1 characteristics of ucb derived mesenchymal stem cells

... therapeutic role of adiposederived mesenchymal stem cells (ADMSCs) in acute IR injury of the lung has not been investigated Recently, ADMSCs have been reported to have the distinct advantages of being ... Use of Laboratory Animals (NIH publication No Page of 13 85-23, National Academy Press, Washington, DC, USA, revised 1996) Animal Grouping and Isolation of Adipose -Derived Mesenchymal Stem Cells ... parenchyma from IR damage Figure Impact of Adipose -Derived Mesenchymal Stem Cells (ADMSC) Transplantation on the Severity of IR-Induced Lung Parenchymal Injury Number of alveolar sacs and crowded area...

Ngày tải lên: 18/06/2014, 22:20

... evaluate adipogenic potential of amniotic fluid -derived human mesenchymal stem cells Stem Cells Dev 2006, 15:719-726 55 Montaln H, Schichor C, Lah TT: Human mesenchymal stem cells and their use in cell-based ... intracellular detection of a low amount of CD4 in about 20% of MSCs suggests a possible complex regulation of CD4 protein expression in these cells It is noteworthy that this pattern of CD4 expression ... infection of human bone marrow stromal fibroblasts Blood 1990, 76:317-322 40 Wang L, Mondal D, La Russa VF, Agrawal KC: Suppression of clonogenic potential of human bone marrow mesenchymal stem cells...

Ngày tải lên: 13/08/2014, 01:20

... ’slaminA yrotarobaL fo esU dna eraC eht rof ediuG‘ eht fo senilediug eht ot gnidrocca demrofrep saw noitagitsevni sihT yduts yrujni droc lanips latnemirepxe eht rof desu erew ,gk 95.0 ± 77.3 dehgiew ... wef a ,revewoh ;noitamrof ytivac fo egatnecrep tsellams eht detartsnomed puorg PC eht ,noitidda nI spuorg GBCU dna CSM-BCU eht ni noitamrof ytivac fo egatnecrep rellams a dnuof ew ,sllec mets detnalpsnart ... sllec detnalpsnart eht rof troppus reffo yam droc derujni eht ,droc lanips eht fo sdne lamixorp dna latsid eht ot noitcennoc rof ,slairetam ro sllec suoirav fo pu edam dloffacs ro egdirb a htiw...

Ngày tải lên: 07/08/2014, 23:22

... umbilical cord blood Stem Cells 2006, 24, 679-685 Chen L, Tredget EE, Wu PYG, Wu Y Paracrine factors of mesenchymal stem cells recruit macrophages and endothelial lineage cells and enhance wound ... human mesenchymal stem- cell-induced bone formation Biomaterials 2005, 26, 3631-3638 Bruder SP, Kraus KH, Goldberg VM, Kadiyala S The effect of implants loaded with autologous mesenchymal stem cells ... of mixture of β-TCP and cells revealed significantly greater area of new bone formation than control (p ＜ 0.05) and a smaller amount of residual TCP Previous studies showed that bone marrow-derived...

Ngày tải lên: 07/08/2014, 23:22

... immunophenotype of cUCB-MSCs expressed mesenchymal stem cells surface markers such as CD29, CD44 and CD105 However, the cUCB-MSCs had negative expressions of hematopoietic surface markers of CD14, CD34 ... study, we used cUCB-MSCs at 3∼5 passage Generally, increasing the passage number of adult stem cells often leads to a decline in the multipotent abilities [36] Human mesenchymal stem cells could ... Chen TH Isolation of multipotent mesenchymal stem cells from umbilical cord blood Blood 2004, 103, 1669-1675 Mauney JR, Volloch V, Kaplan DL Role of adult mesenchymal stem cells in bone tissue...

Ngày tải lên: 07/08/2014, 23:22

... Journal of Biomedical Science 2010, 17:75 http://www.jbiomedsci.com/content/17/1/75 Page of 10 Figure Characterization of bone marrow -derived MSCs Flow cytometric analyses of bone marrow -derived ... of importance for the anti-apoptotic role of simvastatin Effect of simvastatin on the VEGF releasing of bone marrow -derived MSCs in vitro To assess whether simvastatin affected the release of ... Marshak DR: Multilineage potential of adult human mesenchymal stem cells Science 1999, 284:143-147 Dimmeler S, Leri A: Aging and disease as modifiers of efficacy of cell therapy Circ Res 2008, 102:1319-1330...

Ngày tải lên: 10/08/2014, 05:21

... engineering by providing abundant seeding cells with high quality List of abbreviations ADSCs: adipose -derived mesenchymal stem cells; MSCs: mesenchymal stem cells; ALP: alkaline phosphatase; DT: ... adipose -derived stem cells Cryobiology 2008, 57:18-24 Chen MY, Lie PC, Li ZL, Wei X: Endothelial differentiation of Wharton’s jelly -derived mesenchymal stem cells in comparison with bone marrowderived mesenchymal ... osteogenesis and vascularization of mesenchymal stem cells Cells Tissues Organs 2009, 190:185-193 Rodbell M: The metabolism of isolated fat cells IV Regulation of release of protein by lipolytic hormones...

Ngày tải lên: 10/08/2014, 10:20

... the treatment of ALI Among the various sources of stem cells, human umbilical cord blood (UCB) provides readily available MSCs with low immunogenicity [11-13] Therefore, human UCB- derived MSCs ... downregulation of E coli-induced ALI by intratracheal transplantation of human UCB- derived MSCs was clearly associated with the control of complex inflammatory interactions Human UCB- derived MSCs ... Potential application of mesenchymal stem cells in acute lung injury Expert Opin BIol Ther 2009, 9:1259-1270 Matthay MA, Goolaerts A, Howard JP, Lee JW: Mesenchymal stem cells for acute lung injury:...

Ngày tải lên: 12/08/2014, 13:22

... Types of cells used in bone tissue engineering for osteogenic differentiation 59 2.3.1 Potential of mesenchymal stem cells (MSCs) for bone healing 60 2.3.2 Potential of bone marrow derived mesenchymal ... Casey K Chan Effects of mechanical stimulation in osteogenic and chondrogenic differentiation of bone marrow -derived mesenchymal stem cells on nanofibrous scaffolds Journal of Bioactive and Compactable ... inflammatory cells, cells of mesenchymal origin Chemotactic effect on other inflammatory cells, stimulation of extracellular matrix synthesis, angiogenesis, recruitment of endogenous fibrogenic cells...

Ngày tải lên: 11/09/2015, 09:57

... Isolation of mesenchymal stem cells of fetal or maternal origin from human placenta Stem Cells 22, 1338–1345 Bieback K, Kern S, Kluter H & Eichler H (2004) Critical parameters for the isolation of mesenchymal ... evidence of transdifferentiation by MET in the process of hepatic differentiation of AT-MSCs No previous report has demonstrated evidence of transdifferentiation of committed adult stem cells In ... differentiation of human umbilical cord blood -derived mesenchymal stem cells into hepatocytelike cells Biochem Biophys Res Commun 330, 1153–1161 Seo MJ, Suh SY, Bae YC & Jung JS (2005) Differentiation of...

Ngày tải lên: 30/03/2014, 04:20

... new potential source of pluripotent cells for regenerative medicine The identification of niches of tissue-specific stem cells capable of replacing damaged differentiated cells in the hFTs may ... Prockop D: Mesenchymal stem/ multipotent stromal cells: the state of transdifferentiation and modes of tissue repair – current views Stem Cells 2007, 25:2896-28902 Valtieri M, Sorrentino A: The mesenchymal ... Vainzof M, Zatz M: SJL dystrophic mice express a significant amount of human muscle proteins following systemic delivery of human adipose -derived stromal cells without immunosuppression Stem Cells...

Ngày tải lên: 18/06/2014, 15:20

... use of adipose -derived mesenchymal stem cells (ADMSCs) for the treatment of stroke has seldom been discussed [19] Compared with embryonic stems cells and bone marrow -derived mesenchymal stem cells, ... neuron stem cells, bone marrow -derived mesenchymal stem cell, and peripheral blood -derived stem cells have been frequently investigated for their feasibility and safety in the treatment of stroke ... Ratajczak J, Ratajczak MZ: Trafficking of normal stem cells and metastasis of cancer stem cells involve similar mechanisms: pivotal role of the SDF-1-CXCR4 axis Stem Cells 2005, 23:879-894 Wei YJ, Tang...

Ngày tải lên: 18/06/2014, 16:20

... As compared with embryonic stems cells and bone marrow -derived mesenchymal stem cells, adiposederived mesenchymal stem cells (ADMSCs) have the distinct advantages of being abundant, easy to obtain ... Ratajczak J, Ratajczak MZ: Trafficking of normal stem cells and metastasis of cancer stem cells involve similar mechanisms: pivotal role of the SDF-1-CXCR4 axis Stem Cells 2005, 23:879-894 Kee HJ, Sohn ... therapeutic superiority of ADMSCs over bone marrowderived mesenchymal stem cells in an animal model of liver injury [14] It is, therefore, conceivable that ADMSCs would be of tremendous momentum...

Ngày tải lên: 18/06/2014, 16:20

... whereas it was negative Figure derived adherent cells Immunosuppressive properties of bone marrow -derived and synoviumderived adherent cells (a) Proliferative activity of T cells in a mixed lymphocyte ... marrow -derived or synovium -derived cells Genes whose mRNA expression is differentially expressed in bone marrow -derived or synovium -derived cells Gene Upregulated in bone marrow -derived cells ... recently shown to play a role in the maintenance of the pluripotency of human embryonic stem cells [40] The maintenance of pluripotency of embryonic stem cells could involve Wnt signaling and could...

Ngày tải lên: 09/08/2014, 07:20

... annulus cells adiposederived mesenchymal stem of AD-MSCfor 3D co cultured alone Increase in separate culture cells (AD-MSC) and annulus cells comderived mesenchymal stem cells (AD-MSC) and annulus cells ... synthesis of proteoglycans after pellet co-culture of disc cells with BM-MSC [20] Stimulation of disc cells with stem cells or CM could enhance the success of autologous implantation of disc cells ... Immunolocalisation of the mesenchymal stem cell markers from passage one adipose -derived mesenchymal stem cells grown in monolayer (a) monolayer CD29 at × 650; (b) CD105 at × 840 Page of 10 (page number...

Ngày tải lên: 09/08/2014, 10:23

... Under most circumstance, the treatment of MI by using MSCs showed poor survival of transplanted cells In addition to the quick loss of cells within 24 h of transplantation caused by cell leakage ... nonadherent cells were removed by a medium change at 72 h and every four days thereafter After two passages, homogeneous MSCs that devoid of hematopoietic cells were used A total of × 106 cells/ ml ... Preparation of MSCs MSCs were isolated from bone marrow of adult SpragueDawley male rats and expanded according to reported protocols [2,4] Whole marrow cells were cultured at a density of × 10 cells/ cm...

Ngày tải lên: 10/08/2014, 05:21

... with Regard to the Source of Fetus -Derived Stem Cells Stem cells 2007, 25:2886-2895 Wu KH, Mo XM, Zhou B, Lu SH, Yang SG, Liu YL, Han ZC: Cardiac potential of stem cells from whole human umbilical ... differentiation of HUMSCs into cardiomyocytes and maturation of HUMSCs derived cardiomyocytes Admittedly, the detailed mechanism(s) of above effects of S1P on differentiation of and maturation of HUMSCs derived ... consisting of 500 ml of basal medium, 25 ml of fetal bovine serum (ScienCell Research Laboratories, Cat No 0025), ml of mesenchymal stem cell growth supplement (Cat No.7552) and ml of penicillin/...

Ngày tải lên: 10/08/2014, 05:21

... Osteogenic Cells 46 2.8.2.2 Mesenchymal Stem Cells 48 2.8.2.3 Mesenchymal Stem Cells in Bone Tissue Engineering .49 2.8.2.4 Proposed Cell Type – Human Fetal Mesenchymal Stem Cells ... progenitor stem cells accelerates and potentiates the osteogenic response of bone marrow derived human fetal mesenchymal stem cells through paracrine signalling mechanisms International Society for Stem ... constructs through the coculture of umbilical cord derived endothelial progenitor cells and fetal bone marrow derived mesenchymal stem cells 7th Singapore International Congress of O&G (SICOG), 2629 August...

Ngày tải lên: 09/09/2015, 10:16

... Treatment of Femoral Head Necrosis with Arterial Perfusion of Marrow Stem Cells Chin J Integr Trad West Med 2004,24(11):999-1002 Yan Z, Hang D, Guo C, Chen Z Fate of mesenchymal stem cells transplanted ... infusion of bone marrow -derived Flk-1+CD31-CD34- mesenchymal stem cells: feasibility and safety from monkey to human Stem Cells Dev,2006,15(3):349-357 Devine SM, Bartholomew AM, Mahmud N, et al Mesenchymal ... marrow -derived stromal progenitor cells (mesenchymal progenitor cells) : implications for therapeutic use Bone Marrow Transplant,1995,16(4):557-564 Aggarwal S, Pittenger MF Human mesenchymal stem cells...

Ngày tải lên: 25/10/2012, 11:18