CẢI TIẾN PHƯƠNG PHÁP CHIẾT XUẤT CURCUMINOIDS tù NGHỆ VÀNG

B GIO DC V O T B Y T TRNG I HC DC: H NI BI V DNG CI TIN PHNG PHP CHIT XUT CURCUMINOIDS T NGH VNG CHUYấN NGNH CễNG NGH DC PHM V BO CH M S 60 73 01 TRNGHDC Hèè-'.:!; Ngi hng dn khoa hc: TS Nguyn Vón Hõn TS Quyờn H NI - 2011 VV- * VV- 7LI C M O N Tri qua nm hc cao hc ti ó hon thnh khúa hoc v lun ỗỷa minh, Toi i nhn c s hng dn, gỡp y gpp nhợờ-tj tnh ea quớ thy ầễ trng hc Dc H NễL Trc ht, tụi xn ỗhõn thnh ỗm_ n n qui th ỗd trng hoỗ P H Ni a tn tjnh dav hao trng Êt thi gian tụi hoc ti trng Tụi xớu gi li bit n sõu sc en Tn s Nguyn Vtt Hõn v Tia s Quyn, thv nh nhiu th gian v tõm tuyt hng dn xụi hon thnh lun vọn tt nghip ny, Cm ora nhng ngi ban, anh ỗhi em p Co hoc 14 ó.gỳp yiCng tụi hon thnh khúa hc, ny M dự tụ ó c gang hn thn un yn bng tat c s nhit: tnh v, nng lc ca mỡnh nhng khụng th trỏnh Mi nhng thiu sty rt mong nhn c nhng úng gp quý bỏ ca quớ thy cõ vố cỏc ban., Xin cớiõự thnh cm ỡ! H NI Dg 30 thDg 12 nm 2011 Hc viờn Bự Y Dng:MC: LC: DANH MC CC BNG DANH MC CC HèNH T VN' Chng TễNG QUAN ~ \_ Vi nột v cy ngh vjng 1.1 M t cõy: LL.2 Phõn b i , , 1,1,3, Th_nhphn 5'/ 5-r, 10', 1.2 S lc v crcmjiioi\ 1.2' L Thnh phn v tớnh cht 0,2, To g dc v- * 2*3*4* XG nl tnh im toe eaduagm hii 23,5, si;s2^ Phng phỏp cht y Tnh ch pur cumnoMi.- r ~ r ;:-p_ 23,6, 23,7, 23,8, T LIU THAM KHO PH L CNH MC CC BNG 23,40, 23,41, DANH MC CC HèNH V 23,63, 23,69, 23,73, 23,64, thỡ biu dỡ-tt hin suõt ỗhiờt t.hfO' thi gian ca 23,70, ụ th biờu in hiu sut ehớờt theo' thi gian cai bon 23,74, th bi din hiu sut chit theo thi gin ca Bt 23, 23, 23, 23,79, 23,80, T VN â Trong nhng nm gn y, nhm cht mu curcuminừ chit xut t ngh vng thu hỳt nhiu s quan tõm ca cỏc nh nghin cu bi_ nhng tỏc ng snh ho quan trng! c ch_ s phỏt, trin ca cỏc 11* tng kh aang rnr-n dch? chng xy limnh, tỏc dng b y gan, tỏc dng chng viờm, phũngv ch ng th5 vm gn, bờnh lzhejmer; [1] 23,81, n n ó c nh nghn c hỡt xt crcminoớd c v ngoi nc, Nhng a s cỏc phng phỏp ch c gii thiu chung chung m khụng nờu rừ cỏc thụng s chit v tinh chộ c th* VI vy vic tham kMo v tip cn qui trỡnh gp nhiu kh khn, Nhu cu s ng curcurninod ngy mt tng cao, ngun nguyờn liờu Dgh li; sn cú nc, nờn vờc phỏt trin qu trỡnh k tht chit xt, tnh ch: crcumnod s lng ln tr nờn cp thit, T nhu cu th, t ố> chung ti thc hiờn ti; 23,82, 23,83, 23,5, vi nhng mc tiờu: 23,84, ô Cỡ tn c phng phỏp x lv ngờni liu thu c bt ngh cúi hm lng ircumod cao - Xõ dng c phng phỏp chit xt v tnh ch iu c 23,85, eurcumớnoỡd t hm lng trờn 95% 23,86, Chng I TNG QUAN 1.1 V nột v c ngh vng 1.1.1 Mụ t cõy 23,87, Ngh (Curcuma nga L,,; ghraca) l mt: loi cy c ci Q?úm n Im, Thõn r thnh c hỡnh tr hc hi dt, bố o ct: ngang cú m vng am sm Lỏ hỡnh tri xoan, thon nhn hi u, hi mt u nhiiidi ti 45cm, rng ti 18cm, Cung l_ b Cm, hoa mc tu gia ỏc, lỏ {n, thnh hỡnh nún tha, lỏ bc hu th khum hỡnh mỏng rng, u trũn mu xanh lc nht* 1'ỏ bc bt th hp hn, m hi tim nht Ttng c phin, cỏnhi hoa ngoi cú mu xanh lc vng nht, chỡa thnh thựy? thự trờn to hn, phin cỏnh hoa cung chi th th hỡ bờn ng v thng, thự d bom thnh mng sõu Q nng 3i ngn? m bng 3: vaa [8] , 23,88, , 1,2 Phõn bo 23,89, Cõy ngh vng mc hoang v c trng ii , Trung Quc, Lo, Campucha, Indonesia v cỏo nc nhl i nc ta ngh vng trng khỏ ph bin Mt s a phng hỡnh thnh cỏc vựng chuyờn canh, nh Xuõn LC; (ng Nai), Chớ Tõn ( Khoỏi Chõu7 Hng Yờn)5 Ngh An [$] 23,90, 143; Thnh phn 23,91, 10 Thnh phn tớnh dtớ; hm ng 3=7% [25], 23,682, Jayaraj Ravindran et ọ\ (201t)), "BisUemethylcurcumin ?and i$truÊturọlly related hispolon analogues of curcumin exhibit enhanced prooxidant, anti-proliferative and anti mfiammatory activities m vitro", Biochemical: Pharmacology, pp., 1658-1666 23,683, iK.iNirmai JBọbu, IK Sivaramai), JF\N _Rav_indran '(2007), '"Postharv'est technology and processing of tumeric" Tumeric: The Germs Curcuma, pp, 198-199 23,684, iKhopde "Venkatesan ;(.1999)i '?1Free radical scavenging ability and antioxidant efficiency of curcumin and its substituted analogue", Biophys Qhem (80)3 pp 8S-9L 27 Majeedetal et al ([999), 1,gi;Gprotecxant composition, method of us$ Êndi 23,685, 28 extraction process ofeurcuminoids", Patent US586141S Q Ran and X Zhon (1988), "New methods: for isolation of curcumin"* Chem, Abstrr> 109* No 148152r 29 S, Revanthy el aJ (2011), 'Isolation, purification and identification of curcuminoids ftoiri turmeric {Curcuma longa L.) by column chromatography'5 Journalof ExperimentalSciences 201,19 pp 2.1-2:5 30 Salto Sui et al (1993), "Inihibition of the HIV-1 and HIV-2 proteases by curcumin and curcumin boron complexe-sr, Bioorganic and Medicinal Chemistry-, ( J), pp, 415-422 31 iSastri B.S (1970), Curcumin content of tumeric, Res Ind., 14(4), pp.- 258260, 32 ĂSimon A (1998), 'Inhibitory effect of curcuminoids on mcf-7 cell pfoliferationi and simcture-activity relationship11* Cancev let, pp LULL^ 33 ĂSneliasikta Swamakar and iSumit Paul (2009), -'Curcumin arrests endometriosis by down regulation of matrix mettal Loproteinase^9 activity'*, Indian Journal, aflZiochemistry and Biophysics; Vol46? pp 59- 65, 34 Surh Chun iet Ăal (2001), "Molecular mechanisms underlying chemopreventive activities of antMnfLammatory phytochemicals: Downregulation off' COX-2 and inos through suppression of NF-kb activation'' Mutation Research, pp 480-481 35 The United States pharmacopoeia 32 {20QSX 23,686, V, & Govindarjan (1980)% 'Tyiroerio - chemistry, technology, and quality", CSC Critical Review in Food Scfmce and Nutrition pp 199- 301 23,687, Vogel J (1 Đ 15), "Curcumin - biological and medical properties"* ô/, Phavm, 2, pp 20-24 23,688, Yongxiang Yu (2006), Comparison of bioactiviti'eSi and composition of curcumin -free tumeric (Curcuma ionga, L.) oils from different soures? ClemsQn University ? USA, 23,689, Zhang L* and X Zhon (1989), Chem, Abs.tr,, Lll, No 23 L032p.PH LC 23,690, Ph ợỹc t: Sc k Dgh ti 23,691, Ph lc 2: sc ky chun curcuminqd hon hp li ca Vtt kim nghim thuc TW, 23,692, Ph lc 3: sc ký < bt nghờ A (khtg lai tinh bot), 23,693, Ph lc 4: sc- ký bt nghờ- B (ó; loi tỡnh bt) 23,694, Ph lc 5ằ se ký e õn phm ỗureummokl thụ 23,695, Ph c 6: sc k sn phm Curcuminoid tnh khit 23,696, Ph lc 1\ Sc ký dung dich, chula Curcumin 38G160' ca Vin kim nghm thuc TW 23,697, Ph lue 8: sc k chun crcumnod hn hp ca Yim km nghim thuục TW, 23,698, Phu lc 9; sc ký dng dh th r, 23,699, Ph c 10; sc ký ung dich th Ph lc 1; sc ký nghờ ti Ph lc 1; sc ký nghờ ti 23,700, 23,23, , 23,701, chun curcuminoid hn hp 11 23,702, Ph tc 2: sc ký ca Vin kim nghim thuc TW 23,24, 23,25, 9ấ I ô 23,28, Phu fue 3: Sac ky bút nghộ A (khong loaớ tinh bol) 23,26, niAU , 7, 23,703, 23,703, 23,704, 23,706, L * _ _ / ^ r _ i _ _ , _ , 23,30, Phy ớyc 4: Sỏc ky bol nghe B (da loai tinh bot) 23,31, 23,29, 23,707, O Ph lue 5; Saỗ: phõrri thr ký e sn ỗurcuiTĩnoid 23,32, 23,708, 23,705, 23,33, 23,709, Ph lc 6i: Sc ký ụ sn phõm Curcumnod tnh khiờt 23,34, 23,711, 23,710, Ph tC; 7: sc ký dung ỗhuõn ch ctụ Curcumm 580160 ca kim Vin nghiờm thuụỗ TW 23,712, 23,714, 23,716 , A.cq jằed 23,722, D:\Quy\C'mumifti11-1'1XGhuớift feil mỡn 2.'ỡ IM (jy 23,717 , Sam ple Har ne 23,718 , Sam 23,723, Admifi ple 23,732, 23,734, 23,735, 23,35, Cu 23,736, Ph lc 8; Đc k chun curcmno hn hp ca Vin kim nghim thuc TW 23,737, ==== Shimadzu LCsoitỡon Analysis Report ==== 23,739, D:\ỹuyVầĩrcu m Jh\ ỡ-1CM\Chuan hfr.2i1.fcd: Admin : Curcumin : D& tai : i 23,740, : 20 L 23,741, : ầnuarỗ hh 4.ợcd 23,738, 23,742, 23,743, : &iÊmùflÊợJsin : ỗu mi rm in bdtch.lỗb : Repoit-lei 23,744, : liaặQll 4&&Qe FM : 10; 11 /2011 9:01:53 AM 23,745, 23,746, 23,747, 23,748, 23,749, T lay# 1/di#! Injection Acquired by Sample Njme sarn p l Li volume c>ọtọ fils Ndme Methõd F ils Mame 23,750, OTC-ớớrw i 23,751, Batch Fila Hams Report Fil!e Name CằJ1J Acquired Data PớG.cụ55ụd 23,752, 23,753, 23,754, 23,755, 23,756, 23,757, 23,758, 23,36, 23,759, Ph lue 9: sc ký dung dch th 23,760, ==== Shimadzu Analysts Report ==== LCsouon 23,761, & i'.puy'.G u [ ou m i n'.11 -1 c\T lỡu 1.1.1 23,762, ô 23,771, 23,787, 23,788, < Chromatogram:* 23,789, mU 23,790, 300-C 23,791, 'c 23,792, 23,793, L 23,796, 23,797, 23,794, 23,798, l 23,795, 23,799, 23,800, r23,801, co 23,802, ẫ C I 23,803, C 23,804, E 2003 23,805, 23,806, 23,807, 23,808, 23,809, 23,810, X > 23,811, 0 23,812, 01 T3 J 23,813, 23,814, 23,816, 23,817, > X 23,818, b 23,819, JZ 23,820, 23,821, ) 23,822, ĩ1 23,826, ... phâp chiết vạ tinh chế cụrcụiíiínọitỊ - Phượng pháp chiết; chiết eurcuminoid từ bơt nghệ đặ' đưẹ *ử; lỵ từ thận íẫ nghệ vàng bằĩìg phưỡng phấp ngâm íạnhi với dung; mồi thích hợp, - Phương pháp. .. ầơn Chúng tơi tiến hành so sánh bột ngun liệu chể hai phương pháp, phương pháp khơng qua loại tĩnh bột (phương pháp thơng thường) một: phựợng pháp cỗ ]ọạỉ tinh bột: (phựcmg phảp c-ảỉ tiến) Cảc tiêu... nựỞQ rửa phương phấp cất: kéọ h_ợỉ nước 23,318, Tiến hành 5ỉử lý liệu theọ phưong pháp A phương pháp B trên, mỗỉ phương phảp tiên hảnK ỉừ 1,0 kg nghệ ỉươí (hằm lượn 23,319, gcurcuminoid nghệ tươi