Cloning in Saccharomyces cerevisiae

... moleculeprotein interaction, resulting in activation of the downstream reporter gene Screening for compounds that inhibit a protein-protein interaction URA3 +5-FOA URA3 + 5-FOA (A) An interacting protein ... same PREY against two different BAITS Small molecule yeast two-hybrid screening (A) A scaffold bait is constructed by fusing a DNA binding domain (DBD) to a small molecule binding prot...

Ngày tải lên: 15/06/2017, 19:56

... Bhagwat SV, Biswas G, Anandatheerthavarada HK, Addya S, Pandak W & Avadhani NG (199 9) Dual targeting property of the N-terminal signal sequence of P450 1A1 Targeting of heterologous proteins to ... open N-terminal signal domain is critical for protein targeting to the ER (Fig 2D) In contrast to the targeting of CYP 1A1 requiring N-terminal truncation, w...

Ngày tải lên: 18/02/2014, 16:20

... to the 9th cycle from the N-terminus including the initiating Met: MHKTHSTMS for Sno1p and MTGEDFKIKS for Snz1p Properties of the complex of Sno1p and Snz1p When Sno1p with a His-tag and Snz1p ... SNO3, SNZ2 and SNZ3 complement the defect The relationship of all of these genes remains to be clarified Expression and purification of Sno1p and Snz1p In light...

Ngày tải lên: 19/02/2014, 12:20

... strain was grown in media lacking leucine The other strains were grown in media containing leucine Complementing abilities of LEU2, UTR1 and YEF1 for the growth defects of the pos5 mutant To confirm ... using recombinant protein expressed in E coli We also confirmed that Utr1p, initially identified as an ATP-NAD kinase [1], was in fact an ATP-NADH kinase Thus, the three is...

Ngày tải lên: 20/02/2014, 01:20

... [6,7] In this study, Nop53p was isolated in a two-hybrid screen as a protein interacting with Nop17p, which is involved in the early steps of prerRNA processing [28] Nop17p and Nop53p interacted in ... established a functional link between several proteins involved in prerRNA processing The exosome subunit Rrp43p interacts with Rrp46p, Nip7p and Nop17p [10,13,...

Ngày tải lên: 20/02/2014, 01:20

... for binding of wild-type Ste12 to a PRE consensus (ATGAAACA) in vitro FUS1 PREa Sequence RCSb II IV ATGAAACA tTGAAACA AaGAAACA ATaAAACA ATcAAACA ATtAAACA ATGcAACA ATGgAACA ATGAgACA ATGAAgCA ATGAAAgA ... This indicates that there are severe organizational constraints for closely-positioned PREs that must limit A B Fig Organizational constraints on closely-spaced PREs for...

Ngày tải lên: 06/03/2014, 22:21

... because the side chain of Val216 interfere with binding of a 4-linked glucose The CG1 of Val216 is too close to the O3¢ of maltose On the other hand, both isomaltose and maltose can bind to the V216T ... fragment of pYIM in pYMA were the same as those of Mun/Bst Therefore, these results indicate that the fragment including consensus region II contributes to th...

Ngày tải lên: 07/03/2014, 16:20

... to validate the mechanism of induction of GAL genes by galactose In each of the models, cytoplasmic Gal3 p is activated by galactose Further, Gal4 p dimerizes and interacts with the DNA to form the ... expression of GAL genes in response to galactose The GAL genetic switch The GAL regulatory network is composed of three regulatory proteins:...

Ngày tải lên: 07/03/2014, 16:20

... LJY430 LJY431 LJY432 MATa MATa MATa MATa MATa MATa MATa MATa MATa MATa MATa MATa MATa MATa MATa MATa MATa MATa MATa MATa MATa MATa MATa MATa MATa MATa MATa MATa MATa Euroscarf [16] ATCC [15] This study ... the yeast deubiquitinating enzyme Ubp16, a metalloprotease belonging to the large family of deubiquitinating enzymes, mainly consisting of cysteine proteases [43] Another class of p...

Ngày tải lên: 07/03/2014, 16:20

... presence of < /b> stigmatellin, which binds in < /b> the < /b> distal lobe of < /b> the < /b> Qo < /b> site,< /b> and myxothiazol, azoxystrobin and pyraclostrobin, which bind at the < /b> proximal lobe of < /b> the < /b> Qo < /b> site < /b> (Fig and Table 1) The < /b> control ... no growth Growth on Strains AB4 AB13 AB5 AB18 AB7 AB8 AB9 AB10 AB16 AB11 AB12 Myxothiazol 10 Stigmate...

Ngày tải lên: 16/03/2014, 16:20

... display a wide spectrum of glucose uptake rates These strains are therefore useful for investigating the effects of different glycolytic rates on glucose- induced signalling pathways For this study ... 5A), indicating that the protein was phosphorylated to a different extent and was partially inactive as a repressor Interestingly, Mig1 from cells growing in the presence of 0....

Ngày tải lên: 16/03/2014, 18:20

... described in Materials and methods H2O2 evokes a decrease and an increase in the intracellular concentration of ATP and inosine, respectively Searching for the rationale for these phenomena, possible ... degradation and the appearance of intermediate products were essentially the same in both cases and, above all, no appreciable differences in the rates o...

Ngày tải lên: 17/03/2014, 10:20

... SIN3 leads to an advanced induction of Start- specific transcription in G1 daughter cells and that budding is initiated at a smaller cell size Consistent with a direct role for Sin3 in repressing ... to approximately the size of sin3D in the double mutant, suggesting that sin3 is partly epistatic to cln3 (Fig 3A–C) The critical cell size for the initiation of bud...

Ngày tải lên: 23/03/2014, 05:22

... Temperature-dependent ricin A chain mutants Fig Stability of mutant ricin A chains The kinetics of protein degradation of (A) Kar2SP-RTAE177D and (B) Kar2SP-RTAF108L ⁄ L151P at all temperatures, or a cytosolic ... 5¢-ATATTCCCCAAACAATACCC-3¢ and the antisense primer CP133 5¢-TTAAAACTGTGACGATGGT GGA-3¢ with the TAA termination anticodon shown in bold Amplification react...

Ngày tải lên: 23/03/2014, 07:20

... and the function of mitochondrial FAD- binding enzymes Correlation of these has been demonstrated in human pathologies, including deficiencies of the flavoprotein subunit of respiratory chain complex ... be flavinylated either in the WTHA strain or in the flx1D-HA strain These experiments also showed that the availability and attachment of flavin cofactors are no...

Ngày tải lên: 23/03/2014, 07:20