Differentiation of bone marrow derived mesenchymal stem cells (BM MSCs) using engineered nanofiber substrates

... Types of cells used in bone tissue engineering for osteogenic differentiation 59 2.3.1 Potential of mesenchymal stem cells (MSCs) for bone healing 60 2.3.2 Potential of bone marrow derived mesenchymal ... Casey K Chan Effects of mechanical stimulation in osteogenic and chondrogenic differentiation of bone marrow- derived mesenchymal stem cells on...

Ngày tải lên: 11/09/2015, 09:57

... Dou KF, Yang GS, Willerson JT, Geng YJ: Combined therapy with simvastatin and bone marrow-derived mesenchymal stem cells increases benefits in infarcted swine hearts Arterioscler Thromb Vasc Biol ... neovascularization in murine hindlimb ischemia by combined therapy with simvastatin and bone marrow-derived mesenchymal stem cells transplan...

Ngày tải lên: 10/08/2014, 05:21

... of stem cells is a promising method for cartilage repair, in this chapter I confirmed that engraftment of the MSCs in injured cartilage is an active migration and homing process and injured cartilage ... microfracture (265, 266), or introducing new cell sources like debrided waste chondrocytes, Bone Marrow derived Mesenchymal Stem Cells (BM MSCs), or any combinati...

Ngày tải lên: 10/09/2015, 09:01

...   1. 5 Types of stem cells 14   1. 5 .1 Embryonic stem cells (ESCs) 15   1. 5.2 Induced pluripotent stem (iPS) cells 15   1. 5.3 Adult stem cells 16   1. 5.4 Heterologous stem cells 17   1. 6 MSCs in ... 1. 10.3 Iron Oxide Particles 24   1. 11 Cellular MRI 24   1. 11. 1 Cellular Imaging with Iron Oxide Particles 25   1. 11. 2 Mechanisms of Cellular Uptake 28   1....

Ngày tải lên: 10/09/2015, 09:10

... determined using 0.3% oil red O stain for 15 minutes at room temperature to stain intracellular lipids, and counterstained with hematoxylin Osteogenic differentiation was evaluated using Alizarin ... staining of the harvested tissues (197) 55   Table 3-1 Histological grading scale for cartilage repair (Adapted from (51)) Category Points Cell morphology Hyaline cartilage Mostly hyaline...

Ngày tải lên: 10/09/2015, 09:10

... Oil red O staining demonstrated that unlabeled and labeled cells exhibited no difference in developing fat vacuoles (Figure 3. 9) 64     Figure 3- 9 Oil red O staining Oil red O staining of the ... (F) 3. 4.6.2 Osteogenic differentiation Alizarin red staining showed that MSCs labeled with SPIO did not affect osteogenic differentiation capacity of cells (figure 3. 10)   Figure 3- 10 Ali...

Ngày tải lên: 10/09/2015, 09:11

... labeled cells in the knee joint) MSCs were distributed in different places of the knee (figure 3. 14) The labeled cells migrated to different locations over time including cartilage defect, injection ... MSCs in the mini-pig knee joint The labeled cells migrated to the para-patellar fat tissue (A) and surgical scar in the skin (B) 3 .4. 8 Postmortem analysis Histological grading sco...

Ngày tải lên: 10/09/2015, 09:11

Ngày tải lên: 10/09/2015, 09:11

... confirming the report of Jing et al (2 06, 207) In addition, our results showed that we could detect labeled cells in vivo up to weeks, thus the SPIO labeling method of the cells can be a promising ... labeling concentration of MSCs can reduce the adverse effects of the iron particles Our results showed, after optimized labeling, that visualization of injected MSCs is indeed feasible in...

Ngày tải lên: 10/09/2015, 09:11

... articular cartilage provides only a limited capacity of self -repair Cell based therapy is one promising approach in the treatment of damaged cartilage Bone marrow (BM) derived mesenchymal stem cell (MSC) ... Leukemia inhibitory factor Neurotrophin Chemokine (C-X-C motif) ligand 10 Colony stimulating factor (gran-macrophage) Colony stimulating factor (granulocyte) I...

Ngày tải lên: 10/09/2015, 09:11

... Sphingosine-1-phosphate promotes the differentiation of human umbilical cord mesenchymal stem cells into cardiomyocytes under the designated culturing conditions Journal of Biomedical Science 2011 ... have successfully produced both neonatal cardiac myocyte and cardiomyocytes sheets from differentiated human umbilical cord mesenchymal stem cells...

Ngày tải lên: 10/08/2014, 05:21

... this article as: Yang et al.: High efficient isolation and systematic identification of human adipose-derived mesenchymal stem cells Journal of Biomedical Science 2011 18:59 Submit your next manuscript ... differentiation of cryopreserved human adipose-derived stem cells Cryobiology 2008, 57:18-24 Chen MY, Lie PC, Li ZL, Wei X: Endothelial differentiation of...

Ngày tải lên: 10/08/2014, 10:20

... Transplantation of canine umbilical cord blood-derived mesenchymal stem cells in experimentally induced spinal cord injured dogs J Vet Sci 2007, 8, 275-282 22 Lu J, Descamps M, Dejou J, Koubi G, Hardouin ... implanted in an appropriate matrix ectopically or orthotopically [3,14] Implants with autologous bone marrow MSCs contain more new bones within and around implan...

Ngày tải lên: 07/08/2014, 23:22

... recognized by the SH3 antibody, and express prolyl-4-hydroxylase Prolyl-4-hydroxylase was shown to be expressed by synoviocytes [18,24] but to be absent on BM-MSC using FACS analysis [18] In ... differentially expressed in bone marrow-derived or synovium-derived cells Genes whose mRNA expression is differentially expressed in bone marrow-derived or synovium-derived cells Gene Up...

Ngày tải lên: 09/08/2014, 07:20

... HIV-1 and recombinant gp120 affect the survival and differentiation of human vessel wall-derived mesenchymal stem cells Retrovirology 2011 8:40 Submit your next manuscript to BioMed Central and ... muscle and endothelial cells To study the effects of HIV-1 on the differentiation of these cells, the interaction of HIV-1 and recombinant gp120...

Ngày tải lên: 13/08/2014, 01:20