device mediated gene delivery a review

... administered to animals viral replication per se did not appear to have an overt pathogenetic affect, rather a T-cell lymphoma eventuated [62], presumably as a result of oncogene activation Although ... vectors is that of insertional mutagenesis and oncogene activation Insertional mutagenesis and oncogene activation As discussed above, oncogene activation can occur either by transcription from ... play Clinical trials are based on extensive preclinical experimentation and animal trials that take many years to complete Clearly, the particular vector system that has been used to develop a...

Ngày tải lên: 14/08/2014, 19:22

... WY, Tabarias H, Constable I, Kanagasingam Y, Barry CJ, Rakoczy PE: Evaluation of adeno-associated virus -mediated gene transfer into the rat retina by clinical fluorescence photography Hum Gene ... conceptual design of the project, initiated the collaborations and assisted in data analysis All authors have had intellectual contribution to the preparation of this manuscript and have read and approved ... Hereditary Characteristics Veterinary & Comparative Ophthalmology 1994, 4:85-92 Acland GM, Aguirre GD, Ray J, Zhang Q, Aleman TS, Cideciyan AV, Pearce-Kelling SE, Anand V, Zeng Y, Maguire AM, Jacobson...

Ngày tải lên: 14/08/2014, 19:22

... overlay onto a gray scale animal image Images and measurements of luminescent signals were acquired and analyzed using the Living Image software (Xenogen, Alameda, California, USA) Animals were ... detection and analysis Gene specific primers for the DTA gene were used, Forward primer: 5’-AAATACGACGCTGCGGGAT-3’, Reverse primer 5’-GAAGGGAAGGCTGAGCACTA-3’ RT-PCR reaction was carried out in an Eppendorf® ... (5’-GCTTACGCTGAGTACTTCGATTCAAGAGATCGAAGTACTCAGCGTAAG - 25 - Chapter Two: Materials and Methods CTTTTT-3’) targeting against the firefly (Photinus pyralis) luciferase coding region with cohesive BamH...

Ngày tải lên: 01/10/2015, 11:39

... from media was chromatographically separated using PD-10 columns (Phar- macia, Piscataway, NJ, USA), and radiolabeled GAGs released into the culture media or recovered from the alkaline treatment ... rabbit knee (b) Synovium from an AIA rabbit knee that received Ad.eGFP as an inflamed control (c) Synovium from an AIA rabbit knee that was treated with Ad.hIL-10 (d) Synovium from an AIA rabbit ... The animals were acclimatized for three days before experimentation and were fed chow ad libitum and water All animal experiments were conducted in accordance with NIH standards of animal care and...

Ngày tải lên: 09/08/2014, 08:22

... Yang LP, Saah A, Hoover D: Maternal vitamin A deficiency and infant mortality in Malawi J Trop Pediatr 1998, 44(4):232-234 Semba RD, Miotti PG, Chiphangwi JD, Liomba G, Yang LP, Saah AJ, Dallabetta ... factorial trial assessing the impact of vitamin A, and of multivitamins excluding Vitamin A, on vertical transmission of HIV-1 and child mortality on pregnant women in Dar es Salaam, Tanzania (n = ... A retinyl palmitate + B-carotene, with additional vitamin A at delivery( n = 368) [17] Data on HIV infection at months were available for 502 children of the total 661 live births Another parallel...

Ngày tải lên: 10/08/2014, 05:20

... educationalists, patient advocates, and policy makers to guide program planning, service delivery, and quality improvement We and other researchers can use both the conceptual framework and taxonomy ... individual and organizational providers, and driving further research in this area, this knowledge may lead to greater patient-centred care delivery and improved healthcare outcomes Acknowledgements ... our review to arthritis and cancer Both represent prevalent conditions that have generated considerable research on patient involvement through education and self-management, but systematic reviews...

Ngày tải lên: 10/08/2014, 10:23

... and others have adopted the alternate strategy of gene therapy to prevent CABG failure [8] CABG is an "ideal" clinical scenario for gene therapy since saphenous vein can be genetically modified ... Multilabel Counter with recombinant luciferase (Promega) as a standard and normalised for total protein Ad-RGDLuc mediated a time-dependent increase in the level of transgene expression that was ... JS, Eagle KA, Gardner TJ, Garson A, Gregoratos G, Russell RO, SC Smith, McEntee CW, Elma MA, Pigmann GC, Starke RD, Taubert KA: ACC/AHA guidelines for coronary artery bypass graft surgery: A report...

Ngày tải lên: 14/08/2014, 19:22

... D10 D5 D N A R N A D N A D7 R N A D N A R N A D N A Controls Conjugate D10 R N A D N A R N A D N A D5 R N A D N A R N A D N A D7 R N A D N A R N A D N A D10 R N A D N A R N A D N A R N A Neg Pos ... -transfection R N A D N A R N A D7 D N A PCR Control Conjugate R N A D N A R N A D10 D N A D5 D N A R N A R N A D N A R N A D N A D7 R N A D N A R N A D N A R N A D10 D N A Neg R N A Pos 507 pb Figure ... Taq pol will be unable to amplified RNA whereas a PCR product would be observed if the RNA sample was contaminated with plasmid DNA No DNA plasmid contamination was observed for all the assayed...

Ngày tải lên: 14/08/2014, 19:22

... containing NotI) ATA AGA ATG CGG CCG CCC AGT TGT TCA ACT CAC CCT TCA and ([R] containing AgeI) TGT ACC GGT GGG TTG GAG AAA TCT CTG ACA GCT The MLC-2 construct was created by replacing the backbone ... described rat MLC-2 promoter[20] ([F] containing NotI) ATA AGA ATG CGG CCG CGA CCC AGA GCA CAG AGC ATC GT ([R] containing AgeI) TGT ACC GGT GAA TTC AAG GAG CCT GCT The cTnC construct was created by amplifying ... amplifying human genomic DNA: ([F] containing Not1) ATA AGA ATG CGG CCG CCA GCC TGA GAT CAC TGG GAC CAG A ([R] containing Age1) TGT ACC GGT CCA TGC TGG CGG CTC ACA GGA × 1010vg/mouse was administered...

Ngày tải lên: 14/08/2014, 19:22

... pIRES Human Malignant Melanoma DMEM Hygromycin M14 RacV12 Human Malignant Melanoma DMEM Hygromycin U87 Human Malignant Glioblastoma RPMI - LNCaP Human Malignant Prostate Cancer RPMI - Table Various ... specificity of substrate cleavage after an Asp residue (Degterev A et al, 2003) Caspase activation leads to the cleavage/degradation of a number of cellular proteins like PARP, Lamin A To make the picture ... cell shape and movement Actin filaments play a pivotal role in determining the shape and motility of a cell and also associate with the dynamic extensions like lamellipodia (Lagana A et al, 2000)...

Ngày tải lên: 16/09/2015, 08:31

... 40-50% of trabecular mass by 90 years of age [58] This results in a decline in the loadbearing capacity of the vertebral body For example, load bearing capacity in a lumbar vertebra can decrease from ... Diagram of the systems approach that may be applied to explain the multifactorial aetiology underlying osteoporotic vertebral fracture Bone, macroscopic, local and global factors may interact ... end plate The disc facilitates intersegmental movement while maintaining alignment of the vertebral column and has a primary role in attenuating and dispersing axial load With aging, the water...

Ngày tải lên: 03/11/2012, 09:49

... cross-domain authentication and session management The specifications also define provider metadata schemas that may be used for making a priori arrangements between providers The Liberty architecture ... entity-centric approach for IDM in the cloud The approach is based on: (1) active bundles—each including a payload of PII, privacy policies and a virtual machine that enforces the policies and uses a set ... negotiating a use of a cloud service It uses active bundle— which is a middleware agent that includes PII data, privacy policies, a virtual machine that enforces the policies, and has a set of...

Ngày tải lên: 31/07/2013, 09:44

... Med 44 Nakagawa K, Sasho T, Arai M, Kitahara S, Ogino S, Wada Y, et al Effects of autologous platelet-rich plasma on the metabolism of human articular chondrocytes Chiba and Ichihara, Japan Electronic ... A new technique for hemodilution, preparation of autologous platelet-rich plasma and intraoperative blood salvage in cardiac surgery Int J Artif Organs 1987;10:47–50 11 Antitua E, Andia I, Sanchez ... conditions in the United States 2nd ed Rosemont: American Academy of Orthopaedic Surgeons; 1999 Marx R, Garg A Dental and craniofacial applications of plateletrich plasma Carol Stream: Quintessence Publishing...

Ngày tải lên: 19/10/2013, 22:15

... projects: a meteorological field and satellite image display system, SAPO (http://sourceforge.net/projects/sapo), and the best available free numerical computer language to date, PDL (Perl Data Language) ... that does a real task on a real device I’ll use the simple and ubiquitous computer parallel port and the driver will be called parlelport The real “parlelport” driver: description of the parallel ... do: I once wrote two device drivers for two ISA Meilhaus boards, an analog to digital converter (ME26) and a relay control board (ME53) The software is available from the ADQ project Get the newer...

Ngày tải lên: 23/10/2013, 22:15

... International Journal of Advanced Manufacturing International Journal of Human Factors in Manufacturing Internationa Journal of Operations and Production Management International Journal of Production ... Summary of journals on agile manufacturing Agile manufacturin g research Agile manufacturing as a new strategy is captivating the attention of researchers A growing amount of research has arisen Using ... initiated by capacity analysis and workstation allocation (fractal cell creation), and after performing these tasks, an iterative algorithm that updates layouts depending on ¯ow assignments, and...

Ngày tải lên: 23/11/2013, 10:01

... projects: a meteorological field and satellite image display system, SAPO (http://sourceforge.net/projects/sapo), and the best available free numerical computer language to date, PDL (Perl Data Language) ... that does a real task on a real device I’ll use the simple and ubiquitous computer parallel port and the driver will be called parlelport The real “parlelport” driver: description of the parallel ... do: I once wrote two device drivers for two ISA Meilhaus boards, an analog to digital converter (ME26) and a relay control board (ME53) The software is available from the ADQ project Get the newer...

Ngày tải lên: 13/12/2013, 11:15

... that will affect the performance of KM have already been researched in great detail in the KM literature Organizational culture, in particular, has been advocated by various researchers as a ... a clear delineation and representation of those that are necessary An assortment of KM processes has been reported in the literature and, in fact, there are many standalone frameworks that have ... Knowledge Management 5(4): 300–310 103 RESEARCH ARTICLE Sena JA, Shani AB 1999 Intellectual capital and knowledge creation: towards an alternative framework In Knowledge Management Handbook, Liebowitz...

Ngày tải lên: 24/01/2014, 00:20

... salvaged blood into a 58-year-old woman operated for ovarian adenocarcinoma and 170 ml of salvaged blood into a 49-year-old female operated on for a large pelvic mass, which proved to be a gastrointestinal ... Potential risks accompanying the method are fat and air embolism and infection [4] Aminocaproic acid, desmopressin acetate, aprotinin, tranexamic acid, phytonadione and vasopressin are hemostatic ... Takemura M, Yamasaki M, Tanaka F, Shimizu H, Okamoto E, Hisamatu K, Ohama K, Tuji S, Hada Y, Nosaki T: Transcatheter arterial embolization in the management of gynecological neoplasms Gynecol Oncol...

Ngày tải lên: 13/02/2014, 06:20

... preventing and managing HIV infection through the use of family planning and dual protection, safe antenatal and delivery care, and STI prevention and treatment  Antenatal care, child health care and ... of cervical intraepithelial neoplasia (CIN) and advanced cervical disease, and at younger ages, than women in the general population, a risk which increases with a diagnosis of AIDS and low CD4 ... Amirkhanian YA, Kelly JA, et al HIV risk behaviour among gay and bisexual men in Budapest, Hungary International Journal of STD and AIDS 2002;13(3): 192–200 40 Carpenter LM, Kamali A, Ruberantwari...

Ngày tải lên: 13/02/2014, 15:20