... and bone marrow- derived MSCs Bone marrow- derived MSCs have been identified as a potential new therapeutic option to induce therapeutic angiogenesis The main advantage of using bone marrow- derived ... of bone marrow- derived MSCs Flow cytometric analyses of bone marrow- derived MSCs Cells were uniformly negative for CD34, CD45, and positive for CD44, and CD29 Figure Effect of simvastatin and bone ... on the cell viability of bone marrowderived MSCs in vitro To examine whether simvastatin has anti-apoptotic effect on bone marrow- derived MSCs under hypoxia stress, cells viability was detected...
... in bone tissue engineering for osteogenic differentiation 59 2.3.1 Potential of mesenchymalstemcells (MSCs) for bone healing 60 2.3.2 Potential of bonemarrowderivedmesenchymalstemcells ... Potential of adipose -derived stemcells 63 2.3.4 MSCs derived from other sources 64 2.4 Cell-material constructs as bone grafts 66 2.4.1 Mesenchymalstemcells (MSCs)-material ... BM-MSCs: BoneMarrowderivedMesenchymalStemCells BCA: Bicinchoninic Acid BET: Brunauer, Emmett and Teller analysis α-TCP: Alpha-Tricalcium Phosphate β-TCP: Beta-Tricalcium Phosphate BM: Bone marrow...
... common pathways mediating differentiation of bone marrowand adipose tissue -derived human mesenchymalstemcells into three mesenchymal lineages StemCells 2007 Mar;25(3):750-60 PubMed PMID: 17095706 ... the stemcells and/or attract the endogenous or exogenous stemcells (endogenous from the bonemarrow and exogenous by multiple intra-articular injection of the expanded autologous stem cells) ... Kanawa M, Nikawa H, et al Comprehensive analysis of chemotactic factors for bonemarrowmesenchymalstemcellsStemCells Dev 2007 Feb;16(1):119-29 PubMed PMID: 17348810 Epub 2007/03/14 eng 158...
... than 98% of cells were negative for hematopoietic markers (CD14, CD31, CD34, and CD45), and endothelial marker (CD31) (figure 3.3) 58 Figure 3-3 Flow cytometry analysis of the stemcells surface ... (Table 3.1) The iron-labeled cells within the repaired defect were visualized by Prussian blue staining (197) Localization, fate and possible interaction of the iron-labeled cells with the surrounding ... Characterization of MSCs Cells cultured from aspirated BM had characteristic of fibroblastic spindleshape morphology and flow cytometry confirmed that more than 96% of the cells were positive for...
... the MSCs did not affect the proliferation rate of the cells over time 3.4.6 Differentiation potential of labeled MSC MSCs are multipotent stem cells, which can differentiate to different lineage ... pig’s knee explants with blank and scaffold only defect (A), 100 cells simulation (B), 1000 cells simulation (C), and 100,000 cells simulation (D) Left panel of each image is 3D-FSE sequence ... differentiation capacity of cells (figure 3.10) Figure 3-10 Alizarin Red staining Alizarin Red staining of the MSCs was used to visualize the calcium deposition of the cells after 21 days osteogenic...
... site and arrows demonstrate the labeled stem cells) 72 Figure 3-16 MRI of the distribution of the labeled MSCs in the mini-pig knee joint The labeled cells migrated to the para-patellar fat ... (Arrowheads show the distribution of the labeled cells in the knee joint) MSCs were distributed in different places of the knee (figure 3.14) The labeled cells migrated to different locations over ... making the defect (before injection), immediately after injection, two and six weeks after the cells injection Upper panel (right knee) show the knee injected with HA plus labeled MSCs, however...
... chondrogenic differentiation Farrell et al (204) showed that SPIO labeled cells negatively affected the osteogenic differentiation of the cells in vivo In contrast, our study showed that iron labeling did ... (189) This difference could be the result of lower loading of the cells (5 pg iron per cell) by Heymer et al., which is similar to our cells iron content labeled with lower labeling concentration ... MSCs is indeed feasible in vivo Cells migration and localization into different site of the inflammation was observed in the knee joint over time A majority of the cells moved to the surgery sites...
... Bonemarrow (BM) derivedmesenchymalstem cell (MSC) is a good candidate because of their multipotent nature The use of MSCs for cartilage repair relies on the homing and engraftment of the cells ... transforming growth factor beta-3 (TGFβ3), bone morphogenetic protein (BMP4), vitronectin (VTN) Conclusion: As I showed in the previous chapter, injection of stemcells in the knee is a promising method ... biochemical factors essential in mimicking physiological conditions for cells after administration Key words: Mesenchymalstem cell (MSC), cell migration, microfluidic device, cartilage, tissue...
... expressed in bone marrow- derived or synovium -derived cells Genes whose mRNA expression is differentially expressed in bone marrow- derived or synovium -derived cells Gene Upregulated in bone marrow- derived ... whereas it was negative Figure derived adherent cells Immunosuppressive properties of bone marrow- derived and synoviumderived adherent cells (a) Proliferative activity of T cells in a mixed lymphocyte ... out of six different samples of bonemarrowmesenchymalstemcells (three normal, two osteoarthritis and one rheumatoid arthritis) and synovium mesenchymalstemcells (two rheumatoid arthritis,...
... may provide sufficient seeding cells for tissue engineering applications Bonemarrow stromal cells (MSCs) are multipotent stemcells originating from the bonemarrow stroma, and represent a particularly ... osteogenic and endothelial cells Methods Cell Culture and Induction Bonemarrowcells were obtained from male BALB/c mice (6–8 weeks of age) Low-density bonemarrow mononuclear cells (MNCs) were isolated ... [5,6] In contrast, bone marrow- derived endothelial progenitor cells (EPCs) possess high potential for neovascularization and reendothelialization [7] EPCs isolated from bonemarrow or peripheral...
... bone defects J Bone Joint Surg Am 1998, 80, 985-996 Chang YJ, Shih DT, Tseng CP, Hsieh TB, Lee DC, Hwang SM Disparate mesenchyme-lineage tendencies in mesenchymalstemcells from human bonemarrow ... 12 Kadiyala S, Jaiswal N, Bruder SP Culture-expanded, bone marrow- derivedmesenchymalstemcells can regenerate a critical-sized segmental bone defect Tissue Eng 1997, 3, 173-185 13 Kadiyala S, ... umbilical cord blood StemCells 2006, 24, 679-685 Chen L, Tredget EE, Wu PYG, Wu Y Paracrine factors of mesenchymalstemcells recruit macrophages and endothelial lineage cells and enhance wound...
... Currently, several candidates of stem cells, including embryonic stem cell, neuron stem cells, bone marrow- derivedmesenchymalstem cell, and peripheral blood -derived stemcells have been frequently ... adipose -derived mesenchymalstemcells (ADMSCs) for the treatment of stroke has seldom been discussed [19] Compared with embryonic stems cells and bone marrow- derivedmesenchymalstem cells, ADMSCs ... transplanted cells into the host tissue [3] Interestingly, while stem cell therapy, including bone marrow- derivedmesenchymalstemcells [15-17], embryonic stemcells [14] and endothelial progenitor cells...
... integration of the transplanted cells and the host tissue As compared with embryonic stems cells and bone marrow- derivedmesenchymalstem cells, adiposederived mesenchymalstemcells (ADMSCs) have the ... Previous study has also demonstrated a therapeutic superiority of ADMSCs over bone marrowderived mesenchymalstemcells in an animal model of liver injury [14] It is, therefore, conceivable that ... (ADMSC-treated) showing few troponin I-positive myogenic-like cells (pink arrows) and undifferentiated adipose -derived mesenchymalstemcells (ADMSCs) (yellow arrows) in LV myocardium Merged image...
... reported [23] Besides, although bone marrowderived MSC is the major source of stemcells in these studies [22,24-27], the therapeutic role of adiposederived mesenchymalstemcells (ADMSCs) in acute IR ... endotoxin-induced systemic response by bone marrow- derivedmesenchymalstemcells in mice Am J Physiol Lung Cell Mol Physiol 2007, 293:L131-141 23 Salgar S, Manning E, Ruiz P, Pham S: MesenchymalStem Cell ... 2010, 17:92 21 Rojas M, Xu J, Woods CR, Mora AL, Spears W, Roman J, Brigham KL: Bone marrow- derivedmesenchymalstemcells in repair of the injured lung Am J Respir Cell Mol Biol 2005, 33:145-152...
... [1,8] Adult stemcells are known to have a limited differentiation potential while embryonic stemcells are totipotent Multipotent stemcells were first isolated from adult bonemarrow [17] The ... anti-canine CD45, mouse anti-canine 49b, mouse anti-canine CD 51/61, mouse anti-canine CD62p, mouse anti-canine CD73, mouse anti-canine CD90, mouse anti-canine CD105, mouse anti-canine CD133, mouse anti-canine ... CD34(+) bonemarrowcells Blood 2000, 95, 581-585 Bieback K, Kern S, Klüter H, Eichler H Critical parameters for the isolation of mesenchymalstemcells from umbilical cord blood StemCells 2004,...
... annulus cells adiposederived mesenchymalstem of AD-MSCfor 3D co cultured alone Increase in separate culture cells (AD-MSC) and annulus cells comderived mesenchymalstemcells (AD-MSC) and annulus cells ... sand rat adipose -derived mesenchymal extracellular 3D factor in the Immunohistochemical documentation ofstem cellsgrowthculture beta sand rat adipose -derived mesenchymalstemcells in 3D culture ... nucleus pulposus cells could secret soluble factors to direct stemcells towards the nucleus pulposus phenotype [41] Previous work on interactions of adult mesenchymalstemcells and disc cells by Le...