Thông tin tài liệu
r Nei-lll':N C:i/U - l/NG OUNeS Xay dung mo hinh toan hoc mo ta SU phu thuoc giua san pham thiiy phan hot ngo vol cac yeu to anh huong den qua trinh sii dung che pham enzim PHAM THI THANH HUYEN Trudng Cao ddng Cdng nghiep Thuc phdm Tinh hot Id nguyin lieu de sdn xudt nhiiu loai sdn phdm thUc phdm do, phdn itng thuy phdn tinh hot Id mot mdt xich quan trong quy trinh che Men, quyet dinh hiiu sudt, chdt lUOng vd ddc tinh sdn phdm Di tdi "Xdy dUng mo hinh todn hpc mo td sU phu thuoc giUa sdn phdm thuy phdn hot ngd vdi cdc yiu to dnh hudng tdi qua trinh sit dung che phdm enzim", Id nghien citu sit dung cong cu todn hoc di diiu chinh cO cdu thdnh phdn cua sdn phdm thuy phdn, tao hon hop cdc cdu tit dudng phu hpp cho tUng muc dich sd dung, nhdm tqo dieu kien da dang hod sdn pham vd tqo sdn phdm nhUy Nguyen Uiu - Bdt ngd te (loai ngd vang), dupc xac dinh ham lucmg tinh hot ciia nguyen lieu - Che pham enzim fungamyl 800L(alpha-l,4-glucan-4-glucanohydrolase, EC 3.2.1.1) va AMG (a-1,4 glucan, 4-glucoliydrolase Ec 3.2.1.3) cua Novo - Dan Mach, da duoc xac dinh lai hoat luc Phuang phdp - Ham lupng dextrin: phUPng phdp ket Ilia cdn [1,2]; • Ham Itfdng dtrdng maltoza: phuong phap lot [1,2]; - Hdm lucmg glucoza: phuong phap DNS [1,21; - Xay dtfng md hinh todn hoe dang ham ndi suy: phUemg phdp binh phuong cifc tieu |3,4|; Phifemg phdp quy hoach thuc Website: www.tapchicongnghiep.vn nghiem: Box willson [3,4| II Ket qua Khdo sdt ddng thdi cua qud trinh dudng hda bar ngd bdng che pham fungamyl 8001 Trong nghien cifu ndy, bdt ngd dupc hd hda vd dich hda theo cac dieiu kien cdng nghe da dupc ngliien cifu tii trUdc Sau kill tham khio cac tai lieu, chung tdi chpn cac mdc gid tri pH=5; thdi gian 24 gid; ndng dd chit khd 24%, lieu lucmg che pham enzun 0,1% (so veii chit khd ciia nguyen lieu) de tien hanh dueing hod vdi che pham enzim Fungamyl Nliieii dd dtfpc kliao sat tai cac gid tri 43; 30; 55; 60"C Ket qua thi nghiem dupc bieu dien tren dd llii Khao sdt iinJi hucing ctia theii gian giu nhiet dd d ji'^C, thcii gian thay ddi t;ii cdc gia tri d, 12, irv.moit.gov.vn 18, 24, 30, 36 gid Cac y eu td cdn lai dp dung nhu 1.1 Co cdu s;m pham nhin ducJc theo thcii gian thuy phdn dupc ttinh bay d dd thi * dU ^ Q^.m.i —•— maR02a LWJcirir 7ri uong{%) I Phtfdng phap nghifin culi ^"J 20 lU / ^\ * >>'^^ ^ u'i =iri '^.'^ f.- \ • ' • ^ wiiet ao loci Do thi 1: Anh hudng cua nhiet den ca cdu thdnh phdn dich thuy phdn 60 ã.*.> f: -.-r^l***! -m- 9iônn U^ôằi / ^-ã" e 12 1! ?i 'Thai « \ 90 36 Do thi 2: inh hUinni cua l/icl cfuin den ca cdu thdnh phdn dich thuy pluin Khoa hpc & Cpng ngh$ [6/2009] >il;iHiH,i 4B Vl\ NGNir=N ClAJ • l/NG DUNG Khao sdt ndng dd enzim dupc tien hanh v di cac gia tn 0,03; 0,1; 0,15; 0,2% so vcii ham lueing chit klid ciia nguyen lieu Ket qui dupc the hien tren dd thi Ket qui khio sat cho thay, ci yeu td deu cd inh hueing manh teii cP cau sin pham thuy phan Qua dd da xac dinh duoc cac gieii han cua cdc bien quy hoach thuc nghiem nhtf sau: Lieu lupng che pham enzim: x, G [0.05 H- 0.15] 'it; Nhiet dd dudng hod: x^ e [50 ^ 60], "C; Thdi gian dueing hoa: X3 G [12 ^36], gid Do thi 3: Anh hudng cua nong che phdm den cocdu sdn phdm dich thuy phdn Xdy dung md hinh todn hoc ciia qud trinh dudng hda hot ngd sii dung chi phdm fungamyl Dei xay dung md hinh todn hoc md ti su phu thuoc gitia ty le glucoza, maltoza, dextrin veii cdc yeu td dueic xem xet tren, chung tdi chon phUemg phdp binh phuong tdi thieu [3] Dat md hinh todn hpc cho tiing hdm muc lieu cd dang: il = ty le glucoza (%) = a' | Xi+ a'^x^ + a' ^x^ (1) i;= ty' le maltoza (%) = a-1X1+ a-_,X2 + a-^x^ (2) V, = ty le dextrin (%) = a'lXj^ ^-^i>^i + ^'j^.i (3) •'••• Xay dtmg ke hoach thuc nghieim vd tien hdnh thi nghiem nhan thdng lin Keit qui thuc nghiem theo ma trdn dtfpc trinh bav d bing Bdng 1: Kit qud thUc nghiem theo ma trdn 2^ vd thim thi nghiim md rdng Ciia In bien {hifc So IN X, « 0,05 i i; i «,I5 MS 0.05 10 OJS 50 SO 60 M 50 Si 60 0.05 •J5 12' ! II 0.1 u 0.1 to 13 0.1 " IM 0.1 55 St 60 91 55 S5 IS COS 0.05 u Oil 17 0,05 u ã,15 ! 12 "12 "12 "12 ô 12 u « 12 16 { 3C ,16 3< 1' X + * * 12 1 • * X, »i + 5,6 M 4,4 30 + \% 7^1 Tnii j fi.! y a OJ IM lli,2 16J 16J M 0,1 15,8 I6J 16,1 16,0 W 20 ni\ Ofi n» «,«7 0,1 19,6 H,S W,6 19,6 0,01 'iSi x,'C - M i 12 I M 4,4 u ei* M I 3t j S4 (M S,7 8,9 { 8,9 ; 8,9 + 4,4 OJ 4,6 4j • 5^ j U { 54 0.1 IV I2,l| 12,412,4 + 4,4 7,9 + • TyieDextiin,X 30 12 54 54 OJ 8,1 4,2 7,9 4,4 8,0 UM •S' m 0,02 IMI2 0,M w 0,01 V* NGI-llfEN Cl/U - l/NG DUNG Fishe Ta cd chudn theo tinh loan ttf mo liinh iF-j-,) veil N=S, B=5 la 4,6; chuan tisher tra bing (F5)= 5,20 \'ay: F.pj < Fj^: md hinh da xdy difng Id tliich Ung ChUng tdi tdi tm hod dieu kien dueing hoa bang dudng thuc nghiem Ma trdn thuc nghieim tdi tm hod dupc bdt dau bang lhi nghiem d mUc co ban chei tdt ci cdc bien: [x = 0: X = 5.0]: [.•;•, = 0; x, = 60]: [.i.= .V: = 21]: [x^ = 0: V; = 0.2] veil budc nhiv eua cdc bien: : 0.21 [i =0:x = 5.0]: [i =0: x, = 60]: [.i- = 0: v = 21]: [i, =0;x, Bdng 4: Kit qud thUc nghiim qud trinh tdi Uu cue tiiu hod ty ii dextrin dUdng hda bdng chi phdm AMG Ty le dextrin va glucoza TT X, ».- K'"* X, ' C X , 5,0 4,9 2< 22 43 4,7 0.20 * Dextrin e%chit (ihicoza (%chat h o a tan Ihu hoi) h o a tan Ihu hoi) 0.22 6.2 4.8 23 60 59 58 •.M 3.0 24 57 0,26 3,1 '•.a 25 56 26 55 0.28 0.30 3." 4.5 i -M 80,7 83,6 96,1 86,6 «M 86.4 (Jua sei lieu bdng cho lhd>, tU lhi iighicm Ihu -i trd di, ty le dextrin tremg dich thuy phan hau nhu Ididng giim, chimg td gieii han dueii cudi cung cua dai lifemg ndy Id khoing 3,0% Tifemg tfng veil inUc bc nhat ctia ty le dextrin la ty lei cao nhdt cua gluceiza dat 8(),7/e, Cdc dieu kien tdi uli tuemg img la pH = 4,8, theii gian dUimg hoa 23 gio, nhie''t dd 58"C lieu lUemg AMG 0,24'/'.) lhi ty le dextrin cua dich difemg hod dat mUc thdp nhdt la 3,0% Kieiin tra suhdi tu cua sd lieu bang chuan Cocren Ta cd chuaii Cocren theo tinh loan (G.j-|)= 0,3 Chuan Cocren tra bing (Gjj^-^g) vdi ngifdng sai sd a= 0,03 la 0,56 vdy Gy.^.< Gi^^j^jj, sd lieu thu duo'c d bang dupc chap nhan III KIT LUAN Da khio sat s u i n h hueing ciia thdng sd nhiet PhUPng trinh >• = &„ +^,-?-, +i>2^'i +b-^x^ ^^'^x^ dd, lheii gian vd lieu luong che pham enzim anh lc dextrin vd yeiu td xem xet si.y ifpc xdy dung theo hueing dein co cdu s i n p h i m thuv phdn bdt ngd dang luyc'n tinh: sir dung chei pham enzim Fungam>l 800L vd anli Trong dd bj (i= ^4) Id he sd; Id bicn sd ma hda cua lhdng sd la nliic't do, iheti gian va lit'u hue'mg cua cdc bien lucmg che phdm enzim, pH i n h hueing den ca cau l h e o phucmg phdp da dan, ta tuih dupc: b() = s i n p h i m thti> phan bdt ngd sir dung che; pham 13,2; b j ^ k l ; b = - l , ; b3= 4,7; b4=-1,1 enzim \MG Cu the, da xdc dinh duoc gid lri v a cdc Kieiin tra sU cd nghia eua cdc he sd bang chuan dieu kien cdng nghe dp dung che pham tren Student: Sau tinh j/x UPng sai; chudn Student tra bang vcii N=8, bac tu 1^= N (k-1) = 8(3-1) =16, ta Da xay dtmg dupc md hinh loan hoc md la qud dUPc t = 2,12 Ta cd > S^ x I = 0,0848 (i=l ^5) lrinh thuy phdn bien ddi nguyen heu bdt ngd bang Vdy tdt ci cdc hei sd cua phucmg trinli (5) deu cd chci pham enzim Fulgamv 80()L lhdnh cdc sin pham nghia Phifemg trinh hdi quv md td sir phu thudc gitfa glucoza, maltoza vd dextrin nhU sau: ty le dextrin vd >eu id anh hueing dang xet Id: 'y, = 40,75x, - 0.056x_ - 0,\A6\, V = 13.2 + 1.1 X - l.K x , - , 1 (5) Kie"'iii tra sU thich ihig cua md hmh (5) thee) chudn , y^ = 127,04xj - 0.63.x, - 0,36x, •y, =-99.5x, -0.79x, -0,3:.x, i\cm tiep trang 31) Website: wwwtapchicongnghiep.vn irv.moitgov.vn Khoa hoc & Cong nghe [6/2009] * < i ; i M i H J 47 r hien "That ra, hoat dpng cdng chung d Vict Nam da cd ttf nam 1931, nhieu ly nen den nam 1991 meii "kheii ddng" lai, nhtmg thuc chat den nam 1996, Chinh phu meii ban hanh Nghi dinli 31/CP ve td chtfc va boat ddng ciia cdng chtmg Nhd nueic de thay the Nghi dinh 45/HDBT (nam 1991) Nhung nhieu bat cap, Chinh phii lai ban hdnh Nghi dinh 75/CP ngdy thang 12 ndm 2000 (cd hieu luc tu ngdy thdng nam 2001) vei cdng chifng, chUiig thuc dc thay the Nghi dtnh 31/CP Tiep dd, ngdy 14 thdng nam 2001, Bd Tu phdp ban hanh Thdng tU 03/2001/TP-CC de hucing ddn thuc hien Nghi dinh 75/CP Qua nghien ctfu thi thay, ND 75/CP cdn nhieu diem bat cap, chua ro rang, lham chi Ididng chinh xdc, gay klid khdn, lung tUng boat deing cdng chung (ddy ctmg Id cdn benh mudn thiia cua ludt phdp ntfeic la) Nhtmg xin chi neu mdl van de rat nhd Theo ND 75/CP thi "cdng chtfng" dUeJc hieu Id viec Phdng Cdng chtmg xdc (chtmg) nhin tinh xdc lhtfc ciia hpp ddng dupc giao ket hodc giao dich khdc dUpc xdc lap quan he ddn su, kuih te, thuong mai v d quan he xa hdi khdc Cling nhu thuc hiein cdc viec khdc lheo quy dinli ciia phdp luat Cdn "chtmg lhuc" la viec UBND cap hu>'en, xd xac nhdn y gia>' tei, help ddng, giao dich va chu ky ctia cd nhan cdc giay Id, phuc \\i cho viec thUc hiem cdc giao dich cua ho theo quy dtnh ctia phdp ludt Nhu thei', Iheo tinh than ND 75/CP su(cd gang) phdn dtnh cdng chung vdi chung thuc chi dtfa \do lieu chi nhat la chu the cd tham quye''n thuc hitin chimg nhan dc phan dinh la khdng chinh xdc i hcei dung khoa hex' phap ly, thi dc' Website: www.tapchicongnghiep.vn ldm rd hoai ddng ndo la cdng chung, boat ddng ndo la chiing thuc, phai duci vao ban chat cua hoat ddng ay Xet ve khia canh luat, thi bin chat ciia hoat ddng cdng chung la thdng qua sp chung nlidn ciia CO quan cdng quyen, lam cho cac van bin, giay td trd nen cd dd tin cdy cao hOn so vdi cdc van bin, giay td chua dupc cdng chiing; hav ndi mdt cdch khac, la dem lai cho cdc van bin, gidy td "ddu an" cua cdng quyen Ttf cdch hieu dd, nhieu ngucii cho rang, nhtfng gi duOc goi Id "chimg lhuc" nhu quy dinh lai Dieiu Nghi dmh 75/CP, xet vc ban chdl phii dupe gpi la "cdng chtfng" Bdi le, xet ve bin chat thi hanh vi cdng chUng (vd ci hdnh VI chtfng thUc cd tinh chdl cdng chtfng nhu quy duih ciia Nghi dinh 75/CP) khac veil hdnh vi thi thuc hanh chinh Thuc ra, ddi tUcftig cua hiinh vi thi lhuc hdnh c:hinh Id nhting van ban gidy td thudc linh vifc qudn ly hdnh chinh (nhu chtmg nhdn tinh trang hdn nhan, chtfng nhdn ly' hch ,); cdn ddi Ufemg cua hdnh vi cdng chUng Id nhiing help ddng, giao dich, gidy td thueic cdc linh vifc dan stf, kinh tc, thuong mai (nhu hop ddng dan su, di chUc ) Nii'u ndi each khdc ciia "ngdn hf" luat, lhi ban chat cua hdnh vi "tlii lhuc hiinh cliinli" Id su nlidn xe;t, dp dat mang tinli chdt hdnh chinh ctia chinh quyen ddi v eii mpt sU kiein phap ly cu the] ndo v a ddi veii cdng dan thudc quyein quin ly ciia minli; cdn ban chat ctia hdnh vi "cdng chtfng" Id viec chtmg nhdn y chi, ngii) en vqng ciia dUPng su, ho lip cdc van bin, giay id phuc cho cdc giao dich ctia ho Tuv nliiein, xudt phdt ttf Ucb stf V d dieu Iden thtfc tc d nudc ta, thi khdng nhit lhiet moi viec cdng chimg deu quy dinh cho Phdng Cdng chimg \ ice ticp luc giao cho l^ly ban nhdn ddn thUc hie"'n mdt sd irv.moitgov.vn VAN I2AN Pi-JyVI' I.UAT viec cdng chifng don gidn phd bien, Id hpp ly, nliung cdn ndi rd nhtfng vic!c dy van phii dUOc goi la cdng chiing, chu khdng vi giao cho Uy ban nhan dan (hoac mdt co quan ndo dd) md ddn dein vice goi khac di la "chung lhuc hav thi thuc" Xung quanh ndi dtmg cua Nglii dinh 75/CP cdn nliieiu van de kliien ngueii ddn phdn ndn Thiet nghi, can ldm ro rang, minh bach de ngudi ddn dd klid, vi de nhu v dy, cdc "quan" se cd cd de "hdnli" dan la "chinh" dimg duoc md hinh todn hoc md td qua trinh thuy phan bien ddi nguy en heu ngd bing enzim AA\C de sin pham Ihuy phdn cd hdm lupng glucoza cao nhdt (dextrin thdp nhdt) nhu sau: y = 1.1.2 * 1,1 t , - l x ; , - , ; , - , f, (5) Da xac dinh duoc cdc dieu kiiin cdng nghe teii lai: pH = 4,8, thdi gian dudng hod 23 gid, nhiet dd 58"C, lic'u luemg \MG 0,24% thi ly le dextrin ctia dich dudng hod dat muc thdp n h i t Id 3,0% IV TAILIEU THAM KHAO .\0.\Q\ Ollicial Methods ol Analysts of \0\Q International, l()*cd., USA, 199« Le Thanh Mai (chu bien) (2006), Cejc phuang phdp phdn tich ngdnh cong nghc len men, NXB Khoa heic \a ky thual, lii N61 Biu MLih Tn (200S), Xeic sudt thong kc vd quy hoach thuc nghiem, NXB Khoa hoc va ky thuat, I lu Neii Nguv'en Minh Tuyi^n (211(14), Quy hoach thit nghiem, NXB Khoa ^ hoc va ky Uiuat, Ha Not Khoa hoc & Cong ngh$ [6/2009] k'.tllUMi gi ... DUNG Khao sdt ndng dd enzim dupc tien hanh v di cac gia tn 0,03; 0,1; 0,15; 0,2% so vcii ham lueing chit klid ciia nguyen lieu Ket qui dupc the hien tren dd thi Ket qui khio sat cho thay, ci... dupc chap nhan III KIT LUAN Da khio sat s u i n h hueing ciia thdng sd nhiet PhUPng trinh >• = &„ +^,-?-, +i>2^''i +b-^x^ ^^''^x^ dd, lheii gian vd lieu luong che pham enzim anh lc dextrin vd yeiu... sir dung chei pham enzim Fungam>l 800L vd anli Trong dd bj (i= ^4) Id he sd; Id bicn sd ma hda cua lhdng sd la nliic''t do, iheti gian va lit''u hue''mg cua cdc bien lucmg che phdm enzim, pH i n h
Ngày đăng: 08/12/2022, 21:21
Xem thêm:
