Baculovirus mediated gene delivery for glioma therapy

... which are suicide gene therapy, genetic immunotherapy, tumor suppressor gene or oncogene approaches, and anti-angiogenesis gene therapy -3- Chapter One: Introduction Suicide gene therapy is one ... Introduction 1.1 Gliomas: the terminator 1.2 Glioma gene therapy: a novel strategy 1.3 Baculovirus: an emerging vector for gene therapy 1.4 Control the gene expression a...

Ngày tải lên: 01/10/2015, 11:39

... with the simplicity and advantageous arrangement of the retroviral genome, which has made retroviruses so attractive as vectors for gene therapy [11,12] The principal feature of the retroviral ... means that the major safety issue faced by those wishing to use retroviral vectors is that of insertional mutagenesis and oncogene activation Insertional mutage...

Ngày tải lên: 14/08/2014, 19:22

... the high turnover, and rapid breeding and maturation time of mice to assess the efficacy of using rAAVmediated gene therapy to replace the missing RPE65 gene in the Rpe65-/- knockout strain The ... reduction in numbers of lipid droplets, the layer of basal infoldings was also thinner in rAAV.RPE65-injected eyes (Fig 6A and 6B) Immunostaining using the anti-SWC opsi...

Ngày tải lên: 14/08/2014, 19:22

... the animals obtained a single intravenous tail vein injection containing various amounts of AAV-MOCS1 in phosphate-buffered saline First, we investigated the effect of a thirty-fold reduced dosage ... AAV-MOCS1 represents a borderline result and indicates a range for the minimal dosage required for abolishing the MoCo deficiency phenotype With a maximum body weight of 40...

Ngày tải lên: 14/08/2014, 19:22

... DEVELOPMENT OF NEW HUMAN STEM CELLDERIVED CELLULAR VEHICLES FOR GLIOMA GENE THERAPY ZHAO YING (B Sc., PKU; M Sc., PKU) A THESIS SUBMITTED FOR THE DEGREE OF DOCTOR OF PHILOSOPHY DEPARTMENT OF ... stem cells by adherent monoculture 4.3.2 “Stemness” of human embryonic stem cell- derived neural 127 stem cells 4.3.3 In vitro glioma tropism evaluation o...

Ngày tải lên: 11/09/2015, 09:00

... GENETIC ENGINEERING AND SURFACE MODIFICATION OF BACULOVIRUS DERIVED VECTORS FOR IMPROVED GENE DELIVERY TO THE CENTRAL NERVOUS SYSTEM YANG YI (B Eng.) A THESIS SUBMITTED FOR THE DEGREE OF DOCTOR ... Chapter Three: Genetic Engineering of Baculovirus Vectors for Controlled Gene Delivery ………65 3.1 Introduction………………………………………………………………… 66 3....

Ngày tải lên: 14/09/2015, 11:28

... astrocytoma grades I, II [astrocytoma], III [anaplastic astrocytoma] and IV [glioblastoma or GM]), oligodendrogliomas, ependymomas and mixed gliomas Gliomas constitute 77% of the primary malignant brain ... the characterization of a glioblastoma specific promoter This study deals with the identification, isolation and characterization of a glioblastoma specific...

Ngày tải lên: 22/10/2015, 21:20

... the surface of PLGA NPs [62] These polymers have been designated as feasible candidates for drug delivery systems, anti -cancer agents and vaccine immunotherapy For example, DNA vaccine delivery ... doi:10.1186/1476-4598-10-3 Cite this article as: Bolhassani et al.: Improvement of different vaccine delivery systems for cancer therapy Molecular Cancer 2011 1...

Ngày tải lên: 06/03/2014, 02:21

... (2004) Physicochemical optimisation of plasmid delivery by cationic lipids J Gene Med 6, S24 S35 15 Wasungu L & Hoekstra D (2006) Cationic lipids, lipoplexes and intracellular delivery of genes ... diameter around lm Use of DOPE and cholesterol to enhance the genedelivery properties of cationic lipids has been extensively documented [1621] For 1,3lb2 and 1,3lb3, tr...

Ngày tải lên: 23/03/2014, 07:20

... al., Fowlpox virus recombinants expressing HPV-16 E6 and E7 oncogenes for the therapy of cervical carcinoma elicit humoral and cell-mediated responses in rabbits Journal of Translational Medicine ... humans Further improvements of the recombinants, using the E6 and E7 transgenes deleted of the p53 and p105Rb cellular binding domai...

Ngày tải lên: 18/06/2014, 16:20

... vAc-Pro427-EGFP D 33kDa 25kDa Figure Baculovirus-mediated promoter analysis of orf427 compared with immediate-early gene ie1 Baculovirus-mediated promoter analysis of orf427 compared with immediate-early ... gene of shrimp white spot syndrome virus Virology 2002, 301:136-147 van Hulten MCW, Westernberg M, Goodall SD, Vlak JM: Identification of two major virion...

Ngày tải lên: 19/06/2014, 08:20

... vAc-Pro427-EGFP D 33kDa 25kDa Figure Baculovirus-mediated promoter analysis of orf427 compared with immediate-early gene ie1 Baculovirus-mediated promoter analysis of orf427 compared with immediate-early ... gene of shrimp white spot syndrome virus Virology 2002, 301:136-147 van Hulten MCW, Westernberg M, Goodall SD, Vlak JM: Identification of two major virion...

Ngày tải lên: 20/06/2014, 04:20

... demonstrated an in vivo targeting of hematopoietic immature cells via a systemic route, mediating an efficient in vivo transgene expression Methods Ab-DNA conjugation The human IL-3 coding sequence ... after in vivo administration [4-10] Tissue-specific targeting can be achieved through ligand receptor interactions [11,12] We have already described a technique of an...

Ngày tải lên: 14/08/2014, 19:22

... a particular gene in the tissue/s of interest The objective of the current study was to identify which one of a group of promoters confers the greatest degree of cardiac specific expression and ... delivery vehicles with well-understood tropisms augment the control investigators have over expression of their transgene of interest and thereby increase the over-all...

Ngày tải lên: 14/08/2014, 19:22