Nghiên cứu ứng dụng nanochitosan trong phòng trừ bệnh thán thư hại ớt sau thu hoạch

I H C QU H IH NGUY U NG NG D NG NANOCHITOSAN TRONG B I th c ph : 605402 LU TP H CS T SAU THU HO CH u ng ng d n khoa h c: TS Tr cv ch m nh cv ch m nh cv Lu cb ov t ih nH m: cv c aH ng ch m b o v lu n c a Ch t ch H ng Khoa qu c s a ch a (n p- - NHI M V LU H Nguy ng MSHV: 12110193 23/02/1985 Ngh An th c ph u ng : 605402 u ng d ng nanoch b t sau thu ho II NHI M V I DUNG: - T o ch ph m nanochitosan b Sodium tripolyphosphat (STTP) - nh ng tri n c a n m tri n c a n m - nh c a n nh h o gel ion gi t ng c a ch ph n s sinh tr u ki n in vitro nh h ng c a ch ph u ki n in vivo n s sinh tr hi u qu b o qu n t b a t sau thu ho ch : 20/06/2014 V i TS Tr i L IC c r t nhi u s c L TS Tr nh s c g ng c a b y il ic , tr c ti su c hi n lu l i nh h n ng nghi p, b n ng d n cho em o nh u ki n thu n t lu nh kh e, p nhi u may m Em xin ngh , m vui cu c s ng n Th ng d ch b o h c t th c hi i Con xin c , anh ch nh h c t p t tr ih su H K n t p th ngh th c ph m, Khoa K thu p th y n d t, ch d y cho em ki n th i c n thi c t p, th c hi cs c kh c nhi u ni m vui s nghi p tr ng ic il ic ngh th c ph tr c hi h th c ph c t p, sinh ho t t Th y nh c tr ng TP H c hi n Nguy ng ii T LU S d i thu c b o qu n rau qu ng s ng Vi ngu n g c t ch n s c kh e d ng m t ch ph m sinh h gi m thi u t n th t sau thu ho o gel ion v n cs d Colletotrichum gleoesporioides nanochitosan 0,03% ng x t c ch c ch 50% (EC50) s m n ym mc n khu n l u ki n in vitro, n ng n m; n ng PDA t i 0,04% u ki n in vivo, m Kh c p ch t -1,3- u iii ABSTRACT Using chemical pesticides in vegetable reservation made adversely affect to human health and the environment Finding and using a natural biological product, which is non-toxic to minimize the post-harvest lost, are being concerned In this study, nanochitosan was formed by gel ion method with sodium tripolyphosphate, used as an antifungal agent against Colletotrichum gleoesporioides causing anthracnose on red chilli In vitro, nanochitosan concentration of 0,03% inhibited over 50% germination of fungal spore; the concentration inhibiting growth by 50% of colony on PDA was 0,04% In vivo, nanochitosan coating at concentration of 0,4% had well effective in the preservation of red chilli Results in this study show that physiological and biochemical quality of red chilli were insignificant changed after 12 days stored at 25oC compared with red chilli before storage The antifungal stimulation ability of nanochitosan by increased such compounds as polyphenol, chitinase and -1,3-glucanase was studied iv L uc ab k t qu ngu t lu n lu b t k m t ngu ib tk c th c hi u o Vi c tham kh u tham kh lu Nguy ng v M CL C Trang M U p thi t c M u N n c u c ti n c 4.2 c ti n U 1.1 Gi i thi u v t m sinh v t h c c t c a t 1.1.3 M t s lo i t ph bi n n xu nh sau thu ho 1.2 T ng quan v gi i t m c a Colletotrichum gloeosporioides i t sau thu ho ch 10 1.2.1 1.2.2 1.2.3 S 1.3 Bi c c c a ch ng n m C Gloeosporioides 11 c 12 n 12 nh sau thu ho t 15 1.3.1 Bi a 15 1.3.2 Bi 15 18 18 nochitosan 19 25 vi 28 1.5 Nh c li 30 1.5.1 30 1.5.2 c 31 U 33 u 33 2.1.1 u 33 2.1.2 N m Colletotrichum gloeosporioides 33 2.1.3 Ch ph m chitosan t o nanochitosan 33 u 34 t m 34 a t 40 43 45 46 3.1 K t qu ionic 46 3.2 Kh i t c a nanochitosan u ki n in vitro 48 3.2.1 ng c gloeosporioides 3.2.2 n n n m Colletotrichum ng PDA 48 ng c n sinh kh i s i n m Colletotrichum gloeosporioides 51 3.2.3 Kh c ch c ns n ym mc a n m Colletotrichum gloeosporioides 54 3.3 Kh h i t c a nanochitosan u ki n in vivo 57 3.3.1 K t qu nh c a n m Colletotrichum gloeosporioides 57 XXI Number of Means Error Mean Square 0.00352 Critical Range 003168 003316 003406 003465 003506 Means with the same letter are not significantly different Duncan Grouping Mea N CT A 91.3020 B 69.1426 C 43.1419 3 D 25.2736 E 13.4625 F 0.0000 ng c nt l n ym mc - Ty le mam sau 6h nuoi cay The ANOVA Procedure Duncan's Multiple Range Test for tylenaymam NOTE: This test controls the Type I comparisonwise error rate, not the experimentwise error rate Alpha 0.05 Error Degrees of Freedom 12 Error Mean Square 0.003523 Number of Means Critical Range 003258 003410 003502 003563 003606 Means with the same letter are not significantly different Duncan Grouping Mean N CT A 86.5040 B 75.0040 C 39.0044 3 D 11.5029 E 0.0000 E E 0.0000 - Ty le mam sau 9h nuoi cay The ANOVA Procedure Duncan's Multiple Range Test for tylenaymam NOTE: This test controls the Type I comparisonwise error rate, not the experimentwise error rate Alpha 0.05 Error Degrees of Freedom 12 Error Mean Square 0.000173 Number of Means Critical Range 003198 003347 003438 003498 003539 Means with the same letter are not significantly different Duncan Grouping Mean N CT A 100.0000 XXII B C D E E E b ng c a nanochit 87.0042 42.5024 14.0040 0.0000 3 3 0.0000 tb o - Duong kinh vet benh sau 48h The ANOVA Procedure Duncan's Multiple Range Test for DKVB NOTE: This test controls the Type I comparisonwise error rate, not the experimentwise error rate Alpha 0.05 Error Degrees of Freedom 10 Error Mean Square 0.000173 Number of Means Critical Range 002048 002140 002194 002229 Means with the same letter are not significantly different Duncan Grouping Mean N CT A 0.27300 B 0.20333 C 0.00000 3 C C 0.00000 C C 0.00000 - Duong kinh vet benh sau 72h The ANOVA Procedure Duncan's Multiple Range Test for DKVB NOTE: This test controls the Type I comparisonwise error rate, not the experimentwise error rate Alpha 0.05 Error Degrees of Freedom 10 Error Mean Square 0.00114 Number of Means Critical Range 002739 002862 002935 002981 Means with the same letter are not significantly different Duncan Grouping Mean N CT A 0.963000 B 0.853000 C 0.453667 3 D 0.393000 E 0.000000 - Duong kinh vet benh sau 96h The ANOVA Procedure XXIII t Tests (LSD) for DKVB NOTE: This test controls the Type I comparisonwise error rate, not the experimentwise error rate Alpha 0.05 Error Degrees of Freedom 10 Error Mean Square 0.010327 Critical Value of t 2.22814 Least Significant Difference 0.0035 Means with the same letter are not significantly different t Grouping Mean N CT A 1.683667 B 1.473667 C 0.883000 3 D 0.593333 E 0.000000 - Xy ly AUDPC The ANOVA Procedure Duncan's Multiple Range Test for AUDPC NOTE: This test controls the Type I comparisonwise error rate, not the experimentwise error rate Alpha 0.05 Error Degrees of Freedom 10 Error Mean Square 0.00034 Number of Means Critical Range 03355 03505 03594 03651 Means with the same letter are not significantly different Duncan Grouping Mean N CT A 46.4833 B 40.4333 C 21.4300 3 D 16.5566 E 0.0000 ng c tb u ki n in vivo - Duong kinh vet benh sau 48h The ANOVA Procedure Duncan's Multiple Range Test for DKVB NOTE: This test controls the Type I comparisonwise error rate, not the experimentwise error rate Number of Means Alpha 0.05 Error Degrees of Freedom 10 Error Mean Square 0.002668 Critical Range 001819 001901 001949 001980 Means with the same letter are not significantly different XXIV Duncan Grouping A B C C C C C Mean 0.2130000 0.1720000 0.0000000 N 3 CT 0.0000000 0.0000000 - Duong kinh vet benh sau 72h The ANOVA Procedure Duncan's Multiple Range Test for DKVB NOTE: This test controls the Type I comparisonwise error rate, not the experimentwise error rate Alpha 0.05 Error Degrees of Freedom 10 Error Mean Square 0.000047 Number of Means Critical Range 003874 004048 004150 004216 Means with the same letter are not significantly different Duncan Grouping Mean N CT A 0.803000 B 0.462667 C 0.323000 3 D 0.234333 E 0.000000 - Duong kinh vet benh sau 96h The ANOVA Procedure Duncan's Multiple Range Test for HHKL NOTE: This test controls the Type I comparisonwise error rate, not the experimentwise error rate Alpha 0.05 Error Degrees of Freedom 10 Error Mean Square 0.00256 Number of Means Critical Range 005081 005310 005444 005530 Means with the same letter are not significantly different Duncan Grouping Mean N DC A 1.503667 B 0.984000 C 0.784000 3 D 0.514333 E 0.283333 - Duong kinh vet benh sau 120h The ANOVA Procedure Duncan's Multiple Range Test for HHKL NOTE: This test controls the Type I comparisonwise error rate, not the experimentwise error rate XXV Number of Means Alpha 0.05 Error Degrees of Freedom 10 Error Mean Square 0.024555 Critical Range 005037 005264 005397 005483 Means with the same letter are not significantly different Duncan Grouping Mean N DC A 1.783000 B 1.353000 C 1.064000 3 D 0.835000 E 0.463667 - Xu ly AUDPC The ANOVA Procedure Duncan's Multiple Range Test for HHKL NOTE: This test controls the Type I comparisonwise error rate, not the experimentwise error rate Alpha 0.05 Error Degrees of Freedom 10 Error Mean Square 0.00012 Number of Means Critical Range 01992 02081 02134 02168 Means with the same letter are not significantly different Duncan Grouping Mean N DC A 79.083667 B 52.840667 C 40.683000 3 D 27.723333 E 12.244333 5 ng c b o qu n t a, Hao h t kh ng - Hao hut khoi luong cung cong thuc DC The ANOVA Procedure Duncan's Multiple Range Test for HHKL NOTE: This test controls the Type I comparisonwise error rate, not the experimentwise error rate Alpha 0.05 Error Degrees of Freedom Error Mean Square 0.000985 Number of Means Critical Range 005682 005921 006055 Means with the same letter are not significantly different Duncan Grouping Mean N CT A 19.442333 B 12.553333 3 C 4.573900 D 2.123267 XXVI - Hao hut khoi luong cung cong thuc thi nghiem The ANOVA Procedure Duncan's Multiple Range Test for HHKL NOTE: This test controls the Type I comparisonwise error rate, not the experimentwise error rate Alpha 0.05 Error Degrees of Freedom Error Mean Square 0.000011 Number of Means Critical Range 006385 006654 006804 Means with the same letter are not significantly different Duncan Grouping Mean N DC A 10.645667 B 6.874333 3 C 3.243667 D 1.674000 - Hao hut khoi luong giua cong thuc sau The ANOVA Procedure Duncan's Multiple Range Test for HHKL NOTE: This test controls the Type I comparisonwise error rate, not the experimentwise error rate Alpha 0.05 Error Degrees of Freedom Error Mean Square 0.005182 Number of Means Critical Range 005475 Means with the same letter are not significantly different Duncan Grouping Mean N DC A 2.122667 B 1.673333 - Hao hut khoi luong giua cong thuc sau The ANOVA Procedure Duncan's Multiple Range Test for HHKL NOTE: This test controls the Type I comparisonwise error rate, not the experimentwise error rate Alpha 0.05 Error Degrees of Freedom Error Mean Square 0.000012 Number of Means Critical Range 007743 Means with the same letter are not significantly different Duncan Grouping Mean N DC A 4.573333 B 3.244000 - Hao hut khoi luong giua cong thuc sau The ANOVA Procedure Duncan's Multiple Range Test for HHKL XXVII NOTE: This test controls the Type I comparisonwise error rate, not the experimentwise error rate Alpha 0.05 Error Degrees of Freedom Error Mean Square 0.000011 Number of Means Critical Range 007388 Means with the same letter are not significantly different Duncan Grouping Mean N DC A 12.553667 B 6.874100 - Hao hut khoi luong giua cong thuc sau 12 The ANOVA Procedure Duncan's Multiple Range Test for HHKL NOTE: This test controls the Type I comparisonwise error rate, not the experimentwise error rate Alpha 0.05 Error Degrees of Freedom Error Mean Square 0.000013 Number of Means Critical Range 007743 Means with the same letter are not significantly different Duncan Grouping Mean N DC A 19.443333 B 10.644000 c ng - Thay doi cung cung cong thuc DC The ANOVA Procedure Duncan's Multiple Range Test for DC NOTE: This test controls the Type I comparisonwise error rate, not the experimentwise error rate Alpha 0.05 Error Degrees of Freedom Error Mean Square 0.000018 Number of Means Critical Range 005516 005748 005878 Means with the same letter are not significantly different Duncan Grouping A B C D Mean 10.453667 10.233667 9.895333 6.743667 N 3 3 DC - Thay doi cung cung cong thuc thi nghiem The ANOVA Procedure Duncan's Multiple Range Test for DC NOTE: This test controls the Type I comparisonwise error rate, not the experimentwise error rate Alpha 0.05 Error Degrees of Freedom XXVIII Error Mean Square 0.024333 Number of Means Critical Range 003522 003671 003754 Means with the same letter are not significantly different Duncan Grouping Mean N DC A 13.162667 B 12.774000 C 11.162667 3 D 10.524667 - Thay doi cung giua hai cong thuc sau The ANOVA Procedure Duncan's Multiple Range Test for DC NOTE: This test controls the Type I comparisonwise error rate, not the experimentwise error rate Alpha 0.05 Error Degrees of Freedom Error Mean Square 0.103444 Number of Means Critical Range 005153 Means with the same letter are not significantly different Duncan Grouping Mean N DC A 13.164667 B 10.233000 - Thay doi cung giua hai cong thuc sau The ANOVA Procedure Duncan's Multiple Range Test for DC NOTE: This test controls the Type I comparisonwise error rate, not the experimentwise error rate Alpha 0.05 Error Degrees of Freedom Error Mean Square 0.124555 Number of Means Critical Range 004341 Means with the same letter are not significantly different Duncan Grouping Mean N DC A 12.773333 B 10.453000 - Thay doi cung giua hai cong thuc sau The ANOVA Procedure Duncan's Multiple Range Test for DC NOTE: This test controls the Type I comparisonwise error rate, not the experimentwise error rate Alpha 0.05 Error Degrees of Freedom Error Mean Square 0.023566 Number of Means Critical Range 005853 Means with the same letter are not significantly different Duncan Grouping Mean N DC A 11.163000 B 9.894333 XXIX - Thay doi cung giua hai cong thuc sau 12 The ANOVA Procedure Duncan's Multiple Range Test for DC NOTE: This test controls the Type I comparisonwise error rate, not the experimentwise error rate Alpha 0.05 Error Degrees of Freedom Error Mean Square 0.126555 Number of Means Critical Range 004719 Means with the same letter are not significantly different Duncan Grouping Mean N DC A 10.523333 B 6.742667 c - Thay doi mau sac cung cong thuc DC The ANOVA Procedure Duncan's Multiple Range Test for TDMS NOTE: This test controls the Type I comparisonwise error rate, not the experimentwise error rate Alpha 0.05 Error Degrees of Freedom Error Mean Square 0.533233 Number of Means Critical Range 003805 003965 004054 Means with the same letter are not significantly different Duncan Grouping Mean N DC A 36.353333 B 34.634667 C 32.114333 3 D 30.594333 - Thay doi mau sac cung cong thuc thi nghiem The ANOVA Procedure Duncan's Multiple Range Test for TDMS NOTE: This test controls the Type I comparisonwise error rate, not the experimentwise error rate Alpha 0.05 Error Degrees of Freedom Error Mean Square 0.51333 Number of Means Critical Range 004104 004276 004373 Means with the same letter are not significantly different Duncan Grouping Mean N DC A 41.964667 B 40.045000 C 39.452667 3 D 36.195667 i ho XXX - Thay doi hoat enzyme glucanase cung cong thuc E I The ANOVA Procedure Duncan's Multiple Range Test for GLU NOTE: This test controls the Type I comparisonwise error rate, not the experimentwise error rate Alpha 0.05 Error Degrees of Freedom Error Mean Square 0.000011 Number of Means Critical Range 002771 002888 002953 Means with the same letter are not significantly different Duncan Grouping Mean N DC A 0.334000 3 B 0.312333 C 0.273333 D 0.232000 - Thay doi hoat enzyme glucanase cung cong thuc E II The ANOVA Procedure Duncan's Multiple Range Test for GLU NOTE: This test controls the Type I comparisonwise error rate, not the experimentwise error rate Alpha 0.05 Error Degrees of Freedom Error Mean Square 0.000232 Number of Means Critical Range 003805 003965 004054 Means with the same letter are not significantly different Duncan Grouping Mean N DC A 0.392667 3 B 0.384000 C 0.325000 D 0.293000 - Thay doi hoat enzyme glucanase cung cong thuc E III The ANOVA Procedure Duncan's Multiple Range Test for GLU NOTE: This test controls the Type I comparisonwise error rate, not the experimentwise error rate Alpha 0.05 Error Degrees of Freedom Error Mean Square 0.000088 Number of Means Critical Range 004031 004201 004295 Means with the same letter are not significantly different Duncan Grouping Mean N DC A 0.553333 3 B 0.542667 C 0.434667 D 0.372333 - Thay doi hoat enzyme glucanase giua cac cong thuc khac sau XXXI The ANOVA Procedure Duncan's Multiple Range Test for GLU NOTE: This test controls the Type I comparisonwise error rate, not the experimentwise error rate Alpha 0.05 Error Degrees of Freedom Error Mean Square 0.000045 Number of Means Critical Range 004105 004255 Means with the same letter are not significantly different Duncan Grouping Mean N DC A 0.374667 3 B 0.292333 C 0.234000 - Thay doi hoat enzyme glucanase giua cac cong thuc khac sau The ANOVA Procedure Duncan's Multiple Range Test for GLU NOTE: This test controls the Type I comparisonwise error rate, not the experimentwise error rate Alpha 0.05 Error Degrees of Freedom Error Mean Square 0.000019 Number of Means Critical Range 004212 004365 Means with the same letter are not significantly different Duncan Grouping Mean N DC A 0.434000 3 B 0.385333 C 0.274000 - Thay doi hoat enzyme glucanase giua cac cong thuc khac sau The ANOVA Procedure Duncan's Multiple Range Test for GLU NOTE: This test controls the Type I comparisonwise error rate, not the experimentwise error rate Alpha 0.05 Error Degrees of Freedom Error Mean Square 0.000047 Number of Means Critical Range 004566 004732 Means with the same letter are not significantly different Duncan Grouping Mean N DC A 0.553333 3 B 0.394667 C 0.334000 - Thay doi hoat enzyme glucanase giua cac cong thuc khac sau The ANOVA Procedure XXXII Duncan's Multiple Range Test for GLU NOTE: This test controls the Type I comparisonwise error rate, not the experimentwise error rate Alpha 0.05 Error Degrees of Freedom Error Mean Square 0.000343 Number of Means Critical Range 004939 005119 Means with the same letter are not significantly different Duncan Grouping Mean N DC A 0.543000 3 B 0.322667 C 0.313000 - Thay doi hoat enzyme chitinase cung cong thuc E I The ANOVA Procedure Duncan's Multiple Range Test for CHI NOTE: This test controls the Type I comparisonwise error rate, not the experimentwise error rate Alpha 0.05 Error Degrees of Freedom Error Mean Square 0.000124 Number of Means Critical Range 004922 005129 005245 Means with the same letter are not significantly different Duncan Grouping Mean N DC A 0.253667 3 B 0.234667 C 0.213333 D 0.193667 - Thay doi hoat enzyme chitinase cung cong thuc E II The ANOVA Procedure Duncan's Multiple Range Test for CHI NOTE: This test controls the Type I comparisonwise error rate, not the experimentwise error rate Alpha 0.05 Error Degrees of Freedom Error Mean Square 0.00004 Number of Means Critical Range 01193 01244 01272 Means with the same letter are not significantly different Duncan Grouping Mean N DC A 0.403333 3 B 0.352000 C 0.310000 D 0.223667 - Thay doi hoat enzyme chitinase cung cong thuc E III The ANOVA Procedure Duncan's Multiple Range Test for CHI NOTE: This test controls the Type I comparisonwise error rate, not the experimentwise error rate Alpha 0.05 XXXIII Error Degrees of Freedom Error Mean Square 0.00052 Number of Means Critical Range 003394 003537 003617 Means with the same letter are not significantly different Duncan Grouping Mean N DC A 0.513000 3 B 0.492667 C 0.423333 D 0.252667 - Thay doi hoat enzyme chitinase giua cac cong thuc khac sau The ANOVA Procedure Duncan's Multiple Range Test for CHI NOTE: This test controls the Type I comparisonwise error rate, not the experimentwise error rate Alpha 0.05 Error Degrees of Freedom Error Mean Square 0.00034 Number of Means Critical Range 004212 004365 Means with the same letter are not significantly different Duncan Grouping Mean N DC A 0.253000 3 B 0.224000 C 0.193667 - Thay doi hoat enzyme chitinase giua cac cong thuc khac sau The ANOVA Procedure Duncan's Multiple Range Test for CHI NOTE: This test controls the Type I comparisonwise error rate, not the experimentwise error rate Alpha 0.05 Error Degrees of Freedom Error Mean Square 0.023123 Number of Means Critical Range 004264 004420 Means with the same letter are not significantly different Duncan Grouping Mean N DC A 0.423000 3 B 0.303333 C 0.212333 - Thay doi hoat enzyme chitinase giua cac cong thuc khac sau The ANOVA Procedure Duncan's Multiple Range Test for CHI NOTE: This test controls the Type I comparisonwise error rate, not the experimentwise error rate Alpha 0.05 XXXIV Error Degrees of Freedom Error Mean Square 0.00015 Number of Means Critical Range 004566 004732 Means with the same letter are not significantly different Duncan Grouping Mean N DC A 0.513667 3 B 0.404333 C 0.253000 - Thay doi hoat enzyme chitinase giua cac cong thuc khac sau The ANOVA Procedure Duncan's Multiple Range Test for CHI NOTE: This test controls the Type I comparisonwise error rate, not the experimentwise error rate Alpha 0.05 Error Degrees of Freedom Error Mean Square 0.00024 Number of Means Critical Range 003460 003586 Means with the same letter are not significantly different Duncan Grouping Mean N DC A 0.492667 3 B 0.352667 C 0.234333 XXXV H v Nguy ng 23/02/1985 a ch li Ngh An c: i h c Hu Hu , Th NH O - 2004-2008: H qu n ch bi ih ct o n th c ph m - 2012-2014: H c cao h c t th c ph - n nay: Gi u ng ... gloeosporioides t thu ho ch n ng n nh t h i l n v kinh t [62] h n ch b nh i thu c s d ng [10], [45] th n s c kh t ch ph u qu i rau qu sau thu ho c h n ch t n e (TBZ) cs d i thu nv ng, ng ct t sau thu ho... i mong mu n m b nh h i t sau thu ho u ng d u so v i xu t m t gi c hi b i t sau i t sau thu ho ch c a thu ho M u - nh kh nanochitosan - ng d b N i t u - T o ch ph m nanochitosan b Sodium tripolyphosphat... p, h t nanochitosan tn n, c h t nh n th h c l a ch n ph bi u ng d ng nanochitosan [23] Khu y t cao S H t nanochitosan t o h t nanochitosan b o gel ionic 25 Vi c s d ph m sinh h ki nh sau thu ho