transcription of rdna in the yeast saccharomyces cerevisiae

... acyltransferase was determined in an are1 deletion background The enzyme activity in the ste20D strain and the cla4D strain was indistinguishable from that in the wild-type strain (Fig 7A) Therefore, it ... elicit a mating response in cells of the opposite mating type Cdc42 is involved in pheromone signalling that eventually results in G1 arrest and the formation of a mating projection [6] Furthermore, ... to the PAK Released RUra3, a modified version of the enzyme Ura3 with an arginine at the extreme N-terminus, is targeted for degradation by the enzymes of the N-end rule A protein–protein interaction...

Ngày tải lên: 18/02/2014, 13:20

... functions of the yeast oxysterol-binding protein homologs Genetics 157, 1117–1140 Beh CT & Rine J (2004) A role for yeast oxysterol-binding protein homologs in endocytosis and in the maintenance of intracellular ... provided invaluable insights into understanding the function of this family of proteins in yeast and offered guidance to future research On the other hand, although the entire Osh protein family shares ... Osh6p may bind phospholipids through a domain other than PH In support of this, the short Kes1p containing no PH domain was also shown to bind PtdIns (4,5)P [15] Although the role of the conserved...

Ngày tải lên: 07/03/2014, 21:20

... of Ark1/Prk1 kinase domain structures In all cases, the kinase domain is near the amino terminus (red lozenge) The length of the region downstream of the kinase domain is extremely variable In ... endocytosis in the yeast Saccharomyces cerevisiae 1.4.1 Actin structures in yeast 1.4.2 Endocytosis in yeast 10 1.4.3 Actin involvement in yeast endocytosis 13 1.4.4 A dynamic picture of yeast actin and ... by the actin binding proteins The biochemical features of actin, together with the actin binding proteins, allow actin filament to work as a physical force when the polymer grows beneath the...

Ngày tải lên: 12/09/2015, 08:19

... in coat disassembly Inhibiting the kinases Ark1p and Prk1p leads to the accumulation of clusters of actin filamentcovered endocytic vesicles, highlighting the necessity of uncoupling the actin ... phosphoregulation of actin-driven endocytosis [Chapter 5] This finding completes the other half of the cycle of phosphoregulation of endocytosis and, therefore, is an important advancement in the field of endocytosis ... functions of different actin-cytoskeleton proteins during endocytic internalization in S cerevisiae Las17p, the yeast homology of Wiskott–Aldrich syndrome protein [WASP] together with the myosins Myo3p...

Ngày tải lên: 14/09/2015, 09:44

... 2D), suggesting that, at least in part, the Ca2+ flux comes from outside the cells Following the addition of BAPTA, the pattern of the luminescence spectrum recorded for the WT strain changed from ... responsible for release of Ca2+ from the vacuole to the cytosol The YVC1 gene deletion drastically reduced the amplitude of the Ca2+ burst, indicating that most of the Ca2+ released into the cytosol after ... by the addition of BAPTA (Fig 2H), suggesting that the small Ca2+ burst seen in yvc1D cells was the result of Ca2+ in ux from outside the cell As BAPTA addition removes  50% of the signal in the...

Ngày tải lên: 15/03/2014, 23:20

... indicating that the current transitions are substates of a single channel rather than amplitudes of several independent channels inserted in the membrane The flickering of the channel resembles the behaviour ... function of the yeast peroxisomal membrane predicts that it contains poreforming proteins that allow the free diffusion of small solutes The channel-forming activity in yeast peroxisomes To detect the ... detected in the mitochondrial fraction (compare the upper and lower panels in Fig 2C), indicating that the pore-forming activity is determined by peroxisomal proteins but not by contaminating proteins...

Ngày tải lên: 30/03/2014, 02:20

... Ampr) carrying GTS1 in- frame downstream of the DNA-binding domain of GAL4 To determine the binding site of Gts1p and GAPDH, the wild-type and mutant GTS1 genes were inserted into pGBT9, and the cloned ... Determination of the binding site of Gts1p in the 97 C-terminal residues of GAPDH3 (GAPDH3-C97) using N-terminal and C-terminal deletion mutants (A), and mutants with modifications in the zinc finger ... coupled to the metabolic oscillation Deletion of GTS1 resulted in the loss of the fluctuations in the synthesis of trehalose and cAMP [26], leading to the disappearance of the oscillations These results...

Ngày tải lên: 31/03/2014, 21:21

... the kinase activity of the cyclin dependent kinase, Cdc28, by altering its association with any of the nine cyclins in Saccharomyces At the beginning of G1, Cdc28 associates with the G1 cyclin, ... attachment of the C-terminal glycine of a free ubiquitin to an acceptor lysine on the substrate associated ubiquitin 35 Initial investigations primarily focused on the mechanism of lysine 48 linkage ... categories based on the presence of a RING (really interesting new gene) finger domain or a HECT (homologous to E6-AP carboxyl terminus) domain containing protein in the complex 37 Perhaps the best characterized...

Ngày tải lên: 24/08/2014, 13:29

Ngày tải lên: 14/11/2014, 06:20

... limiting membrane of the endosome is sorted into these invaginating vesicles Subsequent fusion of the mature MVB with the vacuole results in the delivery of the internal vesicles, along with their ... structure of Osh7p The black box indicates the oxysterol-binding domain while the gray box indicates the coiled-coil domain N and C denote the N- and C- terminals of the protein 40 1.7 OBJECTIVES OF ... 2001) The homology of all the seven proteins was the highest in a small domain of 150 to 200 amino acids This small domain is known as the OSBP domain Beside the OSBP domain, these proteins also...

Ngày tải lên: 03/10/2015, 20:57

... Oxidation of thymine in thymidine and its nucleotides The kinetics of oxidation of thymine by KMnO4 [34] in thymidine, TMP, TTP and TpT was studied as a function of [MgCl2] in the range 0100 mm in the ... observation, conrming that the mechanism underlying the Mg2+ effect on the kinetics of nucleotide oxidation does not affect the intrinsic rate of the reaction and is due solely to the screening of the negative ... band doubling for T)11 and T+2 Therefore, the measured contribution of these forms to the integrated intensity of these two bases was used in the calculation of the contribution of individual...

Ngày tải lên: 19/02/2014, 18:20

... is implicated in the constitutive expression of fgl2 to the induced transcription of fgl2 in response to MHV-3 We examined whether a mutation of the PRD would abolish the induction of fgl2 by MHV-3 ... protein, possibly necessary for development of a normal fetus and in regulating immune responses within the intestine Induction of fgl2 in macrophages in response to viruses results in the production ... Fig Arrows indicate the bands that are interacting with these probes All the lanes have labelled probes The adding of extracts and cold probe are indicated in the top of the panel Supershift,...

Ngày tải lên: 20/02/2014, 11:20

... shown in light green, the compensatory mutations are shown in pink, and the hinge region of the ISP is shown in white the integration or stability of the ISP within the bc1 complex In order to investigate ... enzyme with their ISP in different conformations The mutation G167E could distort the hinge region of the ISP and hinder the movement of the headgroup Mutations in the hinge region of the ISP, which ... amplitude, 1.6 mT ISP–cytochrome b interaction at the hinge region Movement of the extrinsic domain of the ISP is facilitated by the unwinding of a single turn of the 310 helix formed from ˚ residues...

Ngày tải lên: 07/03/2014, 15:20

... by comparing the results of the SDS ⁄ PAGE that was done after the zymogram staining with those of the SDS ⁄ PAGE of the active fractions after the DEAE column and correlating them with the enzyme ... vector in the same yeast strain When testing the two modified proteins in the crude extract of S cerevisiae, we observed that the N-terminally tagged enzyme did not exhibit any activity The C-terminally ... histidine-tagged enzymes were generated in a similar way The coding sequences for six histidines were introduced by PCR either at the N-terminus or at the C-terminus To introduce the histidine-tag...

Ngày tải lên: 30/03/2014, 04:20

... including NTG1 [23], DNA2 [24], DIN7 [25], were also in this group Group (474 transcripts) increased by the first time point and began to decrease within two to To determine the rate of the initial ... and used to print the slides During printing, the relative humidity was maintained between 50% and 52% and the ambient temperature was maintained between 21°C and 23°C After printing, slides were ... activation of transcription and translation Interestingly, there is a 40% overlap between transcripts that increase by minute in this study and transcripts that increase by the first time point (10 minutes)...

Ngày tải lên: 14/08/2014, 16:21

Ngày tải lên: 14/08/2014, 17:22

... [19,20] Of the latter, only the role of the DRE in the recruitment of the polymerase has been unraveled [44] We evaluated the occurrence of these eight motifs and their most frequently occurring ... correspond to the strength of location biases of the promoter elements that define them With the exception of the INR, there were fewer occurrences of the location-specific canonical elements in peaked ... upstream of TSSs in the EPD and our sets, highlighting the bias in the usage of the most 5' evidence as TSSs, rather than the most highly utilized locations Looking at the presence of sequence...

Ngày tải lên: 14/08/2014, 21:20

... regulator of cytokinesis Cells overexpressing Nuc2p phenocopied sin mutants in that the actomyosin rings were not maintained upon completion of mitosis Examination of SIN proteins in the temperature-sensitive ... is the primary motor protein responsible for cytokinesis The contractile rings of animal cells are composed of several actin binding proteins such as alpha-actinin, tropomyosin, anillin, filamin, ... cytokinesis in fission yeast, in addition to its role in ensuring kinetochore-spindle attachment Inactivation of the SAC in cells defective in chromosome segregation leads to cytokinesis in the...

Ngày tải lên: 11/09/2015, 09:06

... disassembly of the spot prevent actomyosin ring assembly, implying that the spots may be the precursor of the astomyosin ring (Wong et al 2002) Prior to the formation of the actomyosin ring many of the ... ring producing two daughter cells of equal size The actomyosin ring consists of about 50 different proteins including actin, actin regulatory proteins, type II myosin heavy and light chains The ... microinjection of oil in dividing sea urchin eggs (Hiramoto 1965) The droplet was constricted by the ingressing furrow, indicating that a force larger than the surface tension of the oil droplet in...

Ngày tải lên: 14/09/2015, 14:18

... identification of lipid species 32 Lipid profiling of single deletion mutant strains 33 3.3.1 Lipid profiling of strains in a random manner 33 3.3.2 Lipid profiling of strains in a domain based manner ... component Involved in lipid metabolism Sphingoid long chain base Diacyl glycerol kinase kinase Sphingosine Kinase Minor Sphingoid long chain Diacyl glycerol kinase kinase Sphingosine Kinase NADPH-dependent ... axis of one of the profiles using the other profile as the reference One of the replicate spectra from the control condition is chosen as the reference to which the other spectra are warped The...

Ngày tải lên: 04/10/2015, 16:03