osteogenic differentiation strategies for adipose derived mesenchymal stem cells
Báo cáo y học: "TNF inhibits production of stromal cell-derived factor 1 by bone stromal cells and increases osteoclast precursor mobilization from bone marrow to peripheral blood" ppt
... hematopoietic stem cells from the bone marrow into the bloodstream of patients with a variety of malignancies The stem cells are then harvested from the blood as a source of stem cells to be returned ... cell -derived factor osteoclast precursor migration and and differentiation (a) Wild-type bone marrow cells were cultured Effect of stromal cell -derived factor on on osteoclast precursor migrationdifferentiation ... lower chamber for hours Nonmigrated cells from the upper chamber and migrated cells from the lower chamber were cultured with M-CSF and RANKL to form osteoclasts TRAP staining was formed Bar graphs,...
- 10
- 386
- 0
Báo cáo y học: "Pro-inflammatory properties of stromal cell-derived factor-1 (CXCL12) in collagen-induced arthritis" pptx
... AMD3100 at a constant rate of 600 µg/day for 14 days Histology Fore and hind limbs (ankles and interphalanges) were fixed in 10% formalin and decalcified with formic acid Paraffin sections were haematoxylin ... without CXCL12 Assays for in vivo leukocyte migration and for in vitro chemotaxis For the in vivo assay, mice were treated with AMD3100 or PBS as described above The assay was performed on the last ... 106 cells were Migrated cells were incubated with anti-CD16/CD32 Fcblocking antibodies (BD Biosciences Pharmingen, San Diego, CA, USA) and washed with PBS After washing, the cells were stained for...
- 13
- 356
- 0
Báo cáo khoa học: Syndecan-4 is a signaling molecule for stromal cell-derived factor-1 (SDF-1)/ CXCL12 pptx
... SDF1 on HeLa cells Untreated HeLa cells (A), heparitinases I- and III-treated HeLa cells (B), mock-transfected HeLa cells (C), or SD-4 dsRNA transfected HeLa cells (D) were loaded for 30 with ... incubated for or 30 with 3, 50 or 125 nm of SDF-1, and significant if the cells were incubated for 10 with 125 nm SDF-1 (Fig 4B, lanes vs 2, and data not shown) These latter conditions were therefore ... induced by SDF-1 on HeLa cells (A) Upper panel: HeLa cells were either stimulated (+) (lanes and 4) or not (–) (lanes and 3) for 10 with 125 nM SDF-1a In some experiments, cells were pretreated...
- 15
- 423
- 0
Báo cáo sinh học: "Use of recombinant lentivirus pseudotyped with vesicular stomatitis virus glycoprotein G for efficient generation of human anti-cancer chimeric T cells by transduction of human peripheral blood lymphocytes in vitro" pot
... viral titer produced by PT67 packaging cells was 106 transforming units (TU)/ml when titrated in 293 cells This titer was adequate for infecting all MD45 cells but in contrast, 106 TU/ml was not ... tumors weeks after systemic administration of Tbodies (** p < 0.01) Control cells had little effect The effect was specific for CEA as CEA- (COLO 320) cells were not affected by any cells transferred ... prospect for generating anti-cancer chimeric T cells for use in cancer immunotherapy The target selected in the current work was CEA, a tumor-selective antigen Several possible mechanisms for tumor...
- 10
- 435
- 0
báo cáo hóa học:" Use of recombinant lentivirus pseudotyped with vesicular stomatitis virus glycoprotein G for efficient generation of human anti-cancer chimeric T cells by transduction of human peripheral blood lymphocytes in vitro" docx
... viral titer produced by PT67 packaging cells was 106 transforming units (TU)/ml when titrated in 293 cells This titer was adequate for infecting all MD45 cells but in contrast, 106 TU/ml was not ... tumors weeks after systemic administration of Tbodies (** p < 0.01) Control cells had little effect The effect was specific for CEA as CEA- (COLO 320) cells were not affected by any cells transferred ... prospect for generating anti-cancer chimeric T cells for use in cancer immunotherapy The target selected in the current work was CEA, a tumor-selective antigen Several possible mechanisms for tumor...
- 10
- 411
- 0
Báo cáo y học: "Mesenchymal stem cell derived hematopoietic cells are permissive to HIV-1 infection" pptx
... used to plot the graphs Abbreviations MSCs: mesenchymal stem cells; ASCs: adipose tissue derived mesenchymal stem cells; HD: hematopoietic differentiated cells; AIDS: acquired immune deficiency syndrome; ... multipotent mesenchymal stem cells from the bone marrow of rhesus macaques Stem Cells Dev 2005, 14:440-451 doi:10.1186/1742-4690-8-3 Cite this article as: Nazari-Shafti et al.: Mesenchymal stem cell derived ... exposure on HD cells Clonally expanded cells were allowed to differentiate into HD cells for (5HD) or (8-HD) days in differentiation media For analyzing the infectivity of HD cells, we exposed...
- 12
- 364
- 0
Cloning of medaka glial cell derived neurotrophic factor (GDNF) and its receptor GFR alpha 1
... CHAPTER 1: INTRODUCTION 1.1 Spermatogonial Stem Cells (SSCs) Differentiation of germ cells in the testis originates from a constantly renewed small pool of stem cells, termed SSCs located at the basement ... mM) 0.15 µl Taq DNA polymerase (Fermentas) 14 PCR was run for 25 to 40 cycles at 94°C for 15 sec, 56°C for 15 sec and 72°C for 60 sec For RACE, PCR was carried out according to manufacturer’s ... PCR was run for 35 cycles at 94°C for 20 sec, 68°C for 20 sec and 72°C for mins instead Reagents and enzymes needed were purchased from Fermentas and thermocyclers from Applied Biosystem 2.5 Agarose...
- 48
- 214
- 0
báo cáo hóa học:" Anti-tumor activity of patient-derived NK cells after cell-based immunotherapy – a case report" doc
... Flow cytometric analysis of tumor and effector cells For flow cytometry of tumor cells, × 105 propidium iodide negative (viable) cells were incubated for 30 at 4°C in the dark with the following ... tumor cells were used Briefly, either effector or tumor cells were incubated with the relevant antibodies at a final concentration of μg/ml for 20 at 4°C Then the cells were used as targets for ... positively stained cells and the mfi in the TKD/IL-2-activated effector cells derived from leukapheresis was elevated from (21) to 3% (151) for NKp30, from 0.4 (15) to 1% (175) for NKp44, and from...
- 18
- 541
- 0
Báo cáo sinh học: "Functional characterization of human Cd33+ And Cd11b+ myeloid-derived suppressor cell subsets induced from peripheral blood mononuclear cells co-cultured with a diverse set of human tumor cell lines" docx
... Chen SH, Pan PY: Development and function of myeloid -derived suppressor cells generated from mouse embryonic and hematopoietic stem cells Stem Cells 2010, 28:620-632 Poschke I, Mougiakakos D, Hansson ... culture flasks for one week Tumor-educated myeloid (CD33+) cells were then isolated, checked for viability, and tested for suppressive function by coculture with fresh, autologous T cells in the ... CD11b+ cells with autologous T cells, respectively * indicates statistically significant suppression of T cells by CD11b+ cells from co-culture (p
- 20
- 575
- 0
Báo cáo sinh học: "Autologous Transplantation of Adipose-Derived Mesenchymal Stem Cells Markedly Reduced Acute Ischemia-Reperfusion Lung Injury in a Rodent Model" potx
... [23] Besides, although bone marrowderived MSC is the major source of stem cells in these studies [22,24-27], the therapeutic role of adiposederived mesenchymal stem cells (ADMSCs) in acute IR injury ... endotoxin-induced systemic response by bone marrow -derived mesenchymal stem cells in mice Am J Physiol Lung Cell Mol Physiol 2007, 293:L131-141 23 Salgar S, Manning E, Ruiz P, Pham S: Mesenchymal Stem Cell ... Lee JW: Therapeutic potential of mesenchymal stem cells for severe acute lung injury Chest 2010, 138:965-972 20 Chistiakov DA: Endogenous and exogenous stem cells: a role in lung repair and use...
- 13
- 280
- 0
Báo cáo khoa học: "Functional recovery and neural differentiation after transplantation of allogenic adipose-derived stem cells in a canine model of acute spinal cord injury" potx
... Safford KM, Rice HE Stem cell therapy for neurologic disorders: therapeutic potential of adipose- derived stem cells Curr Drug Targets 2005, 6, 57-62 33 Satake K, Lou J, Lenke LG Migration of mesenchymal ... human adipose tissue -derived stromal cells after cerebral ischemia in rats Exp Neurol 2003, 183, 355-366 Kang SK, Shin MJ, Jung JS, Kim YG, Kim CH Autologous adipose tissue -derived stromal cells for ... EW, Park YH Soluble factors-mediated immunomodulatory effects of canine adipose tissue -derived mesenchymal stem cells Stem Cells Dev 2008, 17, 681-693 Kang SK, Lee DH, Bae YC, Kim HK, Baik SY,...
- 12
- 309
- 0
Báo cáo khoa học: "Regeneration of human bones in hip osteonecrosis and human cartilage in knee osteoarthritis with autologous adipose-tissue-derived stem cells: a case series" docx
... implanted, autologous mesenchymal stem cells Pain Physician 2008, 11:343-353 Lee JS, Lee JS, Roh HL, Kim CH, Jung JS, Suh KT: Alterations in the differentiation ability of mesenchymal stem cells in patients ... 367(Suppl):S244-S253 Barry FP: Mesenchymal stem cell therapy in joint disease Novartis Found Symp 2003, 249:86-96 Zhang HN, Li L, Leng P, Wang YZ, Lv CY: Uninduced adipose- derived stem cells repair the defect ... Orthor Res 2006, 24:604-609 Nuttelman CR, Tripodi MC, Anseth KS: In vitro osteogenic differentiation of human mesenchymal stem cells photoencapsulated in PEG hydrogels J Biomed Mater Res A 2004, 68:773-782...
- 8
- 293
- 0
Báo cáo y học: " Alteration of T cell immunity by lentiviral transduction of human monocyte-derived dendritic cells" pdf
... DCs, from 10,000 cells per well to 313 cells per well, with × 105 allogeneic CD4 T cells in a 96-well U-bottomed plate in a total volume of 200 µl for days The proliferation of T cells was monitored ... (GFPi) For positive control, we incubated DCs with soluble IFN-γ as previously described After the DCs were co-cultured with naïve T cells for 10 days, the T cells were reactivated and analyzed for ... the cells were resuspended and fixed in 1% paraformaldehyde in PBS and analyzed using a FACSCalibur flow cytometer and the CELLQUEST program (Becton Dickinson) The live cells were gated by forward-...
- 12
- 230
- 0
Báo cáo y học: "Transcriptomic and phenotypic analysis of murine embryonic stem cell derived BMP2+ lineage cells: an insight into mesodermal patterning" ppt
... smooth muscle cells after differentiation of the BMP2+ cells Figure Detection of smooth muscle cells after differentiation of the BMP2+ cells (a) Expression of SMA in the BMP2+ cells detected ... NCSCs in stemness and to enable ongoing differentiation of NCSCs to form melanocytes Thus, the study of these BMP2-expressing cells during early differentiation of ES cells will pave the way for a ... expression (e) Figure Differentiation of the BMP2+ cells to cardiac cells Differentiation of the BMP2+ cells to cardiac cells (a) RT-PCR analysis of the cardiac markers in BMP2+ cells and other controls...
- 28
- 288
- 0
Báo cáo y học: "Analysis of proteomic profiles and functional properties of human peripheral blood myeloid dendritic cells, monocyte-derived dendritic cells and the dendritic cell-like KG-1 cells reveals distinct characteristic" doc
... KG-1 cells comment Stimulate with PMA + ionomycin for 24hrs PBMCs CD19+ve cells = waste CD19 depletion DC-like KG-1 cells (immature) CD19 –ve cells reviews CD1c selection CD1c-ve cells CD14-ve cells ... with cells of the adaptive immune system (for example, T cells) is critical for initiating immune responses and maintaining tolerance [1] DCs exist in two stages of maturation Immature cells ... the cross-talk with T cells [31,32] and other cells of the innate immune system (for example, natural killer cells) , as well as in exerting Genome Biology 2007, 8:R30 information Our finding...
- 13
- 417
- 0
Use of IPs cell derived neural stem cells as a cellular vehicle for glioma and breast cancer therapy
... for glioma and breast cancer 22 1.4 Stem cells 1.4.1 Adult stem cells 1.4.1.1 Neural stem cells 1.4.2 Induced pluripotent stem cells 1.5 Stem cell as a vehicle for cancer gene therapy 1.5.1 Stem ... induced pluripotent stem cell derived- neural stem cell human induced pluripotent stem cell derived- neural stem cell expressing CodA human induced pluripotent stem cell derived- neural stem cell expressing ... al., 2001) Stem cells as delivery vehicles have been shown to enhance gene delivery efficiency (elaborated in section 3.1) 1.4 Stem cells 1.4.1 Adult stem cells (ASCs) Adult stem cells (ASCs),...
- 134
- 439
- 0
Gene targeting in human pluripotent cell derived neural stem cells for the study and treatment of neurological disorders
... Introduction 1.1 Stem cells Stem cells have the remarkable potential to differentiate into specialized cells and thereby are key factors for the development of the whole organism All types of stem cells ... air humidity In vitro differentiation of hPS cells (hES and iPS cells) into long term proliferating pluripotent stem cell -derived neuroepithelial stem (lt-NES) cells was performed as described ... 1.1 Stem cells 1.1.1 Human pluripotent stem cells 1.1.2 In vitro differentiation potential of human pluripotent and neural stem cells 1.2 ...
- 135
- 365
- 0
Tài liệu Báo cáo Y học: Uncoupling of protein-3 induces an uncontrolled uncoupling of mitochondria after expression in muscle derived L6 cells ppt
... After 48 h, the cells were washed three times in NaCl/Pi, then fixed in 4% (w/v) paraformaldehyde/NaCl/Pi for 60 The cells were washed four times in NaCl/Pi and then incubated for 60 in NaCl/Pi ... permeabilize the plasma membrane This was performed by incubating L6 cells at a concentration of · 106 cells mL)1 into NaCl/Pi containing 50 lM digitonin on ice for Cells were washed once in buffer A (1 ... Localization of hUCP3 in L6 cells by immunofluorescence L6 cells were seeded at a density of 1.5 · 104 cells cm)2 Infection was carried out 24 h later by incubating the cells for h in the presence of...
- 9
- 467
- 0
Tìm thêm: hệ việt nam nhật bản và sức hấp dẫn của tiếng nhật tại việt nam xác định các mục tiêu của chương trình khảo sát các chuẩn giảng dạy tiếng nhật từ góc độ lí thuyết và thực tiễn khảo sát chương trình đào tạo gắn với các giáo trình cụ thể điều tra đối với đối tượng giảng viên và đối tượng quản lí khảo sát các chương trình đào tạo theo những bộ giáo trình tiêu biểu nội dung cụ thể cho từng kĩ năng ở từng cấp độ xác định mức độ đáp ứng về văn hoá và chuyên môn trong ct phát huy những thành tựu công nghệ mới nhất được áp dụng vào công tác dạy và học ngoại ngữ mở máy động cơ rôto dây quấn các đặc tính của động cơ điện không đồng bộ hệ số công suất cosp fi p2 đặc tuyến hiệu suất h fi p2 đặc tuyến mômen quay m fi p2 đặc tuyến dòng điện stato i1 fi p2 động cơ điện không đồng bộ một pha sự cần thiết phải đầu tư xây dựng nhà máy phần 3 giới thiệu nguyên liệu từ bảng 3 1 ta thấy ngoài hai thành phần chủ yếu và chiếm tỷ lệ cao nhất là tinh bột và cacbonhydrat trong hạt gạo tẻ còn chứa đường cellulose hemicellulose chỉ tiêu chất lượng 9 tr 25