transforming growth factorβ and smads

... interacts with and influences the effects of key bone regulators insulin-like growth factor (IGF)1 and transforming growth factor (TGF)-β [93,94] STC1 Stanniocalcin inhibits calcium uptake and has inhibitory ... protein kinases (AKT3 and PRKCD), calcium and metal ion binding proteins (S100A4, S100A6, MT1L, MT2A and MT1G), transcription factors (TWIST1, FMR2, KLF6, NR4A2 and DEC1), and both enzymatic (ADAMTS4, ... insulin-like growth factor-2 and TGF-β1 in cortical bone from the iliac crest [38]; matrix metalloproteinase (MMP)2 and liver alkaline phosphatase in subchondral bone [4]; and IL-1β, IL-6 and TGF-β1

Ngày tải lên: 09/08/2014, 10:21

... contributions HEG and ENH conceived the study and participated in its design and co-ordination. HT and HEG wrote the manuscript. HT, MK and RD performed all experiments and assays. HT and RD retrieved ... bers of the transforming growth factor-beta (TGFβ) super fam- ily such as the TGFβ isoforms and the BMPs [15]. Also important are the fibroblast growth factor isoforms and insulin- like growth factor ... to increase proteoglycan and collagen type I production To increase proteoglycan and collagen type I production, 3D cell culture and exposure to TGFβ were used. Growth and differentiation of stem

Ngày tải lên: 09/08/2014, 10:23

... lysed, and stored at -80°C All tissue TGF-β1 values were then standardized by bladder weight and expressed as μg per bladder Immunostaining and Confocal Microscopy of iNOS, active TGF-β1, and latent ... Figure Urine levels of active and latent/total TGF-β1 at baseline and after CYP Urine levels of active and latent/total TGF-β1 at baseline and after CYP Active and latent/total TGF-β1 values ... contributions PT and YV designed the study DB, PL, VT and EW executed the experiments in the manuscripts NY and RZ were involved in data analysis and manuscript preparation All authors read and approved

Ngày tải lên: 11/08/2014, 08:22

... of growth factors such as Transforming Growth Factor (TGF)-β superfamily members [4,5] The TGF-β superfamily of ligands comprises more than 35 members in mammals, including TGF-β1-3, activins and ... Dorset, UK) and grown at 37°C in a humidified 5% CO2 atmosphere in fibroblast growth medium (FGM, Lonza Rockland Inc, Rockland, ME, USA) supplemented with 0.5 ml recombinant human fibroblast growth ... BMP-4 and BMP-7 (Figure 4A) BMP-4 inhibited the TGF-β1induced increase in the level of the transcripts encoding collagen type I and IV (Figures 3A and 3B), tenascin and fibronectin (Figures 4A and

Ngày tải lên: 12/08/2014, 11:22

... unrestricted use, distribution, and reproduc- tion in any medium, provided the original work is properly cited. Research article Platelet-derived growth factor and transforming growth factor beta synergistically ... Rosengren, Maripat Corr and David L Boyle* Abstract Introduction: The objective of this study was to model the effects of transforming growth factor beta (TGF-β) and platelet-derived growth factor (PDGF), ... MIP1α and MMP3), while leaving some other media- tors unaltered. Both PDGF and TGF-β induce prolifera- tion of FLS [3,5,7], and cytokine-induced growth of FLS is potentiated by PDGF [17] and TGF-β

Ngày tải lên: 12/08/2014, 12:20

... Western blotting and its regulation was assessed by addition of different protease inhibitors and transforming growth factor - β1 (TGF-β1) The role of uPAR cleavage in cell proliferation and migration ... (CAFs) and cancer cells [37–39] The aim of the current study was to analyse the regulation of uPAR expression and cleavage by uPA and TGF-β1, and the potential implications on migration and invasion ... plasmin (mPLM, 85 kDa), trypsin (24 kDa) and a mixture of both inactive pro-form and active-form of both human MMP-2 monomer (62 and 72 kDa) and MMP-9 monomer (83 and 92 kDa) were used Real-time cell

Ngày tải lên: 06/08/2020, 07:10

... T: Bone morphogenetic protein and transforming growth factor beta inhibitory Smads and are expressed in human adult normal and osteoarthritic cartilage in vivo and are differentially regulated ... for transforming growth factor-beta J Cell Biol 1987, 105:965-975 Massagué J, Like B: Cellular receptors for type beta transforming growth factor Ligand binding and affinity labeling in human and ... transforming growth factor beta signalling pathway genes by transforming growth factor beta in human osteoarthritic chondrocytes: involvement of Sp1 in both early and late response cells to transforming

Ngày tải lên: 10/11/2023, 18:34

... Enhanced expression of transforming growth factor-beta s and transforming growth factor-beta type II receptor in. .. expression in synovial membranes and isolated synovial fibroblasts ... Prechtel, and Mrs Bärbel Ukena for excellent technical assistance and Dr Ernesta Palombo-Kinne for critical reading of the manuscript. We are grateful to Dr Andreas Roth, Dr Rando Winter, and Dr ... TGFB3 Transforming growth factor, beta 3 -0.618 1495_at LTBP1 Latent TGFB binding protein 1 -0.719 37906_at LTBP2 Latent TGFB binding protein 2 -0.751 1957_s_at/32903_at TGFBR1 Transforming growth

Ngày tải lên: 09/08/2014, 10:20

... Ank and PC-1 to transforming growth factor-beta-1 (TGF-β1)-induced increase in extracellular inorganic pyrophosphate (ePPi) productionRespective contributions of Ank and PC-1 to transforming growth ... Effect of transforming growth factor-beta-1 (TGF-β1) on extracellular inorganic pyrophosphate (ePPi) and nucleotide pyrophosphatase phos-phodiesterase (NPPase) activityEffect of transforming growth ... suggested that TGF-β1 Figure 1 Effect of transforming growth factor-beta-1 (TGF-β1) on proteins regu-lating inorganic pyrophosphate metabolismEffect of transforming growth factor-beta-1 (TGF-β1) on proteins

Ngày tải lên: 09/08/2014, 10:22

... each comparison (Tr+ and bleomycin vs Tr- WT and bleomycin; Tr+ and bleomycin vs Tr+; Tr+ vs Tr- WT and bleomycin) A high number of altered genes were found to be upregulated and dowwnregulated ... expression of the TGFβ1 transgene in both line 18 and line 25 Tr+ transgenic mice (Lanes and 2) Lanes and show absence of transgene in wild type mice Figure B and C show PAIL lumineriferase assay results ... luminometer Histological analysis Liver and lung tissue sections were stained with haematoxylin and eosin and Masson's trichrome, which determines collagen deposition and localization Lung fibrosis was

Ngày tải lên: 11/08/2014, 08:21

... endothelial growth factor A (VEGFA), VEGFB, placenta growth factor (PlGF), VEGF receptor (VEGFR1) and VEGFR2, immunohistochemistry and western blots were completed The effects of VEGF ligands on ... assays Real-Time PCR probe and primers name, symbol and ID Gene Symbol Assay ID Vascular endothelial growth factor A Vascular endothelial growth factor B Vegfa Vegfb Placenta growth factor Pgf Rn00585926_m1 ... 3A-C) and hypoxic (figure 3E-3G) lungs, with expression being noted in the alveolar wall and type II pneumocytes (figure 3B and 3F) and the wall of intra-acinar blood vessels (figure 3Cand 3G)

Ngày tải lên: 12/08/2014, 13:22

... activation of TGF-. Airway epithelial cells may produce growth factors such as TGF- and endothelin and epidermal growth fac- tor (EGF)-like growth factors when subjected to mechani- cal stress ... airway wall remodelling and of peripheral neural plasticity in chronic cough, we have measured in bronchoalveolar lavage fluid the levels of growth factors, such as transforming growth factor- (TGF), ... cough, namely growth factors released that induced the remodelling changes, and also that could change cough receptor sensitivity. Release of growth factors such as those of the nerve growth factor

Ngày tải lên: 12/08/2014, 14:20

... pH 4.0 and 7.0 and between 100 and 10 kDa. In the enriched nuclear protein extract, a lower number of protein spots was stained with high intensity and migrated between pH 5.0 and 9.0 and, with ... relations, ontologies and pathways. ResNet 7.0 covers human, mouse and rat proteins. The filters applied included “all shortest paths between selected e ntities” and “expand pathway”.The informa ... proteomes We found 974 and 832 protein spots specifically stained by silver nitrate in HEp-2 cell total and enriched nuclear protein extracts, respectively (Figures 2B and 2E and Additional file

Ngày tải lên: 12/08/2014, 15:23

... colleagues Dr Gunter Maubach and Dr Zhaobing Ding for their useful discussion and help throughout my work I would like to convey my thanks to my colleagues Dr Zhiyuan Ke and Nur-Afidah Bte Mohamed ... their help and comradeship in the lab I would like to express my deep-felt gratefulness to Professor Jackie Ying and Noreena Abubakar, directors of the Institute of Bioengineering and Nanotechnology ... Characteristics of the Hepatic Stellate Cells and their Functions in Normal Liver 1.2.2 Activation of Hepatic Stellate Cells during Liver Injury 1.3 Transforming Growth Factor-1 (TGF-1) 1.4 Gap Junction

Ngày tải lên: 16/10/2015, 11:58

... Ashmawy AM, Zekri AR Transforming growth factor-beta, insulin-like growth factor I/ insulin-like growth factor I receptor and vascular endothelial growth factora: prognostic and predictive markers ... studies are shown in Table and Table Nine studies were conducted in European countries (UK, Germany, Italy, Sweden, Poland, Greece and Bulgaria) and in the United States (US), and four were conducted ... K-ras CRC [6], and bevacizumab for CRC [7], improve survival of patients with CRC over chemotherapy alone [6–8] The transforming growth factor-β (TGF-β) family includes TGF-β1, TGF-β2 and TGF-β3,

Ngày tải lên: 19/09/2020, 21:38

... culture and quick transfection The human EHCC cell lines QBC939 and HuCCT1 used in this study were purchased from American Type Culture Collection (Manassas, USA) and the human IHCC cell line RBE and ... decreased in human EHCC tissues and CC cell lines when compared with the adjacent non-tumor tissues and normal bile duct tissues miR-34a was found correlated with the migration and invasion in EHCC patients ... tumor metastasis and invasion through a variety of signaling pathways in several cancers [10–14] However, the anti-tumor function of miR34a in EHCC is still not clear yet Transforming growth factor-β

Ngày tải lên: 29/09/2020, 16:25

... von Willebrand factor is regulated by vascular endothelial growth factor and related to disease severity Xia Yang1*, Hai-jian Sun1, Zhi-rong Li1, Hao Zhang1, Wei-jun Yang2, Bing Ni1 and Yu-zhang ... Background: von Willebrand factor (vWF) is a potent regulator of angiogenesis, tumor growth, and metastasis Yet, the expression pattern of vWF in human gastric cancer (GC) tissues and its relation ... tumor growth, and angiogenesis [7] von Willebrand factor (vWF), the macromolecular plasma glycoprotein named for its contribution to the hereditary bleeding disorder known as von Willebrand disease

Ngày tải lên: 30/09/2020, 13:55

... obtained from the Queensland Cancer Registery (Australia) in a de-identified and encoded manner Approval to use these samples and data was sought from Dr Glenn Francis and Queensland Health (Australia) ... Queensland University of Technology, Australia (08000 00565), the Princess Alexandra Hospital Australia (2005/ 163), Royal Brisbane & Women’s Hospital, Australia (PR07/004) and Queensland Institute ... immunoreactivity in the normal breast tissues (p < 0.001 and p < 0.001, respectively) and primary cancer tissues (p < 0.01 and p < 0.001, respectively) (Figure 1C and 1A, respectively) Additionally, stromal

Ngày tải lên: 14/10/2020, 15:05

... proliferation and tumor angiogenesis, and participates in tumor cell growth and invasion [1-3] NGF is involved in the development and progression of many tumors of neural origin and epithelial tumors such ... or anthracyclineand taxane-based chemotherapy] and 121 patients received adjuvant endocrine therapy One hundred and ten patients received both chemotherapy and endocrine therapy, and patients received ... suggests NGF and HO1 as tumor-progressing factors However, the correlative role of NGF and HO1 and their prognostic impact in breast carcinoma is unknown Methods: We investigated the expression and prognostic

Ngày tải lên: 05/11/2020, 05:28

... to L donovani survival and growth The collective effect of AKT, ERK, and STAT3 activation, and the generation of polyamines, are likely to lead to growth, proliferation and survival of arginase ... Mlambo NC, Hylander B, Brauner A (1999) Increased levels of transforming growth factor beta and basic fibroblast growth factor in patients on CAPD: a study during non-infected steady state and peritonitis ... growth factor signaling was evident in L donovani infection, and there was an additive effect of IL-4 and growth factors in the induction of arg1 expression, we wanted to know if the FGF-2- and/ or

Ngày tải lên: 02/11/2022, 10:40