biochemical and molecular analysis of bone cells

... IL-1b-treated cells (C,D) n-3 PUFAs inhibit cyclin D1 expression and phosphorylation of Rb Lysates of whole cells were prepared from cells treated as described above and 20 lg aliquots of protein ... control of SMC proliferation 4466 S Bousserouel et al (Eur J Biochem 271) Modulation of cyclin D1 synthesis and hyperphosphorylation of Rb by n-3 and n-6 PUFAs Induction of cyclin D1 is one of the ... for 16 h The cells were then fixed and the BrdU incorporated was quantified by using a commercial detection kit (Roche Molecular Biochemicals) Ó FEBS 2004 Anti-mitogenic action of EPA and DHA (Eur...

Ngày tải lên: 19/02/2014, 16:20

... The HEK293 cells were transfected with plasmids encoding Rz-1, Rz-2 and with their inactive versions (Rz-1i and Rz-2i) Transfections of the cells with lipofectin (NO) only or lipofectin and an empty ... to the amount of BACE in the control transfected cells A protein mixture of known molecular mass was used as a marker to establish the band of BACE protein with a molecular mass of  70 kDa As ... several months without any decomposition of the RNA Determination of the level of BACE mRNA and ribozyme RNA expression in mammalian cells The level of BACE mRNA and ribozyme RNA was monitored by RT/PCR...

Ngày tải lên: 08/03/2014, 08:20

... indicated times Cells in the absence of any of these were taken as the control (Con) Two micrograms of total RNA were reverse transcribed with random primers and the levels of PEPCK and G6Pase mRNA ... Each band was analysed densitometrically and the values are depicted after normalization of PEPCK and G6Pase bands with those of 18S rRNA (B) Each point is the mean ± SEM of three sets of experiments ... primary cells and animal models but, as a preliminary step, they unravel one of the mechanisms of TNFamediated withdrawal of insulin action in HepG2 cells Results Incubation of HepG2 cells with...

Ngày tải lên: 16/03/2014, 02:20

... mM potassium glutamate and used subsequently for biochemical analysis The amount of FAD cofactor of the NifL fractions was calculated using a UV/Visual spectrum at 450 nm and the extinction coefficient ... 280 and 320 nm (MD) Preparation of inside-out vesicles of K pneumoniae One litre cultures of K pneumoniae cells were grown under nitrogen- and O2-limited conditions, harvested at a D600 value of ... absence of O2, when the flavin cofactor is reduced [16–19] Thus, under anaerobic conditions in the absence of ammonium, reduction of the flavin moiety of NifL is required to relieve NifL inhibition of...

Ngày tải lên: 17/03/2014, 10:20

... expression analysis with histomorphometry allows the exploration of any relationships between gene expression and indices of bone architecture and bone remodelling Interestingly, both OCN and ALP ... 0.008 and r = 0.70, p < 0.008, respectively; Fig- Comparison of bone structural and turnover indices between OA and control individuals Bone histomorphometry was performed on IT trabecular bone ... knowledge about the molecular and cellular mechanisms involved in the increase or maintenance of bone mass in OA This study of the IT region of the proximal femur in primary hip OA and non-OA postmortem...

Ngày tải lên: 09/08/2014, 08:23

... role in the stimulation of B lymphocytes for antibody production TNF-a together with IL-6 may boost proliferation and differentiation of B cells, and proliferation of T cells As a result, all ... Kinetics of replication of different subtypes of influenza A virus in NCI-H292 cells NCI-H292 cells were infected with different influenza virus subtypes: H1 - H1N1/2002, H5 - H5N1/ 2004, and H9 ... of H9N2/1997 only showed a transient elevation at hours post-infection In contrast, the elevation of TGF-b2 level of H1N1/2002 was sustained and increased with time reaching 2-3 folds at 18 and...

Ngày tải lên: 12/08/2014, 02:20

... also found Because TLR is the product of TLRi and RA, this can be the result of the negative correlation of RA and RS and the lack of correlation between TLRi and RS However, no correlation Genome ... blue) between RA and PA, PA and TLR or TLRi, RA and TR and between TR and TLR or TLRi Some of these correlations have been described previously [8,11,17,19] The correlation between TR and TLR was ... sizes were equal to that of genes in each considered group and subsequent computation of average and standard deviations for each variable An estimation of the average standard deviations (aSE)...

Ngày tải lên: 14/08/2014, 08:20

... probe RESULTS AND DISCUSSION A total of 69 well-banded chromosome spreads were scored Among 579 grains counted on the chromosomes (Fig 1), 500 (86%) were observed on chromosome and out of the 500 ... locate the silver grains and identify the chromosomes The chromosomes were classified according to the G-band pattern, defined by the Committee for the standardized karyotype of the Domestic Pig (1988) ... the presence of a 1,000-fold excess of sonicated salmon sperm DNA The precipitate was diluted in the following solution: 50% ionized formamide; 10% dextran sulfate; and 1/5 volume of SCCP (0.2...

Ngày tải lên: 14/08/2014, 19:23

... other (in case of lymphoma h1) and SMG-1, the gene of ribosomal protein S8 (in case of lymphoma h2), as well as several genes of unknown function (9 and in the case of lymphoma h1 and h2, respectively) ... analysis of the transcription levels of the set, ND4, and SMG-1 genes in human spontaneous lymphomas and normal B- and Tlymphocytes (a) 32P-labeled PCR-fragments of the set and a-myb oncogenes, and ... cDNA population of lymphoma h1 as tracer, and the cDNA population of lymphoma h2 as driver; and vice versa, 2) the cDNA population of lymphoma h2 as tracer, and the cDNA population of lymphoma h1...

Ngày tải lên: 02/11/2012, 11:08

... understanding of the influence of the molecular function is necessary, as well as of biochemical processes and of the biophysical properties provoked amongst others by a moderate repression of the ... the behaviour of the cells, which may alter the biochemical effect of the transported cargo and which could consequently influence the interpretation of the results Table List of the examined ... block and the software version 1.2 The 10µl reaction consisted of 25ng of transcribed RNA (1µl), 1pmol Universal Probe (0.1µl), 50pmol each of forward + reverse primer (0.5µl), water (3.4µl) and...

Ngày tải lên: 03/11/2012, 11:44

... representative TEM images of mitochondria in eWAT (Figures 2A and 2B) and iBAT (Figures 2C and 2D), respectively, and the analyses of mitochondria number (Figure 2E) and area (Figure 2F) In the ... The number and area of mitochondria in the eWAT and iBAT A-B Representative TEM images of eWAT C-D Representative TEM images of iBAT (Arrows point to mitochondria) E: The analysis of mitochondrial ... response to PM2.5 exposure, in terms of the expression of BAT-specific and WAT-specific gene profiles by real-time PCR analysis UCP1 uncouples substrate oxidation and electron transport through the...

Ngày tải lên: 06/03/2014, 19:20

... date, four isoforms of PDK (PDK1–4) and two isoforms of PDP (PDP1–2) have been identified in humans [3–5] These isoforms display unique tissue distribution [3–5] and varied kinetic and regulatory ... Regulation of hPDK2 and hPDK4 gene expression Table The effects of PPAR a, d and c agonists on PDK2 and PDK4 transcript abundance expressed as a percentage of basal levels Results are the means ± SEM of ... expression of the human PDK2 and PDK4 isoforms In addition to our findings, insulin has been shown to decrease the mRNA for PDK2 and PDK4 in 7800C1 hepatoma cells, human rhabdomyosarcoma cells and whole...

Ngày tải lên: 07/03/2014, 17:20

... two types of probes For analysis of differential gene expression, 1473 and 1703 beads were collected in fractions U1 and U2 of the up-regulated genes, and 3550 beads in fraction D of the down-regulated ... some of them might be missorted at the gates, which would expand the list of genes Of the beads collected at gates U1 and D on fluid microarray analysis, 89 and 87% were regarded as up-regulated and ... incubated in the presence (lanes and 4) or absence of TM (lanes and 2) for 48 h Proteins were extracted, digested with (lanes and 4) or without (lanes and 3) Endo H, and then subjected to SDS ⁄ PAGE...

Ngày tải lên: 07/03/2014, 21:20

... Protein and total RNA were extracted from various EOC cell lines, HEK293 and LNCaP cell lines (A, B) Expression levels of protein and mRNA, and levels of p53, ANX4 and the known p53 targets, p21 and ... Protein and RNA were extracted from two CCA (OVTOKO and OVISE) cells lines and one non-CCA (MCAS) cell line, and then ANX4 protein (A) and mRNA (B) levels were compared by western blotting and real-time ... Infrequent p53 gene mutations and lack of p53 protein expression in clear cell sarcoma of the kidney: immunohistochemical study and mutation analysis of p53 in renal tumors of unfavorable prognosis...

Ngày tải lên: 22/03/2014, 16:20

... perivenous cells, code for 6-phosphofructo-2-kinase, which produces fructose-2,6-bisphosphate, an allosteric activator of the glycolytic enzyme 6-phosphofructo-1kinase and an inhibitor of the gluconeogenic ... differences in mRNA levels of four enzymes of the urea cycle Whereas the zonal-specific expression of the main enzymes of drug and xenobiotic metabolism has been the subject of extensive research (e.g ... nanograms of total RNA were reverse transcribed into cDNA by avian myeloblastosis virus-RT (Promega, Mannheim, Germany) using standard methods and oligo(dT)18 and random(dN)6 primers Expression analysis...

Ngày tải lên: 23/03/2014, 10:20

... B1 isoform) of other insect EcRs and a strong degree of identity was confined to the two ends of N-terminal region of the A/B domain Using a B1 isoform-specific probe from the A/B region of PIEcR, ... Intermediate levels of cell proliferation were attained at · 10)8, 10)8 Fig Effect of 20E and JH II on the proliferation of IAL-PID2 cells The IAL-PID2 cells were seeded at 1.5 · 106 per flask and cultured ... efficiently the effects of 20E both on cell proliferation and morphological changes of IAL-PID2 cells Isolation and characterization of P interpunctella EcR-B1 mRNA Cloning of a PIEcR cDNA frament...

Ngày tải lên: 23/03/2014, 13:20

... ecdysteroids and ecdysone mimics can induce and ⁄ or suppress endogenous genes in RKO and other mammalian cells and promote apoptosis A more comprehensive understanding of possible pleiotropic effects of ... lymphoid cells, myeloid cells such as thymocytes, B cells, monocytes, and nonlymphoid cells such as fibroblasts, kidney cells and neurons [40,41] Huang et al [42,43] showed that the expression of the ... total of 20 lL reaction volume containing lL of cDNA, lL of each of forward and reverse sequence specific primers (from 10 lm primer stock), 10 lL of supermix (Bio-Rad Laboratories) and lL of nuclease...

Ngày tải lên: 30/03/2014, 03:20

... statistical analysis and drafted the manuscript KE, TB and KL carried out and supervised the ISH SWS and KE scored the cores KAS, SAS, SA and HS contributed in the clinical and demographic database and ... cores there was a mixture of stromal cells and tumor cells By morphological criteria only tumor cells were scored staining intensity Page of All samples were anonymized and independently scored ... the cytoplasm of normal epithelial cells in lung tissue Based on morphological criteria, inflammatory cells (macrophages, lymphocytes, granulocytes and plasma cells) , pneumocytes and fibroblasts,...

Ngày tải lên: 18/06/2014, 16:20

... of the femoral condyles and tibial plateaus of both the operated and non-operated control stifles was analyzed for histologic, biochemical, and molecular measures of cell and matrix changes This ... identifying and developing diagnostic methods and markers, as well as strategies for prevention and treatment of OA in the earliest stages of disease harvesting, analysis and interpretation of data, ... observed in the HP content of the tissues between ACL-X and control joints for any of the regions tested Error bars indicate standard error of the mean Values are μg of HP/mg of tissue wet weight Differential...

Ngày tải lên: 20/06/2014, 00:20