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- SPECIFIC PATHOGEN FREE PROGRAMME - (SPF) – INTERNAL INSTITUTE DOCUMENT – DESIGNED BY THANH VU NATIONAL CENTER FOR MARINE SEED RODUCTION IN CENTRAL VIETNAM RESEARCH INSTITUTE FOR AQUACULTURE NO.3 WHITE LEG SHRIMP HATCHERY PRODUCTION TECHNOLOGY          I/ The pre-spawning process            ! "#  #$ ! I/ The pre-spawning process %!%!  &   '()*+,, ! • Employees of shrimp broodstock are produced in Vietnam have been imported from Hawaii that have free 5 pathogens (MBV, IHHNV, TSV, WSSV, YHV). • When using domescated shrimp, it essenal to obtain adequate background informaon on the origin of the stocks and their past performance. Shrimp broodtock’s tanks I/ The pre-spawning process %!+/"  0  !  " !)   " !/ 1! #  ! I/ The pre-spawning process %!+/"'!.  " !  23 $ !  '!! !.  3 /45! Basic laboratory facilities I/ The pre-spawning process %!-  6 7 ! !  ' . ! 6#8 "! 97 !  !)  :! I/ The pre-spawning process %!;  $ !  6 3 3 !  " ' .!    ! %,%+%+%; <' . ! I/ The pre-spawning process %!;'!. • Preferably, the maturation room should have round tanks that are dark-coloured, smooth- sided, and of approximately 5 m diameter. • The broodstock should be held with flow- through (new and/or recycled) water exchange of a total of 250-300% per day and a continuous, but not too vigorous air supply, • Water depth is generally around 0.5-0.7 m, Maturation tanks shape • The shrimp are stocked at a rate of around 6-8 shrimp per sq. m. bottom surface area with a male to female ration of 1-1.5:1. Thus, a 5 m diameter tank can accommodate 60-80 females and 60-100 males. • Water temperatures are usually controlled to be maintained in the range of 28-29 o C, with a salinity of 30-35 ppt and pH of 8.0-8.2. • The feed preparation area should be adjacent to, but separated from, the maturation room I/ The pre-spawning process %!;'!.   '.!  "'. '/=/+, .!   P.vannamei  >?"! - )$ %>"!  % %!@%!(3!  '"$. "'+@ +A  4. $"! . - SPECIFIC PATHOGEN FREE PROGRAMME - (SPF) – INTERNAL INSTITUTE DOCUMENT – DESIGNED BY THANH VU NATIONAL CENTER FOR MARINE SEED RODUCTION IN CENTRAL VIETNAM RESEARCH