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Cacao có tên khoa học là Theobroma cacao L., một loại cây nhiệt đới được người Maya trồng cách đây hơn hai ngàn năm. Theobroma cacao L. có nguồn gốc từ Trung Mỹ, ngày nay được trồng phổ biến ở Brazil, Ghana, Indonesia, Malaysia và một số nước ở lưu vực sông Amazone (Juan Carlos Motamayor, 1999).
SUMMARY Construction of Theobroma cacao L. identification and analysis of genetic diversity using Microsatellites. ABSTRACT-The objective of this thesis is to study the genetic identification of accessions of Theobroma cacao L. from the cocoa collection of Nong Lam University, based on microsatellite markers. Five pairs of primers were used. The amplification products are separated on an ABI 3100 automated DNA sequencing apparatus. The results of this work will help to identify and analyse genetically diversity of cocoa cultivar of the cocoa collection of Nong Lam University. OBJECTIVE 1- Establish Nong lam university cocoa microsatellite database of 21 clones. 2- Study the genetic diversity of 21 clones. 3- Perform multiplex PCR to reduce the cost. 4- Validate a methodology to compare fingerprinting of cocoa accessions. METHOD - Five primers mTcCIR7, mTcCIR8, mTcCIR11, mTcCIR15 and mTcCIR18 were used to find specific microsatellite sequences of 21 cocoa cultivars. - Software Gene Mapper was used to analyse microsatellite database on sequencer ABI 3100. - Software Genetix was used to analyse genetic diversity of 21 cocoa cultivars. CONCLUSION Five microsatellites are enough to compare the identity of all samples. We could use those markers to establish the methodology to perform genetic comparisons in Vietnam. This cacao fingerprint database would be useful in evaluating labeling consistency and mistakes in Vietnamese clonal gardens, and in validation of the identities of clones that have been transferred between cocoa collections. Our methodology of microsatellite analysis is consistent, accurate and harmony with method of ICGD. Another result is performing successfully multiplex PCR to reduce the cost 3 times. Besides, the genetic diversity among 21 cocoa cultivars were established to support the breeder overcome difficulties in plant salection. . apparatus. The results of this work will help to identify and analyse genetically diversity of cocoa cultivar of the cocoa collection of Nong Lam University.. OBJECTIVE 1- Establish Nong lam university cocoa microsatellite database of 21 clones. 2- Study the genetic diversity of 21 clones. 3- Perform multiplex PCR to