Khảo sát tác dụng hạ đường huyết của một số loại thảo dược trên mô hình chuột in vivo

TAP CHI KHOA HOC TRIJONG DAI HOC Ma TP.HCM - SO (37) 2014 11 KHAO SAT TAC DUNG HA DUONG HUYET CUA MOT SO LOAI THAO DON MEN MO HiNH CHUOT IN VIVO Ngiy nhin bii: 19/04/2014 Ngay nhin lai: 19/06/2014 Ngay duylet ding: 07/07/2014 Ho Thi Huyen Trang, Phan: Thi Ng9c Bich, Pham Xuan Xinh, Trwong Thi B9ch Van,' Vu Tien Luyen2 Lao Dem Thuan3 TOM TAT Dai thcio &tang la chirng benh thuitng gap tren the giol veri bleu hien la su tang cao ye &rang huyet bat thuang tac clang caa insullin Hien nay, viec sir clung cac loaf thcio chew cac bai thuoc dan gian clang dugs quan tam cluing mang nhieu lai ich Trong nghien caw nay, chang tOi tien hanh khcio sat Hui nang dyeing huyet caa cao chiet trai dau bap, hat methi, re hoang lien va tra Kombucha tren mo hinh chuat dai thao clueing Ket qua cho thay cao chief- trai dau bap (40 g/kg), hat methi (30 g/kg) va re cay hoang lien (150 mg/kg) c6 cho tac clung manh tac ,dting clueing huyet Trong do, cao chiet dau bap va cciy hoang nhcit gitip &tang huyet chuat tic 500 mg/dl xuang du& 200 mg/dl Ngoai ra, cac chi tieu sinh lv chuat thi nghiem deu a mar on dinh Tir do, cluing toi ket luan cao chiet trai dau bap, hat methi va re cciy hoang lien co tac dung hg clueing huyet a nhiing lieu duos khcio sat va an toan vai chuOt thi nghiem Tir khoa: Benh dai thao &row, mo hinh chuOt dai thao throng, dau bip, hat methi, Hoeing Lien ABSTRACT Diabetes mellitus is a common worldwide disease with hyperglycaemia due to abnormal activities of insullin The use of traditional medicine in treatment of diabetes has gained more attention recently because of its convenience In this study, the hypoglycaemic effects of the ethanol extracts from water okra fruit, methi seeds, Coptis teeta Wall (hoang lien) roots and Kombucha tea are carried out on diabetic mouse model induced by alloxan Results show that water okra fruits (at 40 g/kg), methi seeds (at 30 g/kg) and hoang lien roots (at 150 mg/kg) posess hypoglycaemia effect Especially, water okra fruits and hoang lien roots reduce blood glucose from the high level (> 500 mg/dl) to normal level (< 200 mg/dl) without affecting mouse normal physiology Thus, we conclude that the extracts of water okra fruits, methi seeds and hoang lien roots have the anti-hyperglycaemic effects in diabetes Keywords: Diabetes, diabetic mouse models, water okra, methi seeds, Coptis teeta Wall TruCrag Dgi hpc Ma TP.HCM Trufmg Dgi hoc Khoa hpc Tip 1Vhien TP.HCM, Dgi hpc Quoc Gia TP.HCM ThS, Tneang Doi hoc Ma TP.HCM Email: ducthuan.ld@ou.edu.vn I CONG NGHE 12 flat van de Dai thao, dueing, (Diabetes mellitus) la chirng benh ve chuyen hoa clueing huyet181 Bleu hien lam sang, ciia benh la six tang cao ming de clueing huyet str thieu but insullin (type I) hoac sai hong hoat (king ciia insullin (type II) gay anh hoang den cac qua trinh chuyen hoa carbonhydrate, chat beo va protein1121 Theo thong ke vao nam 2013, Viet Nam c6 khoang 3,3 trieu ngtroi benh chiem ti le 5,37% dan se, la met nam mac c6 so ca mac benh cao nhat a khu vtrc tay Thai Binh Duane' Dieu clang ghi nhan rang ty le mac benh dai thao duang tren the gioi cang gia tang clang ke, theo dtr doan vao nam 2025 so ca mac benh tang den khoang 52.7 trieu ngueri (5.4% dan so the gieri)181 Ve dieu tri, hien nay, ben canh cac loai thuoc thuang mai tren thi trtremg, benh nhan c6,the dieu tri bang cac phtrang phap y hoc co truyen vi cac phtrang phap it ton kern, it gay tac dung phu der (lilac sir dung lau dai dan gian va der dugc xay dung hoan chinh bai ban ket hop gifia viec sir dung thao dugc va cac phtrang phap chira tri be trai l 11 Chinh vi very, cac loai thao dugc tir nhien co tac dung dieu tri hay hO trg dieu tri benh dai thao clueing dang dugc tap trung nghien dru va phat trien nham thay the cac thuoc tong hop hoa hoc dang dugc sir dung hien nay1111 Trong dan gian, met s6 loai did° dugc dugc cho la c6 ding dung hi; trg dieu tri dai thao duang chang han nhtr dau bap (Abelmoschus esculentus), re cay hoang lien (C'optis teeta Wall), hat methi (Trigonella L.), tra foenum-graecum 0103104105i Tuy nhien, hien Kombucha nhang nghien cfru de cap den kha nang duang huyet cua cac loai thao dugc tren O Viet Nam van han che Xuat phat tir thuc to tren va dua tren nhanp ket qua ciia Lao Dire Thuan va cs (2013)131, Chung ten tien hanh nghien cuu khdo sat kha nang 110 trg clueing huyet cua cac chiet xuat cao fir trai dau bap hat methi, re cay hoang lien va tra Kombucha tren mo hinh chuet bach (Mos musculus var Albino) bi benh dai thao duang bai tac nhan hoa chat Alloxan (A7413, Sigma) itham tao tier) de cho viec nghien dru Oat trien nhOng bai thuoc dan gian co tac dung 113 trg dieu tri duang huyet Phulyng phi!) nghien cuu 2.1 Sv luvc mot so Mao doge dwvc set dung nghien au Dau bap (Albelmoschus esculentus) la loai cay than thao hang nien, thuerng dugc a khap Viet Nam., Qua dau bap co tac dung On dinh clueing huyet mau, giam xa vita deng mach ngan chan skr di can cna ung thu dai true trang va met se tac dung khac131 Cay methi (Trigonella Ibenum:graecurn L.) la loai cay than thao hang nien, nhieu cac nuo.c Nam A va viing Dia Trung Hai Hat methi dugc sir dung nhu on loai thuc pham chirc nang co ,tac dung On dinh clueing huyet, giam cac roi loan tieu hoa, giam cholesterol va met so tac dung khacl" Cay hoang lien (C'optis teeta Wall) la loaf thaodtrac mac tren day Floang Lien mot San Phan than va re cua cay c6 tac dung khang khuan, khang virus va On dinh duang huyet ding met so tac dung khacI31 Tra Kombucha la loai tra len men tir he vi khuan Acetobacter va met so loai nam men thuec chi Zygosacchromyces Brettanomyces Tra Kombucha co tac dung tang cueing sirc de khang, phong ngira va dieu tri tieu duang, cai thien chirc nang gan, roi loan tieu h6a va cac tac dung dirge 1/ khacl" 2.2 Thu nhiin cao chiit ethanol trai dim bap, kit methi va re cay hang lienI31 Dan bap, hat methi va re cay hoang lien dtrgc phai kha, sau cac loai dugc lieu dugc xay nho va ngain vai ethanol 850 theo ty le 1:1 Sau ngay, dich chiet dugc co quay duoi dung mai cho den tao han hgp set 2.3 Thu 'ikon dung dich tra Kombucha Ntrac cat dun soi vai duang theo ty le 10:1 va b6 sung cac tra tai lac co san tren thi truOng Sau do, dich tra dugc b6 sung them men (0,3% w/v) va dich tra Kombucha c6 san (0,3% v/v) Tra dugc u a nhiet de phong tir den 15 Tra sau u co pH = 2,5-3,0 13 TAP CHI KHOA HOC TRUaNG DAI HOC MO TP.HCM - S6 (37) 2014 2.4 D'Ong vitt thi nghiem ChuOt bach (Mus musculus var Albino) c6 ltrang trung binh 22,5 ± 2,5 g dtrac mua tir vien Pasteur Thanh ph6 H6 Chi Minh va nu6i duang tai phong thi nghiem sinh ly d6ng vat, Twang Dai hoc Ma TP.HCM vai chu kSt sang tai 12/12 ChuOt ducc nuoi on dinh truck thi nghiem 2.5 T90 mo hinh chit& tiau bang Alloxani31 ChuOt &pc chia hai 16 thi nghiem Lo tiem Alloxan (200, mg/kg) va 10 tiem mak cat Sau gi6, tien hanh tiem 0,5 mL dung dich glucose 5% o ca hai 16., Sau 72 gio., chu6t dtrac kiem ,tra dithng huyet Chu6t co gia tri duang huyet tren 200 mg/dl duac xem nhu cong va sir dung cho nhi-mg thi nghiem khao sat kha nang 116 try &rang huyet sau 2.6 Kith° sat Mc dung h9 throng huyb can cac loci thdo &rot Chu6t sir dung thi nghiem deu tang thai benh dai thao twang voi chi so throng huyet 16n han 200 mg/dl Chu6t ducyc chia 6,10, m sai 16 Lo doi chirng am cho chu6t Ong ntrac cat L6 doi chi:mg duang cho chu6t uong Gliclazide (Stada) co tac dung duang huyet cac 16 thi nghiem 1, 2, va 4, cac chu6t ducyc tien hanh cho uong theo thin to nhtr sau: cao chiet tau bap (40 g/kg), cao chiet hat methi (30 g/kg), cao chiet re cay hoang lien (150 mg/kg) va tra Kombucha (0.09 ml/con) moi uong hai linI31141161191 Cac chi so ye throng huyet, luting sinh ly mau dtrac kiem tra khoang thai gian 20 thi nghiem 2.7 Hwang phdp xer ly thong ki S6 lieu va ket qua thu nhan dtrac phan tich va thong ke bang cong cu ANOVA phan mem Microsoft Excel 2007 va phan mem Statgraphic 3.0 Ket qua duac trinh bay du.61 Bang MEAN ± SEMI21 Ket qui va thio 1u4n 3.1 Ket quii too mo hinh chu0 lieu &ton being Alloxan Bing Su thay dai ding duirng huyet Marc va sau tiem Alloxan 72 giir Chi so duirng huyet &trot tiem Alloxan (mg/di) Chi so duirng huyet sau tiem Alloxan (mg/di) Ty 1e cong (%) La (200 mg/kg) 134,0 ± 6,1* 368,3 ± 21,7** 70 % La (nircrc cat) 133,4 ± 7,4a 135,8 ± 6,5a 0% Ghi chit: Khac biet coy nghTa thong kip voi tin ca'y 95 % ChuOt sau tiem Alloxan lieu 200 mg/dl c6 sir tang len rO ret ve chi so twang huyet sau 72 gio thi nghiem ti le ding la 70%, 16, chimg am khong thay co sir tang len ye chi so dui:mg huyet Ket qua tren phii hop yen nghien ciru truck ctla Lao Dirc Thuan va cs (2013) xay dung mo hinh chuOt tieu duang bang Alloxan131 Ngoai ra, phuang phap cling cho ket qua tuang by yeti cac phirang phap gay dai thao duang chu6t cac nh6m nghien ciru khac tren the Nhu vay, c6 the khing dinh ring chiing tai da xay dung ding m6 hinh chu6t tieu dtrOng va co the sir dung cho cac nghien ciru tiep theo 14 GONG NGHE 3.2 Dui niing throng huyit ciia cao chiet trai dnu biip, hat methi, re cay holing lien va dung dich Ira Kombucha Bang Su' thay &di tiling di) throng huyet (mg/di) thoti gian Maio sat NO N5 N1O N15 N20 532.85 ± 2.56 528,86 ± 4,36 527,00 ± 6,33 524,00 ± 3.25 527,29 ± 4,20 Le dOi ching throng 531,29 ± 4,22 470,71 ± 7,53 362,57 ± 7,06 239,43 ± 6,49 173,57 ± 4.83 Lo dOi chting am Le (Dau bap) 528,57 ± 5,14 418,49 ± 14,16 328,80 ± 22,34 241,92 ± 16,37 186.75 ± 6,28 Le (Hat methi) 526,43 ± 6,83 434,43 ± 7,94 334,29 ± 10,98 281,71 ± 15,61 256,57 ± 18,67 Lo (Re lining lien) 519,57 ± 22,05 480,86 ± 25,57 486,29 ± 22,58 381,14 ± 13,38 172,00 ± 16,08 515,43 ± 2,74 Lo (Kombucha) 511,57 ± 1,77 505,43 ± 7,61 497,14 ± 7,91 499,00 ± 9,04 Hinh DO thi bieu (lien sty bien thien tiling di) dtrirng huyet cna chuOt thi nghien 600 AM -a- De IN row; -to- PAU BAP 300 -110ANO LIEN K( NO N5 NIO Nt.:AV DO thi a Hinhl cho thay, lo chirng am (chuiit duct cho uong ntroc cat) khong co su thay doi ve nOng (10 duang huyet Oren 500 mg/dl) ching to chuOt van a trang thai benhl dai thao throng Cac lo thirighiern 1, va co tac dung throng huyet sau 20 thi nghiem.Trong do, cao chiet dAu bap va re hoang lien có tac dOng tuang to lo doi chang ducmg ye kha rang duang huyet chuOt (chirng throng: 173,57 mg/dl, dau bap: 186,75 mg/di, hoang lien: 172,00 mg/dl) va dua chi so duong huyet mire binh throng (106-278 mg/d1)1 lo 3, cao chiet methi co kha nang duemg huyet cua chuOt (256,57 mg/di) nhung chin duce &rang huy8t cluai mire 200 mg/d1 Tuy nhien, ket qua dm tra NIS N20 NGI-11101 Kombucha (164) kh6ng có tac dung &rang huyet va có nong dO dtrang huyet gan ducmg vai 10 chirng am (ching am: 527,29 mg/di, tra Kombucha: 499.00 mg/dl) Cac ket qua a 10 1, 2, cho thiy co sir thong &Ong vai cac nghien cfru truck ye tac dOng throng huyet cua trai , dau bap (Tra (Trap Hoang Dung, HuS/nh Xuan Yen (2012)), dm hat methi (Nawel Hamza VA cs (2012)) va ctia re cay hoang lien (Lao Dire Thuan va cs (2013))1I][311101 Tuy nhien, ngupc lai nghien cfru cua Thummala Srihari va cs (2013), tac dOng cua tra Kombucha tren mo hinh chuOt tier throng cua chung toi khong cho ket qua dtremg huyet1141 15 TAP CHI KHOA HOC TRUONG DAI HOC MO TP.HCM - So (37) 2014 Nhu vay, viec sir dung cao chiet cua hat dau bap, hat methi va re cay hoang lien deu có tac dung duiyng huyet tren mo hinh cua chung toi Tuy nhien, tra Kombucha khong co tac dung De kiem tra tic deng cua cac loai cao chiet va tra Kombucha tren chuet, kiern tra cacs chi so ve Chung toi tien tong hugng hong cau va tng luting,, tong lu ng bach cau ti 3.3 Chi lieu troll.? Item Bang Trong lucnig cua chuiit (g) qua cac thi nghiem o cac to kink NO N5 N1O N15 N20 L8 del ching am 19,36 ± 0,65 22,36 ± 0,83 22.90 ± 0,50 23,06 ± 1,47 24,13 ± 1,10 LO deli chting throng 20,41 ± 0,77 21,27 ± 0,81 22,42 ± 0,58 23,90 ± 1,49 24,44 ± 1,10 Lo (Nu bip) 20,22 ± 0,32 20,93 ± 0,27 21,89 ± 0,46 22,10 ± 0.49 22,94 ± 0,46 Lo (Hat methi) 19,39 ± 0,31 21,21 ± 0,55 21,85 ± 0,59 22,98 ± 0,76 24,95 ±1,39 L8 (Re holing lien) 20,41 ± 0,23 21,46 ± 0,28 22,45 ± 0,28 23,39 ± 0,23 24,19 ± 0,23 L8 (Kombucha) 20,16 ± 0,22 21,58 ± 0,19 23,01 ± 0,17 23,98 ± 0.13 24,82 ± 0,01 Hinh DO thi bieu dien sty bien thien luting cua at, eit thi nghii2m 30 25 NO N5 NGAI" NIO NI5 N20 NGIIIEN1 Trong luting cua chuet la , met nhang chi tieu dau tien can xet den danh gia hieu qua tac deng cua vat lieu dtrov thi nghiem tren chuet Trong luting trung binh cua chuet dua vao thi nghiem nam khoang 20 gram tai Sau 20 ngdy khdo sat, luting trung binh cua chuet tang Ian loot la 24,13; 24,44; 22,94; 24,95; 24,19; 24,82 g tucmg irng ran loot Or cac 10 dei chUng am, doi chUng &rang, 10 sir dung cao chiet trai au bap, cao chiet hat methi, cao chiet re hoang lien va tra Kombucha Trong luting cua chuet co six tang len nhung khong CO khac biet ye mat thong ke so vai Ngoai ra, luting cua chuet a cac 10 khac ding khong co su khac biet ve mat thong ke Dieu chimg to viec sir dung cao chiet cac foal thao dugc va tra Kombucha khong gay anh huong den luting cua chuet thi nghiem 16 CONG NGHE 3.4 Chi tieu hOng ) aim Bang Chi so hOng cau (x 10' bao/mm) ciia chuOt qua cac thi nghiem NO N5 N1O N15 N20 1054,30 ± 16,75 1069,90 ± 14,76 1057,40 ± 10,16 1087,90 ± 16,67 1097,7 ± 18,60 Lo diii chirng dtrcrng 1078,40 ± 125,79 936,36 ± 24,24 837.86 ± 50,18 828,71 ± 41,54 746,14 ± 42,53 Lo (Nu big) 1008,14 ± 30,48 828,00 ± 13,69 835,49 ± 11,72 852,70 15,25 849,51 = 20,91 L8 (Hat methi) 1096,70 ± 12,01 959,57 ± 8,577 851,86 ± 12,42 803,86 ± 12,94 770,71 ± 12,57 Lo (Ile hoang lien) 1049,60 ± 13,84 952,00 ± 15,03 870,42 ± 12,75 806,57 ± 5,576 783,71 ± 4,098 1071,30 ± 13,77 1064,60 ± 14,31 1092,10 ± 13,24 1101,80 ± 21,14 1099,80 ± 27,35 Lo diii chtrng am Lo (Kombucha) Hinh DO thi bieu (lien sy bien thien chi so hong cau ciia chuet thi nghiem 1200 1000 800 DC AM -III- DC DI:ONG GOO DAL' BAP x 400 'IETHI -HOANG LIEN 2tX) ?, -KOMBUCHA NO N5 N10 N15 'GAY T111 NCI-1101 Ket qua cho thay co six lien he gifra dai than duang va tong lugng hong cau,cim chuOt sir tang len ve duemg huyet dan den six glycate him hemoglobin gay thieu but hemoglobin On yeti oxy Hien tucmg can den six tang len to bao hong cau de bo sung cho lacing hemoglobin bi glycate hoa Vao 0, lugng hong cau tat ca cac lo thi nghiem deu vuert ngu6ng gia tri gieri han binh thu6ng cua chuOt la - 9,5 x 106 t6 baohnm3171 Sau 20 thi nghiem, chi so hiing au lo dOi chirng khong co six thays doi lo chimg duang c6 tong lucmg hOng cau giam ro ret va nam ngifang sinh ly binh thlrerng cua N20 chuOt (7,5 x 106 to bao/mm3) Cac lo 1, 2, CO tac dung tong so hong cau tuong to lo doi chirng clucrng va deu nam gi6i han sinh 1j, binh thireing lo so khorig có tic Flung Ket qua phii ,hop vai ket qua ye kha nang clueing huyet cua cac loai thao duo.c (mac 3.2) va nghien cau dm Lao Dirc Thua'n va cs (2013)131 Dieu cho thay rang sau sir dung cac loai cao chiet, chi so hong cau cua chuOt da dugc on dinh va ram gi6i han sinh lY binh thuerng TAP CHI KHOA HOC TRUONG DAI HOC MO TP.HCM - SO (37) 2014 17 3.5 Chi tieu kick du Bing Chi se 4ch cau (te bao/mm3) cua chi* qua cac thi nghiem NO N5 N10 N15 N20 6082,3 ± 567,0 6585,3 ± 666,5 7468,7 ± 2448,4 7590,1 ± 940,7 8235,0 ± 1411,0 Lo TM cluing throng 6127,1 ± 474,6 7302,6 ± 780,8 7943,7 ± 2003,6 8258,6 ± 630,9 8403,9 ± 634,3 LO (Nu bip) 6151,0 ± 386,7 6437,1 ± 231,4 7238,6 ± 357,2 7932,7 ± 273,9 10434,3 ± 312,5 Le (Hat methi) 6069,7 ± 31,5 6567,4 ± 51,3 7767,4 ± 54,8 8701,7 ± 81,1 9532,1 ± 315,4 Lo (Re hoing lien) 6157,6 ± 77,5 7515,3 ± 45,5 7729,4 ± 47,5 7924,9 ± 140,1 8398,3 ± 52,4 Lo (Kombucha) 6065,4 ± 7,3 6066,0 ± 12,7 6739,8 ± 176,6 6958,0 ± 144,0 7204,1 ± 20,5 Le dOi chting am Hinh DO thi bieu dien str bien thien tong se bach cau cua chuet thi nghiem 11000 10000 8000 • DC AM -III- • • 6000 ()t DAC BAP 4000 -NIETHI • -HOANG 1.111: \ 2000 KONIBUCIIA NO N5 N10 N15 N20 NGAY NG13101 Vao 0, tong luting bach cau cua cac 16 thi nghi8m deu nam khoang sinh 1j/ binh thtrimg171 Sau 20 ngay, Kiang bach cau cac 16 thi nghi8m c6 tang len nhung van nam khoang gia tri sinh ly binh thuang (314,2 x 103 to bao/mm3) Su tang bach cau a day c6 th8 nhi6'm qua trinh tiem,va gia tri throng huyet Ket qua cho thay cac dang cao chiet thao dtrac va tra Kombucha khong gay anh huang len 118 mien dich cua chu6t thi nghi8m Dua tren cac k8t qua thu nhan dtro.c, thong t6i nhan thay cao chi8t trai dau bap lieu 40 g/kg, cao chiet hat methi lieu 30 g/kg va cao chi8t r8 hoang lien lieu 150 mg/kg c6 kha nang &rang huyet a mo hinh chu6t thi nghi8m Ngoai ra, cac did° clugc sir dung nghien ciru khong anh huang den cac chi deli sinh ly binh thtrang cua chu6t Ket 1u4n Tir cac ke't qua thi nghi8m dat dtrac, cao chiet trai au bap, hat methi, cay hoang lien thir nghi8m tren mo hinh d6ng vat co tac dung throng huyet sau 20 sir dung a cac li8u 40 g/kg, 30 g/kg va 150 mg/kg Tuy nhien, tra Kombucha khong c6, tac dung dui:mg huyet a lieu 0,09 ml Tat ca cac loaf thao &rot sir dung nghien ciru a cac lieu tren la an toan dOi vai chu6t thi ngshi8m the hi8n qua luting, chi so hong cau va chi so bach cau 18 CONG NGHC TAI LIEU THAM KHAO Dang Van Giap 1997, Phan tich XI lieu khoa hoc bang charting trinh ES-Excel, NXB Giao due, tr.45-46 Dufresne C., Farnworth E 2000, 'Tea, Kombucha, and health: a review' Food Research International, 33, p.409-421 Hamza N., Berke B., Cheze C., Le Garrec R., Umar A., Agli AN., Lassalle R., Jove J., Gin H., Moore N 2012, 'Preventive and curative effect of Trigonella foenum-graecum L seeds in C57BL/6J models of type diabetes induced by high-fat diet', Journal of Ethnopharmacology, 142, p.516-522 International Diabetes Federation 2014, IDF Diabetes Atlas 6th edition, p.13-14 Lao Dire Thuan, Dam Thi Thanh Duang, Nguy& Thi Thanh Xuan, Le Thi Anh Thy, Nguyen Vu Thanh Tang, Ho Thi Huyen Trang, Pham Hong Phi Long, Trinh Him Phuac 2013, 'Xay dkmg mo hinh chuOt tieu duan va khao sat hieu qua throng huyet cua cay hoang lien (C'optis teeta wall) ten mo hinh clOng vat', Tap chi Khoa hoc Trtforg Doi hoc MO Tp.HCM, so 3(31), tr.116-123 Li WL., Zheng HC., Bukuru J., De Kimpe N 2004, 'Natural medicines used in the traditional Chinese medical system for therapy of diabetes mellitus', Journal of Ethnopharmacology 92(1) p.1-21 Mark A., Peggy D., Cory B 2000, The Laboratory Mouse, CRC Press, p.20-21 Mohammad MZ., Sedigheh K., Mahmoodreza M 2014, 'Diabetes and related remedies in medieval Persian medicine', Indian Journal of Endocrinol Metabology, 18(2), p.142-149 Nilesh J., Ruchi J., Vaibhav J., Surendra J 2012, 'A review on: Abelmoschus esculentus' Pharmacia, 1(3), p.84-89 10.Paige Passano 1995, 'The Many Uses of Methi', Nutt-ion, 91, p.31-34 11.Salim B 2005, 'Diabetes mellitus and its treatment', International Journal of Diabetes and Metabolism 13, p.111-134 12 Tag H., Kalita P., Dwivedi P., Das AK., Namsa ND 2012, 'Herbal medicines used in the treatment of diabetes mellitus in Arunachal Himalaya, northeast, India', Journal of Ethnopharmacology, 141, p.786-795 13.Thummala S., Krishnamoorthy K., Natarajan A., Uppala S 2013 'Antihyperglycaemic efficacy of kombucha in streptozotocin-induced rats', Journal of Functional Foods, p.1974-1802 14.Tran Hoang Dung, HuS/nh Xuan Yen 2012, 'Tac dung dung huyet cua dich chiet nuot clau bap', Tap chi Hog! long Khoa hoc, so 8, tr.84-88 15.Udoamaka FE., Jose MP 2014, 'The use of plants in the traditional management of diabetes in Nigeria: pharmacological and toxicological considerations', Journal of Ethnopharmacology, 14, p.S 0378-8741  ... de Dai thao, dueing, (Diabetes mellitus) la chirng benh ve chuyen hoa clueing huyet181 Bleu hien lam sang, ciia benh la six tang cao ming de clueing huyet str thieu but insullin (type I) hoac... the Nhu vay, c6 the khing dinh ring chiing tai da xay dung ding m6 hinh chu6t tieu dtrOng va co the sir dung cho cac nghien ciru tiep theo 14 GONG NGHE 3.2 Dui niing throng huyit ciia cao chiet... mem Statgraphic 3.0 Ket qua duac trinh bay du.61 Bang MEAN ± SEMI21 Ket qui va thio 1u4n 3.1 Ket quii too mo hinh chu0 lieu &ton being Alloxan Bing Su thay dai ding duirng huyet Marc va sau tiem