Isolation and characterization of aurora a kinase interacting protein (AKIP), a novel negative regulator for aurora a kinase

... Hypothesis of Possible Anti-Tumour Role of AKIP-mediated UbIndependent Degradation of Aurora- A, 234 ix Abbrevations aa A Box AD ADH AIK1 AKIP ALLM ALLN AML Amp APC/C ATP AURKA AZ AZI amino acid Aurora ... yeast and successfully isolated a novel negative regulator of Aurora- A kinase, named as AKIP (Aurora- A Kinase Interacting Protein) AKIP is an ubiquitous...

Ngày tải lên: 14/09/2015, 22:15

... pair: 5¢-AATACCAAAGAAGCCTACAATG-3¢ and 5¢-GTACGAAGTGGAGGTATGTCATC-3¢ b Primer pair: 5¢-GCCATTT TAGGTGCTATTCTGG-3¢ and 5¢-TATTTTCTTTATCTGAGTTTA-3¢ c Primer pair: 5¢-CATCCATAGCAGATAACAGTC-3¢ and ... Ovarya Peripheral blood leukocytea Prostatea Small intestinea Spleena Testisa Thymusa Bone marrowa Fetal livera Lymph nodea Tonsila Breastb Lungb Milk cellsa Bovine Mammary glandc Mammary glan...

Ngày tải lên: 24/03/2014, 04:21

... highest identity with the BAS1 protein of Brassica, spinach, barley and A thaliana, PR1 of Phaseolus and MHF of A thaliana These proteins belong to the 2Cys-Prx subfamily All plant 2Cys-Prx proteins, ... very weak when Trx m from Chlamydomonas was used (data not shown), probably because of the weaker af®nity of Arabidopsis NTR for Trx m [21] Therefore, the ability of T...

Ngày tải lên: 08/03/2014, 16:20

... Nagasawa T, Ohkishi H, Kawakami B, Yamano H, Hosono H, Tani Y & Yamada H (1982) 3-chloro-d-alanine chloride-lyase (deaminating) of Pseudomonas putida CR 1–1 Purification and characterization of ... molecular mass of 41.7 kDa and a pI of 6.34 The YedO protein from E coli and the d-CDes from Arabidopsis showed an overall identity of 36% and a similarity of 50% T...

Ngày tải lên: 16/03/2014, 18:20

... Temperature-dependent ricin A chain mutants Fig Stability of mutant ricin A chains The kinetics of protein degradation of (A) Kar2SP-RTAE177D and (B) Kar2SP-RTAF108L ⁄ L151P at all temperatures, or a cytosolic ... 5¢-ATATTCCCCAAACAATACCC-3¢ and the antisense primer CP133 5¢-TTAAAACTGTGACGATGGT GGA-3¢ with the TAA termination anticodon shown in bold Amplification react...

Ngày tải lên: 23/03/2014, 07:20

... strain to those of the other simian rotavirus strains, SA11 and RRV Materials and methods Rotavirus isolation The YK-1 strain of simian rotavirus was isolated from the diarrheal stool of a 2-year-old ... was based on a Jennerianapproach prompted by studies indicating that animal and human rotaviruses share a common group antigen and that experimental animals immunize...

Ngày tải lên: 20/06/2014, 01:20

... important for ATP binding, has no kinase activity [13] To examine whether purified GST-AtHaspin has kinase activity, an in vitro kinase assay was performed using purified GST-AtHaspin and GST-AtHaspin ... Dlk/ZIP kinase orthologues, and thus, AtHaspin has an additional role as a H3 Thr11 kinase in A thaliana Phosphorylation of histone H3 at Thr3 and Thr11 Mitotic...

Ngày tải lên: 11/08/2014, 11:22

... characterization of a virus (CvV-BW1) that infects symbiotic algae of Paramecium bursaria in Lake Biwa, Japan Virology Journal 2010 7:222 Submit your next manuscript to BioMed Central and take ... Japan, China, and Australia as well as the United States bursaria Chlorella [PBCV], and Acanthocystis turfacea Chlorella virus [ATCV]) and strange names b...

Ngày tải lên: 12/08/2014, 01:21

... Bezanilla JC, Toth TE, Meng XJ: Identification of genotype hepatitis E virus (HEV) in serum and faecal samples from pigs in Thailand and Mexico, where genotype and HEV strains are prevalent in ... genotype HEV strains with complete genome available in GenBank (including both swine and human original isolates) were used as references in the analysis; their Gen...

Ngày tải lên: 12/08/2014, 04:21

... -HGA ( M) Figure and antiviral comparison of α-HGA and some structurally related compounds Biological Biological and antiviral comparison of α-HGA and some structurally related compounds Chemical ... characterization of G-NH2 metabolite obtained after dialysis against FCS or PS Antiviral activity of G-NH2 and characterization of G-NH2 metabolite obtained after dialysis...

Ngày tải lên: 12/08/2014, 23:20

... natural activated sludge An extracellular polymer was extracted from an activated sludge suspension and was characterized because an extracellular polymer closely related to flocculation of activated ... drawn from the results of the study An extracellular polymer closely related to flocculation of activated sludge was obtained from th...

Ngày tải lên: 05/09/2013, 09:08

... Purification of four pulchellin isoforms from A pulchellus seeds Using a combination of affinity, ion exchange and chromatofocusing chromatography, four pulchellin isoforms were isolated from A pulchellus ... II, III and IV and refers to each pulchellin Characterization of four pulchellin isoforms isoform (P I, P II, P II and P IV) The A-chain of...

Ngày tải lên: 18/02/2014, 16:20

... aminoacids at the N-terminus of PHI (sequence: SKVYLALQDND) were compared with the sequences of the so called ‘intermediate components’ from two other PHs The N-terminal sequence of PHI from A radioresistens ... one PHR and one PHO (abc) protomer The hypothesis of a direct PHI phenol interaction is quite unlikely, because of the fact that the addition of ph...

Ngày tải lên: 21/02/2014, 00:20

... describes the relationship between the heme core description and the spectroscopic and redox properties of each identified heme from the NrfHA complex Conclusions D desulfuricans ATCC 27774 ccNiR ... this communication, we report for the first time the isolation and biochemical characterization of D desulfuricans ATCC 27774 ccNiR subunits The st...

Ngày tải lên: 21/02/2014, 00:20

... lg of protein) were separated by SDS/PAGE using the standard Laemmli system [30] with 13% and 4% (w/w) acrylamide in the separating and stacking gels, respectively In order to estimate the ratio ... they are acidic and presumably located in the cytoplasm like UspA Members of this family share a strikingly similar hydropathy profile (data not shown), and UP12 shares...

Ngày tải lên: 22/02/2014, 07:20