Quantification of epidermal growth factor receptor dynamics and interactions in living cells by fluorescent correlation and cross correlation spectroscopy

... for studying rafts is interrupting rafts by changing the amount of cholesterol in cells, which includes increasing its amount by adding in extra cholesterol and extracting it by drug treatments ... thereby enabling the exposure of the ligand-binding pocket of domain L1 and L2 The dimerization arm in domain CR1 is known to facilitate the formation of dimers, which lead...

Ngày tải lên: 10/09/2015, 09:25

... Coulter Inc., Fullerton, CA, USA) according to manufacturer instructions Multiplex PCR and primer extension analysis of mutations in EGFR-216 promoter region and exons 18, 19, 20, and 21 Multiplex PCR ... amount of work and time required With this method, -216 promoter region and exons 18-21 of the EGFR gene were amplified with multiplex PCR in a sing...

Ngày tải lên: 10/08/2014, 05:21

... system may be one of the mechanisms responsible for drug -induced apoptosis in a variety of JNK activation is critical for AG1478 -induced apoptosis cancer cells of different histotype [51] Chang ... RA & Davis RJ (2000) Requirement of JNK for stress -induced activation of the cytochrome c-mediated death pathway Science 288, 870–874 JNK activation is...

Ngày tải lên: 18/02/2014, 13:20

... regulatory mechanism of MIG6 in relation to the EGFR mutation The present study is the first to show the association of MIG6 expression with the EGFR mutation in cancer MIG6 ⁄ RALT is known to be ... EGFR mutation and MIG6 expression T Nagashima et al Introduction Epidermal growth factor receptor (EGFR) is a membrane tyrosine kinase that is involved in t...

Ngày tải lên: 30/03/2014, 01:20

... Immunohistochemical staining intensity scores A) Meningiomas stained with anti-EGFR antisera showing negative staining B) 1+ staining C) 2+ staining D) 3+ staining Original magnification for ... Beguinot L, Macchia V: Epidermal growth factor receptor in human brain tumors J Endocrinol Invest 1992, 15(1):31-37 Johnson MD, Horiba M, Winnier AR, Artiaga CL: The epidermal growth...

Ngày tải lên: 09/08/2014, 09:20

... expression of the MUC5AC mucin (a predominant airway mucin) gene Interaction of EGFR with its ligands led to EGFR tyrosine kinase phosphorylation, and a selective EGFR tyrosine kinase inhibitor ... virus-induced goblet-cell metaplasia in pathogen-free rats, developing mucin- containing cells did not incorporate [3H]thymidine, suggesting that cell mitosis was not involved in synthe...

Ngày tải lên: 12/08/2014, 18:20

... difference in PFS was also found in patients treated with Cetuximab plus chemotherapy as second or more line with or without an increased EGFR-GCN (p = 0.03) In our population 89 /101 patients were treated ... EGFR -gene copy number (GCN) in 31 selected patients with metastatic CRC treated with Cetuximab or Panitumumab Eight out of nine patients who obt...

Ngày tải lên: 18/06/2014, 16:20

... Mutations of the epidermal growth factor receptor gene and related genes as determinants of epidermal growth factor receptor tyrosine kinase inhibitors sensitivity in lung cancer Cancer Sci 98, ... Kinetic analysis of epidermal growth factor receptor somatic mutant proteins shows increased sensitivity to the epidermal growth factor receptor tyrosine k...

Ngày tải lên: 16/02/2014, 09:20

... there could be a link between EGF-induced reduction in Bid and tBid levels and the ubiquitin ligase activity of Itch In this study, we first examined the ability of Itch to interact with Bid and ... proteins Bim and Bak and the C-terminal fragment of Bid The ubiquitin ligases responsible for the ubiquitylation are in most cases not known [44] Her...

Ngày tải lên: 16/02/2014, 09:20

... Nck [72] The binding of these proteins could prevent the interaction of the Ca2+ ⁄ CaM complex to the receptor if the CaMBD were occluded at least in part Nevertheless, the docking of these adaptor ... internalization and the subsequent degradation of the receptor in transfected human embryonic kidney cells [50] Direct regulation of the EGFR by CaM The dire...

Ngày tải lên: 06/03/2014, 09:22

... the epidermal growth factor (EGF) receptor ⁄ extracellular signalregulated kinase ⁄ and EGF receptor ⁄ Akt pathway for proliferation Mol Cancer Ther 3, 46 5–4 72 Amann J, Kalyankrishna S, Massion ... motility, and invasion It is also of great interest and importance to note that about one-third of the human lung cancer samples that we examined revealed significant downregulat...

Ngày tải lên: 07/03/2014, 00:20

... before the cells were washed and Hrs was immunoprecipitated from the lysate We chose to stimulate the cells with the growth factors for min, as the growth factor- stimulated phosphorylation of Hrs ... hypertonic media prevents the EGF-induced phosphorylation of Hrs This suggests that the phosphorylation of Hrs takes place on endosomes [4] As the activa...

Ngày tải lên: 17/03/2014, 11:20

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Ngày tải lên: 07/08/2014, 18:21

... signal-regulated kinases: association with epidermal growth factor receptor/ transforming growth factor alpha expression in head and neck squamous carcinoma and inhibition by anti -epidermal growth factor receptor ... transforming growth factor α and epidermal growth factor receptor in patients with squamous cell carcinoma of the head and nec...

Ngày tải lên: 09/08/2014, 10:20

... Sustained complete remission of human epidermal growth factor receptor 2-positive metastatic breast cancer in the liver during long-term trastuzumab (Herceptin) maintenance therapy in a woman: a case ... will influence whether a patient achieves long-lasting remission on maintenance trastuzumab therapy We also speculate that the...

Ngày tải lên: 11/08/2014, 02:22