Thiết kế thí nghiệm xử lý số liệu nutritional composition and safety aspects of edible insects phân tích anova của fatty acid

BO CONG THUONG TRUONG DAI HOC CONG THUONG TP.HO CHi MINH KHOA CONG NGHE THUC PHAM BAO CAO THUC HANH HOC PHAN: THIET KE THI NGHIEM XU LY SO LIEU NUTRITIONAL COMPOSITION AND SAFETY AS

BO CONG THUONG TRUONG DAI HOC CONG THUONG TP.HO CHi MINH

KHOA CONG NGHE THUC PHAM

BAO CAO THUC HANH HOC PHAN: THIET KE THI NGHIEM XU LY SO LIEU

NUTRITIONAL COMPOSITION AND SAFETY ASPECTS

OF EDIBLE INSECTS: PHAN TICH ANOVA CUA

BO CONG THUONG TRUONG DAI HOC CONG THUONG TP.HO CHi MINH

KHOA CONG NGHE THUC PHAM

BAO CAO THUC HANH HOC PHAN: THIET KE THI NGHIEM XU LY SO LIEU

NUTRITIONAL COMPOSITION AND SAFETY ASPECTS

OF EDIBLE INSECTS: PHAN TICH ANOVA CUA

Coleoptera (beetles, grubs) 35.72 Z14 (061

'€opris nevinsoniè 176 2897 1329 nd 4402 275 4766 $041 392 084 081 557 0.83 Thailand; w Copris nevinsoni Ws , 029 131 2826 185 3171 nd 368 368 178 10368 4024 1294 €Œ29 048 Thailand;w Cybister imbatus Fabriciusđ 29.20 450 360 3730 750 2850 36.00 134 630 180 400 160 2670 053 Thailand;w Helcopris buceph: 091 4034 1325 049 5498 1.61 4063 4224 213 065 nd 278 1.22 Thailand; w Hoiotrichia sp.°! 071 2192 073 2842 O51 559 6.10 nd 1386 4726 nd 333 6451 04? Thailand;w

Holotrichia sp.đè 33.30 30.00 3240 0.53 Thalland;w

Hydrous cavistanum Bedelđ 2200 580 340 3120 320 31.10 34430 21E0 310 nd 710 2.80 3450 045 Thailand; w

+ rhadsmitus) 204 2874 10.70 O49 4196 484 4792 #3416 3# 067 sa 504 07? Thailand; w

Onttis spp.>) 107 1867 1298 391 3663 232 56589 071 8 052 03 9.75 0.58 Thailand; w Onthophsgus mouhot) 125 2165 1121 308 4318 1.73 4509 060 4742 866 043 0.40 939 078 Thailand;w Omhophsgus 166 2386 1782 1.20 4455 298 4300 4696 754 1438 0 951 080 Thailand;w Oryctes owariensis") 250 020 023 012 305 3760 624 079 4363 4546 419 122 Ê0.87 003 Camer;w Oryctes rhinoeeros (Ia.vae)# 350 2820 210 009 3439 441 4150 4591 1410 150 4.10 19.70 082 Nigeria; w Rhynchophorus phoenicis F (larvae)! 320 3240 310 020 3890 330 4010 4340 1300 350 1.20 17.70 0.68 Ngeria:w nynchophorus phoenicis (| 250 39.00 0.30 210 4080 36.00 3.00 0.80 600 - 26.00 2.00 2.00 045 Angola; w Rhynchophorus phoenicis (larvae) oil!” 35.30 6047 9877 q72 072 381 351 Nigeria; w

Tesseratoma papilloaộ) ndđ 046 4100 A6 nd 227 727 nd 467 46.68 nd 61.35 071 Thailand; w

I1 1966 484 111 2661 B31 3787 003 4321 1919 970 Z889 035 Review Aquatic Coleoptera 34 36.20 470 5ứ 3060 052 Review

Terrestric Coleoptera°è 27.10 25.50 250 43.80 80 0.37 Review Diptera 33.02 4723 1695 052

Musca đomestisa (Iarvae)Ÿè 683 2674 232 3689 2692 2175 4767 1644 058 S Kore;r Musca domestica L (pupae)! 320 27680 220 040 3340 2060 1830 3890 1490 210 17200 083 USA;r Diptera!) 281 2255 441 281 2677 2589 099 E512 1218 223 1441 043 Raview

Hemiptera (true bugs) 43.89 3239 Z89 073

Agonoscelis (Thunberg)m) 02L 1141 177 108 2047 104 4115 085 4304 3521 128 3649 028 Sudan;w Aspongubus viduatus Em 034 3133 347 273 3787 1062 4553 062 E677 490 045 535 061 Sudan;w

is indius5) 3180 650 200 30 3450 4250 900 340 nd 410 1.96 18.46 06% Thailand; w Melmuna opaliiera Welker°l 199 247 5283 nd 5699 0.28 1.20 nd 1077 380 nd 80 127 Thailand; w

Hemiptera! 41.63 19.90 328 4183 6481 206 1851 085 1842 8.80 1.43 wss 225

ants) 2388 48.76 Z118 ô(0.43 Carebara viduaS (female thorax)" 106 2706 482 159 3453 186 5144 $330 1219 12.19 0.53 Kenya; w Carebara viduaS (female abdomen)" 183 28.75 594 356 4008 293 4678 4971 1020 1020 0.67 Kenya; w Table 3 Continued

Cl4O CÍẤCO CIEO â Orher ôSFA CIT ôCIT ôOther MUA C2 C3 C3 C203 C204 C205 Other PUEA SFA/ Origin [X tatty selđs] SA "7 MUFA ne a3 6 nề nề ng PUFA UEA, Carebava vidua S (nnale 138 2596 578 172 3482 331 S126 10.81 10.61 053 Kenya;w Polyrhachis vicina Roger (Wenzhou}*! oso 1850 44O 14 2290 320 6080 310 7310 240 080 010 040 020 400 030 Chinaw POlyrhacP4s vicina Roger oso 1 420 15 21.390 “20 S300 1-20 7240 210 180 on ow ow 3790 G31 Ching w Polyrhachis vicina Roger (Zhejiang! 060 1900 430 16 2550 890 6050 190 7130 170 O60 010 020 020 310 O36 Chinar smaraodinuPl 210 2020 S80 32 2190 430 5210 230 S870 700 070 100 030 S40 047 Thailancy w Qecophytta smaragding F (weaver anti 027 119 2767 009 2922 055 180 2 036 oss S782 nd 0.74 ô68.80 041 Thailand: w Cocophyiia smaragdina E {queen caste? 044 168 3241 006 3459 000 1.96 1.96 nd S82 S773 ng 6355 053 Thailand w ' 155 1549 430 155 2134 446 4577 S022 1069 155% 2623 028 Review lsoptera (teemltea) “37 2.00 36046 672

‘Macrotermes bellicosus 018 485 4672 209 128 43 2442 285 3827 088 Nigeria; w Macrotermes bellicosus oil 257 4245 286 150 4898 210 1584 7st 2424 390 as 33.08 095 Nigeria, w

31239 714đ 020 2935 062 5245 SAD7 7.57 157 085 Nigeria; w Tarmes sp? 013 078 3190 002 3283 019 1.86 205 om 890 SS01 0.00 6525 049 Thailand w Lepidoptera (butterflies, moths) 3104 2336 3976 (058 _Anteraea pernyl (ouoael*` 1939 199 060 2251 477 3037 3874 689 3427 ` Bombyx mori (pupse!"! mm (668 nd 2946 060 2601 2661 590 3802 4392 042 Chingr Bomiyx mori (pupaeJ 010 2420 450 28.80 170 2600 ZI70 730 3830 4360 040 Japan,r Bombyx mori (spent pupae) 28670 700 33.20 36.90 3690 420 25.70 23.90 050 Indla;r Cirina forda Westwood (larvae! 070 1200 1600 2110 3100 O20 1390 O50 1460 BAO 4530 020 <010 <010 <010 020 5380 O46 Nigeria;w torasia beting (larvae) 1S 2190 471 012 1788 180 3420 3600 ô602 (19.60 oso 2612 G61 Nigeria; w Imbrasia epimethes eee 060 7320 2210 020 4610 060 840 S00 700 3510 040 4250 090 Congorw Imorasia ert 100 2200 040 2950 6140 72200 2.00 2400 2000 1100 3100 112 Angoia;w Imtrasla oyemenais") 04A8 4337 721 050 5416 34.62 3482 1122 1122 118 lonye;w tenbvasia truncata (caterpillars) 020 2480 2170 4630 020 740 780 780 3880 083 Congo:w oyemens(z"! 020 2120 2310 020 4530 060 5.60 620 570 2560 09 140 040 4340 031 Congorw

‘Samia ricind (prepupse on castor 23645 391 3036 190 1657 wet 562 4526 5088 044 India;

‘Samia ricind \prepupse on tapioca sz 34 346 183 1832 2015 610 4152 4762 046 India;

‘Samia ricindi (pupae on castor leaves”? 7675 S61 3236 178 1619 795 4983 4474 4867 048 India;r Samia zleinf |pupae on taoioca leaves]"' 77118 %4 3263 179 1846 2025 529 4138 4667 049 India:r

074 288 2277 074 2890 31.63 798 167 7218 2985 043 Review Orthoptera (crickets, grasshoppers) 32.05 2337 3708 G48 Achata 3650 30.10 3110 060 Thallandrw Achata confirmata Waterđ 7610 55D 120 3280 240 3110 3450 3220 170 nd ng na 3390 043° Thailand; w Brachytrupes portentosus Lichtenstein? od 181 1579 013 1753 G71 3⁄40 an nd 13 S043 nd S837 060 Thailand; w Brachytrupes ponentosus” 35.00 32.30 28680 O57 Thailand w Chondracis roseapbrunner Uvarov” 1820 640 190 2650 100 2020 21.20 1230 4010 nd "ng nd 5240 036 Thailand; w Gryliotaipa africana Beauvois ms 560 120 3540 480 4560 soso 1380 050 = nd nd nd 1430 055 Thailand w

ose 0893 2759 689 028 3476 4563 1619 058 6240 GƠI Camer;w Ruspolia đif%erens |greenl"! 030 3150 550 040 3830 190 2460 2650 31.20 320 3440 063 Kenya;w Rưapolia difterens (brown? 070 3210 $90 020 3890 140 2490 2630 2950 420 3370 06% Kenya;w Onhoptera? 208 2854 838 204 3896 299 3152 3451 1168 837 2066 071 Review Dictyoptera (cockroaches and termites)

Dictyoptera” 233 11596 411 331 41227 728 4230 4958 106 — nd 106 G81 Review

Descriptive Statistics: SFA, MUFA, PUFA, SFA/PUFA

Statistics

Variable N N* Mean SE Mean StDev Variance Minimum Q1 Median Q3 Maximum

SFA 75 0 3632 144 12.44 154.66 0.59 3036 3482 40.90 95.77 MUFA 75 0 33.33 2.13 1844 340.14 0.72 20.15 34.62 47.42 73.10 PUFA 75 0 3024 2.10 18.20 331.16 2.78 14.30 29.90 43.80 68.80 SFA/PUFA 75 0 0.891 0.296 2563 6.570 0.010 0430 0.530 0.710 22.640

Histogram (with Normal Curve) of SFA Histogram (with Normal Curve) of MUFA

Histogram (with Normal Curve) of PUFA Histogram (with Normal Curve) of SFA/PUFA

All variances are equal

At least one variance is different

a =0.05 95% Bonferroni Confidence Intervals for Standard Deviations

Coleoptera (beetles, grubs) 21 16.5352 (6.46851, 48.478) Diptera 4 2.5129 (0.19062, 101.157) Hemenoptera (bees, ants) 11 5.9444 (3.70511, 12.625)

Hemiptera (True bugs) 6 15.5889 (5.02439, 87.676)

Isoptera (termites) 5 6.3661 (1.58130, 55.476) Lepidoptera (butterfiles, moths) 17 10.2810 (5.60293, 22.412) Orthoptera (crickets, grasshoppers) 11 11.0661 (2.13474, 75.935) Individual confidence level = 99.2857%

II Kiểm định phương sai không có sự khác biệt

II Phương sai cân bằng

One-way ANOVA: SFA versus Edible insect

Method

Null hypothesis All means are equal

Alternative hypothesis Not all means are equal

Edible insect 6 1311 218.6 1.47 0.203 Error 68 10133 149.0

Hemenoptera (bees, ants) 11

Hemiptera (True bugs) 6

Isoptera (termites) 5

Lepidoptera (butterfiles, moths) 17

Orthoptera (crickets, grasshoppers) 11

29.88 43.89 41.97 37.04 32.05

10.28 11.07

(22.53, 37.22) (33.94, 53.83) (31.08, 52.86) (31.13, 42.95) (24.70, 39.39)

Pooled StDev = 12.2075

Tukey Pairwise Comparisons

Grouping Information Using the Tukey Method and 95% Confidence

Edible insect N Mean Grouping

Hemiptera (True bugs) 6 43.89 A

Isoptera (termites) 5 41.97 A

Coleoptera (beetles, grubs) 21 38.49 A

Lepidoptera (butterfiles, moths) 17 37.04 A

Orthoptera (crickets, 113205 A

grasshoppers)

Hemenoptera (bees, ants) 11 29.88 A

Means that do not share a letter are significantly different

Tukey Simultaneous Tests for Differences of Means

Hemiptera (T-Coleoptera( 5.40 5.65 22.58) 0.96 0.962

Isoptera (te - Coleoptera (

Lepidoptera - Isoptera (te

Orthoptera ( - Isoptera (te

-1.92

-6.85

-11.84 -4.93 -9,92

7.39

(-14.99, 21.95) (-13.56, 10.66) (-20.25, 7.38) (-24.81, 18.53) (-13.09, 34.83) (-15.95, 33.85) (-16.61, 24.65) (-22.64, 20.70)

(-4.83, 32.85)

(-7.93, 32.11) (-7.20, 21.52) (-13.66, 18.00) (-24.39, 20.56) (-24.48, 10.78)

(-30.68, 7.00)

(-23.81, 13.95) (-29.94,

0.57

-0.36 -1.42

-0.26

-1.18 -1.91

-0.79

-1.51

0.997

1.000 0.791 0.999

1.000

0.899 0.480 0.985 0.740

10.10)

Orthoptera ( - Lepidoptera -4.99 472 (-1935,937) -1.0G Individual confidence level = 99.66%

Lopidoptera - Isoptera íte+

Oxrtheptera ( - Isoptera (te —— _I— —_

Orthoptera (- Lepidoptera)

fon interval does net contain zero, the corresponding meons ore significantly diferent

Fisher Pairwise Comparisons

Grouping Information Using the Fisher LSD Method and 95% Confidence

Edible insect N Mean Grouping

Hemiptera (True bugs) 6 43.89 A

Isoptera (termites) 5 41.97 A B

Coleoptera (beetles, grubs) 213849 A B

Lepidoptera (butterfiles, moths) 1737.04 A B

Orthoptera (crickets, 113205 A B

grasshoppers)

Hemenoptera (bees, ants) 11 29.88 B

Means that do not share a letter are significantly different

0.938

Lepidoptera - Isoptera (te

Orthoptera ( - Isoptera (te

-5.47 -8.61 5.40 3.48 -1.45 -6.44 -3.14

10.87 8.95 4.02

-0.97

14.01

12.09 7.16

-1.92

-6.85 11.84 -4.93 -9,92

6.66 (-18.76, 7.82) 4.54 (-17.67, 0.46) 5.65 (-5.87, 16.68) 6.07 (-8.64, 15.60) 3.98 (-9.40, 6.50) 4.54 (-15.50, 2.63)

7.13 (-17.36, 11.08)

7.88 (-4.86, 26.59) 8.19 (-7.39, 25.29) 6.78 (-9.52, 17.56)

7.13 (-15.19, 13.25)

6.20 (1.65, 26.37)

6.58 (-1.05, 25.23) 4.72 (-2.27, 16.59) 5.21 (-8.22, 12.56)

739 (-16.67, 12.83)

5.80 (-18.42, 4.72) 6.20 (-24.20, 0.52) 6.21 (-17.32, 7.46) 6.58 (-23.06, 3.22)

Adjusted T-Value P-Value

-0.82 0.415 -1.89 0.062 0.96 0.343 0.57 0.568 -0.36 0.717 -1.42 0.161 -0.44 0.661

1.38 0.172 1.09 0.278 0.59 0.556 -0.14 0.892

2.26 0.027

1.84 0.071 1.52 0.134 0.42 0.678 -0.26 0.796

-1.18 0.241 -1.91 0.060 -0.79 0.430 -1.51 0.136

Isoptera (te - Diptera* i >

Lepidoptera - Dipteray e—-_ Tr

Homiptera (T - Homenoptera lL———— Isoptera (te - Hertsertepter a'† +t

Orthoptera (- Isoptera (te cm Ề 8® eo

(Oxtheptera ( - Lepiđeptera| † ——e'—- t +

fan interval does not contain zero, the corresponding means are significantly diferent

95% Cl for the Mean w—c

Probability Piot

Test for Equal Variances: MUFA versus Edible insect

Method

Null hypothesis All variances are equal

Alternative hypothesis At least one variance is different

Tests

T Method est P-Value

Statistic

10

0 Kiém dinh phuong sai khéng c6 su khac biệt

I Phương sai cân bằng

Dipter: eee Oipter mm

Lepidoptera (butterfiles, moths ma cece Lepidoptera (butterfiles, moths) —_

One-way ANOVA: MUFA versus Edible insect

Method

Null hypothesis All means are equal

Alternative hypothesis Not all means are equal

Edible insect 6 6021 1003.6 3.56 0.004 Error 68 19149 281.6

Hemiptera (True bugs) 6 32.39 19.89 (18.72, 46.06)

Isoptera (termites) 5 22.00 18.91 (7.02, 36.97) Lepidoptera (butterfiles, moths) 17 23.36 10.51 (15.24, 31.48) Orthoptera (crickets, grasshoppers) 11 29.37 11.16 (19.27, 39.46)

Pooled StDev = 16.7811

Tukey Pairwise Comparisons

Grouping Information Using the Tukey Method and 95% Confidence

Edible insect N Mean Grouping

Hemenoptera (bees, ants) 1148.76 A

Coleoptera (beetles, grubs) 2135.72 A B

Hemiptera (True bugs)

17 23.36

5 22.00 A B Means that do not share a letter are significantly different

Tukey Simultaneous Tests for Differences of Means

Lepidoptera - Coleoptera( -12.36 547 (-29.00, 4.29) -226 0.280

-25.34 Orthoptera ( - Coleoptera( -6.35 6.25 " 64) -102 0.949

Lepidoptera - Isoptera (te 1.36 8.54 ( 27.32) 0.16 1.000

Orthoptera optera ( - Isoptera (te ra ( - ( - Isoptera T: (t 7.37 9.05 (20.15, 34,89) 0.81 0.983

Lepidoptera - Isoptera (te eg T5

Orthoptera (- soptera (te a

Orthoptera (- Lepidoptera { ———k——»——— T

“SO “28 oO 23 S0 (fan interval does net contain zero, the corresponding means ore significantly diferent