Regulation and functional analysis of the tumor suppressor gene product, p53

... synthesize first 29 strand cDNA as well as whole p53 gene The 5’ end of the forward primer starts from the start codon of p53 gene and in case of reverse primer the 5’ end starts just after the ... Regulation of p53 dependent DNA-repair target gene promoters 60 by p53Pro and p53Arg variants VI 3.2.2.1 Expression of Gadd45 promoter in presence of either...

Ngày tải lên: 15/09/2015, 17:10

... to the cytosol Here we confirm and extend earlier studies of these AAA-peroxins and give a further detailed functional analysis of their cassette structure and interaction The interaction of Pex1p ... for the Pex1p Pex6p interaction and their functional role in peroxisome biogenesis Results The contribution of Pex1p and Pex6p to peroxisomal bioge...

Ngày tải lên: 07/03/2014, 16:20

... like the Trp residue of the 183 Conformation, ligand binding and distribution of nematode protein Ag-NPA-1 R Jordanova et al Fig Immunohistological localization of Ag-NPA-1 in adult A galli and ... ligand binding and distribution of nematode protein Ag-NPA-1 Fig Immunogold electron microscopic localization of Ag-NPA-1 in a male A galli worm (A) Sect...

Ngày tải lên: 16/03/2014, 18:20

... pattern of expression of a gene [34–39] To evaluate the relevance of the leader intron in cardosin expression, we deleted it from the 5¢-flanking region of the genes (Fig 8) The deletion of the cardosin ... region of the cardosin B gene (from ) 147 bp to + 238 bp) .The 529 bp of the promoter region of the cardosin A gene that is relevant for gene...

Ngày tải lên: 30/03/2014, 09:20

... Thus, we present the nucleotide sequence, as well as a systematic structural and functional analysis of the 5¢ flanking region of the Bsep gene The 5¢ deletional analysis of the Bsep promoter revealed ... (Fig 6) Interestingly CDCA reduced the activity of the mutant m-126 Luc even below that of the wild-type p-126 Luc Analysis of the binding affinity...

Ngày tải lên: 17/03/2014, 23:20

... activity of DEC1 is also required for the interaction with BMAL1 Binding of DEC1 mutants to a CACGTG E-box To examine the binding ability of DEC1 mutants to the CACGTG E-box in the Dec1 promoter, ... for DEC1 suppressive activity against CLOCK/BMAL1-induced transcription In addition, the N-terminal region of DEC1, including the bHLH domain, interacted with...

Ngày tải lên: 19/02/2014, 16:20

... system is the p53- regulated control of the endosomal compartment of the cell (Fig 6) The results presented here begin to outline the way in which the p53 protein regulates the transcription of selected ... of these events (Fig 6) The p53 network also alters several other physiological processes in the cell that are undoubtedly related to the functions of...

Ngày tải lên: 23/03/2014, 04:21

... detailed knowledge on how Zn2+ ions modulate the conformation and stability of S100A2 will contribute to a better understanding of the regulation of protein function by metal ions, in particular as a ... be suggestive of partial tetramerization (Fig S1) Chemical stability of holo and apo S100A2 proteins The conformational stability of S100A2- wt and...

Ngày tải lên: 30/03/2014, 02:20

... Results Cloning of the 5¢-upstream region of the Pokemon gene To identify the regulatory sequences that control expression of the Pokemon gene, a 2204-bp section of the 5¢-upstream region of the Pokemon ... mechanisms of the Pokemon gene Computer analysis of putative transcription factor-binding sites For a rough understanding of the re...

Ngày tải lên: 30/03/2014, 04:20

... influence of kinesin-1 on the delivery of NCAM to the cell surface in CHO cells 55 Fig 12: Functional analysis of the influence of kinesin-1 on the delivery of NCAMCT to the cell surface ... kinesin-1 and kinesin light chain 13 Fig 4: Analysis of the purification fractions and the concentrate of hNCAM180ID 41 Fig 5: Analysis of the purificatio...

Ngày tải lên: 25/11/2015, 13:27

... of word meal in English and words relating to it (in contrast with Vietnamese equivalents) - Definition of word meal - Field of word meal in English and in Vietnamese equivalents - Cultural and ... with the notion of meal are called field of word meal In our opinion, field of word meal is all the words relating to it mai...

Ngày tải lên: 15/04/2013, 15:11

... S103 G145/D137 Y 112 D2 01/ 193 L75/67 G145/D137 E1 41/ 133 K173 /16 5 R207 /19 9 G77/69 D142 /13 4 G197 /18 9 Fig Superposition of PRTFDC1 with HPRT-ImmGP (Protein Data Bank code: 1BZY) (A) Residues in the ... Welin et al Studies of the human PRTFDC1 B A PRTFDC1 25 HPRT 20 10 00 0.2 800 600 400 0 .1 200 –50 50 10 10 50 10 015 0 200 250 S 200 –50 250 50 12 00 10 0 15 0 [H...

Ngày tải lên: 15/02/2014, 01:20

... 0.1 (10% attacks) We analyze both static and dynamic susceptibility of the Internet to faults and attacks In static analysis, we first reconfirm previous work of Albert et al [5] Based on these results, ... of the Internet’s susceptibility to attacks and faults, and discovered two interesting results; First, the Internet is much more preferential tha...

Ngày tải lên: 18/02/2014, 01:20

... mechanism of both RNA and DNA synthesis [17] In a previous study, we reported the findings of an analysis of the complete sequence of the cryptic plasmid pIT3 isolated from the crenarchaeon S solfataricus ... analyses of the predicted amino acid sequence showed that the C-terminal half of the RepA of the pIT3 plasmid is sequence-similar to the heli...

Ngày tải lên: 18/02/2014, 18:20

... has already established Of two possible dimeric structures of LPL, the head-tohead and the head-to-tail models, the studies of the chimeric proteins of hepatic lipase and LPL and the tandem repeat ... (Fig 4A and B, 5) The lipase activity of the K413A mutant is similar to that of wild type LPL, whereas the esterase activity is reduced to 60% of t...

Ngày tải lên: 21/02/2014, 15:20