neurogenic and functional disorders of the larynx

... II The hood domain is mainly built up by residues from the C-terminus and consists of a two-stranded anti-parallel b-sheet composed of b2 and b9 and an a-helix from the C-terminus (Fig 1A) The ... Lys173 interacts with both O6 and N7 (Fig 1C) The 2¢OH of the ribose is hydrogen bonded to the main chain and the side chain of Asp142 (Fig 1C) Both hydroxyl groups of the ribose are involved in ... Asp142, 2¢ and 3¢OH of the ribose and three water molecules with ˚ coordination distances of approximately 2.4 A The phosphate of the GMP is hydrogen bonded to main chain and side chain atoms of amino...

Ngày tải lên: 15/02/2014, 01:20

... a4-helix to the b4 strand and the b9 strand to the b10 strand at the bottom of the Zn-stem loop in the pRN1 prim–pol structure However, because the Zn-stem loop is a fairly self-standing structure ... 70 and 80 °C between 190 and 260 nm with a step increase of 0.2 nm, and a bandwidth of nm Thermal stability of Rep245 was measured by incubating the protein at 60, 70 and 80 °C for 30 and then ... the two-metal-ion mechanism of both RNA and DNA synthesis [17] In a previous study, we reported the findings of an analysis of the complete sequence of the cryptic plasmid pIT3 isolated from the...

Ngày tải lên: 18/02/2014, 18:20

... the C-terminal domain of apoCII interacts with the interface of the N- and C-terminal domains of LPL and part of the lid surface [32] Because the functional importance of LPL’s C-terminal domain ... illustrates the overall saddle shape of the dimeric structure and the key heparin-binding residues in the N-terminal domain held coordinately by the two subunits on the top of the hollow (Fig 1A) The ... not the lipase activity and that the normal affinity of these mutants for heparin (Fig 3, Table 1) implies conservation of the overall structure of the WW and WWW mutants – indicate that these...

Ngày tải lên: 21/02/2014, 15:20

... to the cytosol Here we confirm and extend earlier studies of these AAA-peroxins and give a further detailed functional analysis of their cassette structure and interaction The interaction of Pex1p ... of Pex1p and Pex6p A B Fig Two-hybrid interaction of Pex1p and Pex6p harboring point mutations of the WalkerA and B motifs of their ATP-binding sites A schematic representation of the Pex1p and ... the binding sites of these two AAA-proteins to their different protein regions and elucidated the importance of ATP-binding to the two AAA-cassettes of Pex1p and Pex6p for their interaction The...

Ngày tải lên: 07/03/2014, 16:20

... of the medium and K2 the orientation factor, determined by the mutual spatial orientation of the transition dipole moments of the donor and acceptor As no data on the spatial orientation of the ... in cells of the inner hypodermis and the epithelium of the oviduct as well as extracellularly in the pseudocoelomic cavity of the worms Discussion A bundle of four a-helices constitutes the secondary ... determined pI of 6.1 and the theoretically deduced pI value of 6.22 was found A theoretical prediction of the secondary structural organization of Ag-NPA-1 performed on the basis of the amino acid...

Ngày tải lên: 16/03/2014, 18:20

... significantly in the presence or absence of the b1B subunit, whereas, under the same conditions, the rate of inactivation was increased in the presence of the b1 subunit The effects of b1B were further assessed ... in Fig 4A–D, the rates of activation and inactivation of the sodium current via 4768 N Qin et al (Eur J Biochem 270) Ó FEBS 2003 Fig The effect of b1B and b1 subunits on the function and expression ... part of intron adjacent to exon (Fig 1C and Table 1) As the site of divergence between the b1 and b1B subunit cDNAs was located precisely at the exon 3/intron boundary of the SCN1B gene, the human...

Ngày tải lên: 16/03/2014, 23:20

... deciphering the structural and functional organization of the synapse We have assembled leaders in the field of synapse biology to describe and distill the wonders and mysteries of the synapse ... must occur within the tiny space of the synapse, the precise molecular organization of proteins, lipids, and membranes at the synapse is paramount Given the central role of the synapse in neuronal ... STRUCTURAL AND FUNCTIONAL ORGANIZATION OF THE SYNAPSE STRUCTURAL AND FUNCTIONAL ORGANIZATION OF THE SYNAPSE Edited by Johannes W Hell University of Iowa Iowa City, IA, USA and Michael D Ehlers...

Ngày tải lên: 06/07/2014, 15:30

... subcloned into the plasmid pGEM-3Z to generate the clone pGS217 and used for analysis and sequencing 2.2 Fusions of the 5’region of GS1a to the GUS reporter gene The 981 bp sequence upstream of the translation ... [4,16,18], and none of them correspond to nitrogen metabolism We have studied the promoter activity of the 5’-untranslated region using fusions with the reporter gene uidA and the presence of DNA-protein ... tissues of developing seedlings and the level of the transcript was affected by developmental and light conditions [9] Here, we present the DNA sequence of a partial genomic clone containing exons of...

Ngày tải lên: 08/08/2014, 14:20

... synthesize first 29 strand cDNA as well as whole p53 gene The 5’ end of the forward primer starts from the start codon of p53 gene and in case of reverse primer the 5’ end starts just after the ... role in the synthesis of DNA and is responsible for the reduction of ribonucleotides to their corresponding deoxiribonucleotides, providing a balanced supply of precursors for DNA synthesis and repair ... without disturbing the pellet 400μl of 95% ethanol was then added to the clear lysate, mixed and the mixture was transferred to the spin column The supernatant was then passed through the cartridge...

Ngày tải lên: 15/09/2015, 17:10

... originate in the ‘transformation zone’ which is located at the lower end of the cervix where the columnar cells of the endocervix form a junction with the stratified squamous epithelium of the vagina ... spectrum of abnormalities of the cervical epithelium (Richart and Barron, 1969) These lesions are classified as cervical intraepithelial neoplasia (CIN) grades 1, and The severity of the lesion ... between the L1 and E6 ORFs (Figure 3) Figure 3: Organization of the genome of HPV 16 Most of the ORFs overlap and the early genes are all transcribed from a single promoter, P97, upstream of the...

Ngày tải lên: 17/09/2015, 17:18

... rings and the catheter was inserted such that the bevelled end headed towards the lungs The cathether and the trachea were tied together so as to secure the cathether in position The right common ... blood inflow to the lacunar spaces increases Relaxation of the smooth muscle of the trabeculae then dilates the lacunae, allowing for the expansion of the erectile tissue against the tunica albuginea ... the effects of H2S may be twofold; being involved in 1) the relaxation of the corporal smooth muscle; and 2) the inhibition of the penile basal tone The nature and site of H2S effects (molecular/cellular/neurovascular)...

Ngày tải lên: 12/10/2015, 17:33

Ngày tải lên: 13/03/2016, 13:26

... glutamate and aspartate residues confined to the portion of the molecule involving a-helix A, the nearby loop, the first and last turns of helices E and F, and the C-terminus helices I and J In ... b-strand, F, which protrudes from the core of the monomer and runs anti-parallel to the same strand of a second monomer, allowing for the formation of the dimer The surface of interaction between monomers ... as that of other members of this family Finally, when crystallized in the presence of Cu(II), the protein shows the presence of the ion coordinated in a small cavity of the surface at the polar...

Ngày tải lên: 06/03/2014, 00:21

... that the nonconserved PilW protein is implicated in the assembly and stability of PilQ multimers at the inner membrane and transport of these subassemblies to the outer membrane The presence of the ... pilQ led to the absence of PilW and PilA4 in the inner membrane In addition, pilW mutation resulted in the absence of PilQ and PilA4 in the outer membrane The abilities of PilW, PilQ and PilA4 ... machineries of mesophilic bacteria, together with the effect of a pilW mutation on the biogenesis and ⁄ or stability of PilA4 and PilQ in the outer membrane, indicate that PilW is a special feature of the...

Ngày tải lên: 07/03/2014, 12:20

... interaction of the membrane-bound AAA complex and the importomer Therefore, the protein complexes isolated with protein A fusions of Pex1p, Pex6p and Pex15p were tested for the presence of peroxins of the ... contaminate the eluates (Fig 3A and data not shown), thereby confirming the specifity of the isolation Taken together, these data indicate a close association of the membrane-bound AAA complex and the ... Moreover, these data indicate that at least one of the contact sites of the AAA complex and the importomer is made by Pex15p To investigate the influence of individual peroxins of the importomer on the...

Ngày tải lên: 07/03/2014, 12:20

... between the fully active alpha isoform and other variants, we conducted a series of cotransfection experiments and compared ligand-induced accumulation of cAMP (Fig 3) Although the level of cAMP ... assembled by PCR of these two fragments with primers E3 and E11 Two different constructs were produced for CRFR1h isoform The first contained exons 1–4 and a fragment of the cryptic exon up to the translation ... (5¢-GGTAGTGCACCTTGCTTTTTTTCTCTCCCCA3¢) and attached to another fragment of CRF receptor in the final PCR by primers E3 and E11 To attach V5 epitope to the CRFR1a, g, h2 and e2 isoforms we amplified the corresponding...

Ngày tải lên: 07/03/2014, 15:20

... VBARP Also, the use of unsynchronized cells in these analyses further confirmed that the cytoplasmic distribution of VBARP is independent of the cycling stage of the cells Identification of biological ... the amount of protein used in this assay indicating that an equal amount of protein was used in all our samples and that the lack of interaction between VBARP and Nef is not due to the lack of ... further confirming the specificity of this interaction Based on these results we were able to demonstrate the formation of a complex between Vpr and VBARP Characterization of VBARP variants The...

Ngày tải lên: 07/03/2014, 21:20

... on the one hand and lipid membranes with little incorporated protein on the other [48] The success of this method thus probably depends on the relative affinities of interaction of the protein and ... was present on the surface of transfected cells, and had the expected molecular mass To test whether the presence of the myc-his tag at the C-terminus of CCR5 would interfere with the protein’s ... glycosaminoglycans [37] As the myc-his tag of the protein was present at the C-terminus of the receptor which is located on the inside of the cell, we then determined if signal transduction by the protein was...

Ngày tải lên: 08/03/2014, 09:20

... eliminate the possibility of the functional roles of the )643T>C and )1219G>A SNPs as part of the extended APOH promoter transcriptional machinery To further substantiate the functional relevance of the ... conjunction with the luciferase gene in pGL3basic vector The nucleotides are numbered from the translation start site The effect of the wild-type and mutants was measured as the mean of the firefly luciferase ... tested 12 of the 14 sequence variants located within the 1418 bp of the 5¢ flanking region of APOH for allele-specific regulatory effects on the expression of the dual luciferase reporter gene and by...

Ngày tải lên: 15/03/2014, 10:20

... energies of the original resiDG i;j due i in the native protein with the rest of the residues, self DDG k mutị are the changes of the self energies of the residues k that change either their ionization ... on the reaction Analysis of the effect of the mutations on protein stability revealed three distinctive mechanisms of relaxation, and in the case of S36A, no relaxation at all The rst type of ... distal point of the rest of the protein Such a dipole will have weak interactions with the rest of the protein Thus, if turned off (by removal of Arg and protonation of Asp185), the protein energy...

Ngày tải lên: 16/03/2014, 10:20