needle free particle mediated gene delivery
Báo cáo sinh học: " Recombinant adeno-associated virus type 2-mediated gene delivery into the Rpe65-/- knockout mouse eye results in limited rescue" ppsx
Ngày tải lên :
14/08/2014, 19:22
... degeneration slow mice by gene therapy Nat Genet 2000, 25:306-310 Sarra GM, Stephens C, de Alwis M, Bainbridge JW, Smith AJ, Thrasher AJ, Ali RR: Gene replacement therapy in the retinal degeneration ... vivo The efficiency of transgene delivery would be particularly important in diseases characterized by pan-retinal degeneration as they would presumably require the rAAV -mediated treatment to be ... photoreceptor degeneration, as well as optimizing the timing, efficiency and specificity of the current rAAV gene technology Although gene therapy on the Rpe65-/- mouse model may not have generated...
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Báo cáo sinh học: "The use of retroviral vectors for gene therapy-what are the risks? A review of retroviral pathogenesis and its relevance to retroviral vector-mediated gene delivery" pptx
Ngày tải lên :
14/08/2014, 19:22
... retroviral genes Oncogene capture The mechanism of oncogene capture appears to be dependent on the generation of a chimeric retroviraloncogene transcript (69, 70) This suggests that the risk of oncogene ... retroviral vectors is that of insertional mutagenesis and oncogene activation Insertional mutagenesis and oncogene activation As discussed above, oncogene activation can occur either by transcription ... and insertional mutagenesis [71,72] are the two risk factors of significance in retroviral mediated gene therapy The two known instances where insertional mutagenesis/ oncogene activation has...
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Baculovirus mediated gene delivery for glioma therapy
Ngày tải lên :
01/10/2015, 11:39
... which are suicide gene therapy, genetic immunotherapy, tumor suppressor gene or oncogene approaches, and anti-angiogenesis gene therapy -3- Chapter One: Introduction Suicide gene therapy is one ... for gene therapy It is impossible to obtain success in gene therapy without effective gene delivery systems that can achieve high levels of therapeutic gene expression in targeted cells Gene delivery ... Baculovirus -mediated gene silencing effects in vitro Fig 16 Quantitative analyses of baculovirus -mediated gene silencing effects in C6 cells -V- Fig 17 Quantitative analyses of baculovirus -mediated gene...
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Báo cáo sinh học: "In vivo gene targeting of IL-3 into immature hematopoietic cells through CD117 receptor mediated antibody gene delivery" pot
Ngày tải lên :
14/08/2014, 19:22
... complexes in gene delivery: from biophysics to biological applications Advanced Drug Delivery Reviews 2001, 47:277-294 Cristano RJ, Curiel DT: Strategies to gene delivery via the receptor -mediated ... Huang L: Protamine sulfate enhance lipid mediated gene transfer Gene therapy 1997, 4:961-968 Gao X, Huang L: Potentiation of cationic liposome mediated gene delivery by polycations Biochemistry 1996, ... cells Conclusions Our gene delivery system is specific and leads to transient gene delivery and expression It may prove useful and safe for numerous clinical applications of gene transfer in hemato-oncology...
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Báo cáo sinh học: "Tissue specific promoters improve specificity of AAV9 mediated transgene expression following intra-vascular gene delivery in neonatal mice" pptx
Ngày tải lên :
14/08/2014, 19:22
... mediate long-term transgene expression in muscle Hum Gene Ther 1997, 8:659-669 Kessler PD, Podsakoff GM, Chen X, McQuiston SA, Colosi PC, Matelis LA, Kurtzman GJ, Byrne BJ: Gene delivery to skeletal ... characterization of different promoters and their respective behavior in vivo following AAV9 mediated gene delivery Our data indicate that the α-MHC promoter confers the most cardiac specific expression ... delivery vehicles with well-understood tropisms augment the control investigators have over expression of their transgene of interest and thereby increase the over-all safety of therapeutic gene...
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Báo cáo khoa học: Recombinant derivatives of the human high-mobility group protein HMGB2 mediate efficient nonviral gene delivery pptx
Ngày tải lên :
16/03/2014, 22:20
... SV40NLS-HMGB2186 -mediated gene delivery into COS-7 and HepG2 cells were very similar to those obtained after TATPTD-HMGB2186 -mediated transfection at comparable molar protein to DNA ratios For gene delivery ... unidentied target molecule HMGB2186 facilitates gene delivery into COS-7 cells To investigate HMGB2186 -mediated gene transfer, the pCMS-eGFP-E2C-Luc reporter gene plasmid was used which encodes enhanced ... in Fig 4B,C,E, in all three cell lines not only HMGB218 6mediated and SV40NLS-HMGB2186 -mediated, but also TATPTD-HMGB2186 -mediated gene delivery was enhanced by chloroquine to a similar degree...
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Báo cáo khoa học: K182G substitution in DevR or C8G mutation in the Dev box impairs protein–DNA interaction and abrogates DevR-mediated gene induction in Mycobacterium tuberculosis doc
Ngày tải lên :
22/03/2014, 16:20
... valuable insights into the genetic programmes utilized by M tuberculosis during dormancy adaptation [4] Transcription represents the first and the most crucial step in gene regulation in prokaryotes ... abrogation of interactions mediated by a single amino acid, namely Lys182, with the primary binding site is alone sufficient to abolish specific DNA–protein interactions and downstream gene induction Results ... of WT DevR protein; lane 10 represents free DNA; lanes 11 and 12 contain 0.5 and 1.0 lM of K182G mutant DevR protein (C) qRT-PCR analysis of DevR regulon gene expression RNA was isolated from...
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Báo cáo khoa học: NovelN,N¢-diacyl-1,3-diaminopropyl-2-carbamoyl bivalent cationic lipids for gene delivery – synthesis,in vitro transfection activity, and physicochemical characterization docx
Ngày tải lên :
23/03/2014, 07:20
... at + ) : 1, generated a particle size of the smallest mean diameter In serumfree medium, liposome and lipoplex particle sizes for all lipids generally increased, and the overall particle size ... their gene- delivery FEBS Journal 275 (2008) 148162 ê 2007 The Authors Journal compilation ê 2007 FEBS 157 Novel cytofectins for gene delivery M Spelios and M Savva capabilities Lipofection mediated ... lipid) -mediated in vivo gene delivery to mouse liver Gene Ther 10, 180187 20 Narang AS, Thoma L, Miller DD & Mahato RI (2005) Cationic lipids with increased DNA binding afnity for nonviral gene...
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gene delivery to mammalian cells, volume 1
Ngày tải lên :
10/04/2014, 22:42
... mechanisms underlying synthetic vector -mediated DNA delivery With the continuous effort to meet the need for safe and efficient gene- delivery methods for human gene therapy, it is foreseeable that ... sharing unpublished information on PNA -mediated gene transfer Chemical Methods for DNA Delivery 19 References Wu, G Y and Wu, C H (1987) Receptor -mediated gene transformation by a soluble DNA ... peptide-based gene delivery systems for use in plasmid-based gene therapy Biophys J 74, 2802–2814 14 Mahat, R I., Monera, O D., Smith, L C., and Rolland, A (1999) Peptide-based gene delivery Curr...
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gene delivery to mammalian cells, volume 2
Ngày tải lên :
11/04/2014, 00:48
... to generate an adenoviral vector capable of efficient gene delivery to mature skeletal muscle upon systemic administration In this chapter, we describe methods for adenovirus -mediated gene delivery ... Press, Totowa, NJ, pp 1–9 Adenovirus -Mediated Gene Delivery to Skeletal Muscle Joanne T Douglas Introduction Adenoviral vectors can be employed for gene delivery to skeletal muscle, both ex vivo ... adenovirus -mediated gene transfer to the muscle suffers from the unique problem of a matu- From: Methods in Molecular Biology, vol 246: Gene Delivery to Mammalian Cells: Vol 2: Viral Gene Transfer...
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báo cáo hóa học:" Microarray analysis of Foxl2 mediated gene regulation in the mouse ovary derived KK1 granulosa cell line: Over-expression of Foxl2 leads to activation of the gonadotropin releasing hormone receptor gene promoter" pptx
Ngày tải lên :
20/06/2014, 07:20
... lists 22 common genes Sheet lists 29 common genes Sheet lists 21 common genes Sheet lists 155 genes unique to KK1 & E16 Sheet lists 177 genes unique to KK1 & E13 Sheet 10 lists 270 genes unique ... expressed genes in KK1 cells to E13, E16, and P0 Sheet lists 225 genes common to KK1 and E13 Sheet lists 211 genes common to KK1 and E16 Sheet lists 325 genes common to KK1 and P0 Sheet lists genes ... follows: E13, 3289 genes (Additional file 6); E16, 2995 genes (Additional file 7); and P0, 4330 genes (Additional file 8) Comparisons between groups of genes were performed with Genesifter Intersector...
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Báo cáo y học: "Adverse effects of adenovirus-mediated gene transfer of human transforming growth factor beta 1 into rabbit knees" potx
Ngày tải lên :
09/08/2014, 01:21
... McCormack JE, Evans CH, Robbins PD: Direct retrovirus -mediated gene transfer to the synovium of the rabbit knee: implications for arthritis gene therapy Gene Ther 1997, 4:977-982 17 Mi Z, Ghivizzani ... Figure Adenovirus -mediated transforming growth factor (TGF)-β1 gene expression in naïve and antigen-induced arthritis (AIA) rabbit joints (A) × 107, × 108 and × 109 adenoviral particles encoding ... (B) × 109 viral particles were injected into naïve rabbit left knees (C) × 108 and × 109 viral particles were injected into AIA rabbit left knees The same amounts of control viral particles were...
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Báo cáo y học: "Human, viral or mutant human IL-10 expressed after local adenovirus-mediated gene transfer are equally effective in ameliorating disease pathology in a rabbit knee model of antigen-induced arthritis" ppt
Ngày tải lên :
09/08/2014, 08:22
... direct viral gene transfer of therapeutic agents provide proof supporting the use of gene therapy in arthritis [9-13,32,33], and a recent phase I clinical trial using IL-1Ra by ex vivo gene transfer ... successfully completed [40,41] Systemic non-viral delivery methods, such as administration of engineered syngeneic fibroblasts [42], and intranasal delivery of plasmid DNA [43] also show promise ... considerable potential for development of clinical gene therapy approaches for RA Conclusion In this report, we demonstrate by adenoviral -mediated intraarticular gene transfer to the rabbit knee that hIL-10,...
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Báo cáo y học: " Substitution of the Rev-response element in an HIV-1-based gene delivery system with that of SIVmac239 allows efficient delivery of Rev M10 into T-lymphocytes" ppsx
Ngày tải lên :
10/08/2014, 05:21
... Research and Therapy 2008, 5:11 or gene- transfer vector constructs or target host genes, such as the gene for the CCR5 coreceptor [3-5] All lentivirus-based gene delivery systems contain packaging ... hybrid genes without the use of restriction enzymes: gene splicing by overlap extension Gene 1989, 77:61-68 Horton RM, Cai ZL, Ho SN, Pease LR: Gene splicing by overlap extension: tailor-made genes ... constitutive transport element Gene Ther 2000, 7:1421-1424 Corbeau P, Kraus G, Wong-Staal F: Transduction of human macrophages using a stable HIV-1/HIV-2-derived gene delivery system Gene Ther 1998, 5:99-104...
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Characterization of Fabry mice treated with recombinant adeno-associated virus 2/8-mediated gene transfer pdf
Ngày tải lên :
10/08/2014, 05:21
... regarding a comparison study of gene therapy and ERT In the present study, we investigated pseudotyped rAAV2/8 -mediated gene delivery of α-Gal A and compared the efficacy of gene therapy with that of ... enzyme necessary for therapy Therefore, gene therapy for Fabry disease has been explored using a variety of viral vector delivery systems [10-13] These gene therapy studies appear to be effective ... heart, spleen, and brain of Fabry mice injected with × 1012 particles of rAAV 2/2-hAGA, × 1011 particles of rAAV 2/8-hAGA, or × 1012 particles of each rAAV 2/8-hAGA vector The genomic dosage of...
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Sonoporation-mediated gene transfer into adult rat dorsal root ganglion cells docx
Ngày tải lên :
10/08/2014, 05:21
... higher potential for clinical application, even although their efficacy for gene delivery is lower [34] Liposome -mediated gene transfer involves the fusion of synthetic lipids into the plasmid membrane, ... Hwang LH, Chen WS: Differences in gene expression between sonoporation in tumor and in muscle J Gene Med 2009 Sakai T, Kawaguchi M, Kosuge Y: siRNA -mediated gene silencing in the salivary gland ... Sonoporation: mechanical DNA delivery by ultrasonic cavitation Somat Cell Mol Genet 2002, 27:115-134 Fischer AJ, Stanke JJ, Omar G, Askwith CC, Burry RW: Ultrasoundmediated gene transfer into neuronal...
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báo cáo khoa học: "ShRNA-mediated gene silencing of MTA1 influenced on protein expression of ER alpha, MMP-9, CyclinD1 and invasiveness, proliferation in breast cancer cell lines MDA-MB-231 and MCF-7 in vitro" pptx
Ngày tải lên :
10/08/2014, 10:21
... http://www.jeccr.com/content/30/1/60 the gene therapy is the research focus on prevention and treatment of tumor Currently, gene therapies for tumor include gene replacement, antisense nucleic acid technique, cytokine gene therapy, ... profiles of target genes A number of genes, including cyclinD1, are regulated by ER alpha[7] In this study, two shRNA plasmid vectors against MTA1, which could persistently generate siRNA inside ... MTA1 According to principle of shRNA, enzyme inciding site of vector pGenesil-1 and exon of MTA1 (GeneBank, No NM004689) in GeneBank, two target DNA fragments were designed and constructed to coding...
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báo cáo khoa học: " Effects of RNA interference-mediated gene silencing of JMJD2A on human breast cancer cell line MDA-MB-231 in vitro" pptx
Ngày tải lên :
10/08/2014, 10:21
... the tumor suppressor gene ARHI in breast cancer Oncogene 2006, 25:230-239 doi:10.1186/1756-9966-30-90 Cite this article as: Li et al.: Effects of RNA interference -mediated gene silencing of JMJD2A ... Mol Cell 2005, 19:381-391 26 Gomes NP, Espinosa JM: Gene- specific repression of the p53 target gene PUMA via intragenic CTCF-Cohesin binding Genes Dev 2010, 24:1022-1034 27 Kawazu M, Saso K, Tong ... reports also link deregulated histone methylation to tumorigenesis [15,16] An H3K9 histone methyltransferase, Suv39H1, Silencing JMJD2A gene suppressed MDA-MB-231 cell migration and invasion in...
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Báo cáo y học: " Pathogenic effect of interleukin-17A in induction of Sjögren’s syndrome-like disease using adenovirus-mediated gene transfer" docx
Ngày tải lên :
12/08/2014, 15:22
... JA, Baum BJ: Local adeno-associated virus -mediated interleukin 10 gene transfer has disease-modifying effects in a murine model of Sjogren’s syndrome Hum Gene Ther 2003, 14:1605-1618 27 Kok MR, ... 32:414-421 Kagami H, O’Connell BC, Baum BJ: Evidence for the systemic delivery of a transgene product from salivary glands Hum Gene Ther 1996, 7:2177-2184 Wang J, Voutetakis A, Mineshiba F, Illei ... serotype adenoviral and serotype adeno- associated viral vector -mediated gene transfer to salivary glands on the composition of saliva Hum Gene Ther 2006, 17:455-463 Adesanya MR, Redman RS, Baum BJ,...
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Báo cáo y học: " Toward more effective gene delivery" docx
Ngày tải lên :
14/08/2014, 08:20
... aimed at managing the immunological challenges confronting systemic delivery of therapeutic viruses Vector neutralization Viral gene- delivery vectors, in particular those administered intravenously, ... lipoprotein lipase transgene, which was correlated with the loss of therapeutic efficacy Such immune stimulation was not induced in response to transgene-derived antigens, but was generated specifically ... maintained episomally to achieve sustained therapeutic gene expression An emerging problem preventing the persistence of AAVintroduced genes is a cytotoxic CD8 T-cell response that is elicited...
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