isolation and characterization of human prostate stem progenitor cells

... and mutant cells and extent of HIV Growth Growth rates of parental and mutant cells and extent of HIV integration (A) Growth rates Parental and mutant 30-2 and 42-7 cells were seeded and growth ... x 105 Figure cells with an HIV-EGFP vector at a moi = 0.5 Infection of parental and mutant HeLa cells (30-2 and 42-7) Infection of parental and mutant HeLa cells (30-2 and 42-7) cells with an ... fractionation of cytoplasmic and nuclear extracts and measurement of the amount of full product 36 hours after infection in parental (black bars) and 42-7 (grey bars) cells; (E) Parental and 42-7 cells...

Ngày tải lên: 13/08/2014, 05:22

... Isolation of HPTC and characterization of isolated and commercial HPTC 50 4.1.1 Isolation of HPTC 50 4.1.2 Characterization of HPTC by immunoblotting 51 4.1.3 Characterization of ... containing 0.1 mM of 3-isobutyl-1-methylxanthine (IBMX) (Merck) and exposure of cells to 100 nM and 1000 nM of human PTH (Sigma-Aldrich) and human AVP (SigmaAldrich) for 30 minutes at 37°C The cells were ... (p-values of < 0.05) unless stated otherwise 49 Results 4.1 Isolation of HPTC and characterization of freshly isolated and commercial HPTC 4.1.1 Isolation of HPTC HPTC are available commercially and...

Ngày tải lên: 09/09/2015, 10:19

... Import and assembly of proteins into mitochondria of mammalian cells Biochim Biophys Acta 1592, 97–105 19 Suzuki, H., Maeda, M & Mihara, K (2002) Characterization of rat TOM70 as a receptor of the ... respectively, and the resultant VNARs evaluated for retention of binding affinity Material and methods Equipment and reagents Restriction enzymes and Vent DNA polymerase were purchased from New England ... ampicillin and/ or 2% (w/v) glucose Solid media contained 2% (w/v) Bacto-agar Transformation of E coli was by standard procedures [24] performed using electrocompetent cells Isolation of total RNA...

Ngày tải lên: 17/03/2014, 10:20

... (Figs 4E and F) Discussion Isolation and characterization of stem cells derived from various tissues and sources have been one very critical issue for stem cell therapy [12,28,33] The purpose of this ... capacities Stem Cells Dev 2007, 16, 597-604 14 Igura K, Zhang X, Takahashi K, Mitsuru A, Yamaguchi S, Takashi TA Isolation and characterization of mesenchymal progenitor cells from chorionic villi of human ... Cotransplant of neural stem cells and NT-3 gene modified Schwann cells promote the recovery of transected spinal cord injury Spinal Cord 2007, 45, 15-24 12 Hipp J, Atala A Sources of stem cells for...

Ngày tải lên: 07/08/2014, 23:22

... potency of stem cells 1.1.2 Fundermental features of stem cells 1.1.3 Signalling pathways modulating pluripotency in stem cells 1.1.4 Transcription factors controlling pluripotency of stem cells ... unspecialized daughter cells and the potential of differentiation into specialized cells Stemness refers to the undifferentiated status of self-renewing stem cells Stem cells show a hierarchy of developmental ... mammalian systems xii Chapter I Introduction Chapter I Introduction 1.1 Stem cells 1.1.1 Developmental potency of stem cells Stem cells are unspecialized cells that have the capability of self-renewal...

Ngày tải lên: 14/09/2015, 13:38

... cancers and prostate cancers, and is also the candidate site of potential oncogene(s) other than c-myc located at 8q24.1 Analysis of paired biopsies of invasive ductal breast cancer and adjacent ... study of isolation and characterization of a novel human gene was initiated The objectives of this study were as follow: To isolated full length cDNA; To analyze the genomic structure of MOST-1; ... protein in human cell lines namely hTERT-HME1 normal human mammary epithelial, MCF7 breast adenocarcinoma, PrEC normal human prostate epithelial and DU145 prostate carcinoma Aggregation of overexpressed...

Ngày tải lên: 16/09/2015, 17:13

... polysaccharide One milligram of the partially purified polymer contained 309.7 µg of protein, 160.2 µg of neutral sugar, 58.5 µg of hexosamine, 30.8 µg of uronic acid and 3.8 µg of phosphorus Constituent ... concentration of ppm to estimate the effect of pH After min, A660 was measured and the extent of flocculation was calculated Pronase digestion of extracellular polymer Forty milligrams of the extracellular ... as follows: 500 g of soil was washed times with distilled water and suspended in 1,000 ml of water The mixture was allowed to stand for 10min and the supernatant was collected and used for the...

Ngày tải lên: 05/09/2013, 09:08

... Nomenclature of the toxic pulchellin lectins The abbreviation P is followed by the Roman numerals I, II, III and IV and refers to each pulchellin Characterization of four pulchellin isoforms isoform ... III and P IV isoforms from the eluate P III ⁄ P IV (Fig 1C) Isoelectric focusing gave pI of 5.8, 5.7, 5.5 and 5.2 for the four isoforms respectively Secondary structure of the pulchellin isoforms ... mixture of other isoforms (B) Gel visualization of proteins eluted Lane 1, molecular weight markers Numbers on the left indicate the Mr values of the standards in thousands Lanes 2–9, lg of peak...

Ngày tải lên: 18/02/2014, 16:20

... of 50 nmÆmin)1, band-width of 1.0 nm, and a resolution of 0.1 nm in the temperature range between 10 °C and 70 °C, after preincubation for 10 at each temperature Three runs were accumulated and ... of 0.6 lM The data were fitted to Michaelis–Menten curves Squares and dotted line: data and fitting with PHI/PHO ratio of 0.5 Triangles and continuous line: data and fitting with PHI/PHO ratio of ... Asterisks and broken line: data and fitting with PHI/PHO ratio of hypothesis and therefore a direct involvement of PHI in electron transfer This conclusion is consistent with the very low degree of identity...

Ngày tải lên: 21/02/2014, 00:20

... intense band of 61 kDa (NrfA) and a band of weak intensity of 19 kDa (NrfH), confirming its hetero-oligomeric nature (Fig 1, lane 1) However, in the absence of boiling (Fig 1A, lanes and 4) high ... and 4) high molecular mass bands of approximately 110 kDa and > 200 kDa were visible, as well as a faint band at 37 kDa, suggesting the presence of dimers All of the bands stained positively for ... suggests that the bands at approximately 110 and 37 kDa correspond to strongly attached dimers of both NrfA (a2) and NrfH (b2), respectively The presence of dithiothreitol (Fig 1A, lanes and 2) did not...

Ngày tải lên: 21/02/2014, 00:20

... backbone amide and carbonyl of Cys140 form hydrogen bonds with the backbone carbonyl and amide of Trp123, the side chain of which forms one wall of the active site cavity and the replacement of which ... site-directed mutagenesis, and the parent Q143A hMnSOD and wild-type hMnSOD, are given in Table and Fig Catalysis of the decay of superoxide by the single mutants of Table all followed Michaelis ... the uncatalyzed dismutation of superoxide [32] A least-square fit of Eqn (1) to the data of Fig yields the constants given in the legend of Fig The values of kcat ⁄ Km and kcat for catalysis by...

Ngày tải lên: 07/03/2014, 11:20

... Chlamydomonas cells Chlamydomonas CW15 cells were grown to a cell density > ´ 106 cells per mL Cells were pelleted, resuspended in 30 mM Tris/HCl pH 7.9, and broken by two cycles of freeze-thawing ... RESULTS Cloning and sequences of Ch-Prx1 cDNA and the Ch-Prx1 gene In order to complete the sequence data for this new protein, and to be able to make a thorough characterization of its biochemical ... BLAST of peptide sequences of 21 kDa proteins in databases Tryptic peptides were puri®ed by RP-HPLC and some of them were totally or partially analyzed by Edman sequencing (ááá) and/ or by MALDI-TOF...

Ngày tải lên: 08/03/2014, 16:20

... presequence of 19 amino acids The mature protein would have a molecular mass of 41.7 kDa and a pI of 6.34 The YedO protein from E coli and the d-CDes from Arabidopsis showed an overall identity of 36% and ... d-CDes mRNA and protein levels in older plants, during the light phase in a diurnal light ⁄ dark rhythm and under sulfate limitation Results In silico characterization and isolation of the Arabidopsis ... purity of mitochondria prepared from green tissue of tobacco, pea and Arabidopsis thaliana Phyton 43, 215–229 53 Leegood RC & Malkin R (1986) Isolation of sub-cellular photosynthetic systems In...

Ngày tải lên: 16/03/2014, 18:20

... strain genuinely reflects of the sensitivity of the mutant toxin to temperature To obtain a clearer picture of the growth profiles of yeast cells expressing these RTAs, cells were plated at various ... permissive temperatures of 30 °C and 37 °C, respectively A sample of the cells was removed from each culture, rRNAs were isolated in TRIzolÒ (Invitrogen, Paisley, Scotland), and the extent to which ... site molecules Y80, Y123 and E177 are shown in green and the position of the two mutated amino acids, F108 and L151, are shown in blue between the aromatic rings of Tyr80 and Tyr123 close to the...

Ngày tải lên: 23/03/2014, 07:20

... tonsil, and fetal liver Furthermore, PCR screening of bovine and human cDNA libraries showed the presence of MUC15 mRNA in human breast, bovine mammary gland, bovine lymph nodes and lungs of both ... Identification and cloning of the human MUC15 cDNA In order to investigate the existence of a human MUC15 homologue, the bovine MUC15 nucleotide sequence was employed in a search of the human genome ... database, and a similar sequence was located The milk cell fraction of lactating tissue contains bud-off epithelial cells, enabling performance of an indirect assay for expression of this possible human...

Ngày tải lên: 24/03/2014, 04:21

... of degenerate and specific primers and by rescreening the cDNA libraries The partial clones of XHIVEP2 and -3 covered 5.9 and 3.2 kb of the respective 3¢ ends and included 3¢-UTRs and poly(A)-tails ... nucleus after transfection of COS cells [23,24], and the endogenous human HIVEP (PRDII-BF1) protein was detected in the nucleus of MG63 cells [6] Visual inspection of the full length XHIVEP1 ... -2 and -3) The Xenopus XHIVEPs are characterized with respect to temporal and spatial expression, nuclear import/export activity and DNA binding specificity Materials and methods Isolation and...

Ngày tải lên: 30/03/2014, 13:20

... streptomycin and non-essential amino acids Vero Cells were obtained from ATCC and were maintained in MEM with Earl's salts and the addition of 10% FBS and standard concentrations of L-glutamine, ... Domains of E9 and locations of mutations responsible for drug resistance Domains of E9 and locations of mutations responsible for drug resistance The location of the putative exonuclease and polymerase ... Mapping and marker rescue of recombinant viruses A Map of PCR fragments in the E9L region that were used for marker rescue mapping experiments and reconstruction of resistant viruses B Results of...

Ngày tải lên: 20/06/2014, 01:20

... the G and P serotype specificities of the YK-1 strain, the sequences of the genes encoding both VP7 and VP4 were determined and compared with those of representatives of established G and P serotypes ... Electrophoretic study of the genome of human rotaviruses from Rio de Janeiro, Sao Paulo and Belem, Brazil J of Hygiene 1983, 90:117-125 Palombo EA, Bishop RF: Genetic and antigenic characterization of a serotype ... rotavirus RNA segment 11, and (c) schematic diagram of the sequences of gene segment 11 Arrows indicate segment 11 of RRV and YK-1, or rearranged segment 11 of variant 311 The YK-1 and its variant clone...

Ngày tải lên: 20/06/2014, 01:20

... eht fo esahp suoeuqa reppu eht no detcelloc saw AND lairetcaB nim rof 000,6 ta degufirtnec dna nim rof sllec lairetcab gniliob yb detcartxe neht saw AND lairetcab ;retaw dellitsid elbuod dezilirets ... 817( rof evitisop ,9~4 senal ;)pb 001,1( rof evitisop ,3 dna senal ;noitcefni laccocotperts rof evitagen ,1 enal ;lortnoc evitagen ,N enal ;)pb 003 ,M4040 ( lortnoc evitisop ,P senal ;reddal AND ... enilav ,esapil rof snoitcaer evitagen dah setalosi llA nispyrtomyhc -α dna esatahpsohp enilakla rof snoitcaer evitisop dah fo setalosi 81 dna esadinimasoculg-β-lyteca -N dna esaretse rof snoitcaer...

Ngày tải lên: 07/08/2014, 18:21

... of human erythrocytes were dispensed to Figure Phylogenetic analyses of HA and NA of an equine H5N1 isolate sequence in comparison to Egyptian human and avian isolates a, HA gene b, NA gene Human ... poultry increase exposure risks to surrounding humans and other mammals and in turn, create opportunities for the emergence of human- adapted strains with pandemic potential [19,20] Since 2006, H5N1 ... letters showed site of H274Y and N294S substitutions (H275Y and N295S in N1 influenza numbering) cough, fever and serous nasal discharge The course of the disease was short (72 h) and responsive well...

Ngày tải lên: 10/08/2014, 05:21