dym and production of reactive oxygen species ros
Báo cáo khoa học: Antioxidant defences and homeostasis of reactive oxygen species in different human mitochondrial DNA-depleted cell lines pot
Ngày tải lên :
16/03/2014, 18:20
... Determination of intracellular reactive oxygen species as function of cell density Methods Enzymol 352, 91–100 44 Zuo, L & Clanton, T.L (2002) Detection of reactive oxygen and nitrogen species in ... in standard growth conditions anti -ROS buffer in the form of GSH for loss of ROS mitochondrial fluctuation and of ROS spike, occurring when the respiratory chain is active SOD, GST, GPx and catalase ... suggest that increased generation of intracellular ROS in bone q0 cells, relative to muscle q0, is due to increased production of oxidants The high production of ROS in bone-derived q0 cells is...
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Báo cáo khoa học: Muramyl-dipeptide-induced mitochondrial proton leak in macrophages is associated with upregulation of uncoupling protein 2 and the production of reactive oxygen and reactive nitrogen species docx
Ngày tải lên :
05/03/2014, 23:20
... cellular ROS and reactive nitrogen species (RNS) production The increased production of reactive species was apparent after h of stimulation Since UCP2 is described as a regulator of ROS production, ... increase of total NO State Time course effect of MDP on ROS and reactive nitrogen species production by murine peritoneal macrophages + n + /An PI Fig The percentage of viable, dead and apoptotic ... mechanism of action of MDP on the mitochondrial bioenergetics system and since mitochondria are an important source of ROS production and especially of superoxide anion, we investigated the effect of...
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A study to investigate the involvement of nadph oxidase 5 (NOX5) in resveratrol (RSV) induced reactive oxygen species (ROS) production in u937 cells
Ngày tải lên :
26/09/2015, 10:44
... Modulation of cellular redox status 26 (iv) Autophagy and metabolism (v) RSV and Reactive oxygen species (ROS) /NADPH Oxidase (NOX) 1.5: Aim of study 29 1.6: Mode of action of ... dioxide, and small amounts of other gases Air also contains a variable amount of water vapor (an average of 1%) Reactive oxygen species (ROS) are oxygen- derived small molecules (Bedard and Krause, ... of ROS species NOX enzymes are one of the major sources of ROS NOX5 belongs to the family of NOX enzymes NOX5 differs from the other members of the NOX family in aspects as such as its mode of...
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báo cáo khoa học: " Differential patterns of reactive oxygen species and antioxidative mechanisms during atrazine injury and sucrose-induced tolerance in Arabidopsis thaliana plantlets" ppsx
Ngày tải lên :
12/08/2014, 03:20
... suggested important interactions of sucrose and xenobiotic signalling or of sucrose and ROS signalling, thus resulting in induction of specific transcription factors and in an integrated response ... pattern of ROS with increased levels of 1O2 and H2O2 associated with a decrease of O2.content, whereas the protective sucrose-atrazine combination favored accumulation of O2.- and H2O2 These ROS ... xenobiotic and ROS signalling are discussed Results Patterns of accumulation of singlet oxygen, superoxide radical and hydrogen peroxide The transfer of plantlets after weeks of growth to control and...
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Báo cáo y học: " Reduced levels of reactive oxygen species correlate with inhibition of apoptosis, rise in thioredoxin expression and increased bovine leukemia virus proviral loads" potx
Ngày tải lên :
12/08/2014, 23:22
... levels of intracellular reactive oxygen species (ROS) using the 2'-7'-dichlorofluorescein diacetate (CM-H2DCFDA) probe The kinetics of ROS production after short term cultures (30 mn, h and h) ... increased ROS production (Fig 5C) We concluded that VPA-induced apoptosis parallels ROS production To correlate VPA-induced apoptosis in B lymphocytes and reactive oxygen species, ROS production ... mitochondria Confocal microscopy analysis of ROS production in B cells isolated from BLV-infected and control sheep 24 h after culture, PBMCs were labeled with 10 nM of Red CM-XROS probe and stained with...
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Role of neutrophil NADPH oxidase derived reactive oxygen species (ROS) in innate immune responses
Ngày tải lên :
21/05/2016, 22:38
... system What are ROS? ROS include a number of chemically reactive partially reduced forms of oxygen including O2−, H2O2, and HO• These ROS are substantially more reactive than molecular oxygen which ... consisting largely of laminins and collagen type IV, a process which, at least in part is facilitated by leukocyte proteases (Khandoga, Kessler et al 2006) 2.1 Production of ROS of cells of the innate ... fourelectron reduction of O2 to 2H2O and thus ensures the complete reduction of O2 to water without the formation of ROS However, partial reduction of O2, which results in the generation of ROS, can occur...
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báo cáo hóa học:" Airborne particulate matter PM2.5 from Mexico City affects the generation of reactive oxygen species by blood neutrophils from asthmatics: an in vitro approach" docx
Ngày tải lên :
20/06/2014, 00:20
... Medicine and Toxicology 2009, 4:17 Figure by neutrophils in contact with PM2.5 In vitro generation of reactive oxygen and nitrogen species In vitro generation of reactive oxygen and nitrogen species ... the oxidation of tyrosine and nitrite and subsequent formation of tyrosyl and nitrogen dioxide (.NO2) radicals, respectively; these reactive intermediates can initiate the oxidation of lipids in ... respiratory burst and concomitant generation of reactive oxygen species (ROS) , chemical mediators and enzymes in peripheral cells, mainly neutrophils It has been shown that activation of phagocytes...
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Báo cáo khoa học: Structure, regulation and evolution of Nox-family NADPH oxidases that produce reactive oxygen species pptx
Ngày tải lên :
16/03/2014, 06:20
... chain of this residue forming hydrogen bonds with oxygens of the 3-phosphate; NH1 and NH2 of Arg105 form hydrogen bonds with the 4-OH and 5-OH of the inositol moiety; and the aromatic ring of Tyr59 ... regulation and evolution of Nox H Sumimoto Introduction Reactive oxygen species (ROS) are conventionally regarded as inevitable deleterious byproducts of aerobic metabolism On the other hand, there ... precursor of microbicidal ROS The oxidase activity is spatially and temporally restricted to the phagosome, as inappropriate or excessive production of ROS results in damage to surrounding cells and...
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Báo cáo y học: "Reactive oxygen species induce expression of vascular endothelial growth factor in chondrocytes and human articular cartilage explants" pps
Ngày tải lên :
09/08/2014, 08:23
... the nomenclature of free radicals, the term 'reactive oxygen species' (ROS) has prevailed, although ROS can be differentiated into reactive nitrogen species and other oxidant species The relationship ... expression of VEGF, VEGF-121 and VEGF-165 splice variants and VEGF receptors (VEGFR-1 and VEGFR-2) after stimulation by ROS donors Knowledge of this expression could lead to a better understanding of ... released 1,100 pg of VEGF per ml of medium and cartilage explant controls released 540 pg of VEGF per ml of medium Treatment with PMA at concentrations of 5, 10 and 20 μg/ml increased VEGF production...
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Báo cáo y học: " Early The tripeptide feG regulates the production of intracellular reactive oxygen species by neutrophils" pps
Ngày tải lên :
11/08/2014, 08:21
... inhibitor, hispidin, altered the generation of PMA-stimulated ROS in any of the animal groups, indicating an independence of ROS production from PKCα and PKCβ (Figure 3) However, with the naïve ... translocation of the cytosolic components and assembly of the active NADPH oxidase Of particular relevance to PMA-stimulated generation of ROS in neutrophils are the PKC isozymes α, β, and δ These ... inflammation and tissue repair by submandibular gland factors Immunol Today 1994, 15(11):527-532 Rougeot C, Rosinski-Chupin I, Mathison R, Rougeon F: Rodent submandibular gland peptide hormones and other...
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Bone Metabolism: The Role of STAT3 and Reactive Oxygen Species
Ngày tải lên :
24/08/2014, 12:59
... SCHOOL Research Integrity and Copyright Disclaimer Title of Thesis/Dissertation: Bone Metabolism: The Role of STAT3 and Reactive Oxygen Species For the degree of Master of Science Choose your degree ... ROLE OF STAT3 AND REACTIVE OXYGEN SPECIES A Thesis Submitted to the Faculty of Purdue University by America Bethanne Newnum In Partial Fulfillment of the Requirements for the Degree of Master of ... formation and resorption through regulation of nuclear genes, inactivation of STAT3 is shown to disrupt mitochondrial activities and result in an increased level of reactive oxygen species (ROS) Inactivation...
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Báo cáo y học: " Helicobacter pylori induces mitochondrial DNA mutation and reactive oxygen species level in AGS cells"
Ngày tải lên :
25/10/2012, 11:18
... centre of energy metabolism in the cell and a major source of ROS The proportion of oxygen converted into O2•ˉaccounts for about 1-2 % of the overall oxygen consumption [8] Mitochondrial DNA ... mutations is still unknown and the impacts of VacA and CagA on the ROS production and mtDNA mutations are poorly understood To investigate the ROS production and mtDNA mutations in the Hp infected cells, ... function such as the productions of ATP and ROS Elevated ROS level can cause oxidative damage directly to mtDNA resulting in abnormality in ATP production Therefore, the amount of ATP was further...
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Báo cáo khoa học: Neuroglobin and cytoglobin expression in mice Evidence for a correlation with reactive oxygen species scavenging doc
Ngày tải lên :
23/03/2014, 09:21
... dependency and the tissue specificity of Cygb and Ngb mRNA upregulation upon hypoxia and after reoxygenation In addition, we tried to shed light on the ROS- scavenging function of Ngb and on the ... upregulated upon 48 h of hypoxia, and that this increase is dependent on the tissue and the severity of hypoxia The mechanism of induction of Cygb is regulated by HIF-1 The aim of this study was ... role appears to be cellular detoxification of free radicals and related oxidizing species To unravel the possible ROS- scavenging function of Ngb and Cygb, ROS levels, expressed as pmol H2O2 (Fig...
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Báo cáo khoa học: 2-Amino-nonyl-6-methoxyl-tetralin muriate activity against Candida albicans augments endogenous reactive oxygen species production – a microarray analysis study doc
Ngày tải lên :
28/03/2014, 23:20
... 10b Fig Endogenous ROS generation in C albicans SC5314 cells without and with 10b and MCZ The concentrations of 10b and MCZ were 1, and lgÆmL)1 ROS levels represent the mean ± standard deviations ... known antioxidant, and interactions between AA and ROS may attenuate the oxidant effect of ROS and alleviate ROS- induced damage to the organism [33] In this study, addition of AA significantly ... increased ROS production and further damage to mitochondrial components, thus causing a ‘vicious downward spiral’ in terms of ROS production and damage accumulation [23] The antifungal activities of...
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Báo cáo khoa học: Is there more to aging than mitochondrial DNA and reactive oxygen species? pot
Ngày tải lên :
29/03/2014, 22:21
... further increase in ROS production, leading to a ‘vicious cycle’ of ROS production and mtDNA mutations mtDNA + ROS = Aging? l mtDNA mutations, ROS production and cellular damage by ROS eventually ... multiple and redundant ROS scavenging systems mtDNA damage by ROS requires oxidative stress, an imbalance between ROS production and ROS neutralization The mitochondrial pathways for ROS generation and ... nature of respiration, such a leak corresponds to a large quantity of ROS, establishes mitochondrial ETC as a major cellular source of ROS and establishes ROS as compulsory by-products of respiration...
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Báo cáo Y học: Inactivation of the Na+-translocating NADH:ubiquinone oxidoreductase from Vibrio alginolyticus by reactive oxygen species pot
Ngày tải lên :
31/03/2014, 21:21
... cell rupture and preparation of membrane vesicles of V alginolyticus cells were performed under exclusion of dioxygen In addition, the effect of enzymes that detoxify reactive oxygen species (superoxide ... NADH oxidation and Q reduction activities of 2.45 and 0.99 lmolÆmin)1Æmg)1, respectively [15] Inhibition and uncoupling of the Na+-NQR activity by dioxygen Characterization of redox cofactors in ... anaerobic chamber and flushed with N2 In order to investigate the influence of dioxygen and NADH on the activity of the Na+-NQR, membrane vesicles were prepared (A) in the presence of dioxygen without...
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báo cáo hóa học: " Reactive oxygen species drive herpes simplex virus (HSV)-1-induced proinflammatory cytokine production by murine microglia" ppt
Ngày tải lên :
19/06/2014, 22:20
... suppressed ROS production by 11.1%, 18.1%, and 20.9%, at 0.1, 1.0, and 10 μM, respectively Correspondingly, the p38 MAPK inhibitor SB203580 also partially suppressed ROS production by 16.3%, 21.1%, and ... with NAD(P)H oxidase isozyme is essential for lipopolysaccharide-induced production of reactive oxygen species and activation of NF-kappa B J Immunol 2004, 173:3589-3593 Lee IT, Wang SW, Lee CW, ... h p.i., respectively Inhibition of NADPH oxidase blunts virus-induced ROS production We then went on to examine virus-induced ROS production over a time-course of infection In these experiments,...
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báo cáo khoa học: "Reactive oxygen species-mediated apoptosis contributes to chemosensitization effect of saikosaponins on cisplatin-induced cytotoxicity in cancer cells" pdf
Ngày tải lên :
10/08/2014, 10:20
... SA, Lin Y: A critical role of luteolininduced reactive oxygen species in blockage of tumor necrosis factoractivated nuclear factor-kappaB pathway and sensitization of apoptosis in lung cancer ... Generation of reactive oxygen species and induction of apoptosis Journal of carcinogenesis 2009, 8:9 26 Lin Y, Shi R, Wang X, Shen HM: Luteolin, a flavonoid with potential for cancer prevention and ... morphological study of cell death, cells were stained with 50 μg/mL of acridine orange and 50 μg/mL of ethidium bromide and then observed and photographed under a fluorescent microscope Flow cytometry...
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báo cáo khoa học: " Reactive oxygen species and transcript analysis upon excess light treatment in wild-type Arabidopsis thaliana vs a photosensitive mutant lacking zeaxanthin and lutein" pps
Ngày tải lên :
11/08/2014, 11:22
... leading to the over-excitation of pigments and the accumulation of singlet oxygen ( 1O ), a peculiar ROS species that is the first excited electronic state of molecular oxygen [46] We compared wild-type ... accumulation of ROS species and protein oxidizing activity in wild type and npq1lut2 mutant plants Specific probes were used to quantify the accumulation of several ROS in wild type and npq1lut2 ... Crosstalk between singlet oxygen- and hydrogen peroxide-dependent signaling of stress responses in Arabidopsis thaliana Proceedings of the National Academy of Sciences of the United States of...
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Báo cáo khoa học: "A Reactive oxygen species: toxic molecules or spark of life" docx
Ngày tải lên :
12/08/2014, 23:21
... strategies for the manipulation of ROS should take into account the source of ROS, the targets of the ROS, specific cell types involved and the specific location of ROS production in these cells, ... 1/10,000 of extracellular Ca2+ so as to avoid the interaction of Ca2+ and phosphate and bone formation Because of the large transmembrane gradient of Ca2+, the leak of small amounts of Ca2+ across ... paradigm, ROS are not just random destructive species but regulators of metabolic processes and part of the chemistry of life [10,11] Furthermore, evidence of oxidative injury may be the end result of...
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