nonviral gene delivery for applications in regenerative medicine

... GAL4 DNA-binding domain with CfEcR ligand-binding domain cloned under the control of the 35S promoter in the pKYLX80 vector A fusion of VP16 activation domain and LmRXR ligand-binding domain cloned ... activity in the presence of ligand when a Kozak sequence containing AACAATGG was used to replace the native luciferase reporter gene Kozak sequence Interestingly, an increase in the ligandinduced ... The performance of the EcR gene switch with the SHILmRXR mutant was compared with that of the gene switches containing wild-type LmRXR and CH9, as a partner of CfEcR, in inducing reporter gene...

Ngày tải lên: 15/03/2014, 23:20

... an essential ingredient for HIV gene therapy In view of the need for CD34 cells for HIV gene therapy as well as for other hematopoietic disorders, if one can produce these cells in unlimited ... control for 30 minutes at 4C before staining with the respective cell surface marker antibodies Isotype control staining was used to determine background levels FACS analysis was performed on ... consistent in replicative experiments The transgene GFP expression was also maintained during the differentiation of hES cells into mature macrophages GFP expression in Page of 11 (page number not for...

Ngày tải lên: 13/08/2014, 09:21

... tissue because they evoke minimal foreign body reactions (Suh and Matthew, 2000) Hence, chitosan has gained considerable interests in regenerative medicine applications, including those associated ... supporting the emergence of regenerative medicine as a forefront field in biomedical technology Biomaterials hold a central role in many tissue regeneration strategies since they provide the platforms ... listed in Table 3.1 Adsorption of growth factor was carried out by immersing a pristine disc in 100 µl of loading solution containing µg/ml of BMP-2 or FGF-2 in PBS for h, followed by a rinsing...

Ngày tải lên: 10/09/2015, 09:23

... factors for many nonviral gene- transfer systems [15,16] The production of protein-based gene- delivery vectors in recombinant form in principle allows their activities to be modied by including heterologous ... containing a minimized CD86 (B72) domain and single-chain antibody fragments for tumor targeting Clin Cancer Res 6, 43144322 25 Proba K, Ge L & Pluckthun A (1995) Functional antibody single-chain ... A cDNA fragment encoding the IBB of human importin-a2 (amino acids 1158), including an additional N-terminal His-tag, was generated by PCR using plasmid pQE70-importin-a (kindly provided by D...

Ngày tải lên: 16/03/2014, 22:20

... years They range from simple monitoring of daily activities, for the purpose of assessing mobility and level of independence in individuals, to integrating miniature sensors to enhance the function ... appropriate for the clinical application of interest; and 3)the development of methodologies to manipulate wearable sensor data to extract information in a clinically relevant manner to perform clinical ... fiber sensors for multi-axis human joint angle measurements", reports encouraging preliminary results concerning monitoring lower limb joint displacements during ambulation by utilizing such technology...

Ngày tải lên: 19/06/2014, 10:20

... spline-based genetic algorithm is applied to matching signals and generating a dense disparity map, from which 3D information is extracted The 1D spline-based genetic algorithm can be executed in ... urgency applications in telemedicine systems The paper entitled “Object delineation by k-connected components” by Paulo Miranda et al develops an image foresting transform for object delineation ... extraction methods including nonparametric weighted feature extraction (NWFE), principal component analysis (PCA), linear discriminant analysis (LDA), combined with different classifiers including k nearest...

Ngày tải lên: 21/06/2014, 22:20

... be transformed into a non-fluorescent state upon initial illumination (termed blinking) This blinking may result in a false negative fluorescence reading during shorter periods of illumination ... permit binding of proteins and nucleic acids that can be used for cellular and intracellular targeting Nanoparticles for Imaging in Fixed Cells New developments in nanoparticle technology in recent ... can be exploited for use in a variety of different applications including cellular imaging and delivery The major types of nanoparticles that have been used for cellular imaging include polymer/biomacromolecular...

Ngày tải lên: 22/06/2014, 18:20

... their surrounding environment to grow and develop into new tissue or organ Indeed, regenerative medicine is an emerging multidisciplinary field involving biology, medicine, and engineering that is ... reduced A and tau pathology in the mouse brain 14 Advances in Regenerative Medicine Conclusions The NCSC is one of the most intriguing cells in the field of regenerative medicine, because it is easily ... need for regenerative medicine to play a more important role in treating such individuals There are two primary approaches to the use of regenerative medicine for patients with cerebral infarction:...

Ngày tải lên: 27/06/2014, 18:20

... their surrounding environment to grow and develop into new tissue or organ Indeed, regenerative medicine is an emerging multidisciplinary field involving biology, medicine, and engineering that is ... reduced A and tau pathology in the mouse brain 14 Advances in Regenerative Medicine Conclusions The NCSC is one of the most intriguing cells in the field of regenerative medicine, because it is easily ... need for regenerative medicine to play a more important role in treating such individuals There are two primary approaches to the use of regenerative medicine for patients with cerebral infarction:...

Ngày tải lên: 28/06/2014, 00:20

... years They range from simple monitoring of daily activities, for the purpose of assessing mobility and level of independence in individuals, to integrating miniature sensors to enhance the function ... appropriate for the clinical application of interest; and 3)the development of methodologies to manipulate wearable sensor data to extract information in a clinically relevant manner to perform clinical ... fiber sensors for multi-axis human joint angle measurements", reports encouraging preliminary results concerning monitoring lower limb joint displacements during ambulation by utilizing such technology...

Ngày tải lên: 11/08/2014, 14:20

... whole of Vietnam has increased from one to two degrees Salt water was going into the land, erratic rain, sun when the flooding, when the prolonged drought The human losses in storms, tsunamis ... the base catalyst samples obtained were survey responses of biomass conversion into organic compounds in the vapor phase Product analysis results, indicating that form a number of organic compounds ... Construction Company, Institute of applied materials Science is promoting technology consultant for several hectares of coconut-water plantation project in Tra Vinh, proposing by Fist-Flower-Companie...

Ngày tải lên: 27/04/2015, 09:10

... Department of Chemistry for their kind help and assistance I appreciate the National University of Singapore for providing me the financial support during the period of this research Finally, I would ... preparation techniques are received increasing attention in the past few decades since they are critical in an analytical procedure Along with the trend in miniaturization in analytical chemistry, microextraction ... when GC is used for the determination step In order to circumvent this problem, a novel sample inlet with a removable insert was developed for the direct injection of IL to GC for the detection...

Ngày tải lên: 09/09/2015, 10:18

... helpful for improving the performance of this cell type in in vitro applications The central role of TGF-β1 and its antagonists in regulating HPTC performance was further confirmed by our findings ... concentration-dependent Following these findings, I generated BMP-7-expressing HPTC by genetic engineering for the development of BMP-7-producing bioartificial kidneys The hypothesis underlying this work was ... cells in the renal cortex in producing or responding to costimulatory cytokines, i.e tumour necrosis factor alpha(35) In addition, PTC produce interleukin-6 in response to inflammatory cytokines...

Ngày tải lên: 09/09/2015, 10:19

... Chapter 1: Introduction   Chapter 1: Introduction The design of biomaterials is important in the field of tissue engineering and regenerative medicine Since the development of tissue engineering scaffolds, ... proteins and new tissue formation Hence, the properties of the scaffolding materials are crucial in determining the success of many tissue engineering applications The general tissue engineering ... that can integrate with the host native tissues 2.2 Biomaterials for Tissue Engineering Biomaterials is one important component in tissue engineering and regenerative medicine and the applications...

Ngày tải lên: 11/09/2015, 09:10

... proteins were retained in this manner indicating minimal processing damage Investigations into the performance of this novel hybrid mesh as a three-dimensional (3D), bio-functional tissue engineering ... structure were investigated via histological staining (Hematoxylin & Eosin) Cellular infiltration was not observed in conventional PCL/Col and PCL/Col-gel groups Marginal infiltration was seen in the ... tissue engineering emerged with the ultimate goal to develop biological substitutes that restore, maintain or improve tissue function [3] By combining life sciences, engineering principles and innovation,...

Ngày tải lên: 14/09/2015, 08:24

... developed membranes were examined for their properties and performance in heavy metal ion removal for environmental applications and in enzyme immobilization for bioengineering perspectives 1.2 Objectives ... solution (dope) before phase inversion c increasing the polymer concentration in the casting solution d applying an evaporation step before the immersion into the coagulation bath e adding solvent ... with chitosan in this study Some applications in environmental engineering or bioengineering with the prepared CS-based hollow fiber membranes will be demonstrated Therefore, efforts will be...

Ngày tải lên: 14/09/2015, 08:49

... 1.7 ml/min The following temperature program was employed: initial temperature of 60 °C for min; increased at 10 °C/min to 260 °C, held for The injector temperature was 270 °C and all injections ... sorbent on the SPME fiber Generally, SPME is a fine sample preparation technique: simple, convenient, generally fast and solvent free (for GC applications) Therefore, since its introduction, SPME has ... thicker coating However, the main drawback of this technique is that the introduction and desorption of extracting membrane in the GC injection liner is quite complicated PC-HFME was developed in our...

Ngày tải lên: 14/09/2015, 11:45

... out in an Eppendorf® thermal cycle using the following program: 50°C for 30 minutes and 94°C for minutes, followed by 38 cycles of 94°C for 15 sec, 58°C for 30 sec, and 72°C for minutes, and final ... available imaging system for BLI is more affordable than the expensive instruments for MRI and PET Therefore, BLI has been increasingly applied in preclinical animal studies - 19 - Chapter One: Introduction ... 37ºC in a humidified incubator with 100% air Insect Sf9 cell line purchased from Invitrogen was cultured in Sf-900 II SFM medium with penicillin streptomycin (0.5%) at 27ºC in a non-humidified incubator...

Ngày tải lên: 01/10/2015, 11:39

... Phenylalanine G Gly Glycine H His Histidine I Ile Isoleucine K Lys Lysine L Leu Leucine M Met Methionine N Asn Asparagine P Pro Proline Q Gln Glutamine R Arg Arginine S Ser Serine T Thr Threonine V ... of the serine protease thrombin by examining its preferences for individual peptides on a miniaturized fluorogenic assay This had the potential of generating a proteolytic fingerprint of each ... compatibility in both in vivo and in vitro settings, as well as their suitability for both quantitative analyses for real-time monitoring of enzyme kinetics and for visual tracking of enzymatic...

Ngày tải lên: 04/10/2015, 15:45

... system, including the cerebral cortex, the brain Chaapter Introoduction stem m, the gastrrointestinal tract, and the t pancreaas It plays many diverse roles inncluding inhiibition of enndocrine annd ... disulfide in a native protein, the two free thiol groups are ready for the site-specific intercalation (in case the protein offers just a single accessible disulfide bridge) Generally, the intercalating ... [53] 1.5 Site-specific Intercalation into Proteins using a Three-Carbon Bridge 1.5.1 Disulfide Bonds in Therapeutically Relevant Proteins In general, free and accessible cysteine residues are rare[54]...

Ngày tải lên: 16/10/2015, 11:57