isolation and characterization of the microprocessor complex

... of author information is available at the end of the article and chrysanthemum (Chrysanthemum × morifolium) In other floricultural plants, the development of doubleflowered varieties is one of ... conferred by the polyacylated anthocyanin gentiodelphin [24] The flavonoids of Japanese gentian, the structures of the anthocyanins and flavones, and the biosynthetic structural and regulatory ... long, and consisted of nine exons and eight introns (Fig 5a) The number and position of introns were conserved between Arabidopsis AG and GsAG1 The second and third introns of GsAG1 (4.3 kb and

Ngày tải lên: 26/05/2020, 21:46

... sec and it was used for the calculation of the viscosity of the broth Sugar was estimated by phenol sulphuric acid method using glucose as a standard By measuring the viscosity of the broth the ... The medium was inoculated with (v/v%) of the inoculum and incubated at 37°C for 72 hrs at 200 rpm The different isolates were inoculated in these media and after incubation the viscosity of the ... of standard xanthan and produced xanthan of SC1 and SC4 showed that the most important bands recorded were: 3400-3450 cm-1: axial deformation of -OH; 2850-2950 cm1: axial deformation of C-H and

Ngày tải lên: 13/01/2020, 23:19

... observed Figure shows the effect of the concentration of the polymer on the flocculation of kaolin clay When the polymer concentration was changed from zero to 12.5 ppm, the extent of flocculation ... (Tris-HCl) buffer of pH 8.0 Elution was done with the same buffer at a flow rate of ml/h and ml fractions were collected The void volume of the column was determined by the elution volume of Blue Dextran ... follows: 500 g of soil was washed times with distilled water and suspended in 1,000 ml of water The mixture was allowed to stand for 10min and the supernatant was collected and used for the flocculating

Ngày tải lên: 05/09/2013, 09:08

... polymerization of styrene and the blending of PS and SPS polymers have affected the surface structure of the PVDF membrane obviously The cross-section morphologies of the PVDF membrane, the PVDF-PS ... Thermal analysis of the PVDF-SPS composite membrane The TG analysis curve is presented in Figure for the investigation of the thermal stability of the PVDF membrane, PVDF-PS membrane, and the PVDF-SPS ... to prepare the PVDF composite membrane The structure and the morphology of the PVDF-SPS composite membrane were characterized The proton conductivity and the methanol permeability of the PVDF-SPS

Ngày tải lên: 05/09/2013, 16:11

... from the s equences of N- and C-terminal peptides (P1 and P10) were us ed as primers to s ynthesize t he cDNA of the coding sequence of t he mature Prx. The direct P CR primers were synthesized ... characteristics of Ch-PRX1 The amino-acid sequence of Ch-Prx1 shares highest identity with the BAS1 protein of Brassica, spinach, barley and A. thaliana,PR1ofPhaseolus and MHF 9 of A. thaliana. These proteins ... components (Ch-Prx1, Trx h and NTR). The speci®c activity of the recombinant e nzyme w as identical to the speci®c activity of the native protein puri®ed fro m Chlamydomonas (dat a not shown). The rate of NADPH

Ngày tải lên: 08/03/2014, 16:20

... obtained and studied but their analysis showed that they did not contain the central region or the 5¢ end of the gene. The cosmid clone containing the longest part of the new gene was named CAR1 and ... mapping experiments. The last exon is composed of a coding sequence of 193 bp and of an untranslated region of at least 131 bp. The size of the 24 introns ranges from 79 bp to about 16 kb. The size of the largest ... good degree of sequence identity (67%) between the two Fig. 2. Alignment of the amino-acid sequences of the Muc4 repeats (A) and comparison of the repeat consensus sequences of Muc4 and SMC (B). (A)

Ngày tải lên: 08/03/2014, 23:20

... to the presence of d-cysteine in the culture medium. It was suggested that d-cysteine exerts its toxicity through an inhibition of threonine deaminase. On the other hand, the presence of the ... cycle and the parts above ground were harvested every 4 h and frozen in liquid nitrogen. The d-CDes mRNA levels increased at the end of the light period, reached a maximum at the end of the dark ... and decreased at the beginning of the light cycle. The d-CDes gene expression or the stability of the d-CDes mRNA was negatively regulated by light (Fig. 6A). The Western blot results using the

Ngày tải lên: 16/03/2014, 18:20

... function of the two isoforms, prompted us to analyze the organiza- tion and the promoter sequences (Fig. 4) of the two tryptase genes. The length of both genes, as evaluated from the size of the PCR ... agarose gel electrophoresis and the bands eluted, purified and subcloned in the PCRII TM TOPO vector containing the lac promoter and the b-galactosidase gene, using the TA Cloning Kit (Invitrogen, ... in the two tryptases and the results confirm once again the presence of Asn188- Phe189 in BLCT, unlike BLT and tryptases from other species which contain Asp-Ser in those positions. Isolation and

Ngày tải lên: 17/03/2014, 09:20

... signals recorded in the spectra were mapped onto the anticipated sequence of subunit C on the basis of their mass value and the specificity of the enzyme, leading to the complete verification of the amino ... parallel the effect of the absence of other homologous regulatory subunits of oxygenase complexes, like T4MOD [33] and component B of methane monooxygenases [38,39]. In these cases the absence of the ... in Materials and methods. Quantitative analysis of bound FAD yielded the value of 1.1–1.2 mol of FAD per mole of protein. TheironcontentofTomoFwas1.8–2.1molÆmol )1 of protein, and the acid-labile

Ngày tải lên: 17/03/2014, 10:20

... role for these residues on the catalysis and zinc ions, respectively. These effects may be necessary either for the activation of the catalytic water molecule or for the stabilization of the substrate–enzyme ... activity [33]. These results indicate the importance of these residues in the stability of di-zinc binding and suggest that these residues are essential for the enzymatic activity of the PepD. To ... content of the purified PepD wild- type and mutant proteins. Loss of enzymatic activity in the mutants was not due to changes in the second- ary structure of the proteins because the CD spectra of the

Ngày tải lên: 23/03/2014, 06:20

... revealed the codon usage of the Arabidopsis gene as well as the nature of the signal peptide and the sequence motif around the translation start as critical parameters for efficient expression of AtSMT ... secretion and may even outperform the impact of SRP-dependence. Interestingly, C-terminal extension of the SMT sequence with both the ER retention sig- nal and the 6xHis tag led to severe reduction of ... mechanism. Investigation of the substrate specificity of AtSMT towards l-malate revealed structural features required for the interaction of the acyl acceptor with the enzyme. The lack of enzymatic activity

Ngày tải lên: 23/03/2014, 07:20

... Gly-Ser, in-frame with the codon for Pro2 of the receptor cDNA The codon for the last used amino acid of the receptor (Ser412 in case of the full-length receptor and Ala316 for the truncated receptor) ... concentrations of 10±20 times their Ki value Without ligand, a half-life of 33 days was obtained This increased to 77 days in the presence of NECA (agonist) and 53 days in the presence of theophylline ... temperature and using high concentrations of the weak antagonist theophylline combined with low ¯ow rates The ®nal ionexchange step allowed removal of theophylline, and a reduction of volume and detergent

Ngày tải lên: 24/03/2014, 00:21

... combinations of degenerate and specific primers and by rescreening the cDNA libraries The partial clones of XHIVEP2 and -3 covered 5.9 and 3.2 kb of the respective 3¢ ends and included 3¢-UTRs and poly(A)-tails ... representation of the full length XHIVEP1 and the Myc-tagged (MT) deletion mutants Black and white boxes indicate the position of the Znf domains and the serine-rich stripe, respectively The relative ... bold The position of the terminal amino acid for the depicted sequence is indicated to the right (Fig 6B, compare lanes and with and 6) Mutation of the GAGA motif only slightly altered the ZAS-N...

Ngày tải lên: 30/03/2014, 13:20

... validation of their structure and characterization of their functions Even after the identification, the next would be to understand how the molecular components of the cells are controlled, interact and ... third of the human genome consists of repetitive sequences Almost all of these have arisen by retroposition of an RNA intermediate followed by insertion of the resulting cDNA into the genome Of these, ... process of integration, the viral genome breaks at E1 and E2 regions, never at the E6 and E7 region The loss of 19 Literature Survey E2 results in the loss of E6 and E7 regulation This allows the...

Ngày tải lên: 16/09/2015, 17:13

... et al of the ndh genes is much higher in BS chloroplasts, and elevated amounts of the Ndh complex have been found in these plastids [18] The function of the Ndh complex is still a matter of debate ... for the Ndh membrane subcomplex The Ndh antibodies recognized the 46, 28, 18 and 12 kDa polypeptides, corresponding to the NdhH, -K, -J and -E subunits of the Ndh complex The intact Ndh complex, ... characterize the Ndh complex, the MS and BS chloroplasts were separated on CN ⁄ PAGE (1D, based on the internal charge of the protein complexes) and BN ⁄ PAGE (2D, based on the external charge of the...

Ngày tải lên: 23/03/2014, 13:20

... correlations The determination of the 3D structure of D desulfuricans ATCC 27774 NrfA [17] enabled to ascertain the spatial characterization of the five hemes, namely their proximity and the axial ... consequence, the stoichiometry of the complex was not established The formation of stable NrfHA complexes in the e-proteobacteria and especially in the d-subdivision members should be advantageous to the ... containing the double of NrfA subunit in respect to the NrfH subunit Nevertheless, inside these huge aggregates there are species of the type a2b2 The present findings are in agreement with the reported...

Ngày tải lên: 21/02/2014, 00:20

... alignments and for CDRs and In these cases extensive loop modelling was undertaken and the final model selection based on the modeller objective score Construction of the 12F-11DCys mutant and 12F-11 ... background, and all of these proved to be identical sequences One of these, designated clone 12F-11, was chosen for further analysis The primary and deduced amino acid sequences of clone 12F-11 ... underlined, and the alanine linker and dual octapeptide FLAG tags are italicised The positions of the CDR1 and )3 regions are indicated in bold type (B) Alignment of protein 12F-11 with four other VNAR...

Ngày tải lên: 17/03/2014, 10:20

... diagram of wild-type RTA structure, and the positions of the altered amino acids of the double mutant, F108 and L151, within the structure are indicated Discussion RTA is the catalytic polypeptide of ... plasmid DNA containing the 25S rRNA genomic DNA sequence The probe was made by random priming and the incorporation of [a-32P]dCTP Hybridization of the probe to the membrane and subsequent washes ... Tsurugi K (1987) The mechanism of action of ricin and related toxic lectins on eukaryotic ribosomes The site and the characteristics of the modification in 28 S ribosomal RNA caused by the toxins J...

Ngày tải lên: 23/03/2014, 07:20

... species B the intensity of the a band (556 nm) was greater than that of the b band (530 nm) This spectral feature is similar to that of oxyferrous complexes of P450s, particularly at the Q band region ... diffusion of the substrate We also estimated the enzyme activities of aqueous PGIS and encapsulated PGIS using the same concentration of guaiacol and peracetic acid The initial rates of the aqueous and ... sample showed the features of the resting enzyme (i.e a Soret peak at 418 nm and discrete a bands and b bands at 570 and 537 nm, respectively (data not shown), whereas loss of < 5% of the original...

Ngày tải lên: 23/03/2014, 07:20

... This result in the successive generation of two active forms of the factor: aFXIIa and bFXIIa The cleavage of the Arg353-Val354 bond of FXII results in the formation of aFXIIa form of the enzyme, ... intrinsic tenase and the prothrombinase complexes, respectively The combined actions of the intrinsic and extrinsic tenase and the prothrombinase complexes lead to an explosive burst of the enzyme ... combined with the cofactor thrombomodulin in the protein Case complex The product of the protein Case reaction, APC, inactivates the cofactors FVa and FVIIIa The cleaved species, FVai and FVIIIai,...

Ngày tải lên: 14/09/2015, 13:36