Nghiên cứu phân loại phân tông xuân tiết (subtrib justiciinae nees) thuộc họ ô rô (fam acanthaceae juss ) ở việt nam

B GIÁO D C VÀ ÀO T O VI N HÀN LÂM KHOA H C VÀ CÔNG NGH VI T NAM H C VI N KHOA H C VÀ CÔNG NGH - V N HÀI NGHIểN C U PHÂN LO I PHÂN TÔNG XUÂN TI T (Subtrib JUSTICIINAE Nees) THU C H Ô RÔ (Fam ACANTHACEAE Juss.) VI T NAM LU N ÁN TI N S SINH H C HĨ N I - 2016 B GIÁO D C VÀ ÀO T O VI N HÀN LÂM KHOA H C VÀ CÔNG NGH VI T NAM H C VI N KHOA H C VÀ CÔNG NGH - V N HÀI NGHIểN C U PHÂN LO I PHÂN TÔNG XUÂN TI T (Subtrib JUSTICIINAE Nees) THU C H Ô RÔ (Fam ACANTHACEAE Juss.) VI T NAM LU N ÁN TI N S SINH H C Chuyên ngành: Th c v t h c Mã s Ng ih : 62.42.01.11 ng d n khoa h c: PGS TS Nguy n Kh c Khôi HĨ N I - 2016 i L IC M hoàn thành lu n án này, nh n đ h N c s giúp đ , ch b o t n tình c a ng i ng d n khoa h c PGS TS Nguy n Kh c Khôi Nhân d p này, xin bày t lòng bi t n sâu s c v nh ng s giúp đ Tôi xin chân thành c m n TS Joongku Lee, TS Deng Yun Fei, TS Ritesh Kumar Choudhary, c ng tác giúp đ cung c p tài li u, kinh nghi m đ hoàn thành b n lu n án Tôi xin chân thành c m n s giúp đ v kinh phí thu th p m u c a d án H p tác gi a Vi n Sinh thái Tài nguyên sinh v t Vi n Nghiên c u Sinh h c Công ngh sinh h c Hàn Qu c (Ch nhi m: PGS TS Tr n Th Bách, TS Sang Mi Eum: Giám đ c Trung tâm Nghiên c u Tài nguyên sinh h c Vi t Nam-Hàn Qu c) d án, đ tài h p đ ng nghiên c u c a Vi n Sinh thái Tài nguyên sinh v t S giúp đ v tài li u m u nghiên c u c a Phòng tiêu b n th c v t - Vi n Sinh thái Tài nguyên sinh v t, Tr i h c Khoa h c t nhiên – Chí Minh, V ng i h c qu c gia Hà N i, Vi n Sinh h c nhi t đ i – Tp H n Th c v t Hoa Nam – Qu ng Châu – Trung Qu c, …., v n Qu c gia Khu b o t n thiên nhiên, n i đ n u tra nghiên c u Tôi xin trân tr ng c m n h a s Lê Kim Chi giúp hoàn thành hình v lu n án Mr Changyoung Lee, Mr Dooyoung Bae giúp đ ch p nh h t ph n hình thái h t, đ c trình t gen t i Vi n Nghiên c u Sinh h c Công ngh Sinh h c Hàn Qu c Tôi c ng xin trân tr ng c m n Ban lãnh đ o, phòng đào t o Vi n Sinh thái Tài nguyên sinh v t, H c vi n Khoa h c Công ngh t o m i u ki n t t nh t đ hoàn thành lu n án, đ c bi t s giúp đ đ ng viên c a cán b Phòng Th c v t h c su t th i gian h c t p nghiên c u M t l n n a xin đ c c m n v t t c s giúp đ Hà N i, ngày tháng Tác gi lu n án NCS V n Hài n m 2016 ii L I CAM OAN Tôi xin cam đoan s li u k t qu nêu lu n án trung th c ch a đ c công b b t k công trình khác Tác gi lu n án NCS V n Hài iii M CL C Trang L ic m n L i cam đoan M cl c Danh m c b ng Danh m c hình hình v Danh m c nh màu Danh m c b n đ B ng ký hi u vi t t t phòng tiêu b n M U 1 Tính c p thi t c a đ tài lu n án M c đích c a đ tài lu n án ụ ngh a khoa h c th c ti n c a đ tài lu n án B c c c a lu n án CH NG T NG QUAN TÀI LI U 1.1 V trí c a h Ô rô (Acanthaceae Juss.) phân tông Xuân ti t (Justiciinae) b Hoa mõm chó (Scrophulariales) l p M c lan (Magnoliopsida) ngành M c lan (Magnoliophyta) 1.2 Tình hình nghiên c u, h th ng phân lo i h ô rô (Acanthaceae) phân tông Xuân ti t (Justiciinae) 1.2.1 Trên th gi i 1.2.2 Các n c lân c n Vi t Nam CH NG 2.1 it IT Vi t Nam 13 NG, N I DUNG VÀ PH NG PHÁP NGHIểN C U 17 ng nghiên c u 17 2.2 N i dung nghiên c u 17 2.3 Ph ng pháp nghiên c u 17 2.3.1 Ph ng pháp u tra thu th p m u v t 17 2.3.2 Ph ng pháp hình thái so sánh 18 2.3.3 Ph ng pháp hình thái h t ph n 19 2.3.4 Ph ng pháp hình thái h t 22 2.3.5 Ph ng pháp đánh giá giá tr tài nguyên c a phân tông Xuân ti t 2.3.6 Ph ng pháp sinh h c phân t 23 2.3.7 Ph ng pháp k th a 23 Vi t Nam 23 CH NG K T QU NGHIểN C U 24 3.1 c m hình thái phân tông Xuân ti t (Justiciinae Nees) Vi t Nam 24 iv 3.1.1 Hình thái thân 24 3.1.2 Lá 24 3.1.3 C m hoa 25 3.1.4 Lá b c b c 25 3.1.5 Hoa 26 3.1.6 Qu 28 3.1.7 H t 28 3.2 K t qu gi i mã d li u trình t gen phân tích đ xây d ng s đ m i quan h g n g i có th gi a chi thu c phân tông Xuân ti t 29 3.3 L a ch n h th ng phân lo i phân tông Xuân ti t (Justiciinae Nees) Vi t Nam 30 3.4 Khóa đ nh lo i phân h , tông, phân tông, chi thu c h Acanthaceae Vi t Nam 34 3.4.1 Khóa đ nh lo i phân h , tông phân tông h Acanthaceae 34 3.4.2 Khóa đ nh lo i chi thu c phân tông Justiciinae Vi t Nam 34 3.5 Khóa đ nh lo i đ n loài, d (Justiciinae Juss.) i loài mô t taxon thu c phân tông xuân ti t Vi t Nam 35 3.6 Giá tr c a loài thu c phân tông xuân ti t (Justiciinae Nees) Vi t Nam 142 3.6.1 Giá tr khoa h c 142 3.6.2 Giá tr s d ng 143 3.7 M t s nh n xét th o lu n v m i quan h , xu h ng ti n hóa c a taxon phân tông Xuân ti t (Justiciinae) 144 K T LU N VÀ KI N NGH 146 NH NG I M M I C A LU N ÁN 147 DANH M C CÁC CÔNG TRỊNH CÔNG B C A TÁC GI (Có liên quan đ n công trình này) 148 TÀI LI U THAM KH O 150 B NG TRA C U TÊN KHOA H C B NG TRA C U TÊN VI T NAM PH L C Ph l c 1: nh màu đ c m hình thái loài c a phân tông Xuân ti t Vi t Nam Ph l c 2: Danh sách loài nghiên c u hình thái h t ph n hình thái h t Ph l c 3: B n đ phân b loài thu c chi c a phân tông Xuân ti t ậ H Ô rô Vi t Nam Ph l c 4: Danh sách loài gi i mã trình t gen d li u trình t gen (ITS) v DANH M C CÁC B NG Trang B ng 1.1 M t s quan m v v trí c a h Ô rô (Acanthaceae) b Hoa mõm chó (Scrophulariales) phân l p thu c l p M c lan (Manoliophyta) B ng 1.2 M t s h th ng phân chia thành tông (Trib.) phân tông (Subtrib.) B ng 1.3 M t s h th ng phân chia thành phân h (Subfam.) 11 B ng 1.4 H th ng phân lo i h Acanthaceae ông D ng theo R Benoist (1935) B ng 2.1 M i liên h c a tr c c c xích đ o v i hình d ng h t ph n v trí xích đ o (theo G Erdtman, 1952) B ng 3.1 Các taxon h Ô rô (Acanthaceae) Vi t Nam đ cs px p theo h th ng c a R W Scotland & K Vollesen (2000) B ng 3.2 H th ng phân lo i phân tông Xuân ti t (Justiciinae Nees) Vi t Nam theo h th ng c a R W Scotland & K Vollesen (2000) B ng 3.3 Danh l c loài có giá tr s (Justiciinae) Vi t Nam dung c a phân tông Xuân ti t 15 21 31 33 143 vi DANH M C HÌNH VÀ HÌNH V Hình 2.1: Thang kích th c c a h t ph n Hình 3.1: Hình d ng m t s loài thu c phân tông Justiciinae Vi t Nam Hình 3.2: Hình d ng c m hoa s loài thu c phân tông Justiciinae Vi t Nam Hình 3.3: Hình d ng b c, b c m t s loài thu c phân tông Justiciinae Vi t Nam Hình 3.4: Hình d ng đài m t s loài thu c phân tông Justiciinae Vi t Nam Hình 3.5: Hình d ng tràng m t s loài thu c phân tông Justiciinae Hình 3.6: Hình d ng nh m t s loài thu c phân tông Justiciinae Vi t Nam Vi t Nam Hình 3.7: Hình d ng nh y m t s loài thu c phân tông Justiciinae Vi t Nam Hình 3.8: Hình d ng qu m t s loài thu c phân tông Justiciinae Vi t Nam Hình 3.9: Hình d ng h t m t s loài thu c phân tông Justiciinae Vi t Nam Hình 3.10 Asystasia neesiana (Wall.) Nees Hình 3.11: Asystasia gangetica (L.) T Anders Hình 3.12: Asystasia nemorum Nees Hình 3.13: Pseuderanthemum crenulatum (Lindl.) Radlk Hình 3.14: Pseuderanthemum polyanthum (C B Clarke ex Oliver) Merr Hình 3.15: Pseuderanthemum carruthersii (Seem.) Guillaumin Hình 3.16: Pseuderanthemum bracteatum Imlay Hình 3.17: Pseuderanthemum latifolium (Vahl) B Hansen Hình 3.18: Pseuderanthemum eberhardtii Benoist Hình 3.19: Pseuderanthemum tonkinense Benoist Hình 3.20: Pseuderanthemum poilanei Benoist Hình 3.21: Codonacanthus pauciflorus (Nees) Nees Hình 3.22: Cosmianthemum knoxiifolium (C B Clarke) B Hansen Hình 3.23: Clinacanthus nutans (Burm f.) Lindau Hình 3.24: Hypoestes malaccensis Wight Hình 3.25: Hypoestes poilanei Benoist Hình 3.26: Graptophyllum pictum (L.) Griff Hình 3.27: Dicliptera vestita Benoist Hình 3.28: Dicliptera bupleuroides Nees Hình 3.29: Dicliptera leonotis Dalz ex C B Clarke Hình 3.30: Dicliptera chinensis (L.) Nees Hình 3.31: Rungia evrardii Benoist vii Hình 3.32: Rungia salaccensis Koord & Valet Hình 3.33: Rungia khasiana T Anders Hình 3.34: Rungia sarmentosa Valeton Hình 3.35: Rungia yunnanensis H S Lo Hình 3.36: Rungia chinensis Benth Hình 3.37: Rungia pectinata (L.) Nees Hình 3.38: Rungia pierrei Benoist Hình 3.39: Rungia daklakensis D.V Hai, Y.F Deng & J.K Lee Hình 3.40: Rungia clauda (Benoist) B Hansen Hình 3.41: Rungia monetaria (Benoist) B.Hansen Hình 3.42: Rungia eberhardtii (Benoist) B Hansen Hình 3.43: Pachystachys lutea Nees Hình 3.44: Ecbolium ligustrinum (Vahl) Vollesen Hình 3.45: Ptyssiglottis kunthiana (Nees) B Hansen Hình 3.46: Rhinacanthus calcaratus (Wall.) Nees Hình 3.47: Rhinacanthus nasutus (L.) Kurz Hình 3.48: Justicia neolinearifolia N H Xia & Y F Deng Hình 3.49: Justicia procumbens L Hình 3.50: Justicia diffusa Willd Hình 3.51: Justicia brandegeana Wassk & L B Smith Hình 3.52: Justicia ventricosa Wall Hình 3.53: Justicia neesiana (Nees) T Anders Hình 3.54: Justicia alboviridis Benoist Hình 3.55: Justicia quadrifaria (Nees) T Anders Hình 3.56: Justicia grossa C B Clarke Hình 3.57: Justicia amherstia Bennet Hình 3.58: Justicia gendarussa Burm f Hình 3.59: Justicia prominens Benoist Hình 3.60: Justicia aequalis Benoist Hình 3.61: Justicia comata (L.) Lam Hình 3.62: Justicia carnea Lindl Hình 3.63: Justicia glabra Koenig ex Roxb Hình 3.64: Justicia vagabunda Benoist Hình 3.65: Justicia adhatoda L Hình 3.66: Justicia cochinchinensis Benoist viii Hình 3.67: Justicia oreophila C B Clarke Hình 3.68: Justicia panduriformis Benoist Hình 3.69: Justicia cardiophylla D Fang & H S Lo Hình 3.70: Justicia glomerulata Benoist Hình 3.71: Justicia leptostachya Hemsl Hình 3.72: Justicia myuros Benoist Hình 3.73: Justicia patentiflora Hemsl Hình 3.74: Justicia poilanei Benoist Hình 3.75: Justicia candida Benoist Hình 3.76: Justicia kampotiana Benoist Hình 3.77: Justicia ingrata Benoist Hình 3.78: Isoglossa clemensorum (Benoist) B Hansen Hình 3.79: Isoglossa fastidiosa (Benoist) B Hansen Hình 3.80: Isoglossa inermis (Benoist) B Hansen Hình 3.81: Isoglossa collina (T Anders.) B Hansen Hình 3.82: Cyclacanthus coccineus S Moore Hình 3.83: Cyclacanthus poilanei Benoist Hình 3.84: Peristrophe lanceolaria (Roxb.) Nees Hình 3.85: Peristrophe paniculata (Forsk.) Brumitt Hình 3.86: Peristrophe magnibracteata (Collett & Hemsl.) Z P Hao, Hình 3.87: Peristrophe japonica (Thunb.) Bremek Hình 3.88: Peristrophe bivalvis (L.) Merr Hình 3.89: Peristrophe montana (Wall.) Nees Hình 3.90: Peristrophe acuminata Nees B n đ 3.26 B n đ phân b loài thu c chi Cyclacanthus hi n bi t  Cyclacanthus coccineus S Moore  Cyclacanthus poilanei Benoist Vi t Nam B n đ 3.27 B n đ phân b loài thu c chi Peristrophe hi n bi t Vi t Nam  Peristrophe lanceolaria (Roxb.) Nees  Peristrophe magnibracteata (Collett & Hemsl.) Z P Hao, Y F Deng & N H Xia  Peristrophe japonica (Thunb.) Bremek B n đ 3.28 B n đ phân b loài thu c chi Peristrophe hi n bi t  Peristrophe bivalvis (L.) Merr  Peristrophe montana (Wall.) Nees  Peristrophe acuminata Nees Vi t Nam i Ph l c 4: DANH SÁCH CÁC LOÀI GI I MÃ TRÌNH T VÀ D LI U TRÌNH T GEN (ITS) Stt Tên khoa h c Asystasia gangetica (L.) T Anders D li u l yt genbank Mã s genbank x KP744316.1 GEN Mã s gen phân tích x Loài có Vi t Nam x x Asystasia nemorum Nees KT004469.1 Clinacanthus nutans (Burm f.) Lindau Codonacanthus pauciflorus (Nees) Nees Cyclacanthus poilanei Benoist DVH Dicliptera chinensis (L.) Nees Sine num Dicliptera bupleuroides Nees PTV 380 Ecbolium ligustrinum (Vahl) Vollesen Graptophyllum pictum (L.) Griff 10 Hypoestes aristata (Vahl) Roem & Schult 11 Isoglossa clemensorum (Benoist) B Hansen DVH 04 12 DVH 17 13 Isoglossa inermis (Benoist) B Hansen Justicia amherstia Bennet [Justicia fragilis Wall.] Sine num 14 Justicia gendarussa Burm f Sine num 15 Justicia glomerulata Benoist DVH 26 16 Justicia myuros Benoist Cuong 313 17 Justicia poilanei Benoist HG 602 18 Justicia ventricosa Wall DVH 112 19 Pachystachys lutea Nees 20 Peristrophe bivalvis (L.) Merr 21 Pseuderanthemum eberhardtii Benoist 22 Pseuderanthemum poilanei Benoist Pseuderanthemum polyanthum (C B Clarke ex Oliver) Merr x x KP744318.1 x Sine num x x x x x EU087476.1 x PTV 728 x AF289765.1 x x x x x x x x x x KP744340.1 C x x DVH 24 x 23 DVH 23 Hài 26022014-2 x ii x 24 Ptyssiglottis pubisepala (Lindau) B.Hansen AF289787.1 x x 25 Rhinacanthus nasutus (L.) Kurz KP744345.1 26 Rungia eberhardtii (Benoist) B Hansen 27 Rungia evrardii Benoist CTTNB 61 28 Rungia pectinata (L.) Nees Sine num 29 Rungia pierrei R Ben DVH 19 30 Rungia sarmentosa Valeton DVH 01 31 Rungia clauda Benoist PTV 1033 32 Thunbergia coccinea Wall PTV 691 HL-VB (DVH) x x x x x x x iii Dicliptera_chinensis Hypoestes_aristata Peristrophe_bivalvis Dicliptera_bupleuroides Rhinacanthus_nasutus Cyclacanthus_poilanei Clinacanthus_nutans Isoglossa_clemensorum Isoglossa_inermis Pachystachys_lutea Ecbolium_ligustrinum Pseuderanthemum_poilanei Pseuderanthemum_polyanthum Graptophyllum_pictum Pseuderanthemum_eberhardtii Codonacanthus_pauciflorus Ptyssiglottis_pubisepala Rungia_pierrei Rungia_sarmentosa Rungia_evrardii Rungia_pectinata Rungia_eberhardtii Rungia_clauda Justicia_fragilis Justicia_gendarussa Justicia_ventricosa Justicia_myuros Justicia_poilanei Justicia_glomerulata Asystasia_gangetica Asystasia_nemorum Thunbergia_coccinea -TTTCCAG -CTTATCATTTAGAGGAAGGAGAAGTCGTAACAAGGTTTCCG ACCTTATCATTTAGAGGAAGGAGAAGTCGTAACAAGGTTTCCG -CACTGGGACCTTATCATTTAGAGGAAGGAGAAGTCGTAACAAGGTTTCCG -ACCTTATCATTTAGAGGAAGGAGAAGTCGTAACAAGGTTTCCG CTATCATTTAGAGGAAGGAGAAGTCGTAACAAGGTTTCCG CTATCATTTAGAGGAAGGAGAAGTCGTAACAAGGTTTCCG -TTCCG -TTAGAGGAAGGAGAAGTCGTAACAAGGTTTCCG -TATCATTTAGAGGAAGGAGAAGTCGTAACAAGGTTTCCG CTTATCATTTAGAGGAAGGAGAAGTCGTAACAAGGTTTCCG -CTTATCATTTAGAGGAAGGAGAAGTCGTAACAAGGTTTCCG -CTTATCATTTAGAGGAAGGAGAAGTCGTAACAAGGTTTCCG -CTTATCATTTAGAGGAAGGAGAAGTCGTAACAAGGTTTCCG -CTTATCATTTAGAGGAAGGAGAAGTCGTAACAAGGTTTCCG -CTTATCATTTAGAGGAAGGAGAAGTCGTAACAAGGTTTCCG -CTTATCATTTAGAGGAAGGAGAAGTCGTAACAAGGTTTCCG -CTTATCATTTAGAGGAAGGAGAAGTCGTAACAAGGTTTCCG -CTTATCATTTAGAGGAAGGAGAAGTCGTAACAAGGTTTCCG -TATCATTTAGAGGAAGGAGAAGTCGTAACAAGGTTTCCG -CTTATCATTTAGAGGAAGGAGAAGTCGTAACAAGGTTTCCG CTATCATTTAGAGGAAGGAGAAGTCGTAACAAGGTTTCCG -TTCCG Dicliptera_chinensis Hypoestes_aristata Peristrophe_bivalvis Dicliptera_bupleuroides Rhinacanthus_nasutus Cyclacanthus_poilanei Clinacanthus_nutans Isoglossa_clemensorum Isoglossa_inermis Pachystachys_lutea Ecbolium_ligustrinum Pseuderanthemum_poilanei Pseuderanthemum_polyanthum Graptophyllum_pictum Pseuderanthemum_eberhardtii Codonacanthus_pauciflorus Ptyssiglottis_pubisepala Rungia_pierrei Rungia_sarmentosa Rungia_evrardii Rungia_pectinata Rungia_eberhardtii Rungia_clauda Justicia_fragilis Justicia_gendarussa Justicia_ventricosa Justicia_myuros Justicia_poilanei Justicia_glomerulata Asystasia_gangetica Asystasia_nemorum Thunbergia_coccinea TAGGTGAACCTGCGGAAGGATCATTGTCGATACCTGC-GAGGCAGACC-G -AA-GGAGAGCACC-G CCTGC-GAGGCAGACC-G TAGGTGAACCTGCGGAAGGATCATTGTCGATACCTGC-GAGGCAGACC-G TCGATACCTGC-GAGGCAGACCCG TAGGTGAACCTGCGGAAGGATCATTGTCGAGACCTGC-AAGGCAGACCCG TCGAGACCTGC-AAGGCAGACC-G TAGGTGAACCTGCGGAAGGATCATTGTCGAGACCTGC-AAGGCAGACC-G TAGGTGAACCTGCGGAAGGATCATTGTCGAGACCTGC-AAGGCAGACC-G TCGAGACCTGC-AAGGCAGACC-G -ATGTGAACCTGCGGAAGGATCATTGTCGAGACCTGC-AAGGCAGACC-G TAGGTGAACCTGCGGAAGGATCATTGTCGAAACCTGCGAAGGCAGACC-G TAGGTGAACCTGCGGAAGGATCATTGTCGAAACCTGCGAAGGCAGACC-G TAGGTGAACCTGCGGAAGGATCATTGTCGAAACCTGCGAAGGCAGACC-G TAGGTGAACCTGCGGAAGGATCATTGTCGAAACCTGCGAAGGCAGACC-G TAGGTGAACCTGCGGAAGGATCATTGTCGAAACCTGCGAAGGCAGACC-G -GAACCTGCGGAAGGATCATTGTCGAAACCTGC-AAGGCAGACC-G TAGGTGAACCTGCGGAAGGATCATTGTCGAAACCTGC-AAGGCAGACC-G TAGGTGAACCTGCGGAAGGATCATTGTCGAAACCTGC-AAGGCAGACC-G TAGGTGAACCTGCGGAAGGATCATTGTCGAAACCTGC-AAGGCAGACC-G TAGGTGAACCTGCGGAAGGATCATTGTCGAAACCTGC-AAGGCAGACC-G TAGGTGAACCTGCGGAAGGATCATTGTCGAAACCTGC-AAGGCAGACC-G TAGGTGAACCTGCGGAAGGATCATTGTCGAAACCTGC-AAGGCACACC-G TAGGTGAACCTGCGGAAGGATCATTGTCGAAACCTGC-AAGGCAGACC-G TAGGTGAACCTGCGGAAGGATCATTGTCGAAACCTGC-AAGGCAGACC-G TAGGTGAACCTGCGGAAGGATCATTGTCGAAACCTGC-AAGGCAGACC-G TAGGTGAACCTGCGGAAGGATCATTGTCGAGACCTGC-GAGGCAGACC-G TAGGTGAACCTGCGGAAGGATCATTGTCGAGACCTGC-AAGGCAGACC-G TAGGTGAACCTGCGGAAGGATCATTGTCGAGACCTGC-AAGGCAGACC-G -TTGTCGATACCTGA-TAGGCAGACC-G -TAGGTGAACCTGCGGAAGGATCATTGTCGAAACCTGC-AAAGCAGACC-G iv Dicliptera_chinensis Hypoestes_aristata Peristrophe_bivalvis Dicliptera_bupleuroides Rhinacanthus_nasutus Cyclacanthus_poilanei Clinacanthus_nutans Isoglossa_clemensorum Isoglossa_inermis Pachystachys_lutea Ecbolium_ligustrinum Pseuderanthemum_poilanei Pseuderanthemum_polyanthum Graptophyllum_pictum Pseuderanthemum_eberhardtii Codonacanthus_pauciflorus Ptyssiglottis_pubisepala Rungia_pierrei Rungia_sarmentosa Rungia_evrardii Rungia_pectinata Rungia_eberhardtii Rungia_clauda Justicia_fragilis Justicia_gendarussa Justicia_ventricosa Justicia_myuros Justicia_poilanei Justicia_glomerulata Asystasia_gangetica Asystasia_nemorum Thunbergia_coccinea CGAACACGTGCT CAACCGCGCCGCGGGCGGGGAGCGGGTG -CGAACACGTGCTATTCTACGTCGTCGCCGTGGTCGGGGAGCTGGTGGGAT CGAACACGTGCT CAACGCCGCCGCGGGCGGGGAGCGGGGG -TGAACACGTGCT CAACGCCGCCGCGGACGGGGAGCTGGGAC CGAACTCGTGAT CAACACCGCCGCGGTCCCGG -CGAACGCGTGCC -TAA-CAC -C CGTCGTGG -CGAACGCGTGCC -TAAACAC -C CGTCGCGG -CGAACGCGTGCC -TAA-CAC -C CGTCGCGG -CGAACGCGTGCC -TAA-CAC -C CGTCGCGG -CGAACGCGTGCT -CAA-CGC -AGGCGCCGCGG -CGAACGCGTGCT -CAA-CGC -CGGCGCCGTGG -CGAACACGTGTT -TAACCTT -GTCGAGGCTCCGGTGCGGGGG -TGAACACGTGTT -TAACCTT -GTCGAGGCTCCGGTGCGGGGG -CGAACACGTGTT -TAACCTT -GTCGAGGCTCCGGTGCGGGGG -CGAACACGTGTT -TAACCTT -GTCGAGGCTCCGGTGCGGGGG -CGAACATGTGTT -TAACCCT -GTCGGGGCTCCGGTGCGGGGG -CGAACACGTGTT -AAACCCC -GTCGGGGCCGTGGTGCGGGGG -CGAACACGTGTT -TAACCTC -GTCGCGTGTCCGGTGCGGGGG -CGAACACGTGTT -TAACCCC -GTCGCGTGTCCGGTGCGGCGG -CGAACACGTGTT -TAACAGTCGGGCCGCGCGTCGGGTTCGGGGG -CGAACACGTGTT -TAACCGTCGGGTCGCGCGTCGGGTTCGGGGG -CGAACACGTGTT -TAACCTC -GTCGCGGATCGGGTGCGGGTG -CGAACACGTGTT -TAACCTC -GTCGCGGATCGGGTGCGGGGG -CGAACACGTGTT -TAACCTC -GTCGCGGATCCGGTGTGGGGGG TGAACACGTGTT -TAACCTC -GTCGCGGATCCGGTGTGGGGGG CGAACACGTGTT -TAACCTC -GTCGCGGATCCGGTGCGGGGGA CGAACACGTGTT -TAACCTC -GTCGTGAAACCGGTGCGGGTGG CGAACACGTGTT -TAACCCC -GTCGCGGAACCGGTGCGGGTGG TGAACACGTGTT -TAACCTC -GTCGCGGACCCGGTGCGGGTGG CGAACACGTTCG -GCCGC GGGGCGGGGG CGAACACGTTCC CGACAC -ATCGGGGCCTCGGCGTGGGGTG - Dicliptera_chinensis Hypoestes_aristata Peristrophe_bivalvis Dicliptera_bupleuroides Rhinacanthus_nasutus Cyclacanthus_poilanei Clinacanthus_nutans Isoglossa_clemensorum Isoglossa_inermis Pachystachys_lutea Ecbolium_ligustrinum Pseuderanthemum_poilanei Pseuderanthemum_polyanthum Graptophyllum_pictum Pseuderanthemum_eberhardtii Codonacanthus_pauciflorus Ptyssiglottis_pubisepala Rungia_pierrei Rungia_sarmentosa Rungia_evrardii Rungia_pectinata Rungia_eberhardtii Rungia_clauda Justicia_fragilis Justicia_gendarussa Justicia_ventricosa Justicia_myuros Justicia_poilanei Justicia_glomerulata Asystasia_gangetica Asystasia_nemorum Thunbergia_coccinea -TGCCTACACCCCC TCCGCGTCCGCGGCAAC CCCCC GG CTTTGATTGGTCCCCCGCTCCGCGTCCGTGGCGACGACCCCCCCGCCGGA CGCGTCCCCCCCTCCGCGCCCGCGGCAAC CCCCCC -GC -TTAGCGTCCCTCCTCCGCGTCCGTGGCACC CCCCC GA CCCCG CCGAGGCCGCGGCGCC CCGCCG -GC -CTCCGGCCGCGACGCT CC CCCCGCC GG -CTCCGGCCGCGACGTCTCCC CCCCGCC GG -CTCCGGCCGCGACGTC CC CCCCGCC GG -CTCCGGCCGCGACGTC CC CCCCGCC GG -CTCCGGCCGCGACGCCTGCC CCCCGCG CTCCGGCCGCGGCGCTGCCC CCCCTCC C -CGTGTCCCCTCTCCGTCGTCCCGAC -TCCCGCC GG -CGTGTCCCCCCTCCGTCGTCCCGAT -CCCCGCC GG -CGTGTCCCCCCTCCGTCGTCTTGAC -CCCCGCC GG -CGTGTCCCCTCTCCGTTGCCCCGAC -CCCCGCC AG -CGMGTCCCCCCTCCGTCRTCCCGGC -CCCCGCC GG -CGTGCCCCCCCGCCGCGGCTGCGGCGTC CCCCGCC AG CACGACGTGTCCCCGCACCGCGGCCGTGACC CCCCGCC GG CACGAAGCGTCCCCGCACCGCGGCCGTGACC CCCCGCC GG CACGACCTGCCCCCGC-TCACGGCCGCGACCT -CCCCGCC GG CACGACCTGCCCCCGC-TCGCGGCCGCGACCT -CCCCGCA GG CACGACGTGCCCCCGT-ACGTGGCCGCGACCC -CCCCGCC GG CACGACGTGCCCCCGT-CCGCGGCCGCGACCC -CCCCGCC GG CACGACGTGTCCCTCT-CCTCGGCCGCGACC CCCCATC GG CACGACGTGTCCCTCT-CCTCGGCCGCGACC CCCCATC GG CACGACGTGTCCCTCT-CCTCGGCCGCAACC CCCCGTC GG CACGTCCGTGTCCCC-GCGTCGGCCGCGGCCC -CCCCGTC GG CACGTCCGTGTCCCCCGTGTTGGTCGCGGCCT -CCTCGCC GG CACGTCCGTGTCCCCTGCGTCGACCGTGGCTA -CCCCATC GG -CTCGTCCCCCGCCCCGAACCCCGCCCGCGGCG -CG -CCCCCTCCCGCCGTTCC CCTCCCC -G v Dicliptera_chinensis Hypoestes_aristata Peristrophe_bivalvis Dicliptera_bupleuroides Rhinacanthus_nasutus Cyclacanthus_poilanei Clinacanthus_nutans Isoglossa_clemensorum Isoglossa_inermis Pachystachys_lutea Ecbolium_ligustrinum Pseuderanthemum_poilanei Pseuderanthemum_polyanthum Graptophyllum_pictum Pseuderanthemum_eberhardtii Codonacanthus_pauciflorus Ptyssiglottis_pubisepala Rungia_pierrei Rungia_sarmentosa Rungia_evrardii Rungia_pectinata Rungia_eberhardtii Rungia_clauda Justicia_fragilis Justicia_gendarussa Justicia_ventricosa Justicia_myuros Justicia_poilanei Justicia_glomerulata Asystasia_gangetica Asystasia_nemorum Thunbergia_coccinea CGGCGCTCTCGC -GTCGTGCGGGGCTAACTAACGAACCCCGGCGCG CGGCGCTCTCGC -GTCGTGCGTGCGGGTTTAACGAACCCGGGCGCG CGGCGCTCTCGC -GCCGCGCG GGGCTAACGAACCCCGGCGCG CGGCGCTCTTGC -GTCGTGCG GGGCTAACGAACCCCGGCGCG GAGCGCTCCCGCT -CGCCGTGCG -GGCTAACGAACCCCGGCGCG CC -TGCGCC -GCGGGGG G-C-CAACGAACCCCGGCGCA CC -TGCGCC -GCGGGGG GGC-CAACGAACCCCGGCGCG CC -TTCGCC -GCGGGGG G-C-CAACGAACCCCGGCGCG CC -TTCGCC -GCGGGGG G-C-CAACGAACCCCGGCGCG GTT -GCGGGGG GGC AACGAACCCCGGCGCG CG -CCCGGG -GCGGGGG GGC-CAACGAACCCCGGCGCG CG-CGCGCCTGCG-TGCGTTGTGCG GGC-TAACGAACCCCGGCGCG CG-CGCGCCTGCG-TGCGTTGTGCG GGC-TAACGAACCCCGGCGCG CG-CGCGCTTGCA-TGCGTTGTGCG GGC-TAACGAACCCCGGCGCG CG-TGCGCCTGCG-TGCGTTGTGCG GGC-TAACGAACCCCGGCGCG CG-CGCGCTCGCG-TGCGTCGTGCG GGC-TAACGAACCCCGGCGCG CG-CGCGCTCGCG-TGTGCTGTGTG GGC-TAA ACCCCGGGCGCG CG-TGCCCTTGGG-TGCGTCGTGCG GGC-TAACGAACCCCGGCGCG CG-TGCCCTCGGG-TGCGTCGTGCG GGC-TAACGAACCCCGGCGCG CGATGCCCCTGGGGCGCGCCGTGCG GGCATAACGAACCCCGGCGCG CGACGCCCCCGGG-CGCGCCGTGCG GGCATAACGAACCCCGGCGCG CG-TGCCCTCGCG-TGCGCCGTGCG GGC-TAACGAACCCCGGCGCG CG-TGCCCTTGCG-TGCGCCGTGCG GGC-TAACGAACCCCGGCGCG CG-TGCCCTCGGG-TGTGTCGTGCG GGC-TAACGAACCCCGGCGCG CG-TGCCCTCGGG-TGTGTCGTGCG GGC-TAACGAACCCCGGCGCG CG-TGCCCTCGGG-TGTGTCGTGCG GGC-TAACGAACCCCGGCGCG CG-TGCCCTTGGG-CGCGCCGTGCG GGC-TAACTAACCCCGGCGCG CG-TGCCCGAGGG-CACGCCGTGCG GGC-TAACGAACCCCGGCGCG CG-TGCCCTCGGG-TGCGTTGTGCG GGC-TAATGAACCCCGGCGCG CG-ATCGCTCGCGCGTCGTCGTGYG GGC-TAACGAACCCCGGCGCG -CGGGGCGCGCGCG AGCGTGCGCCTCGTGGGCTAACGAACCCCGGCGCG Dicliptera_chinensis Hypoestes_aristata Peristrophe_bivalvis Dicliptera_bupleuroides Rhinacanthus_nasutus Cyclacanthus_poilanei Clinacanthus_nutans Isoglossa_clemensorum Isoglossa_inermis Pachystachys_lutea Ecbolium_ligustrinum Pseuderanthemum_poilanei Pseuderanthemum_polyanthum Graptophyllum_pictum Pseuderanthemum_eberhardtii Codonacanthus_pauciflorus Ptyssiglottis_pubisepala Rungia_pierrei Rungia_sarmentosa Rungia_evrardii Rungia_pectinata Rungia_eberhardtii Rungia_clauda Justicia_fragilis Justicia_gendarussa Justicia_ventricosa Justicia_myuros Justicia_poilanei Justicia_glomerulata Asystasia_gangetica Asystasia_nemorum Thunbergia_coccinea GAACGCGCCAAGGAAAACCG -AAACGAGGCGCCCTCCCCCC-GTGC GAATGCGCCAAGGAAAACTG -AAACGGAGCGCCCTCCTCCC-GTCC GAACGCGCCAAGGAAAACCG -AAACGAGGCGCCCTCCCCCCCGTCC GAATGCGCCAAGGAAAACCG -AAACGACGCGCCCTCTCCAC-GTCC GAACGCGCCAAGGAAAACGG -TAACGGAGCGCCCTCCGCCC-GGCC GAACGCGCCAAGGAAAACCG -AAACGAAGCGCCCTCCC-CC-GTCC GAACGCGCCAAGGAAAACCG -AAACGAAGCGCCCTCCC-CC-GTCC GAACGCGCCAAGGAAAACTG -AAACGAAGCGCCCTCCC-CC-GTCC GAACGCGCCAAGGAAAACTG -AAACGAAGCGCCCTCCC-CC-GTCC GAACGCGCCAAGGAAAACCG -AAACGAAGCGCCCTCCC-CC-GTCC GAACGCGCCAAGGAAAACCG -AAACGAAGCGCCCTCCC-CC-GTCC GAACGCGCCAAGGAATACCG -AAACGAAGCGCCCTCCC-CT-GCTC GAAAGCGCCAAGGAATACCG -AAACGAAGCGCCCTCCC-CT-GCTC GAACGCGCCAAGGAATACCG -AAACGAAGCACCCTCCC-CT-GCTC GAACGCGCCAAGGAATACCG -AAACGAAGCGCCCTCCC-CT-GCTC GAATGCGCCAAGGAATACTG -TAACGAAGCGCCCTCCC-CT-GCTC GAACGCGCCAAGGAAAACCG -AAACGAAGCGCCCTCCCTCC-GCCC GAAAGCGCCAAGGAAAACTG AAAAAACGACGCGCCCTCCC-CC-GTCC GAAAGCGCCAAGGAGAACCG AAAAAGAGACGTGCCCTCCC-CC-GTCC GAAAGCGCCAAGGAAAACCG-AAAAAAACGATGCGCCCTCCC-CT-GCCC GAAAGCGCCAAGGAAAACCGTAAAAAAACGATGCGCCCTCCC-CC-GTCC GAAAGCGCCAAGGAAAACCG -AAAAACGATGCGACCTCCC-CC-GTCC GAAAGCGCCAAGGAAAACCG -AAAAACGATGCGCCCTCCC-CC-GTCC GAAAGCGCCAAGGAAAACCG -AAAAACGATGCGCCCGCCC-CC-GTCC GAAAGCGCCAAGGAAAACCG -AAAAACGATGCGCCCGCCC-CC-GTCC GAAAGCGCCAAGGAAAACCG -AAAAACGATGCGCCCGCCC-CC-GTCC GAAAGCGCCAAGGAAAACAA A-TGAACGACGCGCCCACCC-CC-GTCC GAAAGCGCCAAGGAAAACAA AACGAATGAYGCACCCGCCC-CC-GTCC GAAAGCGCCAAGGAAAATGG AAAGAACGATGCGCCCGCCC-AC-GTCC GAACGCGCCAAGGAATACCGAAACGAAGCGCCGTCCCCGCCCCCCCGCTC -GGATGCGCCAAGGAAAACAG AAAACGAAACGCCNCCGCACC -CC vi Dicliptera_chinensis Hypoestes_aristata Peristrophe_bivalvis Dicliptera_bupleuroides Rhinacanthus_nasutus Cyclacanthus_poilanei Clinacanthus_nutans Isoglossa_clemensorum Isoglossa_inermis Pachystachys_lutea Ecbolium_ligustrinum Pseuderanthemum_poilanei Pseuderanthemum_polyanthum Graptophyllum_pictum Pseuderanthemum_eberhardtii Codonacanthus_pauciflorus Ptyssiglottis_pubisepala Rungia_pierrei Rungia_sarmentosa Rungia_evrardii Rungia_pectinata Rungia_eberhardtii Rungia_clauda Justicia_fragilis Justicia_gendarussa Justicia_ventricosa Justicia_myuros Justicia_poilanei Justicia_glomerulata Asystasia_gangetica Asystasia_nemorum Thunbergia_coccinea GAGCCCCGTCCGCGG-GCGCTCCGGACGGGCGGGCAGGATGCGC AT GAGCCCCGTCCGCGGCGTGCTATGGACGGGCGGAAAGGATGCGCCTCAAT GAGCCCCGTCCGCGGCGCGCTGCGGACGGGCGGGAAGGATGCGC AT GAGCCCCGTTCGCGGAGCGCTGCGGACGGGCGGGCAGGATGCGC AT GAGCCCCGTCCGCGGTGCGCCACGGACGGGCGGAAGGGCTGCGC CT GTGCCCCGTTCGCGGTGCGCC-CGCGCGGGGGGAAAGGCCGCGC CT GTGCCCCGTTCGCGGTGCGCC-TGGGCGGGGGGAA-GGCCGCGC CT GTGCCCCGTTCGCGGTGCGCC-CGGGTGGGGGGAA-GGCCGCGC CT GTGCCCCGTTCGCGGTGCGCC-CGGGCGGGGGGAA-GGCCGCGC CT GTGCCCCGTTCGCGGTGCGCC-CGGGCGGGGGGAA-GGCTGCGC CT GTGCCCCGTTCGCGGTGCGCC-CGGGCGGGGGGAA-GGATGCGC CT GTGCCCCGTTCGCGGTGTGCC-TGGGCTGGGGGA-CGGTTGCGC CT GTGCCCCGTTCGCGGTGTGCT-TGGGCGGGGGGA-TGGTTGTGC CT GTGCCCCGTTCGCGGTGTGCC-TGGGCGGGGGGA-TGGTTGCGC CT GTGCCCCGTTCGCGGTGTGCC-TGGGTGGGGGGA-TGGTTGCGC CT GTGCCCCGTTCGCGGTGTGCC-TGGGCGGGGTGA-TGGTTGCGC CT GTGCCCCGTTCGCGGTGCGCC-CGGGTGGGGGGA-AGGTCGCGC CT GTGCCCCGTTCGCGGTGTGTC-CGGGCGCGGGGA-AGGCCGCGC GT GTGCCCCGTTCGCGGTGCGTC-CGGGCGGGGGGA-AGGCCGCGC GT GTGCCCCGTTCGCGGTGCGCC-CGGGTGCGGG-A-AGGCCGCGC GT GTGCCCCGTTCGCGGTGCGCC-CGGGCGTGGGGA-AGGCCGCGC GT GTGCCCCGTTCGCGGTGCGTC-CGGGCGCGGGGA-AGGCTGCGC GT GTGCCCCGTTCGCGGTGCGTC-CGGGCGCGGGGA-AGGCTGCGC GT GTGCCCCGTTCGCGGTGCGTC-CGGGCGTGGGGA-AGGTCGCGC GT GTGCCCCGTTCGCGGTGCGTC-CGGGCGTGGGGA-AGGTCGCGC GT GTGCCCCGTTCGCGGTGCGTC-CGGGCGTGGGGA-AGGTCGCGC GT GTGCCCCGTCCGCGGTGCGCC-CGGGCGCAGGGA-GGGCCGCGC GT GTGCCCCGTACGCGGTGCGCC-CGGGCGTAGGGGTGGGCCGCGC GT GTGCCCCGTTCGCGGTGCGCA-CGTGCGTGCGAA-GGGCCGCAC GT GTGCCCCGTCCGCGGTGCGCT-CGGGCGGGGGGA-CGGCGGCGC CT -CGGCCCCGTCCGCGGTGCGCT-CGGGTGGGTGTGCGCGGCGTCT CC Dicliptera_chinensis Hypoestes_aristata Peristrophe_bivalvis Dicliptera_bupleuroides Rhinacanthus_nasutus Cyclacanthus_poilanei Clinacanthus_nutans Isoglossa_clemensorum Isoglossa_inermis Pachystachys_lutea Ecbolium_ligustrinum Pseuderanthemum_poilanei Pseuderanthemum_polyanthum Graptophyllum_pictum Pseuderanthemum_eberhardtii Codonacanthus_pauciflorus Ptyssiglottis_pubisepala Rungia_pierrei Rungia_sarmentosa Rungia_evrardii Rungia_pectinata Rungia_eberhardtii Rungia_clauda Justicia_fragilis Justicia_gendarussa Justicia_ventricosa Justicia_myuros Justicia_poilanei Justicia_glomerulata Asystasia_gangetica Asystasia_nemorum Thunbergia_coccinea CACGTGAGA-TCTC -AAAACGACTCTCGGCAACGGATATCTAGGCTCT CGATTGAGA-TGTCTCTGAAACGACTCTCGGCAACGGATATCTAGGCTCT CACGTGAGA-TGTC -AAAACGACTCTCGGCAACGGATATCTAGGCTCT CACGTGAGA-TGTC -AAAACGACTCTCGGCAACGGATATCTAGGCTCT CGAGTGAGAACGTC -AAAACGACTCTCGGCAACGGATATCTCGGCTCT CACGTA CGTC -AAAACGACTCTCGGCAACGGATATCTCGGCTCT CACGTA CGTC -AAAACGACTCTCGGCAACGGATATCTCGGCTCT CACGTA CGTT -AAAACGACTCTCGGCAACGGATATCTCGGCTCT CACGTA CGTC -AAAACGACTCTCGGCAACGGATATCTCGGCTCT CACGTAT-G-TGTC -AAAACGACTCTCGGCAACGGATATCTCGGCTCT CACGTA A-AGTC -GAAACGACTCTCGGCAACGGATATCTCGGCTCT CCT -TTA-TGTC -AAAACGACTCTCGGCAACGGATATCTCGGCTCT CCT -TTA-TGTC -AAAACGACTCTCGGCAACGGATATCTCGGCTCT TCT -TTA-TGTC -AAAACGACTCTCGGCAAYGGATATCTCGGCTCT CCT -T-A-TGTC -AAAACGACTCTCGGCAACGGATATCTCGGCTCT CTT -TTA-TGTC -AAAACGACTCTCGGCAACGGATATCTCGGCTCT CAC -GTA-TGTC -AAAACGACTCTCGGCAACGGATATCTCGGCTCT CATGAACGA-TGTC -AAAACGACTCTCGGCAACGGATATCTCGGCTCT CGTGAACGA-TGTC -AAAACGACTCTCGGCAACGGATATCTCGGCTCT CATGAACAA-TGTC -AAAATGACTCTCGGCAACGGATATCTCGGCTCT CATGAACGA-TGTC -AAAACGACTCTCGGCAACGGATATCTCGGCTCT CATGAACGA-TGTC -AAAACGACTCTCGGCAACGGATATCTCGGCTCT CATGAACGA-TGTC -AAAACGACTCTCGGCAACGGATATCTCGGCTCT CATGAACGA-TGTC -AAAACGACTCTCGGCAACGGATATCTCGGCTCT CATGAACGA-TGTC -AAAACGACTCTCGGCAACGGATATCTCGGCTCT CATGAACGA-TGTC -AAAACGACTCTCGGCAACGGATATCTCGGCTCT CACGAACGA-TGTC -TAAACGACTCTCGGCAACGGATATCTCGGCTCT CACGAAYGA-TGTC -AAAACGACTCTCGGCAACGGATATCTCGGCTCT CATGAATGA-TGTC -AAAATGACTCTCGGCAACGGATATCTCGGCTCT CACCTA CGTC -GAAACGACTCTCGGCAACGGATATCTCGGCTCT -CAC -AATGTC -TAAACGACTCTCGGCAACGGATATCTCGGCTCT vii Dicliptera_chinensis Hypoestes_aristata Peristrophe_bivalvis Dicliptera_bupleuroides Rhinacanthus_nasutus Cyclacanthus_poilanei Clinacanthus_nutans Isoglossa_clemensorum Isoglossa_inermis Pachystachys_lutea Ecbolium_ligustrinum Pseuderanthemum_poilanei Pseuderanthemum_polyanthum Graptophyllum_pictum Pseuderanthemum_eberhardtii Codonacanthus_pauciflorus Ptyssiglottis_pubisepala Rungia_pierrei Rungia_sarmentosa Rungia_evrardii Rungia_pectinata Rungia_eberhardtii Rungia_clauda Justicia_fragilis Justicia_gendarussa Justicia_ventricosa Justicia_myuros Justicia_poilanei Justicia_glomerulata Asystasia_gangetica Asystasia_nemorum Thunbergia_coccinea CGCATCGATGAAGAACGTAGCAAAATGCGATACTTGGTGTGAATTGCAGA CGCATCGATGAAGAACGTAGCAAAATGCGATACTTGGTGTGAATTGCAGA CGCATCGATGAAGAACGTAGCAAAATGCGATACTTGGTGTGAATTGCAGA CGCATCGATGAAGAACGTAGCAAAATGCGATACTTGGTGTGAATTGCAGA CGCATCGATGAAGAACGTAGCGAAATGCGATACTTGGTGTGAATTGCAGA CGCATCGATGAAGAACGTAGCGAAATGCGATACTTGGTGTGAATTGCAGA CGCATCGATGAAGAACGTAGCGAAATGCGATACTTGGTGTGAATTGCAGA CGCATCGATGAAGAACGTAGCGAAATGCGATACTTGGTGTGAATTGCAGA CGCATCGATGAAGAACGTAGCGAAATGCGATACTTGGTGTGAATTGCAGA CGCATCGATGAAGAACGCAGCGAAATGCGATACTTGGTGTGAATTGCAGA CGCATCGATGAAGAACGCAGCNNNNNNNNNNNNNNNNNNNNNNNNNNNNN CGCATCGATGAAGAACGTAGCGAAATGCGATACTTGGTGTGAATTGCAGA CGCATCGATGAAGAACGTAGCGAAATGCGATACTTGGTGTGAATTGCAGA CGCATCGATGAAGAACGTAGCGAAATGCGATACTTGGTGTGAATTGCAGA CGCATCGATGAAGAACGTAGCGAAATGCGATACTTGGTGTGAATTGCAGA CGCATCGATGAAGAACGTAGCGAAATGCGATACTTGGTGTGAATTGCAGA CGCATCGATGAAGAACGTAGCGAAATGCGATACTTGGTGTGAATTGCAGG CGCATCGATGAAGAACGTAGCGAAATGCGATACTTGGTGTGAATTGCAGA CGCATCGATGAAGAACGTAGCGAAATGCGATACTTGGTGTGAATTGCAGA CGCATCGATGAAGAACGTAGCGAAATGCGATACTTGGTGTGAATTGCAGA CGCATCGATGAAGAACGTAGCGAAATGCGATACTTGGTGTGAATTGCAGA CGCATCGATGAAGAACGTAGCGAAATGCGATACTTGGTGTGAATTGCAGA CGCATCGATGAAGAACGTAGCGAAATGCGATACTTGGTGTGAATTGCAGA CGCATCGATGAAGAACGTAGCGAAATGCGATACTTGGTGTGAATTGCAGA CGCATCGATGAAGAACGTAGCGAAATGCGATACTTGGTGTGAATTGCAGA CGCATCGATGAAGAACGTAGCGAAATGCGATACTTGGTGTGAATTGCAGA CGCATCGATGAAGAACGTAGCGAAATGCGATACTTGGTGTGAATTGCAGA CGCATCGATGAAGAACGTAGCGAAATGCGATACTTGGTGTGAATTGCAGA CGCATCGATGAAGAACGTAGCGAAATGCGATACTTGGTGTGAATTGCAGA CGCATCGATGAAGAACGTAGCGAAATGCGATACTTGGTGTGAATTGCAGA -TATGCGATACTTGGTGTGAATTGCAGA CGCATCGATGAAGAACGTAGCGAAATGCGATACTTGGTGTGAATTGCAGG Dicliptera_chinensis Hypoestes_aristata Peristrophe_bivalvis Dicliptera_bupleuroides Rhinacanthus_nasutus Cyclacanthus_poilanei Clinacanthus_nutans Isoglossa_clemensorum Isoglossa_inermis Pachystachys_lutea Ecbolium_ligustrinum Pseuderanthemum_poilanei Pseuderanthemum_polyanthum Graptophyllum_pictum Pseuderanthemum_eberhardtii Codonacanthus_pauciflorus Ptyssiglottis_pubisepala Rungia_pierrei Rungia_sarmentosa Rungia_evrardii Rungia_pectinata Rungia_eberhardtii Rungia_clauda Justicia_fragilis Justicia_gendarussa Justicia_ventricosa Justicia_myuros Justicia_poilanei Justicia_glomerulata Asystasia_gangetica Asystasia_nemorum Thunbergia_coccinea ATCCCGTGAACCATCGAGTTTT-TGAACGCAAGTTGCGCCCGAAGCCTTC ATCCCGTGAACCATCGAGTTTT-TGAACGCAAGTTGCGCCCGAAGCCTTC ATCCCGTGAACCATCGAGTTTT-TGAACGCAAGTTGCGCCCGAAGCCTTC ATCCCGTGAACCATCGAGTTTT-TGAACGCAAGTTGCGCCCGAAGCCTTC ATCCCGTGAACCATCGAGTCTT-TGAACGCAAGTTGCGCCCGAAGCCTTC ATCCCGTGAACCATCGAGTCTT-TGAACGCAAGTTGCGCCCAAAGCCCTC ATCCCGTGAACCATCGAGTCTT-TGAACGCAAGTTGCGCCCGAAGCCCTC ATCCCGTGAACCATCGAGTCTT-TGAACGCAAGTTGCGCCCGAAGCCCTC ATCCCGTGAACCATCGAGTCTT-TGAACGCAAGTTGCGCCCGAAGCCCTC ATCCCGTGAACCATCGAGTCTTGTGAACGCGAGTTGCGCCCGAAGCCTTC NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN ATCCCGTGAACCATCGAGTCTT-TGAACGCAAGTTGCGCCCGAAGCCCTC ATCCCGTGAACCATCGAGTCTT-TGAACGCAAGTTGCGCCCGAAGCCCTC ATCCCGTGAACCATCGAGTCTT-TGAACGCAAGTTGCGCCCAAAGCCCTC ATCCCGTGAACCATCGAGTCTT-TGAACGCAAGTTGCGCCCGAAGCCCTC ATCCCGTGAACCATCGAGTCTT-TGAACGCAAGTTGCGCCCAAAGCCCTC ATCCCGTGAACCATCGAGTCTT-TGAACGCAAGTTGCGCCCGAAGCCTTC ATCCCGTGAACCATCGAGTCTT-TGAACGCAAGTTGCGCCCGAAGCCTTC ATCCCGTGAACCATCGAGTCTT-TGAACGCAAGTTGCGCCCGAAGCCTTC ATCCCGTGAACCATCGAGTCTT-TGAACGCAAGTTGCGCCCGAAGCCCTC ATCCCGTGAACCATCGAGTCTT-TGAACGCAAGTTGCGCCCGAAGCCCTC ATCCCGTGAACCATCGAGTCTT-TGAACGCAAGTTGCGCCCGAAGCCTTC ATCCCGTGAACCATCGAGTCTT-TGAACGCAAGTTGCGCCCGAAGCCTTC ATCCCGTGAACCATCGAGTCTT-TGAACGCAAGTTGCGCCCGAAGCCTTC ATCCCGTGAACCATCGAGTCTT-TGAACGCAAGTTGCGCCCGAAGCCTTC ATCCCGTGAACCATCGAGTCTT-TGAACGCAAGTTGCGCCCGAAGCCTTC ATCCCGTGAACCATCGAGTCTT-TGAACGCAAGTTGCGCCCGAAGCCTTC ATCCCGTGAACCATCGAGTCTT-TGAACGCAAGTTGCGCCCGAAGCCTTC ATCCCGTGAACCATCGAGTCTT-TGAACGCAAGTTGCGCCCGAAGCTTTT ATCCCGTGAACCATCGAGTCTT-TGAACGCAAGTTGCGCCCGAAGCCTTC ATCCCGTGAACCATCGAGTCTT-NGAACGCAAGTTGCGCCCGAAGCCTTC ATCCCGTGAACCATCGAGTCTT-TGAACGCAAGTTGCGCCCGAAGCCATT viii Dicliptera_chinensis Hypoestes_aristata Peristrophe_bivalvis Dicliptera_bupleuroides Rhinacanthus_nasutus Cyclacanthus_poilanei Clinacanthus_nutans Isoglossa_clemensorum Isoglossa_inermis Pachystachys_lutea Ecbolium_ligustrinum Pseuderanthemum_poilanei Pseuderanthemum_polyanthum Graptophyllum_pictum Pseuderanthemum_eberhardtii Codonacanthus_pauciflorus Ptyssiglottis_pubisepala Rungia_pierrei Rungia_sarmentosa Rungia_evrardii Rungia_pectinata Rungia_eberhardtii Rungia_clauda Justicia_fragilis Justicia_gendarussa Justicia_ventricosa Justicia_myuros Justicia_poilanei Justicia_glomerulata Asystasia_gangetica Asystasia_nemorum Thunbergia_coccinea GGGCCGAGGGCACGCCTGCCTGGGCGTCACGCATCGCGTCGCCC -C GGGCCGAGGGCACGCCTGCCTGGGCGTCACGCATCGCGTCGCCC -C GGGCCGAGGGCACGCCTGCCTGGGCGTCACGCATCGCGTCGCCC -C GGGCCGAGGGCACGCCTGCCTGGGCGTCACGCATCGCGTCGCCC -C GGGCCGAGGGCACGCCTGCCTGGGCGTCACGCATCGCGTCGCCC -C GGGCCGAGGGCACGCCTGCCTGGGCGTCACGCATCGCGTCGCCC CGGGCCGAGGGCACGCCTGCCTGGGCGTCACGCATCGCGTCGCCC CT GGGCCGAGGGCACGCCTGCCTGGGCGTCACGCATCGCGTCGCCC CGGGCCGAGGGCACGCCTGCCTGGGCGTCACGCATCGCGTCGCCC CGGGCCGAGGGCACGCCTGCCTGGGCGTCACGCATAGTGTCGCCC CCCC NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNGCCCCC GGGCCGAGGGCACGCCTGCCTGGGCGTCACGCATCGCGTCGCCC -C GGGCCGAGGGCACGCCTGCCTGGGCGTCACGCATCGCGTCGCCC -C GGGCCGAGGGCACGCCTGCCTGGGCGTCACGCATCGCGTCGCCC -C GGGCCGAGGGCACGCCTGCCTGGGCGTCACGCATCGCGTCGCCC -C GGGCCGAGGGCACGCCTGCCTGGGCGTCACGCATCGCGTCGCCC -C GGGCCGAGGGCACGCCTGCCTGGGCGTCACGCATCGCGTCGCCC -C GGGCCGAGGGCACGCCTGCCTGGGCGTCACGCATCGCGTCGCCC -C GGGCCGAGGGCACGCCTGCCTGGGCGTCACGCATCGCGTCGCCC -C GGGCCGAGGGCACGCCTGCCTGGGCGTCACGCATCGCGTCGCCC -C GGGCCAAGGGCACGCCTGCCTGGGCGTCACGCATCGCGTCGCCC -C GGGCCGAGGGCACGCCTGCCTGGGCGTCACGCATCGCGTCGCCC -C GGGCCGAGGGCACGCCTGCCTGGGCGTCACGCATCGCGTCGCCC -C GGGCCGAGGGCACGCCTGCCTGGGCGTCACGCATCGCGTCGCCC -C GGGCCGAGGGCACGCCTGCCTGGGCGTCACGCATCGCGTCGCCC -C GGGCCGAGGGCACGCCTGCCTGGGCGTCACGCATCGCGTCGCCC -C GGGCCGAGGGCACGCCTGCCTGGGCGTCACGCATCGCGTCGCCC -C GGGCCGAGGGCACGCCTGCCTGGGCGTCACGCATCGCGTCGCCC -C GGGCCGAGGGCACGCCTGCCTGGGCGTCACGCATCGCGTCGCCC -T GGGCCGAGGGCACGCCTGCCTGGGCGTCACGCATCGCGTCGCCC -C GGGCCGAGGGCACGCCTGCCTGGGCGTCACGCATCGCGTCGCCC -C AGGCCGAGGGCACGTCTGCCTGGGCGTCACGCATCGCGTCGCCC -C Dicliptera_chinensis Hypoestes_aristata Peristrophe_bivalvis Dicliptera_bupleuroides Rhinacanthus_nasutus Cyclacanthus_poilanei Clinacanthus_nutans Isoglossa_clemensorum Isoglossa_inermis Pachystachys_lutea Ecbolium_ligustrinum Pseuderanthemum_poilanei Pseuderanthemum_polyanthum Graptophyllum_pictum Pseuderanthemum_eberhardtii Codonacanthus_pauciflorus Ptyssiglottis_pubisepala Rungia_pierrei Rungia_sarmentosa Rungia_evrardii Rungia_pectinata Rungia_eberhardtii Rungia_clauda Justicia_fragilis Justicia_gendarussa Justicia_ventricosa Justicia_myuros Justicia_poilanei Justicia_glomerulata Asystasia_gangetica Asystasia_nemorum Thunbergia_coccinea CCCCCTCC-ACCCCGTCCTCGGTAGGCGGGGC -GGCCGGGGGG-GCGG CTCCT CCCGTCCTCTGTTGGCGGGGC -GGCCGGGG -GCGG CTCCC -CCCCGCCCTC-GTTGGCGGGGC -GGCCGGGGGGGGCGG CCTCC ACCCCGTCCTCAGTAGGCGGGGC -GGCCGGGGG GCGG CCTCCCCCCCGCCCGCTCCCACGCGGAGCGGCCGGGACCGGGGGGGGCGG -CCCTCCCCCGCTCCC GGCGGA-GCGGGC-GAG-TGCGGGGGGCGG CCCCTCCCCCGCTCCC GACGGA-GCGGGC-GGG-TGCGGGGGGCGG -CTCTCCCCCGCTCCC GACGGA-GCGGAC-GGG-TGCGGGGGGCGG -CTCTCCCCCGCTCCC GATGGA-GCGGAC-GGG-TGCGGGGGGCGG CAACCCGCCCGCTCC GGGC-GGGGCGCGGGGGGCGG CCACCCTCCCGCTCCCCGACGACGGG-GCGGGC-GGGGCACGGGGG-CGG CCTCCCCTGC TCCC -ACGGA-GCGGGT-GGGGCGGGGG -CGG CCTCCCCTGC TCCC -ACGGA-GCGGGT-GGGGCGGGGG -CGG CCTCCCCTGC TCCT -RAGGA-GTGGGY-GGGGCGGGGG -CGG CCTCCCCTGC TCCC -ACGGA-GCGGGC-GGGACGGGGG -CGG CCTCCCTCGC TCCC -ACGGA-GCGGGC-GGGGCGGGGG -CGG CCTCCCCTGC TCCC -ACGGA-GCGGGC-GGGGCGGGGG -CGG CTCCCCCC-G-CTCCC -ACGGA-GCGGGC-TCGGCGGGGG -CGG CTCCCCCC-G-CTCCC -ACGGA-GCGGGC-TCGGCGGGGG -CGG CCTCCCCCCG-CTCCA -ACGGA-GCGGGCCTCGGTGGGGGGGGCGG CTTACCCC-G-CTCCC -ACGGA-GCGGGCCTCGGCGAGGGGG-CGG CTCCCCCC-G-CTCCC -AAGGA-GCGAGC-GTGGAGGGGG -CGG CTCCCCCC-G-CTCCC -CAGGA-GCGAGC-TTGGAGGGGG -CGG CTCCCCCC -CTCCC -ACGGA-GCGGCG GGGCCGGGG -CGG CTCCCCCC -CTCCC -ACGGA-GCGGCG GGGCCGGGG -CGG CTCCCCCC -CTCCC -ACGGA-GCGGCG GGGCCGGGG -CGG ATCCCCCTCGCATCCC -ATGGATGCGGGC-GGGACGGGAG -TGG ATCCCACCCC TCCC -ACGGA-GCGGGC-GGGACGGGAG -CGG ATCCCCCTTC TCCC -ACGGA-GTGGGC-GGGACGGGAG -CGG CTCCCCCC -ATCGC -GTGTGGGG GGGCGGGGG -CGG CTCCCCCC -ATCGC -GTGTGGGG GGGCGGGGG -CGG CCTCCCACGCCCGCAC GGCCGG-GCGGCG-GGAGCGGGGG -CGG ix Dicliptera_chinensis Hypoestes_aristata Peristrophe_bivalvis Dicliptera_bupleuroides Rhinacanthus_nasutus Cyclacanthus_poilanei Clinacanthus_nutans Isoglossa_clemensorum Isoglossa_inermis Pachystachys_lutea Ecbolium_ligustrinum Pseuderanthemum_poilanei Pseuderanthemum_polyanthum Graptophyllum_pictum Pseuderanthemum_eberhardtii Codonacanthus_pauciflorus Ptyssiglottis_pubisepala Rungia_pierrei Rungia_sarmentosa Rungia_evrardii Rungia_pectinata Rungia_eberhardtii Rungia_clauda Justicia_fragilis Justicia_gendarussa Justicia_ventricosa Justicia_myuros Justicia_poilanei Justicia_glomerulata Asystasia_gangetica Asystasia_nemorum Thunbergia_coccinea AGACTGGCCTCCCGTGCGCTCCCGTCGCGCGGCCGGCCCAAACGCGGTCC ATTATGGCCTCCCGTGCGCTCCCGTCGTGCGGCCGGCCCAAACGCGGTCC AGAATGGCCTCCCGTGCGCCCCCGTCGCGCGGCCGGCCCAAACGCGGTCC AGACTGGCCTCCCGTGCGCTCTCGTCGCGCGGCCGGCCCAAACGCGGTCT AGACTGGCCTCCCGTGCGCTCCCGTCGCGCGGCCGGCCCAAATGCGATCC AGACTGGCCTCCCGTGCGCTCCCGTCGCGCGGCCGGCCCAAATGCGATCC AGACTGGCCTCCCGTGCGCTCCCGTCGCGCGGCCGGCCCAAATGCGATCC AGACTGGCCTCCCGTGCGCTCCCGTCGCGCGGCCGGCCCAAATGCGATCC AGACTGGCCTCCCGTGCGCTCCCGTCGCGCGGCTGGCCCAAATGCGATCC AGACTGGCCTCCCGTGCGCCTCAGTCGCGCGGCCGGCCCAAATGCGATCC AGACTGGCCTCCCGTGCGCTCACGCCGCGCGGCCGGCCCAAATGCGATCC AGATTGGCCTCCCGTGCTCCCC-GTCGTGCGGCCGGCCCAAATGCGATCC AGATTGGCCTCCCGTGCGCCCC-GTCGTGCGGCCGGCCCAAATGCGATCC AGATTGGCCTCCCGTGCGCCCT-GTTGTGCGGCCGGCCCAAATGCGATCC AGATTGGCCTCCCGTGCGCCCC-GTCGTGCGGCCGGCCCAAATGCGATCC AGACTGGCCTCCCGTGTGCCCC-GTCGCGCGGCCGGCCCAAATGCGATCC AGACTGGCCTCCCGTGCGCCCCCGTCGCGCGGCCGGCCCAAATGCGATCC AGAATGGCCTCCCGTGCGCTCCCGTCGCGCGGCCGGCCCAAATGCGATCC AGAATGGCCCCCCGTGCGCTCCCGTCGCGCGGCCGGCCCAAATGCGATCC AGACTGGCCTCCCGTGCGCTCCCGTCGCGCGGCCGGCCCAAATGCGATCC AGACTGGCCTCCCGTGCGCTCCCGTCGCGCGGCCGGCCCAAATGCGATCC ATACTGGCCTCCCGTGCGCTCCCGTCGTGCGGCCGGCCCAAATGCGATCC AAACTGGCCTCCCGTGCGCTCCCGTCGTGCGGCCGGCCCAAATGCGATCC AGACTGGCCTCCCGTGCGCTACCATCGCGCGGCCGGCCTAAATGCGATCC AGACTGGCCTCCCGTGCGCTACCGTCGCGCGGCCGGCCTAAATGCGATCC AGACTGGCCTCCCGTGCGCTACCATCGCGCGGCCGGCCTAAATGCGATCC AGACTGGCCTCCCGTGCGCCAACGTCGCGCGGCCGGCCCAAACGCGATCC AGACTGGCCTCCCGTGCGTCGATATCGCGCGGCTGGCCCAAACGCGATCC AGACTGGCCTCCCGTGCGCTAATGTCGTGCGGCTGGCCCAAACACGATCC AGACTGGCCTCCCGTGCGCCCCCGCCGCGCGGCCGGCCRAAATGCGATCC AGATTGGCCTCCCGTGCGCCCCCGCCGCGCGGCCGGCCCAAATGCGATCC AGATTGGCCTCCCGTGCGCAYGAG-CGCGCGGACGGCCCAAATCGGATCC Dicliptera_chinensis Hypoestes_aristata Peristrophe_bivalvis Dicliptera_bupleuroides Rhinacanthus_nasutus Cyclacanthus_poilanei Clinacanthus_nutans Isoglossa_clemensorum Isoglossa_inermis Pachystachys_lutea Ecbolium_ligustrinum Pseuderanthemum_poilanei Pseuderanthemum_polyanthum Graptophyllum_pictum Pseuderanthemum_eberhardtii Codonacanthus_pauciflorus Ptyssiglottis_pubisepala Rungia_pierrei Rungia_sarmentosa Rungia_evrardii Rungia_pectinata Rungia_eberhardtii Rungia_clauda Justicia_fragilis Justicia_gendarussa Justicia_ventricosa Justicia_myuros Justicia_poilanei Justicia_glomerulata Asystasia_gangetica Asystasia_nemorum Thunbergia_coccinea CCCGGCGGCGCCA-GTCGCGACCAGTGGTGGTTGAAATCTACTCAGCACA CCCGGCGGCGCCA-GTCGCGATCAGTGGTGGTTGAA -TCCTCAGCTCA CCCGGCGGCGCCA-GTCGCGACCAGTGGTGGTTGAA -CCCTCAGCTCA CCCGTCGGCTCCA-GTCGCGACCAGTGGTGGTTGAA -ACCTCAGCTCA CCCGGCGGCGCCA-GTCGCGACCAGTGGTGGTTGAG CTCTTCAACTCG CCCGGCGGCGCCA-GTCGCGACCAGTGGTGGTTGAAT -CCTCAACTCG CCCGGCGGCGCCA-GTCGCGACCAGTGGTGGTTGAAT -CCTCAACTCG CCCGGCGGCGCCA-GTCGCGACCAGTGGTGGTTGAAT -CCTCAACTCG CCCGGCGGCGCCA-GTCGCGACCAGTGGTGGTTGAAT -CCTCAACTCG CCCGGCGGCGCCA-GTCGCGACCAGTGGTGGTTGAATTATCCTCAACTCG CCCGGCGGCGCCA-GTCGCGACCAGTGGTGGTTGAAT -CCTCAACTCG CCCGGCGACGTGC-GTCGCGACCAGTGGTGGTTGAAT -CATCAACTCG CCCGGCGACGTGT-GTCGCGACCAGTGGTGGTTGAAT -CATCAACTCG CCCGGCGACGTGC-GTCGCGACCAGTGGTGGTTGAAT -CATCAACTCG CCCGGCGACGTGC-GTCGCGACCAGTGGTGGTTGAAT -CATCAACTCG CCCGGCGACGTGC-GTCGCGACCAGTGGTGGTTGAAT -TGTCAACTCG CCCGGCGGCGCCA-GTCGCGACCAGTGGTGGTTGAAT -C-TCAACTCG CCCGGCGGCGCCA-GTCGCGACCAGTGGTGGTTGAAT -CCTCAACTCG CCCGGCGGCGCCA-GTCGCGACCAGTGGTGGTTGAAT -CCTCAACTCG CCCGGCGGCGCCA-GTCGCGACCAGTGGTGGTTGAAC -CCTCAACTCG CCCGGCGGCGCCA-GTCGCGACCAGTGGTGGTTGAAC -CCTCAACTCG CCCGGCGGCGCCA-GTCGCGACCAGTGGTGGTTGAAT -TCTCAACTCG CCCGGCGGCGCCA-GTCGCGACCAGTGGTGGTTGAAT -TCTCAACTCG CCCGGCGGCGCTT-GTCGCGACCAGTGGTGGTTGAAT -TCTCAACTCG CCCGGCGGCGCTT-GTCGCGACCAGTGGTGGTTGAAT -TCTCAACTCG CCCGGCGGCGCCT-GTCGCGACCAGTGGTGGTTGAAT -CCTCAACTCG CTCGGCGGTGCCA-GTCGCGACCAGTGGTGGTTGAAT -CCTCAACTCG CCCGGCGGCGCCA-GTCGCGACCAGTGGTGGTTGAAT -CCTCAACTCG CTCAATGGCGCTA-GTCGCGACCAGTGGTGGTTGAAT -CCCCAACTCG CCCGGCGGCGCCCCGTCGCGACCAGTGGTGGTTGAGT -CCCCGACTCG CCCGGCGGCGCCCCGTCGCGACCAGTGGTGGTTGAGT -CCCCGACTCG CCCGGCGGCGCAA-GTCACGGCCAGTGGTGGTTGAGG ACATCGACTCT x Dicliptera_chinensis Hypoestes_aristata Peristrophe_bivalvis Dicliptera_bupleuroides Rhinacanthus_nasutus Cyclacanthus_poilanei Clinacanthus_nutans Isoglossa_clemensorum Isoglossa_inermis Pachystachys_lutea Ecbolium_ligustrinum Pseuderanthemum_poilanei Pseuderanthemum_polyanthum Graptophyllum_pictum Pseuderanthemum_eberhardtii Codonacanthus_pauciflorus Ptyssiglottis_pubisepala Rungia_pierrei Rungia_sarmentosa Rungia_evrardii Rungia_pectinata Rungia_eberhardtii Rungia_clauda Justicia_fragilis Justicia_gendarussa Justicia_ventricosa Justicia_myuros Justicia_poilanei Justicia_glomerulata Asystasia_gangetica Asystasia_nemorum Thunbergia_coccinea -TCTGA-CGGTCGCGTGCATCGGCGTCGTCCGCTC GGGAGCCATCG -GCTGA-CAGTCGCGTGCTTCGGCGTCGTCCGGTC GGG-GCAAGAG -TCTGA-CGGTCGCGTGCATCGGCGTCGTCCGGCC GGGCATCGTCG -TCTGA-CGGTCGTGTGCGTCGGCGTCGTTCGGTC GGGAACCATCG CGTGCTGA-CAGTCGCGGGCTGCGGCGTCGTCCGGTC GGGCATCACCG CGTGCTGT-CAGTCGCGCGGTAGGGCGTCGTCCGGCC GGGCATCACGA CGTGCTGT-CAGTCGCGCGGTAGGGCGTCGTCCGGCC GGGCATCACGA CGTGCTGT-CAGTCGCGCGGTAGGGCGTCGTCCGGCC GGGCATCACGA CGTGCTGT-CAGTCGTGCGGTAGGGCGTCGTCCGGCC GGGCATCACGA CGTGCTGT-CAGTCGTGCGGAGCGGCGTCGTCCGTCCAGGG-CATCACCG CGTGCTGT-CAGTCGTGCGGAGCGGCGTCGTCCGGCCAGGGGCATCACGA CGTGCTGT-CAGTCGTGCTCCACGGCGTCGTCCGGCC GG-CATCACGCGTGCTGT-CAGTCGTGCTCCACGGCGTCGTCCGGTC GG-CATCACGCGTGCTGT-CAGTCGTGCTCCACGGCGTCGTCCGGTC GG-CATCACGCGTGCTGT-CAGTCGTGCTCCACGGCGTCGTCCGGTC GG-CATCATGCGTGCTGT-CAGTCGTGCTCCACGGCGTCGTCCGGTC GG-CATCATGCGTGCTGT-CAGTCGTGCGGAACGGCGTCGTCCGGCT GGGCATCACACGTGCTGA-CAGTCGTGCCGAACGGCGTCGTCCGGAC GGGCATCACCT CGTGCTGA-CAGTCGTGCCGAACGGCGTCGTCCGGAC GGGCATCACCT CGTGCTGA-CAGTCGTGCCGCACGGCGTCGTCCGGTA GGGCATCACTT CGTGCTGA-TAGTCGTGCCGCACGGCGTCGTCCGGTC GGGCATCACCT CGTGCTGA-CAGTCGTGCCGAACCGCGTCGTCCGGCC GGGCTTCACCT CGTGCTGA-CTGTCGTGCCGAACCGCGTCGTCCGGCC GGGCATCACCT CGTGCTGA-CAGTCGCGCCGAACGGCGTCGTCCGGAC GAGCATCACCT CGTGCTGA-CAGTCGCGCCGAACGGCGTCGTCCGGAC GGGCATCACCT CGTGCTGA-CAGTCGCGCCGAACGGCGTCGTCCGGAC GAGCATCACCT CGTGCTGA-CAGTCGCGCCGAACGGCGCCGTCCGGAC GGGCATCACCC CGTGCTGA-CAGTCGCGCCAAACGGCRCCGTCCGGAC GGGCATCACCC CGTGCTGA-CAGTCGTGCTGAATGGCGCCGTTTAGAT GGGCATCACTC CGCGCTGTGTGGTTGCGCTCCGCGGCGTCGTCCGGAC GGGCATCGGAA CGCGCTGTGTGGTCGCGCTCCGCGGCGTCGTCCGGAC GGGCATCGGAA CGTGCTGA-CAGCCGTGCACAACCGCGTCGCCTTTC -GGGAACCGGGA Dicliptera_chinensis Hypoestes_aristata Peristrophe_bivalvis Dicliptera_bupleuroides Rhinacanthus_nasutus Cyclacanthus_poilanei Clinacanthus_nutans Isoglossa_clemensorum Isoglossa_inermis Pachystachys_lutea Ecbolium_ligustrinum Pseuderanthemum_poilanei Pseuderanthemum_polyanthum Graptophyllum_pictum Pseuderanthemum_eberhardtii Codonacanthus_pauciflorus Ptyssiglottis_pubisepala Rungia_pierrei Rungia_sarmentosa Rungia_evrardii Rungia_pectinata Rungia_eberhardtii Rungia_clauda Justicia_fragilis Justicia_gendarussa Justicia_ventricosa Justicia_myuros Justicia_poilanei Justicia_glomerulata Asystasia_gangetica Asystasia_nemorum Thunbergia_coccinea TC GACCCATCCGGCGCCATTTAAGAGGGTGCTTTCGACCGCGACCCCA AG AGTAGACCCAGC -CT GACCCATCCGGCGCCTC -GGCGCCTTCGACCGCGACCCCA TC GACCCATCCGGTGCCATTAGAAATGGCGCTTTCGACCGCGACCCCA -C GACCCAGCCAGCGCCTC -GGCGCTTCCGACCGC GC GACCCAATCGGCGCCTC -GGCGCTTCCGACCGCGACCCCA GC GACCCAATCGGCGCCTC -GGCGCTTTCGACCGC GC GACCCAATCGGCGCCTC -GGCGCTTCCGACCGCGACCCCA GC GACCCAATCGGCGCCTC -GGCGCTTCCGACCGCGACCCCA GC GACCCAATCGGCGCCTC -GGCGCTTCCGACCGC GTTTGACCCGATCGGCGCCTC -GGCGCTTCCGACCGCGACCCCA AA-AGACCCAACCAGCGCCTC -GGCGCTTCCGACCGCGACCCCA AA-TGACCCAACCAGCGCCTC -GGCGCTTCCGACCGCGACCCCA A CGACCCAACCAGCGCCTC -GGCGCTTCCGACCGCGACCCCA AA-CGACCCAACCAGCGCCTC -GGCGCTTCCGACCGCGACCCCA AA-CGACCCAACCAGCGCCTC -TGCGCTTCCGACCGCGACCCCA AA-CGACCCAATCAGCGCCTT -GGCGCTTTCGACCGCGACCCCA TC-CGACCCAACCAGCGCCCC -GGCGCTTTCGACCGCGACCCCA TC-CGACCCAACCAGTGCCCC -GGCGCTTTCGACCGCGACCCCA TA-CGACCCAACCAGCGCCCCC GGCGCTTCCGACCGCGACCCCA TA-CGACCCAACCAGCGCCCCC GGCGCTTCCGACCGCGACCCCA GC-CGACCCAACTAGCGCCGA -TGCGCTTTCGACCGCGACCCCA GC-CGACCCAACCGGCGCAGA -TGCGCTTCCGACCGCGACCCCA GT-CGACCCAACCAGCGCCAC -GGCGCTTCCGACCGCGACCCCA GT-CGACCCAACCAGCGCCAC -GGCGCTTCCGACCGCGACCCCA GT-CGACCCAACCAGCGCCGC -GGCGCTTCCGACCGCGACCCCA GT-CGACCCAACCAGCGCC-C -GACGCTTACGACCGGGACCCCA GT-CGACCCAACCAGCGCCTC -GGCGCTTACGACCGCGACCCCA AT-CGACCCAGCTAGCGCTTT -GGCGCTTACGACCGCGACCCCA AC GACCCAGCCAGCGCCTCG CGCGCTTCCGACCGC AC GACCCAGCCAGCGCCTCG CGCGCTTCCGACCGCGACCCCA AC GACCCA-TCGGCGCACT -CGCGCCTTCGACGGCGACCCCA xi Dicliptera_chinensis Hypoestes_aristata Peristrophe_bivalvis Dicliptera_bupleuroides Rhinacanthus_nasutus Cyclacanthus_poilanei Clinacanthus_nutans Isoglossa_clemensorum Isoglossa_inermis Pachystachys_lutea Ecbolium_ligustrinum Pseuderanthemum_poilanei Pseuderanthemum_polyanthum Graptophyllum_pictum Pseuderanthemum_eberhardtii Codonacanthus_pauciflorus Ptyssiglottis_pubisepala Rungia_pierrei Rungia_sarmentosa Rungia_evrardii Rungia_pectinata Rungia_eberhardtii Rungia_clauda Justicia_fragilis Justicia_gendarussa Justicia_ventricosa Justicia_myuros Justicia_poilanei Justicia_glomerulata Asystasia_gangetica Asystasia_nemorum Thunbergia_coccinea GGTCAGGCGGGATTACCCGCTGAGTTTAAGCAT -GGTCAGGCGGGATTACCCGCTGAGTTTAAGCATATCAATAA GGTCAGGCGGGATTACCCGCTGAGTTTAAGCATATCATAA GGTCAGGCGGGATTACCCGCTGAGTTTAAGCTTATCAATAAGCGGGAGGA -GGTCAGGCGGGATTACCCGCTGAGTTTAAGCATATCATA GGTCAGGCGGGATTACCCGCTGAGTTTAAGCATATCATA -GGTCAGGCGGGATTACCC -GGTCAGGCGGGATTACCCGCTGAGTTTAAGCATATCA GGTCAGGCGGGATTACCCGCTGAGTTTAAGCATATCA GGTCAGGCGGGATTACCCGCTGAGTTTAAGCATATCAA -GGTCAGGCGGGATTACCCGCTGAGTTTAAGCATATCATAA -GGTCAGGCGGGATTACCCGCTGAGTTTAAGCATAT GGTCAGGCGGGATTACCCG GGTCAGGCGGGATTACCCGCTGAGTTTAAGCATATCA GGTCAGGCGGGATTACCCGCTGAGTTTAAGCATATC -GGTCAGGCGGGATTACCCGCTGAGTTTAAGCATATCA GGTCAGGCGGGATTACCCGCTGAGTTTAAGCATATCA GGTCAGGCGGGATCACCCGCTGAGTTTAAGCATATC -GGTCAGGCGGGATCACCCGCTGAGTTTAAGCATATCA GGTCAGGCGGGATTACCCGCTGAGTTTAAGCATATC -GGTCAGGCGGGATTACCCGCTGAGTTTAAGCATATC -GGTCAGGCGGGATTACCCGCTGAGTTTAAGCATATCA GGTCAGGCGGGATTACCCGCTGAGTTTAAGCATATCA GGTCAGGCGGGATTACCCGCTGAGTTTAAGCATATCA GGTCAGGCGGGATTACCCGCTGAGTTTAAGCATATC GGTCAGGCGGGATTACCCGCTGAGTTTAAGCATATCAATAAGCGGAGGAA GGTCAGGCGGGATCACCCGCTGAGTTTAAGCATATCAA Dicliptera_chinensis Hypoestes_aristata Peristrophe_bivalvis Dicliptera_bupleuroides Rhinacanthus_nasutus Cyclacanthus_poilanei Clinacanthus_nutans Isoglossa_clemensorum Isoglossa_inermis Pachystachys_lutea Ecbolium_ligustrinum Pseuderanthemum_poilanei Pseuderanthemum_polyanthum Graptophyllum_pictum Pseuderanthemum_eberhardtii Codonacanthus_pauciflorus Ptyssiglottis_pubisepala Rungia_pierrei Rungia_sarmentosa Rungia_evrardii Rungia_pectinata Rungia_eberhardtii Rungia_clauda Justicia_fragilis Justicia_gendarussa Justicia_ventricosa Justicia_myuros Justicia_poilanei Justicia_glomerulata Asystasia_gangetica Asystasia_nemorum Thunbergia_coccinea AAGAAACTTACGAGGATTCCCCTAGTAACGGCGAGCGAACCGGGAATAGC xii Dicliptera_chinensis Hypoestes_aristata Peristrophe_bivalvis Dicliptera_bupleuroides Rhinacanthus_nasutus Cyclacanthus_poilanei Clinacanthus_nutans Isoglossa_clemensorum Isoglossa_inermis Pachystachys_lutea Ecbolium_ligustrinum Pseuderanthemum_poilanei Pseuderanthemum_polyanthum Graptophyllum_pictum Pseuderanthemum_eberhardtii Codonacanthus_pauciflorus Ptyssiglottis_pubisepala Rungia_pierrei Rungia_sarmentosa Rungia_evrardii Rungia_pectinata Rungia_eberhardtii Rungia_clauda Justicia_fragilis Justicia_gendarussa Justicia_ventricosa Justicia_myuros Justicia_poilanei Justicia_glomerulata Asystasia_gangetica Asystasia_nemorum Thunbergia_coccinea ; end; CCAACTTGAGAATCGGGCGGCCGCGCCGTCCGAATTGTAGTCTGGA [...]... ng), H N i (Ba V ), Hũa Bỡnh (Mai Chõu), H i Phũng (Cỏt B), Thỏi Bỡnh (Qu nh Ph ), Ninh Bỡnh (Nho Quan: Cỳc Ph ng), Thanh Húa (Nh Thanh, Th Xuõn), Ngh An (Con Cuụng: Pự Mỏt), H T nh (H Tr ch), Qu ng Tr (B c H ng ng S n), Qu ng Bỡnh (B ng Húa), Th a Thiờn-Hu (Phỳ L c: B ch M ), (B N), Qu ng Nam (Nam Giang), Qu ng Ngói (Ba T ), Bỡnh N ng nh (Quy Nh n), Kon Tum (Kon Plụng, Kon R y, Sa Th y), Gia Lai (KBang,... t (Subtrib Justiciinae Nees) thu c h ễ rụ (Fam Acanthaceae Juss. ) l c p thi t, ỏp ng yờu c u c n thi t hi n t i c a t n Vi t Nam c K t qu ti l c khoa h c v phõn lo i h ễ rụ núi chung v phõn tụng Xuõn ti t núi riờng Vi t Nam, gúp ph n biờn so n b sỏch Th c v t chớ Vi t Nam v taxon ny 2 M c ớch c a ti lu n ỏn Hon thnh vi c phõn lo i phõn tụng Xuõn ti t (Justiciinae Nees) thu c h ễ rụ (Acanthaceae Juss. ). .. t Nam, cỏc vựng sinh thỏi khỏc nhau Tỏc gi ó ti n hnh 25 t i u tra thu c 54/63 t nh trong c n c: Lai Chõu, i n Biờn ( i n Biờn, M ng Nh ), Lo Cai (Sa Pa, V n Bn), S n La (Võn H , M c Chõu), H Giang (V Xuyờn, Qu n B , ng V n), Cao B ng 18 (Nguyờn Bỡnh, Trựng Khỏnh), B c K n (Ba B ), Thỏi Nguyờn (Vừ Nhai), L ng S n (Cao L c), Qu ng Ninh (H Long), Phỳ Th , V nh Phỳc (Tx Phỳc Yờn), B c Giang (S n ng),... nghiờn c u, cỏc h th ng phõn lo i h ễ rụ (Acanthaceae) v phõn tụng Xuõn ti t (Justiciinae) 1.2.1 Trờn th gi i T th k 18, Linnaeus (175 3) [104] ng i c coi l ụng t c a ngnh phõn lo i th c v t, ó mụ t v t tờn cho 6 chi v 30 loi m sau ny chỳng c x p vo h ễ rụ (Eranthemum (1 loi), Justicia (11 loi), Acanthus (4 loi), Barleria (5 loi), Ruellia (8 loi), Dianthera (1 loi) Cỏc chi v loi ny c tỏc gi x p vo phõn... Kh ), k L k (Buụn ụn, Krụng Bụng, L k), R L p, Tx Gia Ngh a), Lõm ng (Di Linh, k Nụng ( c Tr ng, Lõm H, Tp k L t), Phỳ Yờn (S n Hũa), Khỏnh Hũa (Diờn Khỏnh, Khỏnh V nh, Tp Cam Ranh, Tp Nha Trang, Tx Ninh Hũa), Ninh Thu n (B c i, Ninh H i, Ninh Ph Thu n (B c Bỡnh, Hm Thu n Nam, Tỏnh Linh), Bỡnh Ph (Tõn Biờn: VQG Lũ Gũ-Xa Mỏt), Tu (Bỡnh Chõu-Ph c B u, Cụn c (Bự Gia M p), Tõy Ninh ng Nai (Tõn Phỳ, Nam. .. monogynia) v 4 nh v i 2 di v 2 ng n (Dydinamia) cựng v i nhi u chi v loi c a nhi u h khỏc cựng cú chung c i m trờn nh h B c h (Lamiaceae), h C roi ng a (Verbenaceae), h Nhi (Oleaceae), A L de Jussieu (178 9) [103] l nh th c v t h c u tiờn ó h th ng hoỏ l i cỏc chi thnh cỏc h riờng bi t v t tờn cho nhi u h th c v t, trong ú cú h Acanthaceae ễng ó s p x p 8 chi (Acanthus, Barleria, Ruellia ,) vo h ny... (Scrophulariales) nh ng thu c cỏc phõn l p khỏc nhau: A Takhtajan (197 3) x p h ễ rụ vo phõn l p Cỳc (Asteridae); V H Heywood (199 3) [50] x p vo phõn l p S (Dilleniidae); A Takhtajan (1987, 199 6) [8969], 154[69] thỡ l i x p h ễ rụ vo phõn l p B c h (Lamiidae) T t c th ng nh t v trớ h ễ rụ n m trong l p M c lan (Class Magnoliopsida, Dicotyledones) thu c ngnh M c lan (Magnoliophyta, Angiospermae) 1.2 Tỡnh... Xuõn ti t ( 4) nh 3.26: Hỡnh nh h t m t s loi thu c phõn tụng Xuõn ti t ( 5) nh 3.27: Hỡnh nh h t m t s loi thu c phõn tụng Xuõn ti t ( 6) nh 3.28: Asystasia neesiana (Wall .) Nees nh 3.29: Asystasia gangetica (L .) T Anders nh 3.30: Asystasia gangetica subsp micrantha (Nees) Ensermu nh 3.31: Pseuderanthemum crenulatum (Lindl .) Radlk x nh 3.32: Pseuderanthemum polyanthum (C B Clarke ex Oliver) Merr nh 3.33:... 1 T NG QUAN TI LI U 1.1 V trớ c a h ễ rụ (Acanthaceae Juss. ) v phõn tụng Xuõn ti t (Justiciinae) trong b Hoa mừm chú (Scrophulariales) v l p M c lan (Magnoliopsida) Tr c khi h ễ rụ chi v loi m sau ny c thnh l p, Linnaeus (175 3) [104] ó t tờn cho m t s c x p vo h ễ rụ, trong ú chi Eranthemum, Justicia c x p vo nhúm 2 nh v i 1 vũi nh y (Diandria monogynia); chi Acanthus, Barleria, Ruellia, Dianthera... thnh rừ rng l tụng Xuõn ti t (Justicieae) Tuy nhiờn cỏc h th ng ny cũn nhi u h n ch ó c p c phớa trờn, vỡ v y c n tỡm ki m thờm cỏc h th ng khỏc so sỏnh 2 Quan i m th 2: Chia h ễ rụ (Acanthaceae) thnh cỏc phõn h (Subfamily), r i chia thnh cỏc tụng (Tribus) v phõn tụng (Subtribus) Ng i t n n múng cho cỏch phõn chia ny ph i k n G Lindau (189 5) [52] ó chia h Acanthaceae thnh 4 phõn h c n c vo c i