transcriptional regulation in eukaryotes

... simplex virus thymidine kinase IFN-β, interferon-β Ig, immunoglobulin IgM, immunoglobulin heavy-chain protein IL-2, interleukin-2 IL-12, interleukin-12 Inr, initiator elements int, integrase IPTG, ... biochemical point of view, an understanding of the mechanism of gene regula- tion involves recreating regulated transcription in vitro and delineating the precise pro- tein–protein and protein...

Ngày tải lên: 11/04/2014, 10:37

... containing exons 1 or 1a. To examine pro- moter activity in the 5¢-flanking region of exon 1 in DNASE1, we first obtained the )1386M construct by introducing the )1386 to +268 sequence of DNASE1 into ... lines in RT-PCR amplified fragments A–J indicate regions that are removed by splicing, whereas a dashed line in exon 7 of fragment C represents a dele- tion of 39 bp. The thick lines ind...

Ngày tải lên: 19/02/2014, 06:20

... to act as a defense mechanism in auto-immune diseases. Cross-linking of BCR of pre-mature B-cell lines, including the chicken DT40 cell line, with anti-immunoglobulin IgG induces apoptotic cell death. ... remained unchanged in the presence of PMA/ionomycin. Interestingly, in DT40, PMA/ionomycin treatment increased the caspase-8 mRNA level by 3 h (to approximately 160%) and this level re...

Ngày tải lên: 16/03/2014, 04:20

... Biotechnology Inc., Santa Cruz, CA, USA) against known transcription factors were incubated with the nuclear extract in the presence of 1 · binding buffer at room temperature for 45 min before binding ... are involved in the DNA–protein Complex in TE671 cells. Intrigu- ingly, along with the abolition of Complex A forma- tion by RAR-specific antibody, there was a concomitant increase in...

Ngày tải lên: 16/03/2014, 10:20

... with digoxigenin by using terminal transferase (Roche) according to the manufacturer’s instructions. Binding reactions were carried out in a 20-lL reaction mixture containing 20 mm Tris ⁄ HCl ... box), explaining the regulation of desA expression by binding of DmdR1 to the iron box. The same overlapping was found in S. pilosus. DmdR1 binds to the promoter region of desABCD in both S....

Ngày tải lên: 16/03/2014, 11:20

... the inducer-binding and dimerization domain is in dark gray, activation domains are black boxes, and the silencing domain is stippled. The conformational change leading to the loss of DNA-binding ... and screening systems in E.coli [104,105] and in S. cerevisiae [88] have lead to a profound understanding of how DNA binding, inducer binding and dimerization function in TetR. This infor...

Ngày tải lên: 23/03/2014, 21:20

... partner, cyclin D, are responsible for the initial phosphorylation of the Rb protein by regulating cyclin E/Cdk2 and lifting the S-phase inhibition by preventing binding of its inhibitor, p27 ... 11 distinct Cdks (Cdk1-11) and 11 cyclins (cyclins A–J, T) exist in higher eukaryotes. Cyclin A1, Ccna, was one of the first cyclins demonstrated by targeted disruption in mouse as having a defini...

Ngày tải lên: 25/10/2013, 21:20

... low in England during the nineteenth century, focusing only on lipstick in England could generate a most misleading image of lipstick’s global status. For example, during the same period in China, ... cosmetics 219 Bourjois, Inc., 301 U.S. 183 at 186 (ruling against Bourjois, Inc. on all counts). 220 Id. New York corporation Bourjois, Inc. challenged the Maine cosmetics law in Maine f...

Ngày tải lên: 16/01/2014, 22:20

... moral events were likely to produce intense but conflicting emotions, including some happiness and satisfaction resulting from the gains resulting from victimization, as well as ... 1997). In this model children's reasoning and behavior involving aggressive events are described in terms of six more or less sequential information-processing steps (i.e., encodi...

Ngày tải lên: 19/02/2014, 04:20

... proteins containing a RING– zinc finger domain (data not shown and Fig. 6). Interest- ingly, both a C-terminal deletion mutant lacking the TRAF domain [T4(1–268)–YFP] as well as an N-terminal ... localized in the cytoplasm, the N-terminal deletion mutant, T4(259–470), comprising the TRAF domain of the molecule, and a C-terminal deletion mutant consisting mainly of the RING and zinc finger dom...

Ngày tải lên: 21/02/2014, 15:20