adeno+associated+virus+mediated+gene+therapy+for+retinal+degenerative+diseases

... performed the virus inoculations, qPCR for cytokine detection and clinical evaluations on all mice evaluated in this study CP generated the single cell suspensions from lung tissue and performed ... interleukin; MyD88: myeloid differentiation primary response gene 88; PFU: plaque forming unit; PVM: pneumonia virus of mice; RSV: respiratory syncytial virus; SH: small hydrophobic (protein); TLR: toll-like ... value of all housekeeping genes was calculated to establish baseline expression, and ΔCt was determined by subtracting the mean C t for the housekeeping genes from the C t for each transcript of...

Ngày tải lên: 12/08/2014, 02:20

... purposes) Genetic Vaccines and Therapy 2008, 6:13 http://www.gvt-journal.com/content/6/1/13 Figure (see legend on next page) Page of (page number not for citation purposes) Genetic Vaccines and Therapy ... Florida Powell Gene Therapy Center (PGTC) and Irene Zolotukhin for providing technical expertise in producing the viruses used in this study We would also like to thank Stacy Porvasnik for assisting ... LC: Systemic gene therapy: biodistribution and longterm expression of a transgene in mice Proc Natl Acad Sci USA 1995, 92:9742-9746 Clark KR, Sferra TJ, Johnson PR: Recombinant adeno- associated...

Ngày tải lên: 14/08/2014, 19:22

... captured for quantification from 4–6 sections of hippocampus using a 4× objective for R1282 staining (Aβ) or a 10× objective for CD45 (microglia) and GFAP (astrocytes) Acquisition of images was performed ... Pharmaceuticals for providing reagents We gratefully acknowledge J Sears for expert technical assistance 18 References 10 11 12 13 14 15 16 Selkoe DJ: Alzheimer's disease: genes, proteins, and therapy ... placed in 10% buffered neutral buffered formalin for hours, processed, and embedded in paraffin The other hemi-brain was snap frozen and stored at -80°C for biochemical analysis of Aβ Anti-Aβ...

Ngày tải lên: 19/06/2014, 22:20

... thioflavin-S for 10 minutes Free-floating 4% paraformaldehyde-fixed, frozen tissue sections (30 μM) were stained for the presence of activated microglia with rat anti-mouse CD45 (1:3000; Serotec, Oxford, ... staining RWP performed the Aβ ELISA VMH performed the image quantification and immunohistology YL performed Aβ plasma ELISA and aided in the preparation of the manuscript PC performed Iba1 immunostaining ... [41] These data in IL-1 R1-/- mice demonstrate that IL-1 R1 is critical for most IL-1 -mediated signaling events tested We performed passive immunization with an anti-Aβ mAb in Tg2576 mice crossed...

Ngày tải lên: 19/06/2014, 22:20

... was 10,000 for each run We observed that the unadjusted p-values for DBP and ozone + DBP groups for male mice were 0.0147 and 0.0423, respectivly Therefore, even after correcting for multiplicity ... reaction mixture was overlaid with mineral oil, and incubated for at 94oC, followed by 30 cycles at 94oC for min, 55oC for min, and 72oC for min, with the last cycle containing a extension at 72oC ... sequencing As an internal control to check the methodology used for preparing mRNA to generate hprt cDNA, RT-PCR of the β-actin gene was performed on clones that did not yield hprt cDNA to ascertain...

Ngày tải lên: 07/08/2014, 18:20

... SP600125 (5 μM), and stimulated with rhIL-1β (10 ng/ml) for 30 minutes before assaying for p38, Erk and JNK phosphorylation, using cell-based ELISA For control of systematic variation, blank control ... and have been extensively used in several studies [29,30,66,67], the former is known for its varying potency for several isoforms of sPLA2 [28], while the latter is a broad-spectrum metalloproteinase ... Drug Administration, Center for Drug Evaluation and Research (CDER): Guidance for Industry Clinical Development Programs for Drugs, Devices, and Biological Products for the Treatment of Rheumatoid...

Ngày tải lên: 09/08/2014, 14:22

... time points and supernatants collected for virus sequencing Formation of syncytia was recorded on day 10 when virus replication was at the peak Results V38E virus fails to induce bystander apoptosis ... antibody clone KC57 (Beckman Coulter) for detection of virus infection and with activated caspase indicator, ZVAD-FITC (Promega) for apoptosis Flow cytometry was performed on the samples on a FACS ... acquired for each sample At day or postinfection, cells were also assayed for viability using the Cell Titer Glo (Promega) viability assay Supernatants from the cultures were assayed for virus replication...

Ngày tải lên: 11/08/2014, 21:21

... buffered formalin for two to three days and embedded in paraffin From the paraffin-embedded tissue blocks, μm-sections were cut and stained with hematoxylin and eosin for histological analysis For ... reached and remained stable for at least 30 minutes, the preparation was started and animals were allowed to recover from the surgical trauma for 15 minutes Thrombus formation was then induced ... catheter was placed into the right jugular vein, serving for application of fluorescent dyes For the study of microvascular thrombus formation, we used the cremaster muscle preparation as originally...

Ngày tải lên: 13/08/2014, 03:20

... al Evidence for human immunodeficiency virus type replication in vivo in CD14(+) monocytes and its potential role as a source of virus in patients on highly active antiretroviral therapy J Virol ... established a diagnosis of HAD using standard-of-care approaches for Thailand In general, the evaluation included participant and proxy informant reports of symptoms and function, the HIV macroneurological ... prior study conducted in Bangkok; therefore feasibility was established [17] We substituted the Brief Visual Memory Test-revised for the Picture Memory Test for logistical reasons as the latter...

Ngày tải lên: 31/10/2012, 15:34

Ngày tải lên: 25/01/2013, 09:29

Ngày tải lên: 21/06/2013, 01:26

... burning velocity (2.65-3.25 m/s for hydrogen and 0.3 m/s for diesel) compared to diesel In general the NO formed in hydrogendiesel dual fuel operation is higher for both port and manifold injection ... equation for the formation of smoke is C m H n + yO → y CO + n H + (m − y )C s (23) If m>2y, then C/O ratio exceeds unity resulting in the formation of smoke The fuel air ratio for smoke formation ... pumping loss ratio For hydrogen combustion the constant volume degree of combustion ( η gth ) is 99 % and for diesel it is 90 % whereas η u × (1 − φ w ) is 56 % for hydrogen and 61 % for diesel at...

Ngày tải lên: 05/09/2013, 15:28

... Give the past form of these verbs: be tell begin think was, were told began thought have get live help had got ... and the conclusion of the story Unit 1: A day in the life of Period 4: Writing Task 2.Task 2: Format of a narrative Climax Events Conclusion Unit 1: A day in the life of Period 4: Writing Task ... Writing Last year/ I / spend / summer holidays / a seaside Task town / The hotel / be modern / comfortable I have Task wonderful holiday / until / fire Task 3: Use the prompts below to build up...

Ngày tải lên: 18/09/2013, 05:10

Ngày tải lên: 19/09/2013, 14:10

... these languages, however, is that every request to the Web server for a Web page generates a new process on the server, leading to performance problems as visitor traffic increases Java offers a solution ... database to another, for example − without affecting other areas In the context of JSP, a multi−tier architecture involves the Web server for static HTML content, the application server for JavaBeans ... Thought For The Day Thought For The Day: Ever wonder if illiterate people get the full effect of alphabet soup? Alphabet Soup For The Sou 13 The...

Ngày tải lên: 19/10/2013, 02:15

Ngày tải lên: 28/10/2013, 17:15

... linh’ daily routine in minute -ask ss to remember activities and time’s activities of Linh -2 ms for teams go to the board and write answers -team which more correct answer is winner * checking...

Ngày tải lên: 02/12/2013, 19:11