Molecular mechanisms involved in the specification of embryonic ectoderm in the zebrafish embryo

... (Dsh) The amino-terminal extracellular Wnt binding domain and the 18 19 carboxy-terminal PDZ-binding domain of the Fz protein are known to be essential for Fz function Deletion of the PDZ-binding ... In this thesis, we use the zebrafish as a model to study the molecular mechanisms behind the specification of embryonic ectoderm Specifically, we attempt to isol...

Ngày tải lên: 16/09/2015, 17:12

... implicated in INHR clinical Mtb isolates as well, although their roles in INH resistance remains unascertained in a large proportion of these genes (119) Approximately 10-25% of INHR strains not contain ... isolate Regardless, the numerous handling and in vitro passages of these individual strains in various labs could translate into the acquirement of stable mutations...

Ngày tải lên: 09/09/2015, 08:14

... comparing results across studies and obtaining a global overview of the disease pathogenesis In this paper, we perform a transcriptomebased study by combining the analysis of co-expressed gene networks ... genomewide motif analysis [4] of the genes in the disease- associated module revealed several cis-regulatory elements that matched to the binding sites of transcr...

Ngày tải lên: 14/08/2014, 21:20

... A FUNCTIONAL GENOMICS APPROACH FOR ELUCIDATION OF NOVEL MECHANISMS INVOLVED IN GnRH REGULATION OF THE GONADOTROPINS By LUO MIN (B SC.) A THESIS SUBMITTED FOR THE DEGREE OF DOCTOR OF PHILOSOPHY ... regulate the synthesis of the gonadotropins directly at the pituitary or indirectly at the hypothalamus by modulating GnRH secretion The gon...

Ngày tải lên: 11/09/2015, 21:47

... Phosphoinositide Pathway 1. 10 Aim of Thesis 6 7 9 10 11 12 13 14 16 16 19 19 19 21 21 26 26 28 28 30 31 32 ii Chapter Materials and Methods 2 .1 2.2 2.3 2.4 2.5 2.6 2.7 2.8 2.9 2 .10 2 .11 2 .12 2 .13 ... Hedgehog 1. 3.4 Regulation of Smoothened Activity 1. 4 Activation of Hedgehog protein 1. 5 Comparing Hedgehog Orthologs 1. 5 .1 Patched 1. 5.2 Smoothened...

Ngày tải lên: 10/09/2015, 15:49

... kinesin-like protein of 1363 amino acids was assembled Like Drosophila Cos2 and other members of the kinesin heavy chain (KHC) superfamily of proteins, the N-terminal and C-terminal regions of ... about the mechanism of the pathway The aim of this study focuses on two proteins, Cos2 /Kif7 and Iguana /Dzip1 Drosophila Cos2 plays an important role in Hh signal...

Ngày tải lên: 10/09/2015, 15:49

... multiplicity testing by controlling the false discovery rate [27] The differences between the transcriptional profiles induced by the six toxicants were less prominent in the datasets from the 4-24 hpf ... numbers of embryos and can thus average the individual gene responses by using pooled cDNA prepared from many embryos In the cases where we confirmed the gene res...

Ngày tải lên: 14/08/2014, 08:20

... EFFECTS OF HIGH GLUCOSE CONCENTRATIONS ON THE EXPRESSION OF GENES INVOLVED IN PROLIFERATION AND CELL- FATE SPECIFICATION OF MOUSE EMBRYONIC NEURAL STEM CELLS FU JIANG (MD, MMed) A THESIS ... Illustration Schematic summary of the effects of high glucose on the expression of developmental control genes that are involved in...

Ngày tải lên: 30/09/2015, 06:36

... hypersensitivity-related proteins of Arabidopsis and tobacco; and (b) acyl transferases such as anthranilate N-hydroxy-cinnamoyl/benzoyl-transferase (NHCBT)-like protein of Arabidopsis and Dianthus caryophyllus, ... strawberry [9] and yeast AATs [33] CMAAT1 was capable of accepting branched alcohols such as 2- and 3-methylbutyl alcohol (also named amyl and isoamyl alcohols...

Ngày tải lên: 31/03/2014, 21:21

... as: Qin and Tian: Exploring the molecular mechanisms underlying the potentiation of exogenous growth hormone on alcoholinduced fatty liver diseases in mice Journal of Translational Medicine 2010 ... mRNA expression HMW adiponectin is the major bioactive isoform of adiponectin, and is responsible for the insulin-sensitizing effects of adiponectin, whil...

Ngày tải lên: 18/06/2014, 16:20

... antithrombins are glycosylated at four of their asparagine molecules; these are of the α isoform The remaining 5–15% of circulating antithrombins, of the β isoform, lack glycosylation at asparagine ... purposes) Interaction of antithrombin with endothelium The affinity of antithrombin (AT) to thrombin and its enzymatic inhibition is increased by binding of AT with t...

Ngày tải lên: 12/08/2014, 23:21

... the molecular mechanisms underlying latency in the monocyte-macrophage lineage is poor Like in CD4+ T cells, two types of latency occur in cells from the monocyte-macrophage lineage Page of 16 ... cells of the monocyte-macrophage lineage However, several mechanisms generating HIV-1 post-integration latency have been described in the monocyte-macrophage,...

Ngày tải lên: 12/08/2014, 23:23

... 2. 1 ES cell secretion of LEFTY2 maintains pluripotency 2. 1.1 Introduction The molecular basis of self- renewal and the maintenance of pluripotency in ES cells have yet to be ... secreted into and deposited in the ES cell microenvironment 79 2. 1 .2. 8 LEFTY2 maintains ES cell self renewal by inhibiting Nodal signaling Next, the mechanism of LEFT...

Ngày tải lên: 11/09/2015, 16:02

... signals dictate embryonic stem cell fates is also not entirely clear Conflicting roles of Nodal signaling in both the maintenance of ES cell self renewal and induction of ES cell differentiation ... be learnt The primary aim of this project hence serves to identify and delineate the roles of additional factors and pathways that are important for the...

Ngày tải lên: 11/09/2015, 16:02

... to fixing in 4% formaldehyde for 1min Before the stain solution was added, the cells were again rinsed thrice with PBS to remove the fixative The samples were then incubated for 15min in the dark ... induction, the drug was removed from the cells by changing the culture medium The E14tg2a cells were fed daily with fresh medium containing no Doxycyclin 4. 14. 1.3 Treatm...

Ngày tải lên: 11/09/2015, 16:02