Role of neutrophil NADPH oxidase derived reactive oxygen species (ROS) in innate immune responses

... looked for an involvement of NADPH oxidase derived ROS in the recruitment of neutrophils to sites of inflammation in vivo Since the gene coding for the gp91 subunit of the NADPH oxidase is on ... INNATE IMMUNE SURVEILLANCE 1.1 Sentinel systems of innate immunity 1.2 Innate system effector cells PRODUCTION OF ROS OF CELLS OF THE INNATE IMMUNE SYSTEM ....

Ngày tải lên: 21/05/2016, 22:38

... esophageal cells resulting in esophageal adenocarcinoma (EA) Acid reflux damage is a major factor contributing to the transformation of EA NOX5-S was found to be the major NOX5 isoform present in ... is able to activate/ modulate signal transduction pathways such as the MAP kinase signaling pathways, JAK/STAT signaling pathways, PI3K/Akt pathway and cAMP/cGMP signaling p...

Ngày tải lên: 26/09/2015, 10:44

... system [13], either by measurement of the rate of production of O2– or the rate of O2 uptake INT diaphorase activity was assayed by the rate of reduction of INT into formazan in the presence of ... The quantity of neutrophil membranes used in the oxygraphic assays was 10 · that used in the photometric assay For measurement of the Km of activated oxid...

Ngày tải lên: 31/03/2014, 21:21

... down-regulated both in vitro and in vivo Moreover, Hp induces genomic instability in nuclear CA repeats in mice and in mtDNA of AGS cells and chronic gastritis tissue, and this effect in mtDNA is associated ... Hp infection resulted in increased mutations in the non-coding D-loop as well as the coding genes ND1 and COI of mtDNA of gastric cells [12] The increase...

Ngày tải lên: 25/10/2012, 11:18

... Structure, regulation and evolution of Nox H Sumimoto Introduction Reactive oxygen species (ROS) are conventionally regarded as inevitable deleterious byproducts of aerobic metabolism ... enzymes, and not EF-hand-containing oxidases such as Nox5 and Duox, although these two families are found in a variety of species of protostomes and deuterostomes (Fig 3) Thus...

Ngày tải lên: 16/03/2014, 06:20

... preparation, statistical analyses and drafted the manuscript A- KL participated in the design of the study and the statistical analyses HK and LA performed the logistic regression analysis LP and LK were ... 71% and 72%, respectively, are female; the mean age was 51 ± 13 years in cases and 54 ± 12 in controls Information about RF and anti-CCP antibody status was availab...

Ngày tải lên: 09/08/2014, 10:21

... SCHOOL Research Integrity and Copyright Disclaimer Title of Thesis/Dissertation: Bone Metabolism: The Role of STAT3 and Reactive Oxygen Species For the degree of Master of Science Choose your degree ... http://www.purdue.edu/policies/pages/teach_res_outreach/c_22.html BONE METABOLISM: THE ROLE OF STAT3 AND REACTIVE OXYGEN SPECIES A Thesis Subm...

Ngày tải lên: 24/08/2014, 12:59

... investigate the physiological relevance of Noxo1c expression in the testis, we examined expression of Nox1 and Noxa1 by PCR analysis and found a small but significant amount of the Nox1 and Noxa1 mRNAs ... al Expression and function of Noxo1c A B C Fig Alternative splice forms of human Noxo1 (A) The genomic organization of human NOXO1 gene Translated seque...

Ngày tải lên: 19/02/2014, 06:20

... (Figs 3–5) Antioxidant defences of q0 cell lines GSH-GSSG and GR We measured GSH and GSSG in exponentially growing cells, as GSH content changes in the growth and lag phases [51] In all q0 cells studied, ... cells, and that there are cell line-to -cell line variations in intracellular antioxidant defences and ROS homeostasis In fact among the studied cel...

Ngày tải lên: 16/03/2014, 18:20

... Fan C, Katsuyama M, Nishinaka T & Yabe-Nishimura C (2005) Transactivation of the EGF receptor and a PI3 kinase-ATF-1 pathway is involved in the upregulation of NOX1, a catalytic subunit of NADPH ... Suzuki E, Nishimatsu H, Satonaka H, Walsh K, Goto A, Omata M, Fujita T, Nagai R & Hirata Y (2002) Angiotensin II induces myocyte enhancer factor 2- and calcineurin ⁄ n...

Ngày tải lên: 30/03/2014, 03:20

... uncoupling of the Na+-NQR activity by dioxygen Characterization of redox cofactors in membranes from V alginolyticus In the presence of NADH and dioxygen, the purified Na+NQR from V alginolyticus ... the degree of uncoupling of the enzyme The term ÔuncouplingÕ describes the observation that the electrons derived from the oxidation of NADH by the Na...

Ngày tải lên: 31/03/2014, 21:21

... levels of neuronal death, similar to that seen with LPS/IFN-γ treatment alone However, activation of both NADPH oxidase and iNOS was synergistic in inducing neuronal death This neuronal death was ... Shimohama S, Tanino H, Kawakami N, Okamura N, Kodama H, Yamaguchi T, Hayakawa T, Nunomura A, Chiba S, Perry G, Smith MA, Fujimoto S: Activation of NADPH oxidase...

Ngày tải lên: 19/06/2014, 22:20

... Journal of Occupational Medicine and Toxicology 2009, 4:17 Figure by neutrophils in contact with PM2.5 In vitro generation of reactive oxygen and nitrogen species In vitro generation of reactive oxygen ... SEM = Scanning electron microscopy; nd = not detected In vitro Generation of ROS by Neutrophils The in vitro generation of ROS was...

Ngày tải lên: 20/06/2014, 00:20

... changes in biosynthetic activity [15], in addition to apoptosis [16] In addition, ROS can influence transcription factors in chondrocytes and induce the expression of catabolic cytokines [17] ... necessary to investigate the signal transduction pathways of PMA and SIN-1 in chondrocytes Conclusion By amplifying distinct ROS-dependent destructive pathways in cartila...

Ngày tải lên: 09/08/2014, 08:23

... Reactive oxygen species-mediated apoptosis contributes to chemosensitization effect of saikosaponins on cisplatin-induced cytotoxicity in cancer cells Journal of Experimental & Clinical Cancer Research ... suggest that apoptosis is involved in the potentiation of cytotoxicity caused by saikosaponins and cisplatin co-treatment Saikosaponins induce in...

Ngày tải lên: 10/08/2014, 10:20