Isolation and characterization of the novel human gene, MOST 1

... impact on MOST- 1 function 11 9 MOST- 1 Protein 12 1 Aggregation and implication of MOST- 1 function 12 3 Interactors and their possible function with MOST- 1 126 MOST- 1 Expression and Cell Cycle 13 2 Current ... synchronization studies 94 v 4 .11 Yeast two hybrid screening 10 2 4 .12 Overexpression and RNA interference studies 11 0 CHAPTER 5: DISCUSSION Strate...

Ngày tải lên: 16/09/2015, 17:13

... combinations of degenerate and specific primers and by rescreening the cDNA libraries The partial clones of XHIVEP2 and -3 covered 5.9 and 3.2 kb of the respective 3¢ ends and included 3¢-UTRs and poly(A)-tails ... analyze the function of XHIVEP in the context of Xenopus embryogenesis (data not shown) However, the interpretation of the in vivo role of...

Ngày tải lên: 30/03/2014, 13:20

... very close to that of the mouse Muc4 (Table 1) The size of intron of the human MUC4 has been estimated to be  15 kb by restriction mapping [12] and to be at least 20 kb by our analysis of the human ... small mucin [26] The consensus sequence of the Muc4 tandem repeat is very close to that of SMC Nevertheless, the tandem repeat domain of Muc4...

Ngày tải lên: 08/03/2014, 23:20

... the mechanism of transcriptional regulation of the FSH subunit gene promoter of the Chinook salmon The Chinook salmon FSH subunit gene and 5’ flanking sequence will be isolated, sequenced and ... regard to the size of introns and 3’ untranslated region (3’ UTR; Figure 3) Compared to the 0.3 – 0.35 kb size of intron of the LHβ genes, the size...

Ngày tải lên: 08/11/2015, 17:15

... aminoacids at the N-terminus of PHI (sequence: SKVYLALQDND) were compared with the sequences of the so called ‘intermediate components’ from two other PHs The N-terminal sequence of PHI from A radioresistens ... one PHR and one PHO (abc) protomer The hypothesis of a direct PHI phenol interaction is quite unlikely, because of the fact that the addition of ph...

Ngày tải lên: 21/02/2014, 00:20

... human and a murine NICN1 cDNA clone from the IMAGE collection and determined the complete sequences of these cDNAs The comparative analysis of the human, canine, and murine NICN1 cDNA revealed a ... CACCAGgtcagctgggcctca 423 418 bp (exon 3, 11 4 bp) … CCAAAGgcaagtgactttgca 4 01 bp (exon 4, 72 bp) 495 … CTTGAGgtaagctctctaaca 3 71 bp (exon 5, 10 5 bp) 600 … TTCGACgtgag...

Ngày tải lên: 23/03/2014, 21:20

... Figure analysis of methyl halides, methanethiol, and DMS from R sativus GC-MS GC-MS analysis of methyl halides, methanethiol, and DMS from R sativus (a) GC-MS spectrum of methyl halide standards ... the emission profiles of methyl halides from some plants and the characterization of the enzymatic properties of recombinant R sativus HTMT (RsHTMT) Resul...

Ngày tải lên: 12/08/2014, 03:21

... strain to those of the other simian rotavirus strains, SA11 and RRV Materials and methods Rotavirus isolation The YK-1 strain of simian rotavirus was isolated from the diarrheal stool of a 2-year-old ... was based on a Jennerianapproach prompted by studies indicating that animal and human rotaviruses share a common group antigen and that experimental animals immunize...

Ngày tải lên: 20/06/2014, 01:20

... 11 9 11 9 11 8 11 6 11 7 11 9 92 12 6 15 1 14 2 11 8 11 8 11 3 11 2 14 2 14 0 16 6 PFYQGHKN Figure The two coffee candidates for NDR1 protein belong to the NHL family Putative Arabidopsis orthologs of CaNDR1a/b ... Biology 2 011 , 11 :14 4 http://www.biomedcentral.com /14 71- 2229 /11 /14 4 (a) CE (b) 12 0 Page 10 of 17 μ PM PMA2 86 NDR1 47 34 (c) (μg) 10 15 PM...

Ngày tải lên: 11/08/2014, 11:21

... degree of affinity and specificity for Tom70, and with a KD of  nM compares very well with affinities reported for camelid VHH domains (that vary in the range 2–300 nM [36]) and for scFv and disulphide ... generate The human Tom70 receptor is an important example of such refractory targets, and the generation of a high-affinity reagent specific for the...

Ngày tải lên: 17/03/2014, 10:20

... describes the relationship between the heme core description and the spectroscopic and redox properties of each identified heme from the NrfHA complex Conclusions D desulfuricans ATCC 27774 ccNiR ... this communication, we report for the first time the isolation and biochemical characterization of D desulfuricans ATCC 27774 ccNiR subunits The st...

Ngày tải lên: 21/02/2014, 00:20

... of parallel b strands associated to an antiparallel strand (b2) and is surrounded by helices (a1 , a2 , a3 , a7 and a8 ) The second domain consists of helices a4 , a5 and a6 all clustered on the top ... indicating that Rv1399c is composed of 22% of a- helices, 25% of b-strand and 39% of random coil The catalytic triad is located at the bottom of a s...

Ngày tải lên: 07/03/2014, 16:20

... box) Recognition of proteins containing CP2- binding motifs and verification of the CP2- binding ability of REST and YY1 that contain the HXPR motif As stated above, none of the 12-mer peptide sequences ... 306–396) of the SPXX region on CP2 is involved in the binding of the ASR motif as well as HXPR (Fig 3) However, we not know whether these two motifs...

Ngày tải lên: 16/03/2014, 18:20

... (Fig 3A) These results indicated that the b-aryl ether cleavage enzyme accumulated and was stable in the extracellular fraction The extracellular fraction of 2BW-1 generated abundant GG and 4MU ... radiolabeled water was not observed with guaiacol It was clear that the b-aryl ether cleavage enzyme catalyzed the addition of two molecules of H2O (at C...

Ngày tải lên: 17/03/2014, 03:20

... results, the expression vector pLac1-B was selected to characterize the recombinant laccase from A niger Immunodetection of the recombinant laccase and expression of the corresponding gene in A niger ... wild-type laccase from P cinnabarinus Immunodetection of the laccase was performed using antibodies raised against the P cinnabarinus laccase...

Ngày tải lên: 17/03/2014, 11:20