Luận văn thạc sĩ Kỹ thuật hóa học: Nghiên cứu trích ly hoạt chất từ nấm linh chi và thử nghiệm hoạt tính sinh học của cao trích

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(Extraction of biological activities from Ganoderma lucidum

and examination of bioactivities)

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7rQWLӃng Anh: Extraction of biological activities from Ganoderma lucidum

and examination of bioactivities

II NHIӊM VӨ 9¬1ӜI DUNG:

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ABSTRACT

In this thesis, triterpenoid was extracted from Vietnamese Ganoderma lucidum (G

lucidum) by the ultrasonic-assisted enzyme extraction (UAEE) method The

triterpenoid content was determined by ultraviolet±visible spectroscopy (UV±Vis)

Chemical composition and heavy metal content in G lucidum have been investigated, showing that G lucidum has high fiber, carbohydrate and lipid content

In addition, the content of heavy metals such as As, Cd, Hg and Pb was within the allowable limit

The influence of each extraction condition such as enzyme concentration, etanol:material ratio, temperature, time and power on triterpenoid content was investigated by the method of alternating each variable to find a suitable range of variation Then, conducting a simultaneous survey by experimental planning using the Plackett±Burman and Box±Behnken designs The data were processed using Design Expert 11.0 software to find extraction conditions for the highest triterpenoid content

At the same time, the characteristic functional groups of the extract, the morphology of the surface structure of the fungus and the high composition of the extract were investigated by Fourier transform infrared spectroscopy (Fourier transform infrared spectroscopy ± FTIR), scanning electron microscopy (scan electron microscopy ± SEM), and gas chromatography ± mass spectrometry (GC±MS)

The extract at optimal conditions was tested for biological activity through antitumor activity on three human cancer cell lines (Hep-G2 and MCF-7) and the

antibacterial ability of triterpenoids was tested on Escherichia coli, Staphylococcus

aureus, Pseudomonas aeruginosa, and Salmonella enterica by antibacterial ring

diameter measurement

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1.1.1 Giͣi thi͏u chung 2

1.5.4 ĈyQJJySFͯa lu̵QYăQ 28

THӴC NGHIӊM 29

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2.2.1 Kh̫RViWWKjQKSK̯QKRiK͕c trong ṋm linh chi 30

2.2.2 Kh̫RViWV͹ ̫QKK˱ͧng cͯDFiF\͇u t͙ ÿ͇QKjPO˱ͫng triterpenoid 33

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3.2 ҦQKKѭӣng cӫDFiF\Ӄu tӕ ÿӃQKjPOѭӧng triterpenoid 46

7¬,/,ӊU THAM KHҦO 102

PHӨ LӨC 112

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+uQK&iFWULWHUSHQRLGWKXӝFQKyPĮ-amyrin 6

+uQK&iFWULWerpenoid thuӝFQKyPȕ amyrin 7

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+uQK&ѫFKӃ NKiQJR[\KyDEҵQJFiFKFKRÿLӋn tӱ 9

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+uQK1JX\rQWҳc thӱ nghiӋm hoҥWWtQKNKiQJXQJWKѭ 26

+uQK4X\WUuQKWUtFK ly triterpenoid tӯ nҩm linh chi bҵQJSKѭѫQJSKiSenzyme kӃt hӧSVLrXkP 34

+uQKPhә UV ± YLVYjÿѭӡng cong chuҭn cӫa axit ursolic ӣ ɉ݉ܽݔ ± 548 nm 41

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+uQKҦQKKѭӣng cӫa tӹ lӋ dung P{LQJX\rQOLӋXÿӃQKjPOѭӧng triterpenoid 48

+uQKҦQKKѭӣng cӫa nhiӋWÿӝ VLrXkPÿӃQKjPOѭӧng triterpenoid 49

+uQKҦQKKѭӣng cӫa thӡLJLDQVLrXkPÿӃQKjPOѭӧng triterpenoid 50

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