mouse bone marrow derived mesenchymal stem cells

... and bone marrow- derived MSCs Bone marrow- derived MSCs have been identified as a potential new therapeutic option to induce therapeutic angiogenesis The main advantage of using bone marrow- derived ... of bone marrow- derived MSCs Flow cytometric analyses of bone marrow- derived MSCs Cells were uniformly negative for CD34, CD45, and positive for CD44, and CD29 Figure Effect of simvastatin and bone ... on the cell viability of bone marrowderived MSCs in vitro To examine whether simvastatin has anti-apoptotic effect on bone marrow- derived MSCs under hypoxia stress, cells viability was detected...

Ngày tải lên: 10/08/2014, 05:21

... in bone tissue engineering for osteogenic differentiation 59 2.3.1 Potential of mesenchymal stem cells (MSCs) for bone healing 60 2.3.2 Potential of bone marrow derived mesenchymal stem cells ... Potential of adipose -derived stem cells 63 2.3.4 MSCs derived from other sources 64 2.4 Cell-material constructs as bone grafts 66 2.4.1 Mesenchymal stem cells (MSCs)-material ... BM-MSCs: Bone Marrow derived Mesenchymal Stem Cells BCA: Bicinchoninic Acid BET: Brunauer, Emmett and Teller analysis α-TCP: Alpha-Tricalcium Phosphate β-TCP: Beta-Tricalcium Phosphate BM: Bone marrow...

Ngày tải lên: 11/09/2015, 09:57

... common pathways mediating differentiation of bone marrowand adipose tissue -derived human mesenchymal stem cells into three mesenchymal lineages Stem Cells 2007 Mar;25(3):750-60 PubMed PMID: 17095706 ... the stem cells and/or attract the endogenous or exogenous stem cells (endogenous from the bone marrow and exogenous by multiple intra-articular injection of the expanded autologous stem cells) ... Kanawa M, Nikawa H, et al Comprehensive analysis of chemotactic factors for bone marrow mesenchymal stem cells Stem Cells Dev 2007 Feb;16(1):119-29 PubMed PMID: 17348810 Epub 2007/03/14 eng 158...

Ngày tải lên: 10/09/2015, 09:01

... cartilage repair such as bone marrow derived stem cells (BM MSCs), adipose derived stem cells (ADSCs), periostal cells, skeletal muscle and synovial fibroblasts derived cells (63-68), 12   which ... cell components Mesenchymal stem cells Bone marrow, peripheral blood Bone, Cartilage, tendon, adipose tissue, muscle, marrow stroma, neural cells Neural stem cells Ependymal cells, astrocytes ... cells Pancreatic stem cells Intraislet, nestin-positive cells, oval cells, duct cells Beta cells Skletal-muscle stem cells or satellite cells Muscle fibers Skeletal muscle fibers Stem cells of the...

Ngày tải lên: 10/09/2015, 09:10

... than 98% of cells were negative for hematopoietic markers (CD14, CD31, CD34, and CD45), and endothelial marker (CD31) (figure 3.3) 58     Figure 3-3 Flow cytometry analysis of the stem cells surface ... (Table 3.1) The iron-labeled cells within the repaired defect were visualized by Prussian blue staining (197) Localization, fate and possible interaction of the iron-labeled cells with the surrounding ... Characterization of MSCs Cells cultured from aspirated BM had characteristic of fibroblastic spindleshape morphology and flow cytometry confirmed that more than 96% of the cells were positive for...

Ngày tải lên: 10/09/2015, 09:10

... the MSCs did not affect the proliferation rate of the cells over time 3.4.6 Differentiation potential of labeled MSC MSCs are multipotent stem cells, which can differentiate to different lineage ... pig’s knee explants with blank and scaffold only defect (A), 100 cells simulation (B), 1000 cells simulation (C), and 100,000 cells simulation (D) Left panel of each image is 3D-FSE sequence ... differentiation capacity of cells (figure 3.10)   Figure 3-10 Alizarin Red staining Alizarin Red staining of the MSCs was used to visualize the calcium deposition of the cells after 21 days osteogenic...

Ngày tải lên: 10/09/2015, 09:11

... site and arrows demonstrate the labeled stem cells) 72     Figure 3-16 MRI of the distribution of the labeled MSCs in the mini-pig knee joint The labeled cells migrated to the para-patellar fat ... (Arrowheads show the distribution of the labeled cells in the knee joint) MSCs were distributed in different places of the knee (figure 3.14) The labeled cells migrated to different locations over ... making the defect (before injection), immediately after injection, two and six weeks after the cells injection Upper panel (right knee) show the knee injected with HA plus labeled MSCs, however...

Ngày tải lên: 10/09/2015, 09:11

Ngày tải lên: 10/09/2015, 09:11

... chondrogenic differentiation Farrell et al (204) showed that SPIO labeled cells negatively affected the osteogenic differentiation of the cells in vivo In contrast, our study showed that iron labeling did ... (189) This difference could be the result of lower loading of the cells (5 pg iron per cell) by Heymer et al., which is similar to our cells iron content labeled with lower labeling concentration ... MSCs is indeed feasible in vivo Cells migration and localization into different site of the inflammation was observed in the knee joint over time A majority of the cells moved to the surgery sites...

Ngày tải lên: 10/09/2015, 09:11

... Bone marrow (BM) derived mesenchymal stem cell (MSC) is a good candidate because of their multipotent nature The use of MSCs for cartilage repair relies on the homing and engraftment of the cells ... transforming growth factor beta-3 (TGFβ3), bone morphogenetic protein (BMP4), vitronectin (VTN) Conclusion: As I showed in the previous chapter, injection of stem cells in the knee is a promising method ... biochemical factors essential in mimicking physiological conditions for cells after administration Key words: Mesenchymal stem cell (MSC), cell migration, microfluidic device, cartilage, tissue...

Ngày tải lên: 10/09/2015, 09:11

... expressed in bone marrow- derived or synovium -derived cells Genes whose mRNA expression is differentially expressed in bone marrow- derived or synovium -derived cells Gene Upregulated in bone marrow- derived ... whereas it was negative Figure derived adherent cells Immunosuppressive properties of bone marrow- derived and synoviumderived adherent cells (a) Proliferative activity of T cells in a mixed lymphocyte ... out of six different samples of bone marrow mesenchymal stem cells (three normal, two osteoarthritis and one rheumatoid arthritis) and synovium mesenchymal stem cells (two rheumatoid arthritis,...

Ngày tải lên: 09/08/2014, 07:20

... may provide sufficient seeding cells for tissue engineering applications Bone marrow stromal cells (MSCs) are multipotent stem cells originating from the bone marrow stroma, and represent a particularly ... osteogenic and endothelial cells Methods Cell Culture and Induction Bone marrow cells were obtained from male BALB/c mice (6–8 weeks of age) Low-density bone marrow mononuclear cells (MNCs) were isolated ... [5,6] In contrast, bone marrow- derived endothelial progenitor cells (EPCs) possess high potential for neovascularization and reendothelialization [7] EPCs isolated from bone marrow or peripheral...

Ngày tải lên: 20/06/2014, 01:20

... bone defects J Bone Joint Surg Am 1998, 80, 985-996 Chang YJ, Shih DT, Tseng CP, Hsieh TB, Lee DC, Hwang SM Disparate mesenchyme-lineage tendencies in mesenchymal stem cells from human bone marrow ... 12 Kadiyala S, Jaiswal N, Bruder SP Culture-expanded, bone marrow- derived mesenchymal stem cells can regenerate a critical-sized segmental bone defect Tissue Eng 1997, 3, 173-185 13 Kadiyala S, ... umbilical cord blood Stem Cells 2006, 24, 679-685 Chen L, Tredget EE, Wu PYG, Wu Y Paracrine factors of mesenchymal stem cells recruit macrophages and endothelial lineage cells and enhance wound...

Ngày tải lên: 07/08/2014, 23:22

... Currently, several candidates of stem cells, including embryonic stem cell, neuron stem cells, bone marrow- derived mesenchymal stem cell, and peripheral blood -derived stem cells have been frequently ... adipose -derived mesenchymal stem cells (ADMSCs) for the treatment of stroke has seldom been discussed [19] Compared with embryonic stems cells and bone marrow- derived mesenchymal stem cells, ADMSCs ... transplanted cells into the host tissue [3] Interestingly, while stem cell therapy, including bone marrow- derived mesenchymal stem cells [15-17], embryonic stem cells [14] and endothelial progenitor cells...

Ngày tải lên: 18/06/2014, 16:20

... integration of the transplanted cells and the host tissue As compared with embryonic stems cells and bone marrow- derived mesenchymal stem cells, adiposederived mesenchymal stem cells (ADMSCs) have the ... Previous study has also demonstrated a therapeutic superiority of ADMSCs over bone marrowderived mesenchymal stem cells in an animal model of liver injury [14] It is, therefore, conceivable that ... (ADMSC-treated) showing few troponin I-positive myogenic-like cells (pink arrows) and undifferentiated adipose -derived mesenchymal stem cells (ADMSCs) (yellow arrows) in LV myocardium Merged image...

Ngày tải lên: 18/06/2014, 16:20

... reported [23] Besides, although bone marrowderived MSC is the major source of stem cells in these studies [22,24-27], the therapeutic role of adiposederived mesenchymal stem cells (ADMSCs) in acute IR ... endotoxin-induced systemic response by bone marrow- derived mesenchymal stem cells in mice Am J Physiol Lung Cell Mol Physiol 2007, 293:L131-141 23 Salgar S, Manning E, Ruiz P, Pham S: Mesenchymal Stem Cell ... 2010, 17:92 21 Rojas M, Xu J, Woods CR, Mora AL, Spears W, Roman J, Brigham KL: Bone marrow- derived mesenchymal stem cells in repair of the injured lung Am J Respir Cell Mol Biol 2005, 33:145-152...

Ngày tải lên: 18/06/2014, 22:20

Ngày tải lên: 07/08/2014, 23:22

... [1,8] Adult stem cells are known to have a limited differentiation potential while embryonic stem cells are totipotent Multipotent stem cells were first isolated from adult bone marrow [17] The ... anti-canine CD45, mouse anti-canine 49b, mouse anti-canine CD 51/61, mouse anti-canine CD62p, mouse anti-canine CD73, mouse anti-canine CD90, mouse anti-canine CD105, mouse anti-canine CD133, mouse anti-canine ... CD34(+) bone marrow cells Blood 2000, 95, 581-585 Bieback K, Kern S, Klüter H, Eichler H Critical parameters for the isolation of mesenchymal stem cells from umbilical cord blood Stem Cells 2004,...

Ngày tải lên: 07/08/2014, 23:22

... annulus cells adiposederived mesenchymal stem of AD-MSCfor 3D co cultured alone Increase in separate culture cells (AD-MSC) and annulus cells comderived mesenchymal stem cells (AD-MSC) and annulus cells ... sand rat adipose -derived mesenchymal extracellular 3D factor in the Immunohistochemical documentation ofstem cellsgrowthculture beta sand rat adipose -derived mesenchymal stem cells in 3D culture ... nucleus pulposus cells could secret soluble factors to direct stem cells towards the nucleus pulposus phenotype [41] Previous work on interactions of adult mesenchymal stem cells and disc cells by Le...

Ngày tải lên: 09/08/2014, 10:23