liposome protamine dna complexes for gene delivery

... cytofectins for gene delivery H3C H3C N CH3 N H3C N O O O NH O NH R R The R group varies with the derivative: C11H23 for dilauroyl (1,3lb1) C13H27 for dimyristoyl (1,3lb2) C15H31 for dipalmitoyl ... lipid)-mediated in vivo gene delivery to mouse liver Gene Ther 10, 180187 20 Narang AS, Thoma L, Miller DD & Mahato RI (2005) Cationic lipids with increased DNA binding afnity for nonviral gene transfer ... AJ, Samuel CE & Sanya CR (2004) Cationic lipidDNA complexes for gene therapy: understanding the relationship between complex structure and gene delivery pathways at the molecular level Curr Med...

Ngày tải lên: 23/03/2014, 07:20

... than DNA alone for luciferase gene delivery (500-600 times more efficient) A dose response curve for gene delivery of six different NP /DNA complexes to 293 cells has been generated; optimal delivery ... DNA exhibited a high transfection efficiency (99%) and 100% cell viability Study of gene delivery with hybrid nanoparticle /DNA complexes using other cell lines The optimal condition for DNA gene ... Degradation of NP /DNA complexes delivered to 293 cells For these experiments, we freshly prepared the six NP/ DNA complexes, using a NP /DNA (w/w) ratio of 40/1 (Figure 1) Such complexes bound DNA at a...

Ngày tải lên: 11/08/2014, 00:22

... retroviral genes Oncogene capture The mechanism of oncogene capture appears to be dependent on the generation of a chimeric retroviraloncogene transcript (69, 70) This suggests that the risk of oncogene ... in activation of an oncogene, in combination with the requirement for other genetic changes before a cancer eventuates Although proviral integration can also result in gene inactivation, inactivation ... retroviral vectors is that of insertional mutagenesis and oncogene activation Insertional mutagenesis and oncogene activation As discussed above, oncogene activation can occur either by transcription...

Ngày tải lên: 14/08/2014, 19:22

... contaminating plasmid DNA was removed by treatment with DNAse I, amplification-grade (Invitrogen) The cDNA (2 µg) was amplified for 35 cycles at 94°C for 30 seconds, 60°C for 45 seconds and 72°C for 1.5 minutes, ... methods Plasmid DNA construction and purification The construction of a pcDNA3 plasmid containing the cytomegalovirus (CMV) promoter and a cDNA encoding the HSP65 gene for M leprae (pcDNA3-HSP65) ... Center for Biotechnology Information, Bethesda, MD, USA) Transformation of E coli with µg of wild type pcDNA3-hsp65 was used as positive control of transformation Methylation status of plasmid DNA...

Ngày tải lên: 14/08/2014, 19:22

... intervals); DNA- hsp65 (three intramuscular injection of 100 ug DNA plasmid encoding M leprae hsp65 gene at 2-week intervals); DNAv (plasmid DNA without the hsp65 gene administered at the same scheme for ... animals vaccinated and challenged with M tuberculosis Formulationsa Miceb Guine-pigsb No vaccination BCG DNAv (naked DNA) DNAhsp65 (naked DNA) Me-DNAhsp65/TDM Me-Prime/boost 74 ± 18 ± 5* 72 ± 38 ± ... Encapsulation efficiency varied from 30 to 50% for DNA and around 70% for protein Table shows the amount of entrapped DNA and protein in the different formulations In this work, particles were designed...

Ngày tải lên: 14/08/2014, 19:22

... chosen for the gene delivery experiment High molecular weight bPEI up to 800 kDa has been used for non-viral gene transfer, exhibiting a superior capability to form compact and stable bPEI /DNA complexes ... of complexes formed by either cationic lipids or cationic polymers, which are able to protect DNA from nuclease degradation and deliver them to the cells However, complexes formed by cationic liposomes, ... are typically very efficient in gene transfer They can be transformed into effective gene delivery systems by replacing part of their genome with a therapeutic gene However, safety concerns remain...

Ngày tải lên: 10/09/2015, 09:05

... improved gene delivery could be achieved by using these vectors In particular, gene delivery vectors evaluated in this study would be most useful for gene therapy for CNS diseases Nevertheless, the generalizable ... vectors for gene delivery to the nervous system has been limited by their ability to transfer genes only to dividing cells, although they are well suited for on-site delivery to neural precursors for ... Particles…………141 5.1.2 Genetic Engineering of Baculovirus Vectors for Controlled Gene Expression……………………………………………………… 142 5.1.3 Surface Modifications of Baculovirus Vectors for Improved In Vivo Gene Delivery ………………………………………………...

Ngày tải lên: 14/09/2015, 11:28

... 15 Chapter Introduction generated for gene delivery, the Rep and Cap genes are replaced by the therapeutic genes AAV is a commonly used viral vector, especially for gene delivery to the CNS, mainly ... 1.4 Vectors used for gene delivery to the CNS Three types of gene delivery methods, namely physical methods, non-viral methods and viral methods, have been applied for gene delivery to mammalian ... used for gene therapy applications, and were rarely used for 12 Chapter Introduction gene delivery to the CNS because of their poor in vivo performance Non-viral and viral vectors are therefore...

Ngày tải lên: 16/09/2015, 08:30

... efficient delivery for most drugs (Castro et al., 2003a) 1.3.2 Gene therapy of gliomas One of the formidable tasks of intracranial gene delivery is the difficulty in achieving gene delivery to ... RNase A (DNase Free) 2.1.9 Propidium iodide (PI) solution 2.1.10 Sucrose solution 2.1.11 Solution I for DNA extraction 2.1.12 Lysis solution for DNA extraction 2.1.13 Neutralizing buffer for DNA extraction ... enhanced angiogenesis in tumor progression (Albertoni et al., 1998; Ravi et al., 1998) Other promising novel therapeutic genes for glioma therapy include genes that target angiogenenic factors...

Ngày tải lên: 16/09/2015, 15:54

... which are suicide gene therapy, genetic immunotherapy, tumor suppressor gene or oncogene approaches, and anti-angiogenesis gene therapy -3- Chapter One: Introduction Suicide gene therapy is one ... vector for gene therapy It is impossible to obtain success in gene therapy without effective gene delivery systems that can achieve high levels of therapeutic gene expression in targeted cells Gene ... following program: 50°C for 30 minutes and 94°C for minutes, followed by 38 cycles of 94°C for 15 sec, 58°C for 30 sec, and 72°C for minutes, and final extension at 72°C for 10 minutes For the detection...

Ngày tải lên: 01/10/2015, 11:39

... Test Cord Used for testing HIGHBAND 10 circuits Product Number: 6451 100-30 3M Pack Qty: Bag of Please contact KRONE for further information on the full range of Test/Patch cords for the HIGHBAND ... SERIES “Supporting telecommunication networks throughout the world” “The Next Generation of Connectivity” for Broadband Delivery Individual pairs closer together Adjacent pairs higher seperation ... telecommunications suppliers to lay the foundation for public access communications infrastructure on both copper and fibre optic cabling architectures Enabling the delivery of both today’s and tomorrows...

Ngày tải lên: 09/12/2013, 15:15

... central office/CBD Customer Delivery Transport Delivery Customer • Broadband Delivery Solutions, ie local loop • Subscriber Interface Solutions KRONE : Your Partner for Quality and the Finest ... Internet Access or combinations of these and other services, the ‘Speed for Need’ is critical To satisfy this need for speed the delivery infrastructure has to be resilient, cost effective and bandwidth ... SUBSCRIBER INTERFACE SOLUTIONS Products for both residential and commercial subscribers that solve issues around Network Termination Points, receiving and managing information via POTS, ISDN, xDSL, etc...

Ngày tải lên: 22/12/2013, 12:15

... presence of hetero ⁄ homo- duplex DNA MutS -DNA complexes were formed by incubating 0.15 lM of MutS with either heteroduplex (n) or homoduplex (s) DNA (0.15 lM each) for 10 at 22 °C in buffer containing ... mismatch in the DNA on the Fig ATP induced aggregation of MutS-MutL in presence of homo ⁄ heteroduplex DNA MutS-MutL -DNA complexes were formed by incubating of MutS-MutL (preincubated for by mixing ... controls and annealing was achieved with > 90% of efficiency MutS DNA complex formation MutS DNA complexes are formed by adding duplex DNA (homo- or heteroduplex) to MutS in buffer (50 mm Hepes pH...

Ngày tải lên: 07/03/2014, 12:20

... condense DNA is essential for a nonviral vector to be successful as a gene- delivery reagent [4] Although synthetic vectors are most commonly used for nonviral gene transfer, certain DNA- condensing ... SV40NLS-HMGB2186-mediated gene delivery into COS-7 and HepG2 cells were very similar to those obtained after TATPTD-HMGB2186-mediated transfection at comparable molar protein to DNA ratios For gene delivery into ... unmodied HMGB2186 protein before IBB-HMGB218 6DNA complexes at an optimal protein to DNA mass ratio of 8.2 were added Control cells were treated with IBB-HMGB218 6DNA complexes in the absence of...

Ngày tải lên: 16/03/2014, 22:20

... underlying synthetic vector-mediated DNA delivery With the continuous effort to meet the need for safe and efficient gene- delivery methods for human gene therapy, it is foreseeable that significant advances ... sequences, and for gene therapy The process of introducing DNA into cells for the purpose of gene expression is called transfection or gene delivery Synthetic compounds used to facilitate DNA transfer ... further improvement in gene- delivery efficiency for human gene therapy In attempting to predict what surprises the next 5–10 years might hold for chemical methods for DNA delivery, we believe that...

Ngày tải lên: 10/04/2014, 22:42

... subsequent generations of Ad vectors have been designed to be defective for multiple viral genes, in addition to E1 The removal of genes encoding proteins essential for DNA replication (the DNA binding ... dystrophin gene together with regulatory sequences, and are therefore appropriate vehicles for gene- replacement therapy for Duchenne muscular dystrophy In addition to their suitability for in vivo gene- therapy ... been used for cloning and delivery of single genes Here we propose the use of AdVs for generating libraries by positive selection of recombinants When transient expression is sufficient for functional...

Ngày tải lên: 11/04/2014, 00:48

... vectors for gene therapy formed by self-assembly of DNA with synthetic block co-polymers Hum Gene Ther 7, 2123–2133 11 Kabanov, A V and Kabanov, V A (1995) DNA complexes with polycations for the delivery ... Acid Side Chains for DNA Carrier Atsushi Maruyama and Yoshiyuki Takei Introduction Polycations have been used as nonviral gene carriers because the polycations and DNAs form stable complexes in a ... (1999) A novel gene delivery system for genetic manipulation of sinusoidal )endothelial cells by triplex DNA technology: evaluation of targetability and ability to stabilize triplex formation Hepatology...

Ngày tải lên: 11/04/2014, 09:59

... unknown, and therefore must be predicted from reported sequences of the same gene m different orgamsms or from sequence mformation from similar genes For structural genes, the known DNA sequence homologs ... incubator for 3-5 h to allow the colonies to regrow (see Note 4) Direct Cloning of cDNA Boil the colonies for mm Microfuge at 16,000g for Use JJL of the supernatant as template for the PCR For each ... to become transformed (6) For purposes where a high efficiency but a low frequency of transformation is required (for library construction where cotransformants are undesirable) a DNA concentration...

Ngày tải lên: 11/04/2014, 10:14

... Type 1-Based Vectors for Gene Delivery to Human Hematopoietic Stem Cells Ali Ramezani and Robert G Hawley 467 Semliki Forest Viral Vectors for Gene Transfer Jarmo Wahlfors and Richard A Morgan ... Krisky, D., Oligino, T., et al (2000) Genetically modified CD34+ cells as cellular vehicles for gene delivery into areas of angiogenesis in a rhesus model Gene Ther 7, 43–52 140 Laquerre, S., Anderson, ... classes of genes; the IE or α genes, the early (E) or β genes, and the late (L) or γ genes (2) There are five IE genes; infected cell polypeptides (ICP) 0, 4, 22, 27, and 47 Four of these IE genes...

Ngày tải lên: 11/04/2014, 10:39

... of drug delivery, the requirements for nanoparticulate systems can vary These aspects are discussed in Chapter for injectable delivery, Chapter for oral delivery, Chapter 10 for brain delivery, ... Chapter 11 for ocular delivery, and Chapter 12 for gene delivery Finally, the fourth section provides an overview of the clinical, ethical, and regulatory issues of nanoparticle-based drug delivery ... Supercritical Solution for Particle Formation 59 RESS with Solid Cosolvent for Nanoparticle Formation 63 Supercritical Antisolvent Process for Particle Formation 66 SAS with Enhanced...

Ngày tải lên: 04/06/2014, 15:15