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CÁC PHƯƠNG PHÁP CHIẾT XUẤT VÀ CHUẨN BỊ MẪU DƯỢC LIỆU PHƯƠNG PHÁP ĐỊNH LƯỢNG CÁC NHĨM HỢP CHẤT CHÍNH
CÁC PHƯƠNG PHÁP CHIẾT XUẤT VÀ CHUẨN BỊ MẪU DƯỢC LIỆU PHƯƠNG PHÁP ĐỊNH LƯỢNG CÁC NHĨM HỢP CHẤT CHÍNH Tài liệu tham khảo 1/- J Azmir et al Techniques for extraction of bioactive compounds from plant materials: A review / Journal of Food Engineering 117 (2013) 426–436 2/- Hans - J ö rg Bart (2011 ) Extraction of Natural Products from Plants – An Introduction Published 2011 by Wiley-VCH Verlag GmbH & Co KGaA 3/- S Sasidharan,1 Y Chen,1 D Saravanan,2 K M Sundram,3 and L Yoga Latha1 (2011) Extraction, Isolation and Characterization of Bioactive Compounds from Plants' ExtractsAfr J Tradit Complement Altern Med 2011; 8(1): 1–10 Published online 2010 Oct http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3218439/ 4/- Brusottia,b,∗, I Cesaria,b, A Dentamaroa,b, G Caccialanzaa,b, G Massolinia, (2014)Review of Isolation and characterization of bioactive compounds from plant resources:The role of analysis in the ethnopharmacological approachG Journal of Pharmaceutical and Biomedical Analysis 87 (2014) 218– 228 5/Dr SINDHU K M V Sc SCHOLAR, DEPT OF VPT, COVAS, POOKODE.; PHYTOCHEMICAL EXTRACTIONhttp://www.slideshare.net/Sindhukuberappa/phytochemical-extraction?related=1 Các bước khảo sát dược liệu PHYTOCHEMICAL EXTRACTION The general techniques of medicinal plant extraction • • • • • • • • • • • • maceration, infusion, percolation, digestion, decoction, hot continuous extraction (Soxhlet), aqueous-alcoholic extraction by fermentation, counter-current extraction, microwave-assisted extraction, ultrasound extraction (sonication), supercritical fluid extraction, phytonic extraction (with hydrofluorocarbon solvents) Modern Extraction & Separation Techniques •Liquified gas extraction •Supercritical fluid extraction •Protoplast technique •Membrane extraction •Solid phase micro-extraction (SPME) •Headspace trapping techniques —Static headspace —Dynamic headspace —Vacuum headspace •Controlled instantaneous decomposition (CID) •Simultaneous distillation extraction (SDE) •Microwave extraction & distillation •Microdistillation •Molecular spinning band distillation •Thermomicrodistillation •Preparative fractionation Essential Oil Extraction Techniques CÁC KỸ THUẬT CHIẾT XUẤT CÁC KỸ THUẬT CHIẾT XUẤT •Expression or Cold Pressing is confined to citrus oils •It involves inducing physical damage to the essential oil glands on the surface of citrus fruits to release the oil •It is simultaneously washed by the passing water and recovered using an oil-water separator 10 ... decoction, hot continuous extraction (Soxhlet), aqueous-alcoholic extraction by fermentation, counter-current extraction, microwave-assisted extraction, ultrasound extraction (sonication),... supercritical fluid extraction, phytonic extraction (with hydrofluorocarbon solvents) Modern Extraction & Separation Techniques •Liquified gas extraction •Supercritical fluid extraction •Protoplast... MÔI The extraction is done by organic solvent •After the extraction, solvent is evaporated •This “concentrated solution” is then directed, after filtering, into a vacuum evaporator •Vacuum distillation