and b cell development using gene targeting technology

Báo cáo y học: "Abnormalities of B cell phenotype, immunoglobulin gene expression and the emergence of autoimmunity in Sjögren’s syndrome" pdf

Ngày tải lên : 09/08/2014, 01:21

... Memory B cells 60% Memory B cells 60% BBcells cells B cells CD27 Expression CD27 Expression Plasmablasts Naïve Naïve B cells B cells Memory Memory B cells B cells CD27 Expression Schematic distribution ... lymphoma exhibited an increase in CD27+ B cells in the blood Figure Normals Sjögren’s ‘s syndrome Plasmablasts Plasmablasts Naïve Naïve B cells ≥80% Memory Memory B cells 80% B cells Systemic ... and CD70 expressed by B cells only [77] was also reported to be sufficient for B cell differentiation, raising the possibility that B cells might be able to regulate themselves by CD27–CD70 interactions...

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Báo cáo y học: "No evidence for an association between the -871 T/C promoter polymorphism in the B-cell-activating factor gene and primary Sjögren''''s syndrome" pptx

Ngày tải lên : 09/08/2014, 07:20

... by χ2 testing The association between BAFF polymorphism and BAFF protein or mRNA level was analyzed using the Mann–Whitney U test The association between BAFF polymorphism and serum gammaglobulin, ... autoantibody: 52%; P = 0.76) No association was observed between BAFF gene polymorphism and mean serum gammaglobulin, IgG, or rheumatoid factor levels assessed by nephelometry (gammaglobulin, IgG, and ... not influence BAFF level Surprisingly, no correlation was observed between BAFF polymorphism and BAFF mRNA levels in patients with pSS The absence of concordance between BAFF protein and mRNA regarding...

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Multiple view product representation and development using augmented reality technology

Ngày tải lên : 11/09/2015, 10:06

... consistency of the feature entities between the server and the client WebSPIFF [Bidarra and Bronsvoort 2000, van den Berg et al 2000, Bidarra et al 2002] is a web-based collaborative modeling system that ... Recently, there have been active research and developments of software tools and methodologies to support distributed and collaborative design These collaborative design systems can be generally classified ... product In an AR-based environment, which is a synthetic environment, real objects can be viewed and manipulated by the users and hence tangible interaction can be performed Tangible interfaces...

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Báo cáo y học: "B-lymphocyte stimulator/a proliferation-inducing ligand heterotrimers are elevated in the sera of patients with autoimmune disease and are neutralized by atacicept and B-cell maturation antigen-immunoglobulin" pdf

Ngày tải lên : 12/08/2014, 12:20

... activity of BLyS, APRIL, and the recombinant BLyS/APRIL heterotrimer in the TACI-Jurkat and human B cell- proliferation assays, whereas BAFF-R-Ig inhibited BLyS but exhibited little or no inhibition ... followed by donkey anti-rabbit IgG-HRP for the trypsinized form of APRIL The anti-BLyS blot comprised anti-6x His HRP for the Hisx6-zz12.6 form of BLyS, and anti-BLyS pAb followed by donkey anti-rabbit ... interactor (TACI), B cell- maturation antigen (BCMA), and BAFF receptor (BAFF-R), whereas APRIL homotrimers bind to TACI, BCMA, and proteoglycans [7,8] The binding of BLyS and APRIL to these receptors...

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Báo cáo khoa học: Nuclear factor kappa B and tumor necrosis factor-alpha modulation of transcription of the mouse testis- and pre-implantation development-speciﬁc Rnf33⁄Trim60 gene pot

Ngày tải lên : 29/03/2014, 00:20

... regulated by an intronic jB sequence modulated by the NF-jB subunits p65 and p50, and also by TNF-a Although p65 is probably expressed in the oocyte and the pre-implantation embryo, it remains to be ... the p65 and p50 NF-jB subunit proteins in testicular cells (A) RT-PCR detection of p50 and p65 transcripts in the testis (Te) and in TM3 and TM4 cells (B) Western blot analysis of the p50 and p65 ... appreciably affect the protein-shifted band in both TM3 and TM4 cells (Fig 6B, lanes and 5) In in vivo p65-binding assays carried out by chromatin immunoprecipitation (ChIP), the anti-p65 and -p50...

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Báo cáo hóa học: " Quantitative profiling of housekeeping and Epstein-Barr virus gene transcription in Burkitt lymphoma cell lines using an oligonucleotide microarray" docx

Ngày tải lên : 20/06/2014, 01:20

... EBV genes: some from the latent phase (e.g., EBNA1, EBNA2, EBNA3A, EBNA3C, LMP1, and LMP2) and some from lytic EBV infection (e.g., BZLF1/ Zta, BXLF2/gp85, BKRF2/gL and BZLF2/gp42) (Table 1B) ... used B9 5.8 RNA as positive control and RNA from the EBV-negative BL cell line BJAB as a negative control to eliminate probes that cross-hybridize with cellular genes (Fig 1) RNAs from B9 5.8 and BJAB ... BJAB cells were compared in self-vs-self experiments EBV probes giving a signal with B9 5.8 RNA but not with BJAB RNA were selected We tested three probes for EBNA1, EBNA2, and BXLF2/gp85 and...

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báo cáo hóa học:" Development and evaluation of a clinical algorithm to monitor patients on antiretrovirals in resource-limited settings using adherence, clinical and CD4 cell count criteria" ppt

Ngày tải lên : 20/06/2014, 08:20

... 2009, 12:3 CD4 cell count was measured by change in CD4 cell count from baseline, 30% fall and 50% fall from maximum achieved, persistent CD4 cell count of CD4 cell count at ... oversight and conduct and manuscript writing LS and LJ performed data analysis and contributed to manuscript writing RD performed laboratory analyses and contributed to manuscript writing HT, SM and ... WHO-based algorithm (Figure 1b) , patients with immunologic failure and Stage disease (not including lymph node TB, uncomplicated TB pleural disease, oesophageal candidiasis, and recurrent bacterial...

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Báo cáo y học: "Molecular processes during fat cell development revealed by gene expression profiling and functional annotation" pptx

Ngày tải lên : 14/08/2014, 16:20

... Correspondence between coexpression and targeting by microRNAs between the small RNA and the mRNA determines the balance between cleavage and degradation [26] Rules for matches between miRNA and target ... B- cell translocation gene 1, anti-proliferative (Btg1) Eph receptor B6 (Ephb6) procollagen, type III, alpha (Col3a1) DnaJ (Hsp40) homolog, subfamily B, member (Dnajb9) RIKEN cDNA 0610039N19 gene ... Transcription of actin α (Acta1 [number 445, cluster 10]) and actin γ (Actg1 [number 656, cluster 10]), tubulin α (Tuba4 [number 377, cluster 8]), and tubulin β (Tubb5 [number 110, cluster 8]) were found...

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Establishing improved platforms for dengue diagnosis and hybridoma development using dengue virus envelope domain III antigen b

Ngày tải lên : 10/09/2015, 15:53

... (IB4C-2, 4A5C-8, 2B3 A-1 and 9A4D-1) and II ( 6B6 C-1, 4G2, 4E5, 1A5D-1, 1B7 and 10A1D-2) have been shown to be able to block virus entry into Vero cells (approx 18% to 46% blocking), but not as strongly ... consistent and fair comparison between both (MA-based and packed-bed) puriﬁcation procedures, the cell lysate to (His-tagged protein) binding capacity ratio was maintained equal between both methods ... USA) The membrane was washed and subsequently developed using BCIP/ NBT substrate (Millipore, USA) Packed-bed chromatography using Ni–NTA Agarose (Qiagen, Germany) was performed and optimized...

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Elucidating the role of dok 3 in b cell receptor signaling using gene knockout mice 2

Ngày tải lên : 14/09/2015, 09:08

... SC-7383 3941 Goat Goat Rabbit Rabbit Rabbit Rabbit Rabbit Mouse Rabbit 1:500 1:500 1:500 1:500 1:500 1:1000 1:500 1:500 1:500 Cell Signaling Cell Signaling 9211 9251 Rabbit Rabbit 1:500 1:500 Upstate ... cell cycle Moreover, Bcr-Abl inhibits cellular DNA repair, therefore causing genomic instability and increase in cell susceptibility to developing further genetic abnormalities Indeed, Bcr-Abl ... circulation B- 2 cells express high levels of B2 20, IgD and CD23 and moderate levels of IgM and lack surface CD5 expression The B- 1 population can be further subdivided into B- 1a and B- 1b subpopulations...

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Chapter 2 literature review design and development of tissue engineering scafflods using rapid prototyping technology

Ngày tải lên : 14/09/2015, 10:20

... was synthesized by combining both PEG and PLA blocks with PCL chains to produce novel hydrophilic and bioresorbable co-polymer with the aim of enhancing hydrophilicity and degradability Similarly, ... layer (Paul and Baskaran, 1996; Leong et al, 2003a and 200 3b) The object is embedded and supported by the surrounding unprocessed powders during fabrication, and extracted from the powder bed upon ... engineering scaffold fabrication, there is a limited choice of photopolymerisable biomaterials that have the required biodegradability and biocompatibility, mechanical stability and other prerequisite...

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Chapter 4 results discussions design and development of tissue engineering scafflods using rapid prototyping technology

Ngày tải lên : 14/09/2015, 10:23

... trabecular and cancellous bones DRBRP technique therefore, has high potential to be a flexible technique that enabled scaffolds to be designed and developed with various macrostructures suitable ... designed and fabricated using DRBRP system was highly similar to the honeycomb of a bee (Gibson and Ashby, 1997), with its regular array of identical pores, when viewed in the Z-direction of the fabrication ... deformation least causing the scaffold most deformable This can also be explained by mathematical model developed by Gibson and Ashby (1997) for cellular solids The compressive stiffness E and yield strength...

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Chapter 5 conclusions recommendations design and development of tissue engineering scafflods using rapid prototyping technology

Ngày tải lên : 14/09/2015, 10:23

... as well as the ability of the PCL and PCL-PEG scaffolds to favour cell adhesion and function was evaluated successfully using rabbit smooth muscle cells Light, scanning electron, and confocal laser ... can be developed with a range of morphological and biomechanical properties by various selections of design and process parameters in combination with different polymers using the in house built ... processing demonstrated in one hand, the efficacy of the DRBRP technique to process a wide range of biopolymers and on the other hand, the feasibility of these polymers to be processed into 3D scaffolds...

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Elucidating the role of dok 3 in b cell receptor signaling using gene knockout mice

Ngày tải lên : 14/09/2015, 10:44

... highlighted using Dok-3 deficiency DT40 chicken B cell line (Stork et al, 2007) ix Abbreviations Abl AChR BCR BLNK BMDM Btk CML Csk DAG DNA Dok ERK ES FACS FITC FRIP G3BP G418 GANC Grb2 HA I(1,3,4,5)P4 ... G3BP-1 and Table Summary of Dok family members Table Enumeration of total and lymphocytes populations in various tissues of wild-type and Dok-3 -/- mice Table Enumeration of total cells and B2 20+ ... with G3BP-1 and G3BP-2 xii List of Schematic Diagrams and Tables Diagram B cell receptor signaling pathway Diagram SHIP-1 mediated inhibition of cellular activation Diagram Structure and domains...

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Design and development of tissue engineering scafflods using rapid prototyping technology chapter 3

Ngày tải lên : 14/09/2015, 11:12

... distribution was obtained by direct 3D measurement using a distance transformation technique (Hildebrand and Rüegsegger, 1997), whereby the number of voxels within the total PV that could be filled ... from male New Zealand White Rabbit The rabbit was maintained under general anaesthesia by intubation with isofluorane The local area was cleaned with iodine solution and alcohol, and was opened ... The distribution and ratio of viable and dead cells into the cell- scaffold constructs were observed with a confocal laser microscope (CLM) (Zeiss LSM510 META, Germany) on week and Viable cells were...

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Design and development of tissue engineering scafflods using rapid prototyping technology

Ngày tải lên : 14/09/2015, 11:12

... nutrients and metabolic waste, and tissue integration and vascularization b) Biodegradable or bioresorbable with a controllable degradation and resorption rate to match cell/ tissue growth in vitro and/ or ... study, human fibroblasts are used to assess the general biocompatibility of PEG-PCL-PLA scaffolds Further, rabbit smooth muscle cells are cultured to evaluate biocompatibility and cellular functions ... pores, ridges, fibres and networks Fundamental understanding of interactions between cells and their living environments, such as extracellular matrix, is critical for design and development of...

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$Development and characterization of refractive solid immersion lens technology for far field integrated circuit faillure analysis using laser induced techniques$

Development and characterization of refractive solid immersion lens technology for far field integrated circuit faillure analysis using laser induced techniques

Ngày tải lên : 30/09/2015, 06:19

... centric location using variable backing objectives and 68% clear aperture Change in spatial resolution with distance from centric location using variable backing objectives and 95% clear aperture ... laser and (a) 20X objective and (b) 50X objective Regions A and B denote the areas where the mobile DSIL will be positioned Reflected laser image of subsurface features captured using 20X 142 145 ... has generated interest because of applications in biological sciences imaging, tomography and imaging though Si substrate in backside analysis Ling and Lee [67] treated the problem first and subsequently,...

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Dissecting gene regulatory networks in vertebrate development using genomic and proteomic approaches

Ngày tải lên : 04/10/2015, 16:03

... of ES cell colonies of both wild type and Oct4-2xflag-TEV-BAP clones 78 4.5 Screening for Oct4-2xflag-TEV-BAP: Blot probed with antiflag 79 4.6 Screening for Oct4-2xflag-TEV-BAP: Blot probed with ... Oct4-2xflag-TEV-BAP: Blot probed with streptavidin-HRP 80 4.8 Screening for Oct4-2xflag-TEV-BAP: Blot probed with antiOct4 81 4.9A Screening for Oct4-pre-flag-TEV-BAP: Blot probed with antiflag 82 4. 9B Screening ... Oct4-pre-flag-TEV-BAP: Blot probed with antiflag 82 4.10A Screening for Oct4-pre-flag-TEV-BAP: Blot probed with antiEGFP 83 4.1 0B Screening for Oct4-pre-flag-TEV-BAP: Blot probed with antiEGFP...

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Gene targeting in human pluripotent cell derived neural stem cells for the study and treatment of neurological disorders

Ngày tải lên : 26/11/2015, 09:54

... England Biolabs Frankfurt, Germany New England Biolabs Frankfurt, Germany New England Biolabs Frankfurt, Germany New England Biolabs Frankfurt, Germany New England Biolabs Frankfurt, Germany Cell ... disruption of both alleles of the ADK and subsequent differentiation into neural precursor cells by the laboratories of Brüstle and Boison (Fedele et al., 2004; Li et al., 200 7b) These cells were ... fibroblasts are not a natural population of the brain, so a neural cell type with a specifically altered genotype was demanded This was achieved by engineering murine embryonic stem cells by genetic...

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Prediction of deformation and hygro-thermal stresses distribution in PEM fuel cell vehicle using threedimensional CFD model

Ngày tải lên : 05/09/2013, 14:58

... durability in a PEM fuel cell studied using PVDF based radiation grafted membranes fuel cells Fuel Cells 2003; 3(1-2): 21-27 Maher A.R Sadiq Al-Baghdadi Mechanical behaviour of PEM fuel cell catalyst layers ... part of the fuel cell components, which is the polymer membrane An operating fuel cell has varying local conditions of temperature, humidity, and power generation (and thereby heat generation) across ... protons, and energy Water transport inside the porous gas diffusion layer and catalyst layer is described by two physical mechanisms: viscous drag and capillary pressure forces, and is described by...

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