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MINISTRY OF EDUCATION AND TRAINING CAN THO UNIVERSITY SUMMARY OF THE THESIS OF DOCTORS MAJORING CROP SCIENCE CODE 62620110 VO MINH HAI IMPROVING PROPAGATION TECHNIQUES FROM EMBRYOS AND METHODS TO INCR[.]
MINISTRY OF EDUCATION AND TRAINING CAN THO UNIVERSITY SUMMARY OF THE THESIS OF DOCTORS MAJORING: CROP SCIENCE CODE: 62620110 VO MINH HAI IMPROVING PROPAGATION TECHNIQUES FROM EMBRYOS AND METHODS TO INCREASE THE PERCENTAGE OF SAP FRUIT OF THE SAP COCONUTS IN TRA VINH PROVINCE CAN THO, 2022 WORK WAS COMPLETED IN CAN THO UNIVERSITY Main instructor: Assoc Prof Dr Nguyen Bao Toan – Can Tho City Ornamental Creature Association Assistant instructor: Assoc Prof Dr Le Vinh Thuc – Department of Crop Science, Faculty of Agriculture, Can Tho University The thesis is defended by the university-level doctoral thesis grading committee Meet at: ………………………………………… At …………h…………day……month… 2022 Reviewer 1: …………………………… Reviewer 2:…………………………… Thesis can be found at the library: - Learning Resource Center, Can Tho University - Vietnam National Library LIST OF PUBLICATIONS [1] Võ Minh Hải nh h c h m h h ng hu uy ng guy n o o n 2020 i u qu t i ch nh tr ng d a s p v d a th ng tr n đ a b n huy n ầu Kè tỉnh r inh” Tạp chí Khoa học & Cơng nghệ Nông Nghiệp, đại học Nông Lâm Huế, tập 4(2), tr.1897-1906 [2] Võ Minh Hải h m h h ng hu rần h h o ang nh h c guy n o o n 2020 ghi n c u m t s y u t nu i c y in vitro nh h ng t i qu tr nh n y mầm ph i d a s p Tạp chí Nơng nghiệp Phát triển Nông thôn, Bộ Nông nghiệp Phát triển Nông thôn, tr 3441 [3] Võ Minh Hải h m h h ng hu nh h c v guy n o o n 2021 ghi n c u nh h ng c a m t s y u t nu i c y in-vitro đ n qu tr nh t o r c y d a s p a apuno coconut c y ph i Tạp chí Khoa học Trường Đại học Cần Thơ, 57(2), 127-134 [4] VO MINH HAI, Toan Nguyen Bao, Thuc Le Vinh, Thuy Pham Thi Phuong, 2022 omparing the Economic Efficiency and echnical Aspects affecting the Performance of Both Sap (Makapuno) Coconut odels in ietnam in ase at inh rovince” International Journal of Science and Research 11(5), 1203-1212 Chapter : INTRODUCTION 1.1 The main reseon of the thesis Tra Vinh is a coastal province in the Mekong Delta with many specialty fruit trees, in which sap coconut is a specialty that makes the famous of Tra Vinh province at Vietnam and abroad, because sap coconut has many special characteristics with outstanding features such as: delicious, unique, strange and brougth more than 10-20 times income for farmers growth sap coconuts compared normal coconut (Pham Thi Phuong Thuy et al., 2016) The sap coconut endosperm is delicious because the total hydrolyzed polysaccharide content of sap coconut water is 30 times higher than that of normal coconut and when fractionated the polysaccharide component from sap copra showed that the soluble Galactomanan content was 2-8 times higher times compared to normal coconut, in sap copra had 12 amino acids and compared with amino acids changed in normal coconut copra In addition to eating fresh, sap coconut to produce a product called "Mak gum" used for many industries of food processing (food film), pharmaceuticals (medical film, wound gauze, ingredients) in agarose and polyacrylamide gels) and cosmetics (Maria et al., 2003) Research on improving the yield of sap coconuts was focused on (1) Embryo culture, sometimes called embryo rescue; (2) Cultivation techniques affecting yield and quality of sap coconut include fertilizers and fertilization techniques to help improve fruit yield (Vo Van Long, 2007) For the research on solutions to create seedlings, there were many factors affecting the low success rate such as: 10-15% weak germinated embryos, 13-17% of plants had poorly developed roots and young seedlings dead from 9-14% (Pham Thi Phuong Thuy et al., 2016) Therefore, the thesis continues to research on stimulating the development of weak germinating embryos and plants with poorly developed roots in order to improve the success rate of embryo cuture For the solution to research on farming techniques, the thesis chooses to study the effects of fertilizer dosage and type of fertilizer on yield and quality of sap coconuts based on the fertilizer formula of normal sap coconut trees was recommended by Research Institute for Oil and Oil trees of Vietnam Research solution to improve the rate of pollination and fruit set, the thesis chooses to study the method of flower crowning and spraying with Boric acid to help increase self-pollination and increase the percentage of sap coconut fruits on the bunch of groups: Dwarf sap coconut and hybrid sap coconut According to Hanson and Proebsling (1996) flowers had a high need for Bo during bloom for fruit formation Spraying of Bo on flowers was intended to facilitate fertilization, fruit formation and fruit development at an early stage Solar and Štampar 2001 Form the above problems, The thesis "Improving propagation techniques from embryos and methods to increase the percentage of sap fruit of the sap coconuts in Tra Vinh province" 1.2 Research objectives - Improve the success rate of sap coconut propagation by embryo culture method - Improving yields and percentage of sap coconut fruits with embryo cultured sap coconut by cultivation techniques in Tra Vinh province - Assess the current status of factors affecting the rate of sap fruit and compare the profitability of normal coconut models and embryo cultured sap coconut models in Tra Vinh province 1.3 Expected results - Increasing the success rate (embryo/seedling) in the propagation of sap coconut by embryos compared to the present (currently