Phụ lục hướng dẫn KẾT QUẢ HOẠT ĐỘNG KHOA HỌC VÀ CÔNG NGHỆ CÔNG BỐ NĂM 2018 Số TT Nội dung Đơn vị chủ trì, Tác giả Tóm tắt nội dung Ghi chú I SÁCH 1 Quản lý sức khoẻ tôm nuôi nước lợ Đặng Thị Lụa, Trươ[.]
KẾT QUẢ HOẠT ĐỘNG KHOA HỌC VÀ CÔNG NGHỆ CÔNG BỐ NĂM 2018 Số TT I Nội dung Đơn vị chủ trì, Tác giả Tóm tắt nội dung Ghi SÁCH Quản lý sức khoẻ tôm nuôi nước lợ Đặng Thị Lụa, Trương Thị Mỹ Hạnh, Phan Thị Vân Nhà xuất Nông nghiệp (2018) BÀI BÁO QUỐC TẾ Species identification using DNA barcoding on processed Panga catfish products in Viet Nam revealed important mislabeling Identification and analysis of SNPs in population of Vietnamese catfish (Pangasianodon hypophthalmus), using next generation sequencing and SNP validation Tran Thi Thuy Ha, Nguyen Thi Huong, Nguyen Phuc Hung, Yann Guiguen Nucleotide sequences of the Cytochrome oxidase subunit I gene and Cytochrome b were analyzed for 10 processed fish products collected from supermarkets in Hanoi, Viet Nam The similarity between our results and published data from the NCBI and BOLD was compared to identify species This molecular analysis showed that the common names of only of these products matched with their corresponding scientific names The other six were mislabeled with an important mislabeling from P hypophthalmus into P boucourti Although no commercial frauds were found in these mislabelled products, the correct scientific names of fish species should be labelled for the processed products as they are in supermarkets Thuy Thi Vo Bich, Hieu Duc Nguyen, Thu Thi Hoai Nguyen, Ha Thi Thuy Tran Tra catfish (Pangasianodon hypophthalmus) is a commercially important aquaculture species in the Mekong Delta, Vietnam; accounting for approximately 60% of Vietnam freshwater fish production However, the food trade globalization and the growing demand for selected varieties of food have led to the intensification of commercial fraud, especially in the form of substitution species and mixing with cheaper taxa DNA bar-coding methods have shown a potential well proven molecular approach to assess the authenticity of food items In this study, we utilized high throughput NextSeq500 sequencing to capture and identified SNP markers on 400 wild and farming catfish individuals from Vietnamese provinces (e.g., An Giang, Dong Thap, Can Tho, Tien Giang, Ben Tre, Hau Giang, Vinh Long, and Ho Chi Minh City) and 10 international locations of Cambodia, Thailand and Turkish Journal of Fisheries and Aquatic Sciences, 18: 457462 (2018) MOJ Current Research & Reviews, Vol (1), 2018 Số TT Nội dung Đơn vị chủ trì, Tác giả Tóm tắt nội dung Ghi Bangladesh Stringent filtering of SNP-calling parameters resulted in 11,009 SNP loci represented across all countries We utilized the VCF tools to validate two panels of SNPs selected from the NGS data Selection criteria included SNPs shared between catfish’s Vietnam and international populations, and SNPs specific to each population A total of 780 and 809 SNPs were filtered in Vietnamese and international catfishes, respectively, the final 12 special SNPs were detected SNP validation by using Sanger sequencing and PCR showed a result of per 12 SNPs was confirmed successfully in 300 individuals from Vietnamese catfishes The first times our results provide genotype informative marker loci in Vietnamese Tra catfish in order to approach molecular traceability in aquaculture species with minimal previous genetic information Genetic relationship of asiatic hard clam populations collected in northern coastal provinces in Vietnam based on mtDNA sequence analysis Vu Thi Trang, Le Thi Quynh Chi, Chu Chi Thiet, Nguyen Huu Duc, Tran Thi Thuy Ha The genetic relationship of some Asiatic hard clam (Meretrix meretrix) based on mtDNA COI sequence analysis was investigated for populations collected in Thai Binh, Nam Dinh, Nghe An provinces in Vietnam In addition, this research also targets at species identification based on COI sequences In total of 59 sequences analyzed, 19 sequences belonged to Meretrix meretrix species with Gen Bank accession number DQ399399.1 17 sequences of M meretrix were used for genetic relationship analysis among populations In which, polymorphic sites, parsimony informative sites and haplotypes observed for the COI gene Moderately genetic population diversity was observed, overall haplotype and nucleotide diversity were 0.476±0.233 and 0.00151±0.00069, respectively Generally, genetic differentiation (FST) (FST < 0.15) was moderate The genetic distance was rather low, which ranged from 0.001 (Thai Binh–NgheAn, Thai Binh–Nam Dinh populations) to 0.002 (Nam Dinh – Nghe An populations) The result of haplotype network constructing indicated that populations shared common haplotype and there was no specific isolation of the haplotypes of the populations Hence, it showed M meretrix populations had intimate genetic relationship The result of phylogenic tree indicated that three M meretrix populations (Thai Binh, Nam Dinh, Nghe An) had a very small or no genetic variation among populations Journal of Aquacultu re & Marine Biology, Volume Issue 2018 Số TT Nội dung Đơn vị chủ trì, Tác giả Tóm tắt nội dung Ghi Modelling Tools to Analyze and Assess the Ecological Impact of Hydropower Dams Thi Hanh Tien Nguyen, Gert Everaert, Pieter Boets, Marie Anne Eurie Forio, Elina Bennetsen, Martin Volk, Thu Huong Thi Hoang and Peter L M Goethals We critically analyzed a set of ecological models that are used to assess the impact of hydropower dams on water quality and habitat suitability for biological communities After a literature search, we developed an integrated conceptual model that illustrates the linkages between the main input variables, model approaches, the output variables and biotic-abiotic interactions in the ecosystems related to hydropower dams We found that variations in water flow and water depth coupled with increased nutrient availability are major variables that contribute to structural and functional ecosystem changes We also found that ecological models are an important tool to assess the impact of hydropower dams For instance, model simulation of different scenarios (e.g., with and without the dam, different operation methods) can analyze and predict the related ecosystem shifts However, one of the remaining shortcomings of these models is the limited capacity to separate dam-related impacts from other anthropogenic influences (e.g., agriculture, urbanization) Moreover, collecting sufficient high-quality data to increase the statistical power remains a challenge The severely altered conditions (e.g., generation of very deep lakes) also lead to difficulties for standardized data collection We see future opportunities in the integration of models to improve the understanding of the different processes affected by hydropower dam development and operation, as well as the use of remote sensing methods for data collection Water 2018, 10, 259 Threshold Responses of Macroinvertebrate Communities to Stream Velocity in Relation to Hydropower Dam: A Case Study from The Guayas River Basin (Ecuador) Thi Hanh Tien Nguyen 1,2, Marie Anne Eurie Forio 1,*, Pieter Boets 1,3 , Koen Lock 1, Minar Naomi Damanik Ambarita 1, Natalija Suhareva 1,4, Gert The Guayas River basin is one of the most important water resources in Ecuador, but the expansion of human activities has led to a degraded water quality The purpose of this study was (1) to explore the importance of physical-chemical variables in structuring the macroinvertebrate communities and (2) to determine if the thresholds in stream velocity related to macroinvertebrate community composition could be identified in the Guayas River basin Thus, macroinvertebrates and physical–chemical water quality variables were sampled at 120 locations during the dry season of 2013 in the Guayas River basin Canonical correspondence analysis (CCA) was performed to identify relevant physical–chemical characteristics of the river influencing the Water 2018, 10, 1195; doi:10.339 0/w10091 195; Page 2-17 Số TT Nội dung Đơn vị chủ trì, Tác giả Everaert , Christine Van der heyden 5, Luis Elvin DominguezGranda 6, Thu Huong Thi Hoang 7; and Peter Goethals Tóm tắt nội dung distribution of the macroinvertebrate communities Threshold indicator taxa analysis (TITAN) was used to discriminate between the macroinvertebrate community related to stagnant waters (Daule–Peripa reservoir) and to running waters CCA indicates that the most important environmental factors influencing the distribution of macroinvertebrate communities were stream velocity, chlorophyll concentration, conductivity, temperature and elevation Tipping points for the macroinvertebrate community were defined by stream velocity at 0.03 m/s and 0.4 m/s, i.e., stagnantwater (including dam-related reservoirs)taxastarttoquicklydecreaseinabundanceandfrequencyat0.03m/swhilerunnin g-water taxastarttoquicklyincreaseinabundanceandfrequencyat0.03m/suntilastreamvelocityof0 4m/s The results provide essential information to define environmental flows to further support water management plans of the Guayas River basin Information obtained wil To isolate and characterise free nitrogen-fixing bacteria, we collected randomly soil samples from different areas of Ha Noi Nitrogen-fixing bacteria were isolated using Burk’s medium without nitrogen mineral supplement The ammonia (NH4+ ) synthesis Characterization and identification of nitrogen-fixing bacteria isolated from agricultural soil Tran Thi Thuy Ha, Thai Thi Lam, Nguyen Thanh Huyen, Nguyen Xuan Canh Ghi of these bacterial strains after biomass production was determined by means of Nessler reagent Based on the results of isolation, we observed and evaluated colony and cellular morphology, pigment production, and metabolic activities of twenty-five isolates Among the isolated bacteria, two bacterial strains (6.2 and 8.2) with high NH4+ concentration in the cultural medium were selected as the best strains for nitrogenfixing ability The optimal pH and temperature for their growth and nitrogen fixation are 7.0 and 30°C, respectively Growth is best favored in the presence of sucrose We sequenced the 16S rRNA gene of selected strains and compared the homology of them in GenBank using BLAST search The result of the comparison shows that the 6.2 and 8.2 strains have 99% and 100% 16S Vietnam Journal of Science, technolog y and engineerin g Vo; 60, No Sep, 2018 Page 4854 Số TT Nội dung Đơn vị chủ trì, Tác giả Tóm tắt nội dung Ghi rRNA-sequence similarity with Pseudomonas sp and Bacillus sp., respectively Effects of Enriching Nitrogen and Phosphorus on the Growth of Sargassum Podacanthum Cultured in Potassium-Fortified Inland Saline Water Effects of temperature and pH on the growth of Sargassum linearifolium and S podacanthum in Ha Thi Thu Bui, Trong Quoc Luu and Ravi Fotedar Ha Thi Thu Bui, Trong Quoc Luu and Ravi Fotedar Potassium-fortified inland saline water (K+ISW) has shown potential for growing marine species, including seaweed species The response of a brown seaweed species, Sargassum podacanthum, to nitrogen and phosphorus enrichments were evaluated by culturing the species for 84 days in K+ISW and comparing it with Ocean Water (OW) The culture media were enriched weekly with ammonium chloride and sodium dihydrogen phosphate, with ammonium and phosphate ratios of 10:1 at five different concentrations 80:8, 120:12, 160:16, 200:20 and 240:24 ẻẳM The culture medium with no enrichment was used as a control The water quality and biomass of S podacanthum were measured fortnightly The S podacanthum biomass increase significantly with different concentrations of the nutrient supplementations The standing biomass and Specific Growth Rate (SGR) of S podacanthum were similar in OW and K+ISW in the absence of any nutrient supplementation and at the supplement concentration of ammonium and phosphate 160:16 ẻẳM However, from day 42 onwards, at the ratios of 80:8, 120:12, 200:20 and 240:24, S podacanthum cultured in OW grew significantly faster than in K+ISW In K+ISW, optimal growth of S podacanthum was observed at the 160:16 and the increase in biomass was significantly higher than the initial biomass until day 70, whereas at the other four nutrient supplement concentrations, the S podacanthum biomass remained unchanged during the entire culture period The nitrite, total Kjeldahl nitrogen and phosphate concentrations in water were found to be significantly (p