testing and spectrometric characterization of polymers

... composed of  35% a helix and a small amount of b sheet, whereas PrP Sc appears to have elevated levels of b sheet [7,8]. Higher resolution studies of the structure of PrP C have made use of NMR and ... thor- ough understanding of the folding and interactions of PrP in its tethered conformation on the plasma mem- brane. The interaction of PrP with different lipid compo- nents is complex and is not ... followed by dialysis and subsequent removal of precipitated protein was typically 80% of the reduced protein obtained. This gave an overall yield of PrP-React of 12–20 mg per litre of culture. M. R....

Ngày tải lên: 23/03/2014, 10:21

... FEBS 3579 characterization and evaluation of HYD Js from Jannaschia sp. CCS1. Characterization of HYD Js To explore the possible cause of the higher specific activity for conversion of d,l-p-HPH ... positions of the Ca of Ala156 in HYD Js and the Ca of Met156 in HYD Bp located in the SGL3, which leads to the increased size of the substrate bind- ing pocket of HYD Js . The side chain of Ala156 ... [27]. The three SGLs of HYD Js , i.e. SGL1, SGL2 and SGL3, correspond to residues 60–71, 91–99 and 151–161, respectively. On the basis of the homol- ogy model, the SGLs of HYD Js and HYD Bp (PDB accession...

Ngày tải lên: 18/02/2014, 08:20

... of Glu, glycosylation of Ser or Thr, bromination of Trp, sulfation of Tyr, cyclization of N-terminal Gln, and epimerization of several different residues It is quite rare for such a variety of ... Du3,* and Chunguang Wang1 Institute of Protein Research, Tongji University, Shanghai, China Institute of Biochemistry and Cell Biology, Shanghai Institute of Biology Sciences, Chinese Academy of ... structure of conomarphin was similar to those of other conotoxin cDNAs The cDNA-encoded precursor of conomarphin consisted of a conserved M-superfamily signal peptide of 25 residues, a proregion of...

Ngày tải lên: 18/02/2014, 17:20

... increase of 0.2 nm, and a bandwidth of 1 nm Thermal stability of Rep245 was measured by incubating the protein at 60, 70 and 80 °C for 30 min and then recording the CD spectra of ... plausibly involved in DNA recognition and binding and is positioned left of the active site [14]. Rep245 is both capable of de novo synthesis of DNA primers and of elongating them. Long DNA extension ... Ca atoms of the catalytic triad, are positional homologs of the R148 and K300 residues of P. furiosus archaeal primase, both of which are known to play a pivotal role in the activity of this protein...

Ngày tải lên: 18/02/2014, 18:20

... electrophoresis of the PCR product indicated the presence of two distinct bands between 1000 and 1100 bp of roughly the same extent (not shown) Cloning and nucleotide sequencing of these two products ... the mean of three independent experiments and are presented as copies of the target variant per 109 copies of 18S RNA Quantitation of ACMSD variants in liver, kidney and brain An isoform-specific ... the software swiss-pdbviewer and visual molecular dynamics [49] Acknowledgements We thank G Magni and S Ruggieri for helpful comments and suggestions and T Begley (Department of Chemistry and...

Ngày tải lên: 19/02/2014, 02:20

... is formed by an insertion of  230 residues (residues 133–367) between strands D and E and a smaller insertion of  50 residues (residues 404–458) between strands F and G. The hypervariable loop, Table ... ionic strength of the solvent environment and ⁄ or presence of small organic compounds (dioxane and MPD) seems to favor formation of dodecahedra over isolated pentamers. Previous structural analysis of the ... domain formed by two b-sheets and a distal domain formed by insertions between the strands of the jellyroll domain (Fig. 3). Antiparallel b-sheets made up of strands CHEF and BIDG pack against each...

Ngày tải lên: 19/02/2014, 06:20

... capacity of fish xenobiotic metabolism [28] and raises the possibility of arachi- donic acid based signalling. Zebrafish express both cyclooxygenase (cox)-1 and -2 and the primary prostaglandin end ... pmol of template and 0.5 U of Pfu polymerase The temperature cycles 1698 A Bresell et al were 30 s at 94 °C, 1 min at 40 °C and 2 min at 72 °C,... isolated from a number of clones and ... above and in [1]). We investigated representative gene products from the E. coli and Synechocystis sp. bacteria further, to gain insight into the function of these proteins and the evolution of...

Ngày tải lên: 19/02/2014, 17:20

... products of the hazelnut LOX were compared with the reaction products of soybean LOX type I and authentic standards of 9- and 13-HODE. As shown in Fig. 4, 9-HODE is the main product of the hazelnut ... part of the world production adsorbed by the pastry industry. Hazelnut seeds contain reasonable levels of linoleic and linolenic acid (about 10 and 0.2%, respectively, of the total fatty acids) and ... lm particle size) with a solvent system of hexane/propan-2-ol/acetic acid (100 : 5 : 0.1, v/v/v) and a flow rate of 1 mLÆmin )1 . Authentic standards of 9- and 13-HODE were purchased from ICN. HPLC...

Ngày tải lên: 21/02/2014, 00:20

... one of the most abundant enzymes in the biosphere [1,2]. It consists of the Fe-protein (dinitrogenase reductase, also referred to as NifH), an a 2 dimer of the nifH gene product and of the MoFe-protein ... Mass spectrometric characterization of the covalent modification of the nitrogenase Fe-protein in Azoarcus sp. BH72 Janina Oetjen 1 , Sascha Rexroth 2 and Barbara Reinhold-Hurek 1 1 ... integrin, histones, and even DNA, as a regulatory process [27]. Detection of ADP-ribosylation on proteins is often accomplished by radioactive labeling of the donor mol- ecule NAD + and autoradiography....

Ngày tải lên: 07/03/2014, 02:20

... sheets, subjected to TLC and quantification of radiolabelled ADP and AMP The kinetics of [3H]AMP and [3H]ADP formation in the absence of tRNAGlu (black symbols) or of both L-Glu and tRNAGlu (grey symbols) ... GluRS and GluTR, whose spectrum and DLS signal are shown in Fig S11 The migration positions of GluTR and GluRS, were determined by comparison with those of a homogeneous sample of GluRS (S) and of ... sequence and the mass of the fragments, and from the kinetics of the process (Figs S7–S9 and Table S5) we concluded that the main sites of proteolytic cleavage are at the C-terminus of the predicted...

Ngày tải lên: 07/03/2014, 03:20

... syringaldazine and 2,6-DMP were pH 5.6 and 3.4 mg protein)1 and pH 4.6 and 2.8 mg protein)1, respectively In standard assay mixtures, the velocity of ABTS oxidation was maximal at 45 °C The dependence of ... we have purified and characterized one of the laccase isoforms, cloned and sequenced the cDNA encoding the enzyme protein, and examined the effect of copper and various Correspondence to J.A Buswell, ... doi:10.1046/j.1432-1033.2003.03930.x Biochemical and molecular characterization of a laccase from the edible straw mushroom, Volvariella volvacea Shicheng Chen, Wei Ge and John A Buswell Department of Biology and the Centre for...

Ngày tải lên: 07/03/2014, 15:20

... Molecular and functional characterization of novel CRFR1 isoforms from the skin Alexander Pisarchik and Andrzej Slominski Department of Pathology and Laboratory Medicine, University of Tennessee ... isoform where exon 3 and exon 6 are spliced out (U16273); CRFR1d isoform where exons 6 and 13 are missing (AF180301); CRH-R1e with deletion of exons 3 and 4 (AF369651); CRFR1f with deletion of ... with deletion of exon 11, 27 basepairs of exon 10 and 28 basepairs of exon 12 (AF369653); and CRFR1h with addition of a cryptic exon (AF374231) [15–18]. In rodents, three CRFR1 isoforms have already...

Ngày tải lên: 07/03/2014, 15:20

... 2B that VBARP-L and VBARP-S are coded by these specific exons VBARP-L and VBARP-S code for precursor proteins of 627 and 435 amino acids and with calculated peptide masses of 69 and 49 kDa, respectively ... Expression and biochemical characterization of VBARP The predicted sizes of the proteins encoded by VBARP-L and VBARP-S are 69 and 49 kDa, respectively To test this, C-terminal V5-tagged VBARP-L and ... distribution of VBARP is independent of the cycling stage of the cells Identification of biological function(s) of VBARP using a siRNA assay To examine the role of endogenous VBARP in the regulation of...

Ngày tải lên: 07/03/2014, 21:20

... Susan Manzi 4 and M. Ilyas Kamboh 1 1 Department of Human Genetics, Graduate School of Public Health, University of Pittsburgh, Pittsburgh, PA, USA 2 Department of Environmental and Occupational ... of Public Health, University of Pittsburgh, Pittsburgh, PA, USA 3 Northwestern University, Feinberg School of Medicine, Division of Rheumatology, Chicago, IL, USA 4 Division of Rheumatology and ... HepG2 cells, the effects of serial deletion analysis of the APOH 5¢ flanking region in COS-1 and HepG2 cells and cross-species conservation of the APOH promoter sequence. The variant alleles of three single...

Ngày tải lên: 15/03/2014, 10:20

... to Erlandsen and Stevens [22] The details of the interactions displayed by residues in the neighborhood of Q301 were analyzed in the structure of the ternary complex of human PAH with BH4 and thienylalanine, ... family and a shorter fragment of 253 bp present only in the proband, as well as in his mother who bears the same mutation (Fig 3) Direct sequencing of both cDNA bands confirmed the skipping of the ... varies in distinct populations and geographic areas [7–9] and a number of them have been analyzed and characterized in vitro [10,11] Identification of the mutations and subsequent in vitro expression...

Ngày tải lên: 16/03/2014, 01:20

... sequence. b The amino acid composition analyzed by amino acid analysis. c Sum of the numbers of Asp and Asn. d Sum of the numbers of Glu and Gln. Barnacle surface-cement protein Y. Urushida et al. 4338 FEBS ... polycrystalline gold and alkylated gold. The arrows and thick arrows indicate the starts of sample loading (2 l M) and washing by the running buffer, respectively. The processes of sample loading and washing ... cytosolic space of several organ- isms, including bacteria and fish [17], the Ala and ⁄ or methyl group of Thr on the molecular surface of the protein are known to be essential in the process of binding...

Ngày tải lên: 16/03/2014, 05:20

... protein composed mainly of a helices (Figs 1 and 2). The core of Iba2 is a pair of EF-hand motifs, denoted as EF-hands 1 and 2, each consisting of two a helices (aA, aB and aC, aD, respectively) ... cross-linking efficiency of Iba1 and Iba2 or in the overall morphology of the generated filament bundles. Calcium affinity of Iba1 and Iba2 Homodimerization and actin binding of Iba1 and Iba2 were similar ... may be concluded that EF-hand 1 of the Iba proteins is not capable of functional Ca 2+ binding. Function of EF-hand 2 In both Iba1 crystal structures, the loops of EF-hand 2 adopt essentially the...

Ngày tải lên: 16/03/2014, 06:20

... used as the standards and their elution volumes, Ve, were determined The standard curve was plotted with the logarithm of molecular mass against Ve/V0 of the standard protein Peptidase and ATPase ... operation, and disassembly of DNA–protein complexes [13] Clp/ HSP100 proteins act as molecular chaperones and play a role in the unfolding of substrates and their translocation into the cavity of the ... interaction [36] Analysis of promoter and heat-induced transcription of Bt-lon The lon gene of E coli and B subtilis belongs to the heatshock regulon, the transcription of which is increased on...

Ngày tải lên: 16/03/2014, 16:20

... in the case of Pf LDH and red in the case of Pf MDH Comparative expression of Pf MDH, Pf LDH and Pf MQO and effect of gossypol The effect of gossypol on the expression of Pf LDH and Pf MDH in ... optimum pH for oxidation of malate in the presence of NAD was 10.2 and for the reduction of OAA using NADH as the cofactor it was 7.0 Further kinetic characterization of enzyme was performed at ... specificity loop of Pf MDH shares a sequence identity of 65% with that of the MDH from C tepidum and E coli As expected, the structure of this loop in Pf MDH closely resembles that of ligand bound activated...

Ngày tải lên: 16/03/2014, 18:20

... reaction. Here we describe the further biochemical and spect- roscopic characterization of BphP. Results Expression, purification and initial characterization of recombinant P. aeruginosa phytochrome The ... NaOH apo Pfr Pr protein 32 P Fig. 6. Autoradiogram of BphP. Autoradiogram after [ 32 P]ATP[cP] labeling, SDS ⁄ PAGE and electroblotting. (A) Autoradiogram of the apo and holo forms of BphP. (B) Stability of the autophosphoryla- tion ... signature (Fig. 1B) with maxima of 700 and 754 nm for the Pr and Pfr form, respectively. These far-red absorbance maxima seem to be typical of biliverdin-binding phytochromes and represent the most red-shifted...

Ngày tải lên: 16/03/2014, 18:20