transforming growth factorβ1 induced epithelialmesenchymal transition in hepatic fibrosis

... that in intact G 1 cells (subdiploid cells). For annexin-V/PI staining 1 ì 10 6 cells were resuspended in 50 àl binding buffer (Roche Diagnostics, Lewes, UK) and incu- bated with annexin-V/FITC ... physiological TGF-β 1 activation during blood clotting in patients and control individuals, and examined whether TGF-β 1 activation was associated with clinical phenotype, in particular inflammatory disease ... [19,20] involving protease (plasmin) activation and interaction of TGF-β 1 with thrombospondin-1. Using an ELISA assay validated for detection of active TGF-β 1 [15,16], we determined the increased...

Ngày tải lên: 09/08/2014, 08:22

Ngày tải lên: 11/08/2014, 08:22

... either activate or inhibit protein synthesis and cell growth [2]. The most well defined signaling mechanism regulating mTOR activity in skeletal muscle is the insulin like growth factor-1 (IGF-1) ⁄ insulin-dependent ... rapa- mycin (mTOR) plays a critical role in regulating the rate of protein synthesis and cell hypertrophy in skele- tal muscle [3–6]. mTOR is a serine ⁄ threonine kinase of the phosphatidylinositol kinase-related ... signal-regulated kinase; GAP, GTPase activating protein; GST, glutathione S-transferase; IGF-1, insulin like growth factor-1; MEK, mitogen-activated protein kinase kinase; mTOR, mammalian target of rapamycin;...

Ngày tải lên: 15/03/2014, 11:20

Ngày tải lên: 12/08/2014, 17:22

Ngày tải lên: 11/08/2014, 08:21

... of several signaling pathways in transforming growth factor-beta-1 (TGF-β1) -induced expression of the Ank gene. (a) Kinetics of sign- aling events induced by TGF-β1. Total proteins were extracted ... 1 Effect of transforming growth factor-beta-1 (TGF-β1) on proteins regu-lating inorganic pyrophosphate metabolismEffect of transforming growth factor-beta-1 (TGF-β1) on proteins regu- lating inorganic ... signal-regulated kinase; MEK-1, mitogen-activated protein kinase/extracellu- lar signal-regulated kinase kinase 1; p38-MAPK, p38-mitogen-activated protein kinase; PKA, protein kinase A; PKC, protein kinase...

Ngày tải lên: 09/08/2014, 10:22

Ngày tải lên: 12/08/2014, 11:22

... the cytokines involved in the induction of FXR1 remain un- characterized. The regulation by the anti -in ammatory cytokine transforming growth factor-b1 (TGF-b1) of the proinflammatory cytokine TNF-a ... example, in the stabilization of chemokine mRNA. TGF-b1 opposes LPS -induced chemokine stabilization by inhibiting p38 MAPK [33]. To determine whether this signalling pathway was involved in FXR1 induction, ... demonstrated that FXR1, an mRNA- binding protein, plays an essential role in this process. Building on previous work showing that TGF-b1 can inhibit LPS -induced TNF-a and chemokine production [33,35],...

Ngày tải lên: 22/03/2014, 21:21

... Clinical Pharmacology, Naples, Italy Email: Giovanni Tarantino* - tarantin@unina.it; Paolo Conca - paolo.conca@unina.it; Antonio Riccio - riccio@unina.it; Marianna Tarantino - tarantin@unina.it; ... Serum concentrations of transforming growth factor-beta1 and ferritin. Results: High concentrations of transforming growth factor-beta1 were noticed in patients suffering from both fatty liver and ... its use in clinical settings [1]. The definition of "benignity" concerning FL is wide- accepted [2] but conceptually difficult to maintain because the mechanisms, i.e., insulin resistance...

Ngày tải lên: 18/06/2014, 15:20

... doxorubicin and vincristine against Ewing’s sarcoma cells. Clin Cancer Res 2001, 7:1790-7. 30. Martins AS, Mackintosh C, Martín DH, Campos M, Hernández T, Ordóñez JL, de Alava E: Insulin-like growth ... pathway inhibition by ADW742, alone or in combination with imatinib, doxorubicin, or vincristine, is a novel therapeutic approach in Ewing tumor. Clin Cancer Res 2006, 12:3532-40. 31. Kim WY, Jin ... on IGF1R in GISTs and discuss the future perspectives in this promising field. Introduction Recent years have seen a growing interest in insulin-like growth fa ctor 1 receptor (IGF1R) in medical...

Ngày tải lên: 18/06/2014, 16:20

... reduction in inflammation in arthritic joints, TGF-β1 expression induced fibrogenesis and muscle edema. In addition, TGF-β1 expression in the ade- novirally infected synovial lining also resulted in induction of ... to confer sustained, intra-articular TGF-β1 expression in both naïve and arthritic rabbit knee joints. Intra-articular injec- tion of adenoviral vector expressing human transforming growth factor ... antigen -induced arthritis (AIA), rabbits were sensitized to ovalbumin by intradermal injections of 5 mg ovalbumin emulsified in Freund’s complete adjuvant. Arthritis was initiated in both hind knees...

Ngày tải lên: 09/08/2014, 01:21

... Mohan S, Ljunghall S: Circulating levels of insulin-like growth factor-I and -II, and IGF- binding protein-3 in inflammation and after parathyroid hormone infusion. Bone Miner 1994, 24:25-31. 33. ... Poli V, Martini A, Ciliberto G, Fattori E: Interleukin 6 causes growth impairment in transgenic mice through a decrease in insulin- like growth factor-I. A model for stunted growth in children with ... significantly during the first year in the HRT group compared with the controls. There is a need to be cautious in interpreting this finding, since there were significantly more missing OPG samples in the...

Ngày tải lên: 09/08/2014, 01:21

... 39:968-978. 26. Morales TI: The insulin-like growth factor binding proteins in uncultured human cartilage. Increases in insulin-like growth factor binding protein 3 during osteoarthritis. Arthritis ... Holly JMP: Changes in the local regulation of insulin-like growth factors I and II and insulin-like growth factor-binding proteins in osteoarthritis of the canine stifle joint secondary to cruciate ... McCarthy JR, Xie Q, Ling N, Chen C: Quino- line-carboxylic acids are potent inhibitors that inhibit the bind- ing of insulin-like growth factor (IGF) to IGF-binding proteins. Bioorg Med Chem...

Ngày tải lên: 09/08/2014, 01:23

... effector cells is underscored both in experimental models and in humans in which the numbers Review Transforming growth factor- ββ -induced regulatory T cells referee inflammatory and autoimmune diseases Sharon ... Beyond engaging Smad activity, TGF-β triggers the extracellular signal-related kinase and p38 mitogen-activated kinase pathways [44] to link additional signaling cascades involved in modulating cell ... [64,65]. Moreover, in a dramatic turnaround of allergen -induced asthmatic lung disease, TGF-β-converted /induced Treg, when transferred to an asthmatic mouse, suppressed allergen -induced inflammation...

Ngày tải lên: 09/08/2014, 06:22

... of various transforming growth factor (TGF)-beta signaling molecules in cartilageStaining of various transforming growth factor (TGF)-beta signaling molecules in cartilage. Paraffin sections ... Wang W, Dunander C, Heingard D: Inhibition of interleukin-1alpha- induced cartilage oligomeric matrix protein degradation in bovine articular cartilage by matrix metalloproteinase inhibi- tors: potential ... Boumediene K: Mediation of interleukin-1beta- induced transforming growth factor beta1 expression by acti- vator protein 4 transcription factor in primary cultures of bovine articular chondrocytes:...

Ngày tải lên: 09/08/2014, 07:20

... cartilage in aging mice [2]. This commentary will discuss TGF-β signaling and osteoarthritis in an experimental investigation in the mouse. It is important to bear in mind that results obtained in mice ... catabolic cyto- kines [1]. The aim of this commentary is to place in context a recent article in this journal of anabolic growth factor, trans- forming growth factor-β (TGF-β) signaling in articular ... protein; C-Smad = common Smad (Smad 4); IL = interleukin; LAP = latency-associated protein; MH = Mad homology domain in the Smads; R-Smad = receptor-activated Smad (Smads 2 and 3 for TGF-β signaling;...

Ngày tải lên: 09/08/2014, 07:20

... H, Oni- shi Y, Inukai K, Anai M, Fukushima Y, et al.: 14-3-3 protein binds to insulin receptor substrate-1, one of the binding sites of which is in the phosphotyrosine binding domain. J Biol Chem 1997, ... in OA Obs, unlike in normal cells. Since there is an increase in IGF-1 production in OA Obs with a concomitant decrease of the major insulin-like growth factor binding pro- teins, namely BP-3, ... activation via IRS-1 in OA Obs. We recently demon- strated that transforming growth factor (TGF)-β production is increased in OA Obs [20] and, since this growth factor can activate PKB in arthritis,...

Ngày tải lên: 09/08/2014, 08:23

... -0.531 38889_at ZFYVE9 Zinc finger, FYVE domain containing 9 (SARA) -0.538 33831_at CREBBP CREB binding protein, CBP -0.539 38311_at TGIF2 TGFB -induced factor 2 -0.559 1734_at TGFB3 Transforming growth factor, ... protease inhibitors, resulting in enhanced proteolytic activity [41] and controls cell proliferation by processing the insulin-like growth factor-binding protein-1 [42]. MMP-11 is, therefore, involved ... Red arrow indicates the cleavage site. CBP, CREB binding protein; LAP, latency-associated protein; LTBP, latent TGF-β binding protein; P, phosphate; TGFBR, TGF-β receptor; TGIF, TGFB- induced...

Ngày tải lên: 09/08/2014, 10:20

Ngày tải lên: 10/08/2014, 05:21

Ngày tải lên: 11/08/2014, 00:23