Isolation and characterization of highly pathogenic avian influenza virus subtype H5N1 from donkeys docx

... properly cited. Research Isolation and characterization of highly pathogenic avian influenza virus subtype H5N1 from donkeys Ahmed S Abdel-Moneim* 1,2 , Ahmad E Abdel-Ghany 3 and Salama AS Shany 4 Abstract Background: ... host range of the H5N1 influenza virus, possess implications for influenza virus epidemiology and highlight the need for the syste...

Ngày tải lên: 10/08/2014, 05:21

... similarity of the A- and B-chains of the four isoforms, a pairwise alignment was performed and the values of identity expressed in Fig. 3. (Continued). Characterization of four pulchellin isoforms ... M r values of the standards in thousands. Lanes 2–9, 5 lg of peak P I, P II, P III ⁄ P IV and a mixture of other isoforms (*), respec- tively, in the presence (lanes 2–5) o...

Ngày tải lên: 18/02/2014, 16:20

... single stranded DNA ⁄ PEG method as previously described [28]. The expression of Kar2 SP -RTA wild-type and mutant ORFs from the pRS316 vectors was under the control of the GAPDH promoter and the ... permissive temperatures of 30 °C and 37 °C, respectively. A sample of the cells was removed from each culture, rRNAs were isolated in TRIzolÒ (Invitrogen, Paisley, Scotland), a...

Ngày tải lên: 23/03/2014, 07:20

... M: Genetic characterization of the NS gene indicates co-circu- lation of two sub-lineages of highly pathogenic avian influ- enza virus of H5N1 subtype in Northern Europe in 2006. Virus Genes ... K, Cox NJ, Guo Y: Genetic characterization of the pathogenic influenza A/Goose/Guangdong/1/96 (H5N1) virus: similarity of its hemagglutinin gene to those of H5N1...

Ngày tải lên: 20/06/2014, 01:20

... in- oculated with avian influenza viruses of low and high pathogenicity. Avian Dis 1997, 41, 125-136. 12. Naeem K, Naurin M, Rashid S, Bano S. Seroprevalence of avian influenza virus and its relationship ... prevention of disease. References 1. Alexander DJ. The epidemiology and control of avian influ- enza and Newcastle disease. J Comp Pathol 1995, 112, 105-...

Ngày tải lên: 07/08/2014, 20:23

... concentration of 5 ppm to estimate the effect of pH. After 3 min, A 660 was measured and the extent of flocculation was calculated. Pronase digestion of extracellular polymer. Forty milligrams of ... the partially purified polymer contained 309.7 µg of protein, 160.2 µg of neutral sugar, 58.5 µg of hexosamine, 30.8 µg of uronic acid and 3.8 µg of phosphorus. Co...

Ngày tải lên: 05/09/2013, 09:08

... contrary, the N-terminus of PHI is identical to that of MopN from A. calcoaceticus NCIB 8250 and very similar to that of P2 from Pseudomonas CF600, both regulatory components of PHs. PHI has been ... ratio of 1. Asterisks and broken line: data and fitting with PHI/PHO ratio of 2. Fig. 5. Effect of PHI on the flavin fluorescence of the complex PHR– PHO. Dotted line: fluore...

Ngày tải lên: 21/02/2014, 00:20

... intense band of 61 kDa (NrfA) and a band of weak intensity of 19 kDa (NrfH), confirming its hetero-oligomeric nature (Fig. 1, lane 1). However, in the absence of boiling (Fig. 1A, lanes 2 and 4) ... (Calbiochem). Ó FEBS 2003 Characterization of ccNiR subunits (Eur. J. Biochem. 270) 3905 The isolation and characterization of cytochrome c nitrite reductase subunits (NrfA...

Ngày tải lên: 21/02/2014, 00:20

... f®ciencies of two Trx isoforms f and m from spinach, b ecause no T rx f from Chlamydomonas has been isolated until now. Both were active with Ch-Prx1, but while Trx f was as ef®cient as Trx h and m from ... reviously [21]. C hloroplastic thioredoxins m and ffromspinachwereakindgiftofP.Schu È rmann (University of Neuchaà t el, Switzerland). Thioredoxins m and h from Chlamyd...

Ngày tải lên: 08/03/2014, 16:20

... Isolation and characterization of a D-cysteine desulfhydrase protein from Arabidopsis thaliana Anja Riemenschneider, Rosalina Wegele, Ahlert Schmidt and Jutta Papenbrock Institute ... Braun HP (2001) Purification and characteriza- tion of the preprotein translocase of the outer mito- chondrial membrane from Arabidopsis. Identification of multiple forms of TOM20. Plant Ph...

Ngày tải lên: 16/03/2014, 18:20