isolation and characterization of resident mesenchymal stem cells in human glomeruli

... shown in pellet formation and toluidine blue staining In this study, we used cUCB-MSCs at 3∼5 passage Generally, increasing the passage number of adult stem cells often leads to a decline in the ... in isolating enough multipotent stem cells from human umbilical cord blood and maintaining cell culture for experimental analysis A guarantee of enough numbers of multipotent stem cells out of ... Materials and Methods Cell isolation and culture Canine umbilical cord blood (cUCB) and blood of the canine fetus heart using paracentesis was drawn and used for the isolation of mononuclear cells...

Ngày tải lên: 07/08/2014, 23:22

... plated in IMDM supplemented with 15% heat-inactivated fetal bovine serum and 100 UÆmL)1 penicillin ⁄ streptomycin and incubated at 37 °C in a humidified tissue culture incubator containing 5% ... washes and incubation with the nuclear stain 4¢,6-diamidino-2-phenylindone for 20 min, the MSCs were washed in NaCl ⁄ Pi for 10 and mounted in gelvatol for microscopic imaging Protein extraction and ... stimulatory signal in order to secrete IL-1b and TNF-a Hypoxia ⁄ SD induces the transcription and secretion of IL-10 Because of the lack of secretion of the in ammatory cytokines IL-1b and TNF-a from...

Ngày tải lên: 18/02/2014, 04:20

... llec gnimrofrep dna esu lacinilc rof SBF gnisu tuohtiw oviv xe sCSM dnapxe ot sdohtem dnif ot deen ew dna ,hcraeser ruo rof SBF desu ew ,yduts tneserp ni ,revewoH noitarefilorp sCSM rof noitidnoc ... 001-X notirT %4.0 neht dna ,nim rof enoteca %001 ,nim 01 rof lonahtem %001 dloc eci htiw detaert neht erew yehT erutarepmet moor ta nim 01 rof SBP ni edyhedlamrof %7.3 htiw dexif neht dna SBP ... noitcudni cinegoetso na desu ew ,sCSM eht morf noitaitnereffid cinegoetso ecudni oT laitnetop cinegoetso tibihxe sCSM doolb droC )A2 giF( nitnemiv dna 2HS snegitna detaler -myhcnesem rof ytivitisop...

Ngày tải lên: 07/08/2014, 18:21

... cells, increased in vivo reconstitution of hematopoietic lineages, and Wnt3a-specific maintenance of skin and intestinal stem cell populations [18] Because stem cells may share signaling mechanisms ... promoting MSC adipogenesis while repressing osteogenesis [52] The binding of PPARγ to various ligands, including longchain fatty acids and thiazolidinedione compounds, induces the transactivation and ... regulate MSC maintenance [19], as they in the selfrenewal of hematopoietic, neural, intestinal, and skin stem cells [18] Wnt3a treatment increases adult MSC proliferation while inhibiting their osteogenic...

Ngày tải lên: 09/08/2014, 10:20

... instructions Cells in which the nucleus was stained brown were defined as TUNEL-positive and the percentage of apoptotic cells per total number of cells was determined by two independent blinded investigators ... branches were ligated and excised The left hindlimb was kept intact and used as the nonischemic limb Simvastatin administration and MSCs transplantation Simvastatin administration and MSCs transplantation ... Center of Nanjing University (Nanjing, China) All animal experimental protocols were approved by the Animal Care and Use Committee of Nanjing Medical University and were in compliance with Guidelines...

Ngày tải lên: 10/08/2014, 05:21

... Figure Mesenchymal stem cells (MSCs) inhibit the proliferation of CD4 + T cells in vitro by induction of nitric oxide and prostaglandin E2 (PGE2) (a) CD4+ T cells, in the presence of accessory cells, ... development of collagen-induced arthritis Figure Mesenchymal stem cells (MSCs) inhibit the anti-CD3induced proliferation of CD4+ T cells in vitro (a) CD4+ T cells (5 × 104 cells) and accessory cells ... Carboxyfluorescein succinimidyl ester (CFSE)-labeled CD4+ T cells (5 × 104 cells) and accessory cells (5 × 104 cells) were incubated with μg/ml anti-CD3 antibody and the indicated numbers of mitomycin c-treated...

Ngày tải lên: 12/08/2014, 11:23

... enables infection of a wide variety of cells) have revealed differences in the efficiency of single round infection in cells of differing types and species [11,12] Therefore, to build upon and extend ... parental and mutant cells To examine if the defect in infection was in the early stages of the life-cycle we next examined the extent of integration of HIV-1 DNA after infection of parental and mutant ... and mutant cells and extent of HIV Growth Growth rates of parental and mutant cells and extent of HIV integration (A) Growth rates Parental and mutant 30-2 and 42-7 cells were seeded and growth...

Ngày tải lên: 13/08/2014, 05:22

... 2001;Function and interactions of integrins Cell Tissue Res (3):285-298 41 Mould AP, Askari JA, Aota SI, et al 1997;Defining the topology of integrin alpha5beta1-fibronectin interactions using inhibitory ... fibrin glue, mesenchymal stem cells and platelet-rich plasma Clin Oral Implants Res (5):579-586 51 Awad HA, Butler DL, Harris MT, et al 2000 ;In vitro characterization of mesenchymal stem cellseeded ... differentiation of human mesenchymal stem cells on laminin-5 J Cell Biochem (2):499-514 102 Lozito TP, Kuo CK, Taboas JM, et al 2009 ;Human mesenchymal stem cells express vascular cell phenotypes upon interaction...

Ngày tải lên: 11/09/2015, 09:16

... distinct functional domains, including an N-terminal domain, a coiledcoil domain, a DNA binding domain (DBD), a linker domain, an Src-homology (SH2) domain and a C-terminal transactivation domain ... tyrosine kinase JAK is inactive by binding to the intercellular domain of this gp130/LIFR complex Upon LIF binding, JAK kinase phosphorylates tyrosine sites at Y765/812/904/914 of gp130 and Y976/996/1023 ... POU domain are N-terminal and C- terminal domains that reveal little conservation The N-terminal domain (N-domian) is rich in proline and acidic residues while the C-terminal domain (C-domain) is...

Ngày tải lên: 14/09/2015, 13:38

... determined using the molybdenum-H2SO4 method (Schinttger et al., 1959) Protein was determined according to the method of Lowry et al (1951) using a standard of bovine serum albumin Nucleic acid and ... protein containing polysaccharide One milligram of the partially purified polymer contained 309.7 µg of protein, 160.2 µg of neutral sugar, 58.5 µg of hexosamine, 30.8 µg of uronic acid and 3.8 ... present (Stoveland and Lester, 1980; Lester, 1983) Various flocculating agents involving organic and inorganic compounds are widely used in wastewater treatment, dredging, and fermentation industries...

Ngày tải lên: 05/09/2013, 09:08

... II, P II and P IV) The A-chain of P II was formerly cloned and named recombinant pulchellin A-chain [15] The heterologous expression and refolding of a recombinant pulchellin binding chain was ... peptide chains of ricin, a toxic protein inhibiting protein synthesis Biochemistry 12, 3121–3126 21 Olsnes S & Pihl A (1973) Isolation and properties of abrin: a toxic protein inhibiting protein synthesis ... Pasqualucci L, Falini B & Stirpe F (1998) Evaluation of immunotoxins containing single-chain ribosome-inactivating proteins and an ati CD-22 monoclonal antibody (OM-124): in vitro and in vivo studies...

Ngày tải lên: 18/02/2014, 16:20

... Triangles and continuous line: data and fitting with PHI/PHO ratio of Asterisks and broken line: data and fitting with PHI/PHO ratio of hypothesis and therefore a direct involvement of PHI in electron ... explain the regulatory effect In fact, on addition of PHI, the fluorescence of PHR-bound flavin increases This finding points to a PHI-induced conformational change of PHR, possibly resulting in a ... degree of identity between the N-terminal sequence of PHI and those of ferredoxin-like proteins belonging to other oxygenases On the contrary, the N-terminus of PHI is identical to that of MopN...

Ngày tải lên: 21/02/2014, 00:20

... recently determined, has a membrane-spanning diheme containing subunit that binds a quinone molecule through the His ligand of heme b Asp, Gly, Met, Ala and the porfirine ring of heme b were also ... However, in the absence of boiling (Fig 1A, lanes and 4) high molecular mass bands of approximately 110 kDa and > 200 kDa were visible, as well as a faint band at 37 kDa, suggesting the presence of ... shows the SDS/PAGE of purified ccNiR upon different treatments The complex dissociates into an intense band of 61 kDa (NrfA) and a band of weak intensity of 19 kDa (NrfH), confirming its hetero-oligomeric...

Ngày tải lên: 21/02/2014, 00:20

... circle (OC) and supercoiled (S) plasmid DNA are indicated; lanes 2±3 containing 20 or lM Ch-Prx1 protein show that protection of DNA against nicking increases with increasing amounts of protein; lane ... corresponding to the 3¢ region including the polyA tail, plus bp in the 5¢ region Screening of a Chlamydomonas genomic BAC library (Genome Systems Inc., St Louis, USA) resulted in the isolation of a ... dif®culty, thioredoxin-de®cient yeast or E coli mutant strains have been used to express a single-cysteine mutant thioredoxin allowing the isolation of thioredoxin targets in yeast [8] and Fig Ch-Prx1...

Ngày tải lên: 08/03/2014, 16:20

... a,b-elimination reaction of d-cysteine and of several d-cysteine derivatives, and also the formation of d-cysteine or d-cysteine-related amino acids from b-chloro-d-alanine in the presence of various ... methionine and excluding the terminating amino acid The protein has a predicted molecular mass of 43.9 kDa and a pI of 7.2 It contains relatively high amounts of the sulfur amino acids cysteine (four ... probability of occurrence Enzyme activity of the recombinant protein The recombinant Arabidopsis d-CDes proteins including and excluding the targeting peptide were expressed in E coli and already...

Ngày tải lên: 16/03/2014, 18:20

... expression and use in in vivo targeting applications Specifically, we asked whether binding affinity was retained in the absence of the conserved immunoglobulin intradomain disulphide bond In an initial ... series of experiments, recombinant 12F-11 protein was denatured and reduced using guanidine HCl and dithiothreitol, followed by alkylation of the cysteine residues and refolding However, in a result ... mutant (12F-11DCys) Use of alanine and valine was initially determined in a set of competitive selection experiments [35], and replacement with this pair of residues maintains a hydrophobic character...

Ngày tải lên: 17/03/2014, 10:20

... following toxin-mediated damage in vivo Percentage depurination was determined by quantifying the intensity of the liberated fragment in relation to the remaining intact 25S rRNA plus fragment using ... seen in this yeast strain genuinely reflects of the sensitivity of the mutant toxin to temperature To obtain a clearer picture of the growth profiles of yeast cells expressing these RTAs, cells ... strains of yeast, in a manner akin to its expression in plant cells [18] Survivors of toxin expression may contain defects in genes normally essential for toxin retro-translocation, refolding,...

Ngày tải lên: 23/03/2014, 07:20

... epithelial cells of the mammary gland Likewise, the deduced amino-acid sequences of both bovine and human MUC15 resemble the mucins in having serine, threonine and proline as the predominant amino acids, ... PCR-screening of the bovine mammary gland cDNA library The N-terminal amino-acid sequencing of purified bovine MUC15 revealed Glu24 as the initial residue of the mature protein, showing that the preceding ... mucin-like glycoprotein, MUC15, and cloning of a human homologue The mature protein contains a single transmembrane domain, and is proposed to be oriented with a small intracellular C-terminal...

Ngày tải lên: 24/03/2014, 04:21

... the generation of deletion mutants containing discrete functional domains of XHIVEP1 Thus, the cloning and characterization of the XHIVEP interacting factors would be of interest and should also ... proteins Black bars indicate the position of the ZAS C2H2 zinc fingers (ZAS-N and ZASC) and the isolated C2HC zinc finger (I-Znf) The ZAS zinc finger DNA binding domains are followed by acidic domains ... are shown in comparison with the corresponding domains from human (hs), mouse (mm), rat (rn) and fly (dm) Amino acids involved in complex formation with zinc ions are marked in bold and amino acids...

Ngày tải lên: 30/03/2014, 13:20

... a peak increase of approximately 53% in the number of CD34 expressing cells and and a 90% increase in CD133 cells in circulation Furthermore, a significant augmentation of cells possessing hematopoietic ... required to ingest two capsules of Stem- Kine in the morning and two in the evening for 14 days Phenotypic assessment of circulating stem cells Peripheral blood mononuclear cells (PBMC) were isolated ... marrow injury conditions [43], subsequent studies have expanded this finding to situations of inflammation [44], and peripheral tissue injury [45-47] Hematopoietic stem cells are being increasingly...

Ngày tải lên: 18/06/2014, 16:20