Isolation and molecular characterization of genes associated with shoot regeneration of mustard (brassica juncea) in vitro

... ISOLATION AND MOLECULAR CHARACTERIZATION OF GENES ASSOCIATED WITH SHOOT REGENERATION OF MUSTARD (BRASSICA JUNCEA) IN VITRO GONG HAIBIAO (M.Sc SJTU) A THESIS SUBMITTED FOR THE DEGREE OF DOCTOR ... of genes associated with shoot regeneration from leaf disc explants of mustard (Brassica juncea) in vitro Discussion 84 The cloning of...

Ngày tải lên: 17/09/2015, 17:19

... A novel high-activity D-hydantoinase from Jannaschia sp CCS1 obtain optically pure amino acids, namely chemical and enzymatic syntheses Chemical synthesis gives racemic mixtures of amino acids ... precipitate fraction; sup, supernatant fraction The molecular weight standard (lane M) is indicated on the right A novel high-activity D-hydantoinase from Jannaschia sp...

Ngày tải lên: 18/02/2014, 08:20

... gAgTTggATgggTCCTCTgC CCAAACCAAAgCCAgTTTCATTCA CCAggTTTTgTATgAgTgCCgTA ACCTTggCCATCCTCTTCTT AgAAATCTTgACCCCCgTTC AAggAgCTCTTgACgTTggA TgCTAACAggTgggAAAACC CCTTCCAgCAgATgTggATT AgATTAggCAAggCgAggAT BEC87-GSP1 ... TgATTTTAgCTgCATgTgCACCTgAgAA CgTCATggAAATgTCTTAATTggCTTgCTg gAAgAAAACAAATTgggAggAggAgAA gCgTgTTCCAAAgAACACAATTCAgTgCCTT BEC-32BamHI DX24BamHI ggATCCTgATCTgTggATTgggTTTCgTgg ggATCCgggTg...

Ngày tải lên: 12/08/2014, 03:21

... products of the hazelnut LOX were compared with the reaction products of soybean LOX type I and authentic standards of 9- and 13-HODE As shown in Fig 4, 9-HODE is the main product of the hazelnut ... stages of seed development, and which is the only LOX detected in pea leaves [25] Considering the high level of expression of CaLOX2 in hazelnut seeds, it would be of...

Ngày tải lên: 21/02/2014, 00:20

... although, as V volvacea produces at least one other laccase isoform in addition to lac1 [1], this and possibly other laccase isoforms may be contributing to the total laccase activity detected at the ... primers The putative N-glycosylation site is boxed; *; stop codon The putative polyadenylation signals (TATAAA and CATAAA) are in white on a black background Ó FEBS 200...

Ngày tải lên: 07/03/2014, 15:20

... protein synthesis in the cell-free system of rabbit reticulocyte The protein synthesis decreased gradually with the increment of lamjapin in the reaction mixture The IC50 (the concentration of ... problem, the phenyl-Sepharose CL-4B column chromatography was chosen as the first step and it separated efficiently the target proteins from the inhibitory substan...

Ngày tải lên: 08/03/2014, 10:20

... was 0.7 and overall G-factor, a measure of the normality of the structure, was 0.0 Fig SDS/PAGE and activity analysis of recombinant cellobiohydrolase (A) SDS/ PAGE [10% (w/v) gel] analysis of ... (1985) Isolation and characterization of the endoglucanases of Talaromyces emersonii Biochem J 225, 365–374 29 McHale, A & Coughlan, M (1988) Purification of beta...

Ngày tải lên: 23/03/2014, 13:20

... for the Ndh membrane subcomplex The Ndh antibodies recognized the 46, 28, 18 and 12 kDa polypeptides, corresponding to the NdhH, -K, -J and -E subunits of the Ndh complex The intact Ndh complex, ... et al of the ndh genes is much higher in BS chloroplasts, and elevated amounts of the Ndh complex have been found in these plastids [18] The functio...

Ngày tải lên: 23/03/2014, 13:20

... alignment of the N-terminal sequence of ChPLA2 with those of TPLA2 [14], OPLA2 [15], DrPLA2 [13], PPLA2 [20], bovine PLA2 [20], and human PLA2 [21] The N-terminal sequences of bird PLA2s exhibit ... TPLA2, OPLA2 DrPLA2, bovine PLA2 and human PLA2 Identical amino acids are in bold, and homologous amino acids are in italic ChPLA2 PPLA2 TPLA2 OPLA2 DrPLA2 Bovine PLA2 Human PLA2 :AL...

Ngày tải lên: 30/03/2014, 01:20

... were eluted in two phases with 300 mL of linear gradient of 0–0.6 M NaCl and 50 mL of linear gradient of 0.6–1 M NaCl at a flow rate of 0.8 mLÆmin)1 Fractions of mL were collected and those displaying ... results of studies leading to the conclusion that at least two distinct soluble enzymes of P denitrificans exhibit an activity of Fe(III) reductase These enzymes...

Ngày tải lên: 30/03/2014, 13:20

... for retrieval of viral pathogens from infected leaf tissues and for the detection of viralderived transgene sequences in transgenic plants Results Use of FTA for sampling, retrieval and PCR-based ... Effective methods for sampling, storage and retrieval of viral pathogens from infected plant tissues allows not only identification of the viral pat...

Ngày tải lên: 19/06/2014, 08:20

... for retrieval of viral pathogens from infected leaf tissues and for the detection of viralderived transgene sequences in transgenic plants Results Use of FTA for sampling, retrieval and PCR-based ... Effective methods for sampling, storage and retrieval of viral pathogens from infected plant tissues allows not only identification of the viral pat...

Ngày tải lên: 20/06/2014, 04:20

... of infectious bronchitis virus isolated in the UK Vet Rec 2008, 162, 99-100 11 Gough RE, Randall CJ, Dagless M, Alexander DJ, Cox WJ, Pearson D A ‘new’ strain of infectious bronchitis virus infecting ... region of the S1 gene of infectious bronchitis virus Arch Vrol 2000, 145, 291-300 Yu L, Wang Z, Jiang Y, Low S, Kwang J Molecular epidemiology of infectious...

Ngày tải lên: 07/08/2014, 23:22

... proteins CEBFs (CE1 binding factors) DNA -binding and transcriptional activities of CEBFs Binding of a number of CEBFs, which were isolated as multiple isolates (Table 1), to the CE1 element was ... Lee et al.: Isolation and functional characterization of CE1 binding proteins BMC Plant Biology 2010 10:277 Submit your next manuscript to BioMed Central and take f...

Ngày tải lên: 11/08/2014, 11:21

... resolutive HBV infection, HBV/HCV co-infected with overt HBV infection (HBsAg positives) and < /b> HBV/HCV co-infected with occult HBV infection (HBsAg negatives) The 30 patients with uncertain HBV status ... E, Coppola N, Scolastico C, Filippini P, Santantonio T, Stroffolini T, Piccinino F: Virologic and < /b> clinical expressions of < /b> reciprocal inhibitory effect of <...

Ngày tải lên: 12/08/2014, 01:21