 6/12 10:37 :PAGE Thiet ke hoan toan ngau nhien Footer Page 78 of 16 Header Page 79 of 16 F-PROBABLIITY VALUES FOR EACH EFFECT IN THE MODEL SECTION - VARIATE TAOMAU HSN CHIEUCAO GRAND MEAN (N= 9) NO OBS 61.110 3.4078 2.9167 STANDARD DEVIATION C OF V |CQ$ SD/MEAN | BASED ON BASED ON % | TOTAL SS RESID SS | 30.047 0.49933E-02 0.0 0.0000 1.0886 0.12374 3.6 0.0000 0.27839 0.79931E-01 2.7 0.0004 | | | | Bng 3.4: nh hng ca mụi trng nn n kh nng nhõn nhanh chi, mt t Lan Kim tuyn BALANCED ANOVA FOR VARIATE SOCHOI FILE TN4D 30/ 6/12 1: :PAGE Thiet ke hoan toan ngau nhien VARIATE V003 SOCHOI So luong choi (choi/mau) LN SOURCE OF VARIATION DF SUMS OF MEAN F RATIO PROB ER SQUARES SQUARES LN ============================================================================= MT$ 5.02927 2.51463 391.55 0.000 * RESIDUAL 385334E-01 642223E-02 * TOTAL (CORRECTED) 5.06780 633475 BALANCED ANOVA FOR VARIATE SOLA FILE TN4D 30/ 6/12 1: :PAGE Thiet ke hoan toan ngau nhien VARIATE V004 SOLA So la/choi LN SOURCE OF VARIATION DF SUMS OF MEAN F RATIO PROB ER SQUARES SQUARES LN ============================================================================= MT$ 9.47469 4.73734 ****** 0.000 * RESIDUAL 196012E-01 326687E-02 * TOTAL (CORRECTED) 9.49429 1.18679 BALANCED ANOVA FOR VARIATE CHIEUCAO FILE TN4D 30/ 6/12 1: :PAGE Thiet ke hoan toan ngau nhien VARIATE V005 CHIEUCAO Chieu cao TB choi (cm) LN SOURCE OF VARIATION DF SUMS OF MEAN F RATIO PROB ER SQUARES SQUARES LN ============================================================================= MT$ 7.59055 3.79528 ****** 0.000 * RESIDUAL 200006E-01 333344E-02 * TOTAL (CORRECTED) 7.61056 951319 TABLE OF MEANS FOR FACTORIAL EFFECTS FILE TN4D 30/ 6/12 1: :PAGE Thiet ke hoan toan ngau nhien MEANS FOR EFFECT MT$ MT$ Knud* Footer Page 79 of 16 NOS SOCHOI 3.67000 SOLA 4.01667 CHIEUCAO 3.56667 Header Page 80 of 16 MS Kundson C 3 2.11333 2.05667 1.55333 2.35333 1.33333 2.21667 SE(N= 3) 0.462682E-01 0.329993E-01 0.333339E-01 5%LSD 6DF 0.160049 0.114150 0.115307 ANALYSIS OF VARIANCE SUMMARY TABLE FILE TN4D 30/ 6/12 1: :PAGE Thiet ke hoan toan ngau nhien F-PROBABLIITY VALUES FOR EACH EFFECT IN THE MODEL SECTION - VARIATE SOCHOI SOLA CHIEUCAO GRAND MEAN (N= 9) NO OBS 2.6133 2.6411 2.3722 STANDARD DEVIATION C OF V |MT$ SD/MEAN | BASED ON BASED ON % | TOTAL SS RESID SS | 0.79591 0.80139E-01 3.1 0.0000 1.0894 0.57157E-01 2.2 0.0000 0.97536 0.57736E-01 2.4 0.0000 | | | | Bng 3.5: nh hng ca nhúm cht Cytokinin n s phỏt sinh hỡnh thỏi v h s nhõn BALANCED ANOVA FOR VARIATE SOCHOI FILE TN5A3 30/ 6/12 14:41 :PAGE Thiet ke hoan toan ngau nhien VARIATE V003 SOCHOI So choi (choi/mau) LN SOURCE OF VARIATION DF SUMS OF MEAN F RATIO PROB ER SQUARES SQUARES LN ============================================================================= ND$ 29.8502 7.46256 831.64 0.000 * RESIDUAL 10 897333E-01 897333E-02 * TOTAL (CORRECTED) 14 29.9400 2.13857 BALANCED ANOVA FOR VARIATE SODOT FILE TN5A3 30/ 6/12 14:41 :PAGE Thiet ke hoan toan ngau nhien VARIATE V004 SODOT So dot (dot/choi) LN SOURCE OF VARIATION DF SUMS OF MEAN F RATIO PROB ER SQUARES SQUARES LN ============================================================================= ND$ 2.94997 737493 241.01 0.000 * RESIDUAL 10 306003E-01 306003E-02 * TOTAL (CORRECTED) 14 2.98057 212898 BALANCED ANOVA FOR VARIATE CHIEUCAO FILE TN5A3 30/ 6/12 14:41 :PAGE Thiet ke hoan toan ngau nhien VARIATE V005 CHIEUCAO Chieu cao TB choi (cm) LN SOURCE OF VARIATION SUMS OF MEAN F RATIO PROB ER SQUARES SQUARES LN ============================================================================= ND$ 1.60644 401610 183.11 0.000 * RESIDUAL 10 219333E-01 219333E-02 - Footer Page 80 of 16 DF Header Page 81 of 16 * TOTAL (CORRECTED) 14 1.62837 116312 TABLE OF MEANS FOR FACTORIAL EFFECTS FILE TN5A3 30/ 6/12 14:41 :PAGE Thiet ke hoan toan ngau nhien MEANS FOR EFFECT ND$ ND$ 0,1 0,5 1,0 2,0 NOS 3 3 SOCHOI 1.22333 2.44333 3.94333 5.22333 4.22333 SODOT 1.54333 2.15667 2.31000 2.92667 2.37000 CHIEUCAO 2.31667 3.03333 3.15667 3.23333 3.01667 SE(N= 3) 0.546911E-01 0.319376E-01 0.270390E-01 5%LSD 10DF 0.172333 0.100637 0.852010E-01 ANALYSIS OF VARIANCE SUMMARY TABLE FILE TN5A3 30/ 6/12 14:41 :PAGE Thiet ke hoan toan ngau nhien F-PROBABLIITY VALUES FOR EACH EFFECT IN THE MODEL SECTION - VARIATE SOCHOI SODOT CHIEUCAO GRAND MEAN (N= 15) NO OBS 15 3.4113 15 2.2613 15 2.9513 STANDARD DEVIATION C OF V |ND$ SD/MEAN | BASED ON BASED ON % | TOTAL SS RESID SS | 1.4624 0.94728E-01 2.8 0.0000 0.46141 0.55318E-01 2.4 0.0000 0.34105 0.46833E-01 1.6 0.0000 | | | | BALANCED ANOVA FOR VARIATE SOCHOI FILE TN5B3S 30/ 6/12 15:11 :PAGE Thiet ke hoan toan ngau nhien VARIATE V003 SOCHOI So choi (choi/mau) LN SOURCE OF VARIATION DF SUMS OF MEAN F RATIO PROB ER SQUARES SQUARES LN ============================================================================= ND$ 25.6624 6.41561 910.38 0.000 * RESIDUAL 10 704714E-01 704714E-02 * TOTAL (CORRECTED) 14 25.7329 1.83806 BALANCED ANOVA FOR VARIATE SODOT FILE TN5B3S 30/ 6/12 15:11 :PAGE Thiet ke hoan toan ngau nhien VARIATE V004 SODOT So dot (dot/choi) LN SOURCE OF VARIATION SUMS OF MEAN F RATIO PROB ER SQUARES SQUARES LN ============================================================================= ND$ 2.34983 587457 183.20 0.000 * RESIDUAL 10 320666E-01 320666E-02 * TOTAL (CORRECTED) 14 2.38189 170135 - Footer Page 81 of 16 DF Header Page 82 of 16 BALANCED ANOVA FOR VARIATE CHIEUCAO FILE TN5B3S 30/ 6/12 15:11 :PAGE Thiet ke hoan toan ngau nhien VARIATE V005 CHIEUCAO Chieu cao TB choi (cm) LN SOURCE OF VARIATION DF SUMS OF MEAN F RATIO PROB ER SQUARES SQUARES LN ============================================================================= ND$ 2.04933 512333 256.17 0.000 * RESIDUAL 10 200000E-01 200000E-02 * TOTAL (CORRECTED) 14 2.06933 147810 TABLE OF MEANS FOR FACTORIAL EFFECTS FILE TN5B3S 30/ 6/12 15:11 :PAGE Thiet ke hoan toan ngau nhien MEANS FOR EFFECT ND$ ND$ 0,1 0,5 1,0 2,0 NOS 3 3 SOCHOI 1.22333 2.33000 4.22333 4.88667 3.22333 SODOT 1.54333 1.97667 2.44667 2.68000 2.01667 CHIEUCAO 2.31667 2.45000 3.05000 3.23333 3.08333 SE(N= 3) 0.484670E-01 0.326938E-01 0.258199E-01 5%LSD 10DF 0.152721 0.103019 0.813594E-01 ANALYSIS OF VARIANCE SUMMARY TABLE FILE TN5B3S 30/ 6/12 15:11 :PAGE Thiet ke hoan toan ngau nhien F-PROBABLIITY VALUES FOR EACH EFFECT IN THE MODEL SECTION - VARIATE SOCHOI SODOT CHIEUCAO GRAND MEAN (N= 15) NO OBS 15 3.1773 15 2.1327 15 2.8267 STANDARD DEVIATION C OF V |ND$ SD/MEAN | BASED ON BASED ON % | TOTAL SS RESID SS | 1.3558 0.83947E-01 2.6 0.0000 0.41247 0.56627E-01 2.7 0.0000 0.38446 0.44721E-01 1.6 0.0000 | | | | Bng 3.6: nh hng ca s phi hp nhúm cht l auxin v cytokinin n s phỏt sinh hỡnh thỏi v h s nhõn BALANCED ANOVA FOR VARIATE SOCHOI FILE TN6S2 30/ 6/12 9:47 :PAGE Thiet ke hoan toan ngau nhien VARIATE V003 SOCHOI So luong choi (choi/mau) LN SOURCE OF VARIATION SUMS OF MEAN F RATIO PROB ER SQUARES SQUARES LN ============================================================================= CT$ 8.62642 4.31321 201.86 0.000 * RESIDUAL 128201 213668E-01 * TOTAL (CORRECTED) 8.75462 1.09433 - Footer Page 82 of 16 DF Header Page 83 of 16 BALANCED ANOVA FOR VARIATE SODOT FILE TN6S2 30/ 6/12 9:47 :PAGE Thiet ke hoan toan ngau nhien VARIATE V004 SODOT So dot (dot/choi) LN SOURCE OF VARIATION DF SUMS OF MEAN F RATIO PROB ER SQUARES SQUARES LN ============================================================================= CT$ 171267 856333E-01 40.99 0.001 * RESIDUAL 125333E-01 208889E-02 * TOTAL (CORRECTED) 183800 229750E-01 BALANCED ANOVA FOR VARIATE CHIEUCAO FILE TN6S2 30/ 6/12 9:47 :PAGE Thiet ke hoan toan ngau nhien VARIATE V005 CHIEUCAO Chieu cao TB choi (cm) LN SOURCE OF VARIATION DF SUMS OF MEAN F RATIO PROB ER SQUARES SQUARES LN ============================================================================= CT$ 260000 130000 14.18 0.006 * RESIDUAL 550000E-01 916666E-02 * TOTAL (CORRECTED) 315000 393750E-01 TABLE OF MEANS FOR FACTORIAL EFFECTS FILE TN6S2 30/ 6/12 9:47 :PAGE Thiet ke hoan toan ngau nhien MEANS FOR EFFECT CT$ CT$ CT1 CT2 CT3 NOS 3 SOCHOI 4.66667 4.33333 6.55667 SODOT 2.45000 2.52667 2.77333 CHIEUCAO 3.11667 3.21667 3.51667 SE(N= 3) 0.843936E-01 0.263874E-01 0.552771E-01 5%LSD 6DF 0.291931 0.912783E-01 0.191212 ANALYSIS OF VARIANCE SUMMARY TABLE FILE TN6S2 30/ 6/12 9:47 :PAGE Thiet ke hoan toan ngau nhien F-PROBABLIITY VALUES FOR EACH EFFECT IN THE MODEL SECTION - VARIATE SOCHOI SODOT CHIEUCAO GRAND MEAN (N= 9) NO OBS 5.1856 2.5833 3.2833 STANDARD DEVIATION C OF V |CT$ SD/MEAN | BASED ON BASED ON % | TOTAL SS RESID SS | 1.0461 0.14617 2.8 0.0000 0.15158 0.45704E-01 1.8 0.0005 0.19843 0.95743E-01 2.9 0.0059 | | | | Bng 3.7 nh hng ca IBA v -NAA n s Phỏt sinh hỡnh thỏi v h s nhõn BALANCED ANOVA FOR VARIATE SOCHOI FILE TN7S2 30/ 6/12 16:11 :PAGE Thiet ke hoan toan ngau nhien Footer Page 83 of 16 Header Page 84 of 16 VARIATE V003 SOCHOI So choi (choi.mau) LN SOURCE OF VARIATION DF SUMS OF MEAN F RATIO PROB ER SQUARES SQUARES LN ============================================================================= ND$ 175894 351789E-01 12.37 0.000 * RESIDUAL 12 341334E-01 284445E-02 * TOTAL (CORRECTED) 17 210028 123546E-01 BALANCED ANOVA FOR VARIATE SODOT FILE TN7S2 30/ 6/12 16:11 :PAGE Thiet ke hoan toan ngau nhien VARIATE V004 SODOT So dot (dot/choi) LN SOURCE OF VARIATION DF SUMS OF MEAN F RATIO PROB ER SQUARES SQUARES LN ============================================================================= ND$ 580761 116152 14.36 0.000 * RESIDUAL 12 970667E-01 808889E-02 * TOTAL (CORRECTED) 17 677828 398722E-01 BALANCED ANOVA FOR VARIATE CHIEUCAO FILE TN7S2 30/ 6/12 16:11 :PAGE Thiet ke hoan toan ngau nhien VARIATE V005 CHIEUCAO Chieu dai TB choi (cm) LN SOURCE OF VARIATION DF SUMS OF MEAN F RATIO PROB ER SQUARES SQUARES LN ============================================================================= ND$ 6.89111 1.37822 261.14 0.000 * RESIDUAL 12 633328E-01 527773E-02 * TOTAL (CORRECTED) 17 6.95444 409085 BALANCED ANOVA FOR VARIATE SORE FILE TN7S2 30/ 6/12 16:11 :PAGE Thiet ke hoan toan ngau nhien VARIATE V006 SORE So luong re (re/choi) LN SOURCE OF VARIATION DF SUMS OF MEAN F RATIO PROB ER SQUARES SQUARES LN ============================================================================= ND$ 943895 188779 38.35 0.000 * RESIDUAL 12 590667E-01 492223E-02 * TOTAL (CORRECTED) 17 1.00296 589977E-01 BALANCED ANOVA FOR VARIATE CHIEUDAI FILE TN7S2 30/ 6/12 16:11 :PAGE Thiet ke hoan toan ngau nhien VARIATE V007 CHIEUDAI Chieu dai TB re (cm) LN SOURCE OF VARIATION SUMS OF MEAN F RATIO PROB ER SQUARES SQUARES LN ============================================================================= ND$ 187361 374722E-01 33.72 0.000 * RESIDUAL 12 133333E-01 111111E-02 * TOTAL (CORRECTED) 17 200694 118056E-01 TABLE OF MEANS FOR FACTORIAL EFFECTS FILE TN7S2 30/ 6/12 16:11 :PAGE Thiet ke hoan toan ngau nhien MEANS FOR EFFECT ND$ Footer Page 84 of 16 DF Header Page 85 of 16 ND$ 0,5 1,0 1,5 2,0 3,0 SE(N= 5%LSD NOS 3 3 3 SOCHOI 1.33000 1.27667 1.17000 1.50000 1.27667 1.33000 3) 12DF ND$ 0,5 1,0 1,5 2,0 3,0 SODOT 2.12667 2.31000 2.52333 2.66000 2.39667 2.58667 CHIEUCAO 3.33333 4.11667 3.71667 3.55000 3.23333 2.11667 SORE 2.04333 2.43667 2.09333 1.96333 1.83000 1.71000 0.307920E-01 0.519259E-01 0.419433E-01 0.405061E-01 0.948807E-01 0.160001 0.129242 0.124813 NOS 3 3 3 CHIEUDAI 0.916667 1.11667 0.883333 0.983333 1.05000 1.16667 SE(N= 3) 0.192450E-01 5%LSD 12DF 0.593004E-01 ANALYSIS OF VARIANCE SUMMARY TABLE FILE TN7S2 30/ 6/12 16:11 :PAGE Thiet ke hoan toan ngau nhien F-PROBABLIITY VALUES FOR EACH EFFECT IN THE MODEL SECTION - VARIATE SOCHOI SODOT CHIEUCAO SORE CHIEUDAI GRAND MEAN (N= 18) NO OBS 18 1.3139 18 2.4339 18 3.3444 18 2.0128 18 1.0194 STANDARD DEVIATION C OF V |ND$ SD/MEAN | BASED ON BASED ON % | TOTAL SS RESID SS | 0.11115 0.53333E-01 4.1 0.0003 0.19968 0.89938E-01 3.7 0.0001 0.63960 0.72648E-01 2.2 0.0000 0.24289 0.70159E-01 3.5 0.0000 0.10865 0.33333E-01 3.3 0.0000 | | | | BALANCED ANOVA FOR VARIATE SOCHOI FILE TN7BS2 30/ 6/12 16:44 :PAGE Thiet ke hoan toan ngau nhien VARIATE V003 SOCHOI So choi (choi.mau) LN SOURCE OF VARIATION DF SUMS OF MEAN F RATIO PROB ER SQUARES SQUARES LN ============================================================================= ND$ 15.7208 3.14417 503.95 0.000 * RESIDUAL 12 748683E-01 623903E-02 * TOTAL (CORRECTED) 17 15.7957 929159 BALANCED ANOVA FOR VARIATE SODOT FILE TN7BS2 30/ 6/12 16:44 :PAGE Thiet ke hoan toan ngau nhien VARIATE V004 SODOT So dot (dot/choi) LN SOURCE OF VARIATION SUMS OF MEAN F RATIO PROB ER SQUARES SQUARES LN ============================================================================= ND$ 2.33313 466627 56.75 0.000 * RESIDUAL 12 986668E-01 822224E-02 Footer Page 85 of 16 DF Header Page 86 of 16 * TOTAL (CORRECTED) 17 2.43180 143047 BALANCED ANOVA FOR VARIATE CHIEUCAO FILE TN7BS2 30/ 6/12 16:44 :PAGE Thiet ke hoan toan ngau nhien VARIATE V005 CHIEUCAO Chieu dai TB choi (cm) LN SOURCE OF VARIATION DF SUMS OF MEAN F RATIO PROB ER SQUARES SQUARES LN ============================================================================= ND$ 325361 650722E-01 57.70 0.000 * RESIDUAL 12 135333E-01 112778E-02 * TOTAL (CORRECTED) 17 338895 199350E-01 BALANCED ANOVA FOR VARIATE SORE FILE TN7BS2 30/ 6/12 16:44 :PAGE Thiet ke hoan toan ngau nhien VARIATE V006 SORE So luong re (re/choi) LN SOURCE OF VARIATION DF SUMS OF MEAN F RATIO PROB ER SQUARES SQUARES LN ============================================================================= ND$ 7.63998 1.52800 301.91 0.000 * RESIDUAL 12 607340E-01 506117E-02 * TOTAL (CORRECTED) 17 7.70071 452983 BALANCED ANOVA FOR VARIATE CHIEUDAI FILE TN7BS2 30/ 6/12 16:44 :PAGE Thiet ke hoan toan ngau nhien VARIATE V007 CHIEUDAI Chieu dai TB re (cm) LN SOURCE OF VARIATION DF SUMS OF MEAN F RATIO PROB ER SQUARES SQUARES LN ============================================================================= ND$ 1.15569 231139 277.37 0.000 * RESIDUAL 12 999998E-02 833331E-03 * TOTAL (CORRECTED) 17 1.16569 685703E-01 TABLE OF MEANS FOR FACTORIAL EFFECTS FILE TN7BS2 30/ 6/12 16:44 :PAGE Thiet ke hoan toan ngau nhien MEANS FOR EFFECT ND$ ND$ 0,5 1,0 1,5 2,0 3,0 SE(N= 5%LSD 3) 12DF ND$ 0,5 1,0 1,5 2,0 3,0 Footer Page 86 of 16 NOS 3 3 3 SOCHOI 1.33000 2.05667 2.55667 2.77667 4.11333 3.67000 SODOT 2.12667 2.54333 2.87000 2.30000 1.97333 1.78667 CHIEUCAO 3.33333 3.23333 3.41000 3.33333 3.36667 3.66667 SORE 2.04333 3.67667 4.19667 3.42000 3.32333 3.21333 0.456035E-01 0.523521E-01 0.193888E-01 0.410738E-01 0.140520 0.161315 0.597435E-01 0.126562 NOS 3 3 3 CHIEUDAI 0.916667 1.56667 1.33333 0.833333 1.31667 1.11667 Header Page 87 of 16 SE(N= 3) 0.166666E-01 5%LSD 12DF 0.513556E-01 ANALYSIS OF VARIANCE SUMMARY TABLE FILE TN7BS2 30/ 6/12 16:44 :PAGE Thiet ke hoan toan ngau nhien F-PROBABLIITY VALUES FOR EACH EFFECT IN THE MODEL SECTION - VARIATE SOCHOI SODOT CHIEUCAO SORE CHIEUDAI GRAND MEAN (N= 18) NO OBS 18 2.7506 18 2.2667 18 3.3906 18 3.3122 18 1.1806 STANDARD DEVIATION C OF V |ND$ SD/MEAN | BASED ON BASED ON % | TOTAL SS RESID SS | 0.96393 0.78988E-01 2.9 0.0000 0.37822 0.90677E-01 4.0 0.0000 0.14119 0.33582E-01 1.0 0.0000 0.67304 0.71142E-01 2.1 0.0000 0.26186 0.28867E-01 2.4 0.0000 | | | | Bng 3.8 nh hng ca mụi trng nn (mụi trng khụng cú cht iu tit sinh trng) n s r BALANCED ANOVA FOR VARIATE TAOMAU FILE TN8SS 1/ 7/12 13:58 :PAGE Thietke hoan toan ngau nhien VARIATE V003 TAOMAU Ty le tao mau (%) LN SOURCE OF VARIATION DF SUMS OF MEAN F RATIO PROB ER SQUARES SQUARES LN ============================================================================= MT$ 2291.08 763.695 ****** 0.000 * RESIDUAL 128010E-03 160013E-04 * TOTAL (CORRECTED) 11 2291.08 208.280 BALANCED ANOVA FOR VARIATE SORE FILE TN8SS 1/ 7/12 13:58 :PAGE Thietke hoan toan ngau nhien VARIATE V004 SORE So luong re (re/choi) LN SOURCE OF VARIATION DF SUMS OF MEAN F RATIO PROB ER SQUARES SQUARES LN ============================================================================= MT$ 1.47340 491133 147.34 0.000 * RESIDUAL 266668E-01 333336E-02 * TOTAL (CORRECTED) 11 1.50007 136370 BALANCED ANOVA FOR VARIATE CHIEUDAI FILE TN8SS 1/ 7/12 13:58 :PAGE Thietke hoan toan ngau nhien VARIATE V005 CHIEUDAI Chieu dai TB choi (cm) LN SOURCE OF VARIATION SUMS OF MEAN F RATIO PROB ER SQUARES SQUARES LN ============================================================================= MT$ 1.45667 485556 291.33 0.000 * RESIDUAL 133335E-01 166668E-02 * TOTAL (CORRECTED) 11 1.47000 133636 TABLE OF MEANS FOR FACTORIAL EFFECTS FILE TN8SS 1/ 7/12 13:58 :PAGE Footer Page 87 of 16 DF Header Page 88 of 16 Thietke hoan toan ngau nhien MEANS FOR EFFECT MT$ MT$ MS MS/2 Knud* Knud*/2 NOS 3 3 TAOMAU 66.6700 83.3300 66.6700 100.000 SORE 1.25000 1.86667 1.50000 2.17000 CHIEUDAI 1.60000 2.30000 1.83333 2.46667 SE(N= 3) 0.230950E-02 0.333334E-01 0.235704E-01 5%LSD 8DF 0.753103E-02 0.108697 0.768605E-01 ANALYSIS OF VARIANCE SUMMARY TABLE FILE TN8SS 1/ 7/12 13:58 :PAGE Thietke hoan toan ngau nhien F-PROBABLIITY VALUES FOR EACH EFFECT IN THE MODEL SECTION - VARIATE TAOMAU SORE CHIEUDAI GRAND MEAN (N= 12) NO OBS 12 79.167 12 1.6967 12 2.0500 STANDARD DEVIATION C OF V |MT$ SD/MEAN | BASED ON BASED ON % | TOTAL SS RESID SS | 14.432 0.40002E-02 0.0 0.0000 0.36928 0.57735E-01 3.4 0.0000 0.36556 0.40825E-01 2.0 0.0000 | | | | Bng 3.9 nh hng ca than hot tớnh n s cm ng r BALANCED ANOVA FOR VARIATE TAOMAU FILE TN9S3 30/ 6/12 21:17 :PAGE Thiet ke hoan toan ngau nhien VARIATE V003 TAOMAU Ty le tao mau (%) LN SOURCE OF VARIATION DF SUMS OF MEAN F RATIO PROB ER SQUARES SQUARES LN ============================================================================= ND$ 2499.50 833.167 ****** 0.000 * RESIDUAL 544845E-03 681056E-04 * TOTAL (CORRECTED) 11 2499.50 227.227 BALANCED ANOVA FOR VARIATE SORE FILE TN9S3 30/ 6/12 21:17 :PAGE Thiet ke hoan toan ngau nhien VARIATE V004 SORE So luong re (re/choi) LN SOURCE OF VARIATION DF SUMS OF MEAN F RATIO PROB ER SQUARES SQUARES LN ============================================================================= ND$ 18.3798 6.12661 ****** 0.000 * RESIDUAL 170668E-01 213335E-02 * TOTAL (CORRECTED) 11 18.3969 1.67244 BALANCED ANOVA FOR VARIATE CHIEUDAI FILE TN9S3 30/ 6/12 21:17 :PAGE Thiet ke hoan toan ngau nhien VARIATE V005 CHIEUDAI Chieu dai TB choi (cm) LN SOURCE OF VARIATION Footer Page 88 of 16 DF SUMS OF SQUARES MEAN SQUARES F RATIO PROB ER LN Header Page 89 of 16 ============================================================================= ND$ 6.33167 2.11056 ****** 0.000 * RESIDUAL 133334E-01 166667E-02 * TOTAL (CORRECTED) 11 6.34500 576818 TABLE OF MEANS FOR FACTORIAL EFFECTS FILE TN9S3 30/ 6/12 21:17 :PAGE Thiet ke hoan toan ngau nhien MEANS FOR EFFECT ND$ ND$ NOS 3 3 TAOMAU 66.6700 100.000 100.000 100.000 SORE 1.25000 2.00000 3.83000 4.22333 CHIEUDAI 1.60000 2.88333 3.21667 3.50000 SE(N= 3) 0.476465E-02 0.266668E-01 0.235703E-01 5%LSD 8DF 0.155370E-01 0.869577E-01 0.768603E-01 ANALYSIS OF VARIANCE SUMMARY TABLE FILE TN9S3 30/ 6/12 21:17 :PAGE Thiet ke hoan toan ngau nhien F-PROBABLIITY VALUES FOR EACH EFFECT IN THE MODEL SECTION - VARIATE TAOMAU SORE CHIEUDAI Footer Page 89 of 16 GRAND MEAN (N= 12) NO OBS 12 91.667 12 2.8258 12 2.8000 STANDARD DEVIATION C OF V |ND$ SD/MEAN | BASED ON BASED ON % | TOTAL SS RESID SS | 15.074 0.82526E-02 0.0 0.0000 1.2932 0.46188E-01 1.6 0.0000 0.75949 0.40825E-01 1.5 0.0000 | | | | ... loài dược liệu nguy cấp, quí Xuất phát từ sở đó, lựa chọn thực nghiên cứu đề tài: Nghiên cứu nhân nhanh in vitro loài lan Kim tuyến (Anoectochilus setaceus Blume) nhằm bảo tồn nguồn dược liệu quý ... Phí Thị Cẩm Miện NGHIÊN CỨU NHÂN NHANH IN VITRO LOÀI LAN KIM TUYẾN (ANOECTOCHILUS SETACEUS BLUME) NHẰM BẢO TỒN NGUỒN DƯỢC LIỆU QUÝ Chuyên ngành: Sinh học Thực nghiệm Mã số: 60 42... công trình nghiên cứu kỹ thuật nhân giống, gây trồng cho loài quý công bố Do vậy, nói việc nghiên cứu nhân nhanh loài lan Kim tuyến Anoectochilus setaceus Blume phương pháp nhân giống in vitro vừa