gene targeting protocols

... Biology Methods in Molecular Biology TM TM VOLUME 133 HUMANA PRESS HUMANA PRESS Gene Targeting Protocols Gene Targeting Protocols Edited by Eric B. Kmiec Cationic Lipid Nucleic Acid Transfer 19 30. ... D. D. (1997) Liposomes in Gene Delivery. CRC, Boca Raton, FL. PEI-Based In Vivo Gene Delivery 21 2 21 From: Methods in Molecular Biology, vol. 133: Gene Targeting Protocol...

Ngày tải lên: 10/04/2014, 22:26

... to catalyze homologous targeting is limiting in mammalian cells. For example, gene 118 GENE TARGETING FIGURE 5.2 Strategies of gene targeting. Three prominent options are available in gene targeting. First, ... 1990. 130 GENE TARGETING Templeton NS, Ronerts DD, Safer B. Efficient gene targeting in mouse embryonic stem cells. Gene Therapy 4:700–709, 1997. Thomas KR, Capp...

Ngày tải lên: 10/04/2014, 22:10

... Biology TM Edited by Peter E. Vaillancourt E. coli Gene Expression Protocols VOLUME 205 Edited by Peter E. Vaillancourt E. coli Gene Expression Protocols Cold-Inducible Promoters 7 4. LB medium: ... R. LAVALLIE • Genetics Institute/Wyeth Research, Cambridge, MA H ANS LEHRACH • Max-Planck-Institute for Molecular Genetics, Berlin, Germany; PROT@GEN, Bochum, Germany Z HIJIAN LU • Genetic...

Ngày tải lên: 10/04/2014, 22:36

... Press M E T H O D S I N M O L E C U L A R M E D I C I N E TM Gene Therapy Protocols Edited by Jeffrey R. Morgan Second Edition Gene Therapy Protocols Second Edition Edited by Jeffrey R. Morgan 20 ... capable of targeting genes to hepato- cytes via the asialoglycoprotein receptor. J. Biol. Chem. 272, 7398–7407. 14. Wadhwa, M. S., Knoell, D. L., Young, A. P., and Rice, K. G. (1995) Ta...

Ngày tải lên: 10/04/2014, 22:53

... on a targeting vector and the endogenous genetic locus. When performed in ES cells, gene- targeting is a powerful means for introducing specific genetic mutations into the mouse genome. Gene- targeting ... alt="" Gene Targeting in ES Cells 19 19 From: Methods in Molecular Biology, vol. 158: Gene Knockout Protocols Edited by: M. J. Tymms and I. Kola © Humana Press Inc., Totowa...

Ngày tải lên: 11/04/2014, 00:38

... resulted in levels of reporter gene expression detectable above background levels (13). This com- pares favorably to gene transfer mediated by other DNA-mediated gene trans- fer methods, where ... levels of gene transfer achieved by free viral facilitation of molecular conjugate-mediated gene transfer, adenovirus m the linked configuration is capable of significantly higher lev...

Ngày tải lên: 11/04/2014, 09:42

... need can be achieved either by targeting the vector entry into a specifi c cell subset (transductional targeting) and/or by targeting the expression of the transgene by appropriately regulating ... Biology TM Edited by Maurizio Federico Lentivirus Gene Engineering Protocols VOLUME 229 Edited by Maurizio Federico Lentivirus Gene Engineering Protocols 22 Lotti and Mavilio be pract...

Ngày tải lên: 11/04/2014, 09:49

... proteins or subunits, P and Q, may in general be described by the equilibrium: mP + nQ e PmQn , Lw,,, From Methods m Molecular Bology, Vol 88 Protern Targebng Protocols E&ted by R A Clegg ... sharp, giving very good indication of the n/m of the com- plex. Nonlinear regression analysis is generally used to estimate other binding parameters, followmg standard procedures, typically u...

Ngày tải lên: 11/04/2014, 10:13

... artifact: Generation of heteroduplexes. Am. J. Hum. Genet. 45, 337–339. Mapping Human Genes 25 25 From: Methods in Molecular Medicine, vol. 30: Vascular Disease: Molecular Biology and Gene Therapy Protocols Edited ... Disease: Molecular Biology and Gene Therapy Protocols Edited by: A. H. Baker © Humana Press Inc., Totowa, NJ 2 Analysis of Genetic Variants in Cardiovascular Risk Genes...

Ngày tải lên: 11/04/2014, 10:39