expression of vhhs in saccharomyces cerevisiae
Aerobic Uptake of Cholesterol by Ergosterol Auxotrophic Strains in Candidaglabrata & Random and Site-Directed Mutagenesis of ERG25 in Saccharomyces cerevisiae
... starting temperature of 195˚C for one minute increasing to 240˚C in 20˚C/min increments and then 2˚C/min increments to a final temperature of 280˚C where the temperature held for five minutes ... 3.3 GC profile of ERG7 in Candida glabrata 41 3.4 GC profile of ERG11 in Candida glabrata 42 3.5 GC profile of ERG25 in Candida glabrata 43 3.6 GC profile of ERG27 in Candida ... was used with a starting temperature of 100˚C for one minute increasing to 300˚C in 7˚C/min increments and then held for 15 minutes at the final temperature of 300˚C The injection volume per...
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The investigation of transcription related protein protein interactions of histones in saccharomyces cerevisiae
... THE INVESTIGATION OF TRANSCRIPTION-RELATED PROTEIN-PROTEIN INTERACTIONS OF HISTONES IN SACCHAROMYCES CEREVISIAE ZHAO JIN (M Sc (Molecular Engineering of Biological and Chemical ... genetic in vivo approach to detect interacting proteins in living yeast cells It is based on expressing one protein as a fusion to a DNA-binding domain of a transcriptional activator and expressing ... H4 alanine mutant proteins in place of wild-type H4 53 Figure 3.6 Antimycin A sensitivity of histone H4 alanine point mutant strains 54 Figure 3.7 3-Aminotriazole sensitivity of histone...
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Báo cáo khoa học: Succinate dehydrogenase flavoprotein subunit expression in Saccharomyces cerevisiae – involvement of the mitochondrial FAD transporter, Flx1p ppt
... quantitation of microgram quantities of protein utilizing the principle of protein-dye binding Anal Biochem 72, 248–254 55 Laemmli UK (1970) Cleavage of structural proteins during the assembly of the ... be flavinylated either in the WTHA strain or in the flx1D-HA strain These experiments also showed that the availability and attachment of flavin cofactors are not involved in the regulation of Sdh1p ... carrier protein involved in maintaining a proper balance of flavin nucleotides in yeast mitochondria J Biol Chem 271, 7392–7397 16 Dibrov E, Fu S & Lemire BD (1998) The Saccharomyces cerevisiae...
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Báo cáo khoa học: Dynamin-like protein-dependent formation of Woronin bodies in Saccharomyces cerevisiae upon heterologous expression of a single protein pdf
... formed in yeast which is likely to contain only minor inclusions of peroxisomal matrix proteins A C Vps1p and Dnm1p: two dynamin-like proteins involved in fission of peroxisomes and Woronin bodies ... was conducted, in the presence or absence of detergent Examination of a wild-type PNS revealed increasing amounts of peroxisomal marker proteins in the pellet fractions upon increasing centrifugation ... numbers of Woronin bodies were reduced [21] It will be interesting to see whether the absence of both dynamin-like proteins prevents the fission of Woronin bodies in filamentous fungi It is worth noting...
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Báo cáo khoa học: "Expression of apxIA of Actinobacillus pleuropneumoniae in Saccharomyces cerevisiae" docx
... Haesebrouck, F Effect of endobronchial challenge with Actinobacillus pleuropneumoniae serotype of pigs vaccinated with a vaccine containing Apx toxins and transferrin-binding proteins J Vet Med B 2001, ... membranes were incubated in 5% skim milk (Sigma Co.) in Tris buffered saline (TBS, pH 7.5) for hrs at 37oC Expression of apxIA of Actinobacillus pleuropneumoniae in Saccharomyces cerevisiae 227 ... immunity in mice Infect Immun 2002, 70, 6464-6467 21 Shin, S J., Cho, Y W and Yoo, H S Cloning, sequencing and expression of apxIA, IIA, IIIA of Actinobacillus pleuropneumoniae isolated in Korea...
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Tài liệu Báo cáo khoa học: Mitochondrial targeting of intact CYP2B1 and CYP2E1 and N-terminal truncated CYP1A1 proteins in Saccharomyces cerevisiae ) role of protein kinase A in the mitochondrial targeting of CYP2E1 pdf
... Role of PKA in the mitochondrial targeting of CYP2E1 The role of PKA in mitochondrial targeting of CYP2E1 was investigated using two approaches The first approach involved measuring the level of ... ferredoxin (FDX) + ferredoxin reductase (FDXR), homologs of mammalian ferredoxin and ferrodoxin reductase [27,28] Results Expression of intact and truncated rat CYPs in Saccharomyces cerevisiae ... targeting of CYP2E1 protein Mitochondrial targeting of CYP2B1 in yeast cells We used the protease-deficient strain pep4D for testing the mitochondrial targeting of rat CYP2B1 The western blot in...
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Tài liệu Báo cáo khóa học: Characterization of the products of the genes SNO1 and SNZ1 involved in pyridoxine synthesis in Saccharomyces cerevisiae pptx
... relationship of all of these genes remains to be clarified Expression and purification of Sno1p and Snz1p In light of the similarity in the amino acid sequence of Sno1p to that of the glutaminase subunit of ... consistently pointed to the formation of glutamate, proving unambiguously that the complex did indeed hydrolyze glutamine efficiently Kinetics of the glutaminase reaction The kinetics of glutamine hydrolysis ... Srinivasan, M., Pochart, P et al (2000) A comprehensive analysis of protein– protein interactions in Saccharomyces cerevisiae Nature 403, 623–627 24 Mei, B & Zalkin, H (1989) A cysteine-histidine-aspartate...
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Tài liệu Báo cáo khoa học: Identification of ATP-NADH kinase isozymes and their contribution to supply of NADP(H) in Saccharomyces cerevisiae docx
... 4.9 a This strain was grown in media lacking leucine The other strains were grown in media containing leucine Complementing abilities of LEU2, UTR1 and YEF1 for the growth defects of the pos5 mutant ... slightly inhibited Yef1p; however, NADP and intermediates involved in NAD biosynthesis (nicotinic acid mononucleotide, nicotinic acid adenine dinucleotide, nicotinic acid and quinolinic acid) ... complementing ability of LEU2 for the growth defects of the pos5 mutant, we examined the growth of pos5 cells containing CgLEU2 instead of POS5 on the chromosome, ScLEU2 (LEU2 of S cerevisiae) ...
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Báo cáo khoa học: Val216 decides the substrate specificity of a-glucosidase in Saccharomyces cerevisiae doc
... because the side chain of Val216 interfere with binding of a 4-linked glucose The CG1 of Val216 is too close to the O3¢ of maltose On the other hand, both isomaltose and maltose can bind to the V216T ... nucleophile in consensus region II must be involved in the recognition of a-glucosidic linkages In conclusion, this work was successful in identifying the region and residue important in the determination ... clones expressing isomaltase was selected The plasmid containing the isomaltose gene was designated pYIM Cloning of the maltase gene from S cerevisiae For cloning of the maltase gene, S cerevisiae...
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Báo cáo khoa học: A steady-state modeling approach to validate an in vivo mechanism of the GAL regulatory network in Saccharomyces cerevisiae ppt
... sequences of the GAL genes through its N-terminal DNA binding site Gal80p inhibits the transcriptional activity of Gal4p by binding to its 28 amino acid region at the carboxyl terminal [25–27] In vitro ... reflected by the sequence of interactions; (c) parameters quantifying strength of interactions; and (d) spatial localization of protein in a compartment or shuttling of proteins between compartments ... concentration of Gal3p in the wild-type thus reducing the protein expression as in Model III This confirms the evidence obtained through gel filtering and cross-linking that Gal3p is monomeric in solution...
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Báo cáo khoa học: Enhanced peptide secretion by gene disruption of CYM1, a novel protease in Saccharomyces cerevisiae doc
... processing sites for Cym1p Expression of these proteins as well as proCCK in the yps1 cym1 double mutant showed an increase in the amount of secreted protein, indicating that Cym1p processes all of ... large family of deubiquitinating enzymes, mainly consisting of cysteine proteases [43] Another class of proteins are the tail-anchored proteins, which include the translocases of the mitochondrial ... cleavage in CCK-22 maturation in vitro is dependent on the amount of Cym1p Enhanced CCK secretion in a cym1D0 strain To elucidate the role of Cym1p in the biosynthesis of CCK22 in vivo, both the intracellular...
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Báo cáo khoa học: Modeling the Qo site of crop pathogens in Saccharomyces cerevisiae cytochrome b ppt
... structure of the Qo site, which can now accommodate azoxystrobin but not pyraclostrobin The sidechain of F275 in chicken bc1 complex is involved in a stabilizing ring–stacking hydrophobic interaction ... cytochrome b and is involved in interactions with the peripheral domain of the iron–sulphur protein The stigmatellin head-group binds in this distal lobe of the Qo site and is positioned in a pocket ... azoxystrobin and pyraclostrobin: strains AB2, AB13, AB8 and AB10 grew on nonfermentable medium in presence of 10 lM of each of these compounds but were still sensitive to stigmatellin Interestingly...
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Báo cáo khoa học:The isolation and characterization of temperature-dependent ricin A chain molecules in Saccharomyces cerevisiae docx
... [7] To gain a more complete inventory of factors required for the entry of ricin A chain to the cytosol it will be useful to express inducible toxin in the ER of mutant strains of yeast, in a manner ... yeast, in a manner akin to its expression in plant cells [18] Survivors of toxin expression may contain defects in genes normally essential for toxin retro-translocation, refolding, degradation or ... they had been depurinated by active toxin in vivo determined (this is designated as time in Fig 4) The remainder of each culture was divided into two, with one half being incubated at the permissive...
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Báo cáo sinh học: "Comprehensive curation and analysis of global interaction networks in Saccharomyces cerevisiae" doc
... potential bias in the extent of investigation of any given node in the LC datasets, we determined the number of total interactions (that is, including redundant interactions) in excess of connectivity ... over 100 random trials, in which the interactions of both networks are reassigned while maintaining the same number of interactions per protein, resulting in a distribution of random subnetwork ... Genetic interactions with RNA-encoding ORFs were not scored separately from protein-coding genes In rare instances in which an interaction could not be readily assigned a protein or genetic interaction...
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Population genetic variation in gene expression is associated with phenotypic variation in Saccharomyces cerevisiae pdf
... Identification of gene expression differences in the presence and absence of copper sulfate Expression levels were measured using DNA microarrays in the nine strains during exponential growth in rich ... different levels in the presence of copper sulfate, 20 of which showed a twofold or greater level of expression in the presence of copper sulfate and one, ZRT1, encoding a high-affinity zinc transporter, ... O'Donovan MC: Cis-acting variation in the expression of a high proportion of genes in human brain Hum Genet 2003, 113:149-153 Rifkin SA, Kim J, White KP: Evolution of gene expression in the Drosophila...
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Báo cáo y học: " Replication and transcription of human papillomavirus type 58 genome in Saccharomyces cerevisiae" pdf
... Therefore, E2 protein could facilitate viral genome replication and maintenance Role of E2 protein in maintaining HPV58 genome in yeast Results Episomal replication of HPV58 in yeast Viral DNA ... maintain the mitotic stability of its genome in an E2-independent manner in yeast and mammalian cells [7,20,21] In contrast to HPV16 genome, the maintenance of HPV58 genome depends on E2 protein ... specific maintenance pattern of the HPV58 genome may provide a possible way to interfere with the early stage of HPV58 infection by blocking the expression of E2 protein Previous reports of the HPV/yeast...
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Báo cáo y học: " Analysis of HIV-1 Vpr determinants responsible for cell growth arrest in Saccharomyces cerevisiae" ppt
... is indicated on the right (+) indicates efficient binding while (-) indicates absence of binding inducing growth arrest in budding yeast and strongly suggest that the mechanism(s) underlying ... domain encompassing Vpr amino acids 15–27 and 63–77 was also shown to be involved in UDG binding [50] Based on these observations, it appears that similar regions of Vpr are involved in binding ... activity in budding yeast, indeed suggesting that Vpr carries out distinct functions in S cerevisiae Results Analysis of Vpr sequence and/or structural determinants implicated in the interaction...
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Báo cáo y học: "Spore germination in Saccharomyces cerevisiae: global gene expression patterns and cell cycle landmarks" potx
... from those in resting spores, in the first phase of incubation in glucose or in the process of normal spore germination in YPD medium To further characterize the role of glucose in inducing the ... germination in rich medium (YPD) Germination of spores preincubated in glucose Germination of spores preincubated in ‘nitrogen’ Germination transcription program in spores pre-incubated in glucose ... Protein of unknown function CSI2 Protein involved in chitin synthesis MSH2 Component with Msh3p and Msh6p of DNA mismatch binding factor, involved in repair of single base mismatches and short insertions/deletions...
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Báo cáo y học: "High-resolution analysis of condition-specific regulatory modules in Saccharomyces cerevisiae" pptx
... Msn2, are known to have a role in influencing the accessibility of promoters [50,51] Interestingly, our results indicated distinct and interesting hypotheses regarding the condition-specific regulatory ... Effectors of a developmental mitogen-activated protein kinase cascade revealed by expression signatures of signaling mutants Proceedings of the National Academy of Sciences of the United States of America ... rewiring of the transcriptional regulatory network In addition, many of the functional gene groups that exhibited distinct expression profiles in other conditions were coexpressed in a certain...
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Báo cáo y học: " Characterization of the yeast ionome: a genome-wide analysis of nutrient mineral and trace element homeostasis in Saccharomyces cerevisiae" ppsx
... above Strains carrying mutations in 21 of the 27 genes identified in our study that are involved in vacuolar biogenesis and function showed marked increases in sulfur levels The underlying molecular ... Biogenesis of the vacuole in Saccharomyces cerevisiae Int Rev Cytol 1992, 139:59-120 Ooi CE, Rabinovich E, Dancis A, Bonifacino JS, Klausner RD: Copper-dependent degradation of the Saccharomyces cerevisiae ... genes identified by ionome profiling of their Functional classes of genes identified by ionome profiling of their corresponding mutants The number of genes identified in each functional class is represented...
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