osteogenic differentiation strategies for adipose derived mesenchymal stem cells

... hematopoietic stem cells from the bone marrow into the bloodstream of patients with a variety of malignancies The stem cells are then harvested from the blood as a source of stem cells to be returned ... cell -derived factor osteoclast precursor migration and and differentiation (a) Wild-type bone marrow cells were cultured Effect of stromal cell -derived factor on on osteoclast precursor migrationdifferentiation ... lower chamber for hours Nonmigrated cells from the upper chamber and migrated cells from the lower chamber were cultured with M-CSF and RANKL to form osteoclasts TRAP staining was formed Bar graphs,...

Ngày tải lên: 09/08/2014, 10:23

... AMD3100 at a constant rate of 600 µg/day for 14 days Histology Fore and hind limbs (ankles and interphalanges) were fixed in 10% formalin and decalcified with formic acid Paraffin sections were haematoxylin ... without CXCL12 Assays for in vivo leukocyte migration and for in vitro chemotaxis For the in vivo assay, mice were treated with AMD3100 or PBS as described above The assay was performed on the last ... 106 cells were Migrated cells were incubated with anti-CD16/CD32 Fcblocking antibodies (BD Biosciences Pharmingen, San Diego, CA, USA) and washed with PBS After washing, the cells were stained for...

Ngày tải lên: 09/08/2014, 07:20

... SDF1 on HeLa cells Untreated HeLa cells (A), heparitinases I- and III-treated HeLa cells (B), mock-transfected HeLa cells (C), or SD-4 dsRNA transfected HeLa cells (D) were loaded for 30 with ... incubated for or 30 with 3, 50 or 125 nm of SDF-1, and significant if the cells were incubated for 10 with 125 nm SDF-1 (Fig 4B, lanes vs 2, and data not shown) These latter conditions were therefore ... induced by SDF-1 on HeLa cells (A) Upper panel: HeLa cells were either stimulated (+) (lanes and 4) or not (–) (lanes and 3) for 10 with 125 nM SDF-1a In some experiments, cells were pretreated...

Ngày tải lên: 16/03/2014, 18:20

... viral titer produced by PT67 packaging cells was 106 transforming units (TU)/ml when titrated in 293 cells This titer was adequate for infecting all MD45 cells but in contrast, 106 TU/ml was not ... tumors weeks after systemic administration of Tbodies (** p < 0.01) Control cells had little effect The effect was specific for CEA as CEA- (COLO 320) cells were not affected by any cells transferred ... prospect for generating anti-cancer chimeric T cells for use in cancer immunotherapy The target selected in the current work was CEA, a tumor-selective antigen Several possible mechanisms for tumor...

Ngày tải lên: 19/06/2014, 08:20

... viral titer produced by PT67 packaging cells was 106 transforming units (TU)/ml when titrated in 293 cells This titer was adequate for infecting all MD45 cells but in contrast, 106 TU/ml was not ... tumors weeks after systemic administration of Tbodies (** p < 0.01) Control cells had little effect The effect was specific for CEA as CEA- (COLO 320) cells were not affected by any cells transferred ... prospect for generating anti-cancer chimeric T cells for use in cancer immunotherapy The target selected in the current work was CEA, a tumor-selective antigen Several possible mechanisms for tumor...

Ngày tải lên: 20/06/2014, 04:20

... used to plot the graphs Abbreviations MSCs: mesenchymal stem cells; ASCs: adipose tissue derived mesenchymal stem cells; HD: hematopoietic differentiated cells; AIDS: acquired immune deficiency syndrome; ... multipotent mesenchymal stem cells from the bone marrow of rhesus macaques Stem Cells Dev 2005, 14:440-451 doi:10.1186/1742-4690-8-3 Cite this article as: Nazari-Shafti et al.: Mesenchymal stem cell derived ... exposure on HD cells Clonally expanded cells were allowed to differentiate into HD cells for (5HD) or (8-HD) days in differentiation media For analyzing the infectivity of HD cells, we exposed...

Ngày tải lên: 13/08/2014, 01:20

... CHAPTER 1: INTRODUCTION 1.1 Spermatogonial Stem Cells (SSCs) Differentiation of germ cells in the testis originates from a constantly renewed small pool of stem cells, termed SSCs located at the basement ... mM) 0.15 µl Taq DNA polymerase (Fermentas) 14 PCR was run for 25 to 40 cycles at 94°C for 15 sec, 56°C for 15 sec and 72°C for 60 sec For RACE, PCR was carried out according to manufacturer’s ... PCR was run for 35 cycles at 94°C for 20 sec, 68°C for 20 sec and 72°C for mins instead Reagents and enzymes needed were purchased from Fermentas and thermocyclers from Applied Biosystem 2.5 Agarose...

Ngày tải lên: 03/10/2015, 20:57

... Flow cytometric analysis of tumor and effector cells For flow cytometry of tumor cells, × 105 propidium iodide negative (viable) cells were incubated for 30 at 4°C in the dark with the following ... tumor cells were used Briefly, either effector or tumor cells were incubated with the relevant antibodies at a final concentration of μg/ml for 20 at 4°C Then the cells were used as targets for ... positively stained cells and the mfi in the TKD/IL-2-activated effector cells derived from leukapheresis was elevated from (21) to 3% (151) for NKp30, from 0.4 (15) to 1% (175) for NKp44, and from...

Ngày tải lên: 18/06/2014, 15:20

... Chen SH, Pan PY: Development and function of myeloid -derived suppressor cells generated from mouse embryonic and hematopoietic stem cells Stem Cells 2010, 28:620-632 Poschke I, Mougiakakos D, Hansson ... culture flasks for one week Tumor-educated myeloid (CD33+) cells were then isolated, checked for viability, and tested for suppressive function by coculture with fresh, autologous T cells in the ... CD11b+ cells with autologous T cells, respectively * indicates statistically significant suppression of T cells by CD11b+ cells from co-culture (p

Ngày tải lên: 18/06/2014, 19:20

... [23] Besides, although bone marrowderived MSC is the major source of stem cells in these studies [22,24-27], the therapeutic role of adiposederived mesenchymal stem cells (ADMSCs) in acute IR injury ... endotoxin-induced systemic response by bone marrow -derived mesenchymal stem cells in mice Am J Physiol Lung Cell Mol Physiol 2007, 293:L131-141 23 Salgar S, Manning E, Ruiz P, Pham S: Mesenchymal Stem Cell ... Lee JW: Therapeutic potential of mesenchymal stem cells for severe acute lung injury Chest 2010, 138:965-972 20 Chistiakov DA: Endogenous and exogenous stem cells: a role in lung repair and use...

Ngày tải lên: 18/06/2014, 22:20

... Safford KM, Rice HE Stem cell therapy for neurologic disorders: therapeutic potential of adipose- derived stem cells Curr Drug Targets 2005, 6, 57-62 33 Satake K, Lou J, Lenke LG Migration of mesenchymal ... human adipose tissue -derived stromal cells after cerebral ischemia in rats Exp Neurol 2003, 183, 355-366 Kang SK, Shin MJ, Jung JS, Kim YG, Kim CH Autologous adipose tissue -derived stromal cells for ... EW, Park YH Soluble factors-mediated immunomodulatory effects of canine adipose tissue -derived mesenchymal stem cells Stem Cells Dev 2008, 17, 681-693 Kang SK, Lee DH, Bae YC, Kim HK, Baik SY,...

Ngày tải lên: 07/08/2014, 23:22

... implanted, autologous mesenchymal stem cells Pain Physician 2008, 11:343-353 Lee JS, Lee JS, Roh HL, Kim CH, Jung JS, Suh KT: Alterations in the differentiation ability of mesenchymal stem cells in patients ... 367(Suppl):S244-S253 Barry FP: Mesenchymal stem cell therapy in joint disease Novartis Found Symp 2003, 249:86-96 Zhang HN, Li L, Leng P, Wang YZ, Lv CY: Uninduced adipose- derived stem cells repair the defect ... Orthor Res 2006, 24:604-609 Nuttelman CR, Tripodi MC, Anseth KS: In vitro osteogenic differentiation of human mesenchymal stem cells photoencapsulated in PEG hydrogels J Biomed Mater Res A 2004, 68:773-782...

Ngày tải lên: 10/08/2014, 23:21

... DCs, from 10,000 cells per well to 313 cells per well, with × 105 allogeneic CD4 T cells in a 96-well U-bottomed plate in a total volume of 200 µl for days The proliferation of T cells was monitored ... (GFPi) For positive control, we incubated DCs with soluble IFN-γ as previously described After the DCs were co-cultured with naïve T cells for 10 days, the T cells were reactivated and analyzed for ... the cells were resuspended and fixed in 1% paraformaldehyde in PBS and analyzed using a FACSCalibur flow cytometer and the CELLQUEST program (Becton Dickinson) The live cells were gated by forward-...

Ngày tải lên: 13/08/2014, 13:20

... smooth muscle cells after differentiation of the BMP2+ cells Figure Detection of smooth muscle cells after differentiation of the BMP2+ cells (a) Expression of SMA in the BMP2+ cells detected ... NCSCs in stemness and to enable ongoing differentiation of NCSCs to form melanocytes Thus, the study of these BMP2-expressing cells during early differentiation of ES cells will pave the way for a ... expression (e) Figure Differentiation of the BMP2+ cells to cardiac cells Differentiation of the BMP2+ cells to cardiac cells (a) RT-PCR analysis of the cardiac markers in BMP2+ cells and other controls...

Ngày tải lên: 14/08/2014, 08:20

... KG-1 cells comment Stimulate with PMA + ionomycin for 24hrs PBMCs CD19+ve cells = waste CD19 depletion DC-like KG-1 cells (immature) CD19 –ve cells reviews CD1c selection CD1c-ve cells CD14-ve cells ... with cells of the adaptive immune system (for example, T cells) is critical for initiating immune responses and maintaining tolerance [1] DCs exist in two stages of maturation Immature cells ... the cross-talk with T cells [31,32] and other cells of the innate immune system (for example, natural killer cells) , as well as in exerting Genome Biology 2007, 8:R30 information Our finding...

Ngày tải lên: 14/08/2014, 18:20

... for glioma and breast cancer 22 1.4 Stem cells 1.4.1 Adult stem cells 1.4.1.1 Neural stem cells 1.4.2 Induced pluripotent stem cells 1.5 Stem cell as a vehicle for cancer gene therapy 1.5.1 Stem ... induced pluripotent stem cell derived- neural stem cell human induced pluripotent stem cell derived- neural stem cell expressing CodA human induced pluripotent stem cell derived- neural stem cell expressing ... al., 2001) Stem cells as delivery vehicles have been shown to enhance gene delivery efficiency (elaborated in section 3.1) 1.4 Stem cells 1.4.1 Adult stem cells (ASCs) Adult stem cells (ASCs),...

Ngày tải lên: 09/09/2015, 18:56

... Introduction 1.1 Stem cells Stem cells have the remarkable potential to differentiate into specialized cells and thereby are key factors for the development of the whole organism All types of stem cells ... air humidity In vitro differentiation of hPS cells (hES and iPS cells) into long term proliferating pluripotent stem cell -derived neuroepithelial stem (lt-NES) cells was performed as described ...   1.1   Stem cells   1.1.1   Human pluripotent stem cells   1.1.2   In vitro differentiation potential of human pluripotent and neural stem cells   1.2  ...

Ngày tải lên: 26/11/2015, 09:54