molecular mechanisms involved in chromatin dependent inheritance
Characterization of the molecular mechanisms involved in ethionamide activation in mycobacteria
... wall proteins, lipoproteins and secretion systems; 3, macrophage-interacting proteins; 4, protein kinases; 5, proteases; 6, metal-transporter proteins; 7, gene expression regulators including two ... roles in INH resistance remains unascertained in a large proportion of these genes (119) Approximately 10-25% of INHR strains not contain mutations in any of the known gene targets for INH resistance, ... actinobacteria, mycolic acids consisting of βhydroxyl fatty acids with long α-alkyl side chains line the inner layer of the mycomembrane, covalently linked to arabinogalactan in the standard inner...
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Molecular mechanisms involved in the specification of embryonic ectoderm in the zebrafish embryo
... amino-terminal extracellular Wnt binding domain and the 18 19 carboxy-terminal PDZ-binding domain of the Fz protein are known to be essential for Fz function Deletion of the PDZ-binding domain ... GSK3β is a serine/threonine kinase involved in insulin, growth factor and Wnt signalling In the absence of Wnt signalling, GSK3β is recruited to the APC complex via interaction with Axin, where it ... Lin, 2004) Recently, it was demonstrated that Axin also plays an important role in a JNK signalling pathway by utilizing discriminatory domains for its distinct roles in the both the β-catenin...
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Báo cáo khoa học: Molecular mechanisms of the phospho-dependent prolyl cis ⁄ trans isomerase Pin1 docx
... colony-stimulating factor [80], interleukin-2 and interferon-c [81] The ARE-containing cytokine mRNAs interact with AUF1 factors, and this interaction targets them for degradation Pin1 interferes ... catalytic domain could induce structural changes in the N-terminal proline-rich domain of IRF-4 In the same field of immunology, Pin1 also regulates the production of such proinflammatory cytokines as ... novel interaction surfaces for two distinct molecular partners [6], and the conformation of a proline in the linker region between two SH3 domains of the Crp adaptor protein determines the autoinhibition...
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Báo cáo khoa học: Two functionally redundant isoforms of Drosophila melanogaster eukaryotic initiation factor 4B are involved in cap-dependent translation, cell survival, and proliferation doc
... Dm-eIF4B-L in Drosophila cell cultures and in developing tissues provided interesting insights into the function of eIF4B In mammalian cell cultures, a 50-fold overexpression of eIF4B resulted in the inhibition ... obtained for the first cistron in the absence of cap inhibitor This indicated that the Ubx IRES is indeed driving internal initiation of the second cistron in our in vitro translational system ... IRES -dependent, translation in Drosophila cell-free extracts (A) Cap -dependent translation using cap-FLuc reporter mRNA in the absence or presence of increasing amounts of the indicated proteins...
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Báo cáo y học: "Variations in the transcriptome of Alzheimer''''s disease reveal molecular networks involved in cardiovascular diseases" potx
... Trottier S, Epelbaum J: The proprotein convertase PC2 is involved in the maturation of prosomatostatin to somatostatin-14 but not in the somatostatin deficit in Alzheimer's disease Neuroscience ... located in module Plasminogen activator inhibitor type (PAI-1) proteins inhibit plasminogen activators [44] Therefore, if the level of PAI-1 is high in the AD affected samples, plasminogen activators ... are being inhibited, resulting in decreased levels of mature BDNF Interestingly, the expression levels of TrkB and PAI-1 were elevated in the AD samples However, TrkB is downregulated following...
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A functional genomics approach for elucidation of novel mechanisms involved in GnRH regulation of the gonadotropins
... C domain is the DNA-binding domain, which consists of two zinc-finger-like motifs The D domain is a variable hinge The multifunctional C-terminal (domain E) is the ligand -dependent domain (LBD), ... Hypothalamus-pituitary-adrenal Io Ionomycin IP3 Inositol 1,4,5 triphosphate JNK C-Jun NH2-terminal kinase LB Luria Bertani LH Luteinizing hormone LHR Luteinizing hormone receptor LHβ Luteinizing hormone β-subunit ... 2005) However, in that study, the CaMK inhibitor KN62 did not recapitulate these findings, which suggested that other CaM -dependent signaling mediators also are involved in mediating the GnRH effects...
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Tài liệu Báo cáo khoa học: Molecular cloning, recombinant expression and IgE-binding epitope of x-5 gliadin, a major allergen in wheat-dependent exercise-induced anaphylaxis ppt
... recombinant x-5 gliadin protein that can be used for diagnosis and possibly treatment of WDEIA Results Identification of IgE-binding epitopes in x-5 gliadin The IgE-binding epitopes of x-5 gliadin ... ethanol; lane 4, purified recombinant protein 4434 In this study we identified new linear IgE-binding epitopes in x-5 gliadin and described the gene cloning, expression in E coli, purification, and ... the protein: the C-terminal 178 amino acids, at position 813–1346 in x-5a gene in Fig 2, includes all of the detected IgE-binding epitope sequences After amplification of the DNA encoding this...
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Tài liệu Báo cáo khoa học: Crystal structure of thiamindiphosphate-dependent indolepyruvate decarboxylase from Enterobacter cloacae, an enzyme involved in the biosynthesis of the plant hormone indole-3-acetic acid doc
... in tetramer interface Residues lining the active site cavity are underlined Residues binding ThDP are highlighted with a blue background, and those involved in substrate binding by yellow Amino ... contributing to these interfaces is located in the PYR and middle domains The interface contains 10 hydrogen bonds in IPDC The dimer–dimer interface in IPDC is smaller than the corresponding interface ... completely buried in the cofactor binding cleft Several hydrogen bonds that are responsible for binding and proper orientation of the aminopyrimidine ring, are conserved in all ThDP -dependent enzymes...
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Mechanisms for delayed density-dependent reproductive traits in field voles, Microtus agrestis: the importance of inherited environmental effects doc
... 1996 and maintained high densities in 1997, while the population densities in Kielder were lower in 1996 but increased in 1997 (Fig 1) To increase readability we will in the following refer to ... in reproductive traits seen in the field can be explained by mechanisms whereby the memory of past conditions resides in the individual voles Overwintering voles from two areas that differed in ... als In years with increasing population densities, overwintering animals generally start to breed earlier in the spring and more animals mature in their year of birth than in other years...
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Báo cáo khoa học: Amino acid limitation regulates the expression of genes involved in several specific biological processes through GCN2-dependent and GCN2-independent pathways ppt
... pathway, other signaling mechanisms are involved in the control of gene expression (up and down) in response to amino acid limitation The downstream molecular mechanisms involved in this process could ... described for the amino acid -dependent regulation of several genes, including Chop, Atf3, Cat-1 and insulinlike growth factor binding protein (Igfbp1), making it possible that amino acid availability ... GCN2 dependent when it was not regulated in GCN2) ⁄ ) cells, and GCN2 independent when more than 75% of its induction (or repression) in response to amino acid starvation was maintained in GCN2)...
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Báo cáo khoa học: Eukaryotic class 1 translation termination factor eRF1 ) the NMR structure and dynamics of the middle domain involved in triggering ribosome-dependent peptidyl-tRNA hydrolysis pptx
... (M) domain will be described in detail below The C domain of eRF1 interacts with the C domain of eRF3 [15–18], and the binding of both factors is essential for fast kinetics of the termination ... the protein during the docking to the binding site on the ribosome or other ligands Strikingly, the second most flexible part of the protein (if one does not take into account the N-terminal region ... topology-forming role, allowing the protein backbone to have a specific geometry Several other highly conserved residues may have a functional role by forming an interface for protein–RNA binding FEBS...
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Báo cáo khoa học: Molecular cloning and characterization of methylenedioxy bridge-forming enzymes involved in stylopine biosynthesis in Eschscholzia californica doc
... (S)-cheilanthifoline, not (S)-nandinine [7,30,31] If we assume that (S)-nandinine is produced in E californica, there may be no need for a catalyst from (S)-nandinine to (S)-stylopine However, nandinine could ... (S)-nandinine from (S)-scoulerine in vitro; indeed, CYP719A3 converted (S)-scoulerine to (S)-nandinine with high affinity (Km ¼ 0.54 lm) (Fig 6) Previous reports have shown that (S)-stylopine is ... nandinine based on its total m ⁄ z and fragment ion patterns (data not shown) Nandinine is a scoulerine derivative with the methylenedioxy bridge in ring A (2,3-position), whereas cheilanthifoline...
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Báo cáo Y học: Molecular cloning and characterization of isomultiflorenol synthase, a new triterpene synthase from Luffa cylindrica, involved in biosynthesis of bryonolic acid ppt
... A., Inoue, K., Hiraoka, N., Ikeshiro, Y., Kushiro, T., Shibuya, M & Ebizuka, Y (2001) Cloning and characterization of a cDNA encoding b-amyrin synthase involved in glycyrrhizin and soyasaponin ... Ebizuka, Y (1998) Molecular cloning of cDNA from Panax ginseng The isogene that encodes b-amyrin synthase In Towards Natural Medicine Research in the 21st Century, Excerpta Medica International Congress ... were maintained in a 300-mL Erlenmeyer flask containing 60 mL Linsmaier and Skoog medium [23], supplemented with 100 nM 1-naphthaleneacetic acid in the dark at 25 8C, and subcultured at intervals...
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Báo cáo khoa học: Molecular mechanisms regulating molting in a crustacean Halagowder Devaraj and Ayithan Natarajan ppt
... mitogen-activated protein kinase pathway is involved in a number of cellular changes inducing growth and differentiation in human Schwann cells [7] In cardiac myocytes, protein kinase C generates ... cell cycling in human Schwann cells Receptor tyrosine kinases play a central role in transducing the external signals across cell membranes into the intracellular signaling systems, which in turn ... responses in human Schwann cells [7] Receptor tyrosine kinases transduce the signals via extracellular signal-regulated kinases (ERKs), the serine ⁄ threonine protein kinases belonging to the...
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Báo cáo khóa học: Phospholipase C, protein kinase C, Ca 2+ /calmodulin-dependent protein kinase II, and redox state are involved in epigallocatechin gallate-induced phospholipase D activation in human astroglioma cells ppt
... protein kinase C, Ca2+/calmodulin -dependent protein kinase II, and tyrosine phosphorylation are involved in carbachol-induced phospholipase D activation in Chinese hamster ovary cells expressing ... pathway in many cell types [36], and the inhibitor attenuated EGCG-induced increases in PLD activity, PLC probably regulates PLD through stimulation of this kinase Interestingly, the increase in PLD ... three independent experiments Interestingly, expression of the lipase inactive mutant PLCc1 significantly attenuated EGCG-induced PLD activation (Fig 3D), suggesting that PLC-c1 is involved in this...
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Báo cáo khoa học: Identification of amino acids in antiplasmin involved in its noncovalent ‘lysine-binding-site’-dependent interaction with plasmin pptx
... this protein family The lysine-binding-site-mediated interaction between plasmin and antiplasmin is of great importance in regulating the fibrinolytic process and keeping the plasmin active in the ... C-terminal lysine did not differ Only K436E was less affected by 6-aminohexanoic acid (2.5-fold decrease in reaction rate), again suggesting that this residue is involved in the lysine-binding-site-mediated ... time, as fibrin and antiplasmin compete for active plasmin molecules [7,8] In addition, it is known that the lysine-binding sites in plasminogen are important for binding to other proteins, such...
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Báo cáo khoa học: Functional role of fumarate site Glu59 involved in allosteric regulation and subunit–subunit interaction of human mitochondrial NAD(P)+-dependent malic enzyme pptx
... both being in dimer form in low protein concentrations and being reconstituted into tetramers in high protein concentrations In contrast to the E59Q enzyme, the E59D enzyme has a predominantly ... structure of human m-NAD-ME in complex with fumarate has been determined, the binding network of fumarate in the enzyme has become clear In the fumarate-binding pocket, two arginyl residues, Arg67 and ... and polymers in the range of protein concentrations used (Fig 3GI), indicating that the quaternary stability in the dimer interface is greatly changed by shortening one carbon side chain of Glu59...
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Báo cáo khoa học: Molecular mechanisms in successful peripheral regeneration pot
... [43,44] resulting in polyamines such as putrescine, spermine and spermidine show a strong enhancing effect on neurite outgrowth both in vitro and in vivo [45–47] Mitogen-activated protein (MAP) and ... could indicate insufficient BDNF ⁄ GDNF-mediated support following chronic axotomy These findings are in keeping with 2631 Molecular mechanism in regeneration the strong, 50% reduction in the enduring ... on beta 1-integrin function [115–117], and may be involved in the effects observed in alpha7-deficient mice In keeping with their trimeric and multimodal structure, the various laminins can act...
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Báo cáo Y học: Molecular and biochemical characteristics of a gene encoding an alcohol acyl-transferase involved in the generation of aroma volatile esters during melon ripening pptx
... instructions provided by the manufacturer (Invitrogen) The strain harbouring the correct construction was incubated in selecting liquid medium according to Invitrogen recommendations, until the D600 ... in activity However, in the BEAT sequence, which encodes an enzyme involved in scent production, glycine was substituted by methionine in the conserved triad H-x-x-x-DG In yeast AATs [15], only ... is probably not involved in its expression Search for alcohol acyl-transferase activity of CM-AAT1 and CM-AAT2 recombinant proteins None of the recombinant proteins produced in Escherichia coli...
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Báo cáo hóa học: " Exploring the molecular mechanisms underlying the potentiation of exogenous growth hormone on alcohol-induced fatty liver diseases in mice" ppt
... are indicated in bold/underlined) from the template of a pUC19 plasmid DNA containing GH1 (Xinxiang Medical University, Xinxiang, Henan Province, China) The 677-bp GH1 DNA fragment (including ... and molecular mechanisms of GH on AFLD Viral vectors can induce longerlasting effects than recombinant protein administration and thus avoid the inconvenience of repetitive subcutaneous injections ... least in part, by turning on the hepatic adiponectin-SIRT1-AMPK signaling system and related signaling pathways to ameliorate alcoholinduced impairments in the signaling pathways controlling lipid...
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